Effects of acrous gramimeus and its main component alpha-asarone on the reactivity and convulsive threshold of immature rats to electric stimulation

BACKGROUND： The traditional Chinese medicine acrous gramimeus is the dry rhizome of Acrous gramimeus Soland, a kind of Araceae familial perennial herb, which has a sedation action, anticonvulsant and antiepileptic effect. Its effective component has not been known yet, and α-asarone, the major component of the volatile oil extracted from acrous gramineus, has been supposed to play a necessary role in it. OBJECTIVE： To explore the effects of acrous gramimeu and α-asarone on the reactivity and convulsive threshold to electric stimulation in immature rats, furthermore, attempt to definitize the anticonvulsant effect of α-asarone. DESIGN： A randomized controlled study.SETTINGS： Department of Pediatrics, First Hospital of Jilin University; Department of Histology and Embryology, School of Basic Medical Sciences of Jilin University; Department of Neurology, First Clinical Hospital affiliated to Harbin Medical University; Department of Internal Medicine, Children＇s Hospital of Changchun City. MATERIALS ： Seventy 3-week immature Wistar rats （either males or females） of 34-40 g were used. Acrous gramimeu （1 g/bag, the content of α-oasarone was 0.046 26%-0.070 16%） with the batch number of 0307113 was provided by Tianjiang Medicine Company Limited, Jiangyin City. α-asarone tablet （60 mg per tablet） with the batch number of 030219 was provided by Tianwei Pharmaceutical Factory, Shenyang City. α-asarone injectable preparation （2 mL per piece） with the batch number of 030105 was provided by Shuanghe Medicine Limited Company, Beijing City. METHODS ： The experiments were carried out in the Neurological Laboratory of the First Hospital of Jilin University between August and October in 2004.① The 70 rats were randomly divided into intragastric subset and intraperitoneal subset. The intragastric subset included four groups of control, phenobarbital sodium, acrous gramimeu and α-asarone; the intraperitoneal subset included three groups of control, phenobarbital sodium and α-asarone. There were 10 rats per group. ② In the intragastric subset, different group was treated with saline （1 mL for each time, phenobarbital sodium （18 mg/kg per day）, acrous gramineu （2 350 mg/kg per day） and α-asarone （29 mg/kg per day） respectively twice every day for 5 days. In the intraperitoneal subset, different group was treated with saline （0.5 mL）, phenobarbital sodium （29 mg/kg） and α-asarone （2.9 mg/kg） respectively. ③ Before and after administration for 5 days in the intragastric subset as well as before and after administration for about 1 hour in the intraperitoneal subset respectively, the rats were given electric stimulation with the NIHOM KOMDEM multifunctional electrophysiological recorder, and the reactivity and convulsive threshold to electric stimulation of the rats were recorded. MAIN OUTCOME MEASURES： The reactivity and convulsive threshold to electric stimulation in immature rats were compared. RESULTS： All the rats were involved in the analysis of results. ① Results for intragastric administration： Before intragastric administration, there were no obvious differences in the reactivity and convulsive threshold to electric stimulation among the groups （P 〉 0.05）. After intragastric administration for 5 days, the reactivity and convulsive threshold to the electric stimulation had no obvious changes in the control group, but those were significantly higher than before administration in the drug administration groups （t=-3.317-7.401, P 〈 0.01）, which were also obviously higher than those in the control group （t=3.027-8.941, P 〈 0.01）, and those in the acrous gramimeu group and α-asarone group were not markedly different from those in the phenobarbital sodium group. ② Results for intraperitoneal injection： Before intraperitoneal injection, the reactivity and convulsive threshold to the electric stimulation had no obvious differences among the groups. After the intraperitoneal injection for 1 hour, the reactivity and convulsive threshold to the electric stimulation had no obvious change in the control group, but those were significantly higher than before administration in the drug administration groups （P 〈 0.01）, which were also obviously higher than those in the control group （t=6.211-7.237, P 〈 0.01; t=4.085-5.633, P 〈 0.05）, and there was no marked difference between α-asarone group and phenobarbital sodium group （P 〉 0.05）.CONCLUSION ： ① As effective anticonvulsants, both acrous gramineu and α-asarone can enhance the reactivity and convulsive threshold of immature rats to electric stimulation. ② As one of the major effective components against convulsion of acrous gramineu, α-asarone is equivalent to phenobarbital sodium.

Main Components of the WB’s Reform Package

1. Financial Resources: ● an increase of $86.2 billion in capital for the International Bank for Reconstruction and Development (IBRD), and ● a $200-million increase in the capital of the International Finance Corp. (IFC), the World Bank Group’s private sector arm. 2. Voting Power: ● a 3.13-percentage point increase in the voting power of Developing and Transition countries (DTCs)

Research Progress of Action Mechanism of Mongolian Medicine Hatageqi-7 in the Treatment of Recurrent Aphthous Ulcer

Based on Mongolian medical theory and modern pharmacology,literature on the main components and action mechanism of Hatageqi-7 in the treatment of recurrent aphthous ulcer is collected,sorted and summarized.The research hopes to provide certain basis for its clinical efficacy from theory.

卟啉类光敏药物主要活性组份对溶菌酶的光敏杀伤特性

研究了卟啉类光敏药物主要活性组分：原卟啉（ＰＰ）、血卟啉（ＨＰ）及双卟啉醚、酯混合物（ＤＨＥ）对Ｈｅｌａ细胞（宫颈癌）的光敏杀伤作用特性，首次发现ＰＰ对癌细胞杀伤力最大，是最有效的卟啉组分。进而研究了其作用的分子机制，采用激光喇曼光谱技术，研究了三种组分对溶菌酶空间结构的光敏损伤。结果表明，它们对溶菌酶主链和侧链结构的损伤明显不同：ＰＰ对蛋白质主链结构中的β—折叠，β—回折的光敏损伤明显强于ＨＰ和ＤＨＥ且不随浓度而变．

Research on Global Higher Education Quality Based on BP Neural Network and Analytic Hierarchy Process

Having a universal, fair, democratic and practical higher education system plays a particularly important role in the future development of the country. However, the higher education system in various countries is uneven. It is of great significance to establish a general evaluation system for the development of global education. In this paper, 23 indicators are preliminarily selected from the education data of Universitas 21 and Global Statistical Yearbook. After the gray correlation analysis, 12 indicators were selected. On the one hand, principal component analysis is used to reduce the dimension of these 12 indicators in 50 countries, and the first four principal components with cumulative contribution rate of 99% are finally selected as the input parameters of BP neural network. On the other hand, 12 indicators are divided into four aspects as the standard of scheme decision-making. Finally, a higher education quality evaluation and decision-making model based on BP neural network and analytic hierarchy process are established. Then eight countries are selected to use the model to evaluate their current higher education quality. Based on the input and evaluation results of the four aspects of higher education in various countries, the analytic hierarchy process is used to make program decision, and several improvement suggestions are put forward for the current education policies of various countries.