Southern corn leaf blight(SCLB)disease caused by Cochliobolus heterostrophus is one of the major threats to corn production worldwide.The synergistic application of low toxic chemical fungicide and biocontrol agents c...Southern corn leaf blight(SCLB)disease caused by Cochliobolus heterostrophus is one of the major threats to corn production worldwide.The synergistic application of low toxic chemical fungicide and biocontrol agents could improve biocontrol stability and efficiency against plant diseases,which ultimately reduce use of chemical fungicide.Trichoderma spp.,well-known biocontrol fungi have been used to control some foliar diseases.However,few works have been reported on synergistic application of chemical fungicide and Trichoderma against foliar diseases.This study was aimed to investigate the control effect on the synergistic application of Trichoderma harzianum SH2303 and difenoconazole-propiconazole(DP)against SCLB.Results showed that the synergistic application of DP and SH2303 reduced the leaf spot area compared to the control.The efficacy of synergistic application of DP+SH2303 against SCLB could last for 15–20 d in pot trial under the greenhouse condition.Under the natural field condition,maize treated with DP+DP and DP+SH2303 showed 60%control,which was higher than that of SH2303+DP(45%)and SH2303+SH2303(35%).All these treatments induced the synthesis of defense-related enzymes(phenylalanine ammonia lyase(PAL),catalase(CAT),and superoxide dismutase(SOD))and the defence-related gene expression of SA pathway(PR1).Taken together the in-vitro leaf test and field trial,the control of SCLB by synergistic application of DP+SH2303 was similar to that of DP+DP.Among synergistic application,the sequential application of DP+SH2303 showed better control than the sequential application of SH2303+DP.It was concluded that the synergistic application of chemical fungicide(DP)and biocontrol agent(T.harzianum SH2303)could be used to reduce the chemical fungicide and to reduce the SCLB diseases in maize,which provided alternative approach to realize an eco-friendly controlling of the foliar disease.展开更多
采用双因素试验设计,分别采用4种不同药剂、3种不同施药次数开展对玉米灰斑病和大斑病药效防控试验。结果表明,防控玉米灰斑病和大斑病施用药剂以A2即苯甲·丙环唑(水乳剂)为最优,一次施药即可,通过防控病害能达到较好的增产效果。...采用双因素试验设计,分别采用4种不同药剂、3种不同施药次数开展对玉米灰斑病和大斑病药效防控试验。结果表明,防控玉米灰斑病和大斑病施用药剂以A2即苯甲·丙环唑(水乳剂)为最优,一次施药即可,通过防控病害能达到较好的增产效果。药剂A1即杜帮·克露(杀菌剂)和药剂A3即三唑酮(可湿性粉剂)施药3次也能较好地防控灰斑病,但对大斑病防治效果不佳,有一定增产效果。实际生产中推荐使用药剂A2即苯甲·丙环唑(水乳剂)、用量750 m L/hm2(有效成分苯醚甲环唑含量15%、丙环唑含量15%),在玉米植株抽雄后及时施药1次。展开更多
基金supported by the National Key Research and Development Program of China (2017YFD0201108, 2017YFD0200901)the National Natural Science Foundation of China (31672072, 31872015, 31750110455)+2 种基金the earmarked fund for China Agriculture Research System (CARS-02)the Key National R&D Programs of China-Key International Intergovernmental Scientific and Technological Innovation Cooperation Projects (2017YFE0104900)the Agriculture Research System of Shanghai, China (201710)
文摘Southern corn leaf blight(SCLB)disease caused by Cochliobolus heterostrophus is one of the major threats to corn production worldwide.The synergistic application of low toxic chemical fungicide and biocontrol agents could improve biocontrol stability and efficiency against plant diseases,which ultimately reduce use of chemical fungicide.Trichoderma spp.,well-known biocontrol fungi have been used to control some foliar diseases.However,few works have been reported on synergistic application of chemical fungicide and Trichoderma against foliar diseases.This study was aimed to investigate the control effect on the synergistic application of Trichoderma harzianum SH2303 and difenoconazole-propiconazole(DP)against SCLB.Results showed that the synergistic application of DP and SH2303 reduced the leaf spot area compared to the control.The efficacy of synergistic application of DP+SH2303 against SCLB could last for 15–20 d in pot trial under the greenhouse condition.Under the natural field condition,maize treated with DP+DP and DP+SH2303 showed 60%control,which was higher than that of SH2303+DP(45%)and SH2303+SH2303(35%).All these treatments induced the synthesis of defense-related enzymes(phenylalanine ammonia lyase(PAL),catalase(CAT),and superoxide dismutase(SOD))and the defence-related gene expression of SA pathway(PR1).Taken together the in-vitro leaf test and field trial,the control of SCLB by synergistic application of DP+SH2303 was similar to that of DP+DP.Among synergistic application,the sequential application of DP+SH2303 showed better control than the sequential application of SH2303+DP.It was concluded that the synergistic application of chemical fungicide(DP)and biocontrol agent(T.harzianum SH2303)could be used to reduce the chemical fungicide and to reduce the SCLB diseases in maize,which provided alternative approach to realize an eco-friendly controlling of the foliar disease.
文摘采用双因素试验设计,分别采用4种不同药剂、3种不同施药次数开展对玉米灰斑病和大斑病药效防控试验。结果表明,防控玉米灰斑病和大斑病施用药剂以A2即苯甲·丙环唑(水乳剂)为最优,一次施药即可,通过防控病害能达到较好的增产效果。药剂A1即杜帮·克露(杀菌剂)和药剂A3即三唑酮(可湿性粉剂)施药3次也能较好地防控灰斑病,但对大斑病防治效果不佳,有一定增产效果。实际生产中推荐使用药剂A2即苯甲·丙环唑(水乳剂)、用量750 m L/hm2(有效成分苯醚甲环唑含量15%、丙环唑含量15%),在玉米植株抽雄后及时施药1次。