帕金森病患者血清NPASDP-4,MBP水平表达与认知功能障碍及严重程度的诊断价值研究

目的探讨帕金森病患者血清神经元PAS结构域蛋白4(neuronal Per-Arnt-Sim domain protein 4,NPASDP-4)、髓鞘碱性蛋白(myelin basic protein,MBP)水平表达与认知功能障碍(cognitive impairment,CI)及严重程度的诊断价值研究。方法选取中国人民解放军联勤保障部队第九〇九医院收治的138例帕金森病患者为帕金森病组,同期该院体检中心的健康体检者69例为健康对照组,并根据是否发生CI以及其严重程度进一步将帕金森病组患者分为认知功能正常组(n=55)、轻度CI组(n=51)和痴呆组(n=32)。收集受试者一般资料;ELISA法检测血清NPASDP-4和MBP水平;相关性分析采用Spearman等级相关或Pearson线性相关;诊断价值分析采用ROC曲线;影响因素分析采用多因素Logistic回归。结果与健康对照组比较,帕金森病组血清NPASDP-4(6.75±0.48ng/ml vs2.38±0.31ng/ml),MBP(8.34±0.65μg/L vs 3.54±0.42μg/L)水平升高,差异具有统计学意义(t=68.751,55.761,均P<0.05)。认知功能正常组、轻度CI组、痴呆组H-Y分期比较,差异有统计学意义(χ2=7.788,P<0.05)。UPDRS-Ⅲ评分与认知功能正常组(41.95±10.36分)比较,轻度CI组(47.92±11.63分)、痴呆组(50.78±13.69分)评分升高,差异具有统计学意义(H=6.672,均P<0.05)。认知功能正常组、轻度CI组、痴呆组病程(4.28±0.54,4.71±0.58和5.16±0.63年)及血清NPASDP-4(5.89±0.40,6.83±0.55和8.12±0.54ng/ml),MBP(6.65±0.56,8.94±0.69和10.27±0.70μg/L)水平依次显著升高(H=24.114,207.950,355.594,均P<0.05),MMSE评分(28.47±0.94,24.51±1.35和17.09±2.57分)、MoCA评分(27.45±1.03,20.18±1.92和11.75±2.53分)、GPCOG总分(13.47±0.69,10.25±1.04和8.97±0.82分)依次显著降低(H=515.005,775.933,327.584,均P<0.05),差异具有统计学意义。帕金森病患者血清NPASDP-4,MBP水平均与病程(r=0.316,0.358)、H-Y分期(r=0.345,0.384)、UPDRS-Ⅲ评分(r=0.371,0.396)呈显著正相关(P<0.05),与MMSE评分(r=-0.468,-0.517)、MoCA评分(r=-0.504,-0.569)、GPCOG总分(r=-0.527,-0.538)呈显著负相关(均P<0.05)。血清NPASDP-4,MBP水平及二者联合诊断帕金森病患者CI的曲线下面积(AUC)分别为0.850,0.930和0.960,诊断帕金森病患者CI严重程度的AUC分别为0.866,0.803和0.933。H-Y分期中期[OR(95%CI):4.725(1.742~12.814)],H-Y分期晚期[OR(95%CI):5.083(1.919~13.464)]、UPDRS-Ⅲ评分[OR(95%CI):3.257(1.464~7.246)]、NPASDP-4[OR(95%CI):5.324(1.516~18.701)]和MBP[OR(95%CI):5.769(2.459~13.533)]是帕金森病患者CI的影响因素(均P<0.05);NPASDP-4[OR(95%CI):4.768(2.382~9.543)],MBP[OR(95%CI):5.846(3.141~10.882)]是帕金森病患者CI严重程度的影响因素(均P<0.05)。结论帕金森病患者血清NPASDP-4和MBP呈高水平,且均与CI及其严重程度密切相关,可能具有一定的临床诊断价值。

卒中后癫痫患者的血清MBP、Nesfatin-1表达水平及其危险因素

目的观察卒中后癫痫患者的血清髓鞘碱性蛋白(MBP)、新饱食分子蛋白-1(Nesfatin-1)表达水平,并探讨其危险因素。方法回顾性分析2021年7月至2023年7月于肇庆市第一人民医院神经内科住院治疗的200例卒中患者临床资料,将卒中后发生癫痫的85例患者作为研究组,未发生癫痫的115例作为对照组。比较两组患者的年龄、性别、吸烟、饮酒、卒中部位、病灶范围、病灶大小、基础疾病、美国国立卫生院卒中量表(NIHSS)评分和血清MBP、Nesfatin-1水平,并采用Logistic回归分析影响卒中后癫痫的危险因素。结果两组患者的年龄、性别、吸烟、饮酒、基础疾病比较差异均无统计学意义(P>0.05);研究组患者卒中部位为皮质、病灶范围为多脑叶、病灶>5 cm2、NIHSS评分>20分的占比明显高于对照组,差异均有统计学意义(P<0.05);研究组患者的MBP、Nesfatin-1水平分别为(5129.17±211.18)pg/mL、(7.30±1.83)mg/mL,明显高于对照组的(3135.10±150.60)pg/mL、(3.66±0.78)mg/mL,差异均有统计学意义(P<0.05);Logistic回归分析结果显示,血清MBP、Nesfatin-1、卒中部位、病灶范围、病灶大小、NIHSS评分均是影响卒中后癫痫患者的危险因素(P<0.05)。结论血清MBP、Nesfatin-1、卒中部位、病灶范围、病灶大小、NIHSS评分均是影响卒中后癫痫患者的危险因素,需要对这些危险因素进行临床干预,以减少卒中后癫痫的发生率。

MECT联合阿立哌唑治疗精神分裂症的效果及对MBP、GDNF和炎症因子的影响

目的:探讨无抽搐电休克治疗(MECT)联合阿立哌唑治疗精神分裂症的效果及对髓鞘碱性蛋白(MBP)、胶质细胞源性神经营养因子(GDNF)和炎症因子的影响。方法:选取2020年1月—2022年12月咸宁博德精神病医院收治的100例精神分裂症患者。根据随机数表法将其分为观察组与对照组,各50例。对照组给予阿立哌唑治疗,观察组在对照组基础上给予MECT治疗。比较两组治疗前后精神分裂症症状、相关指标、炎症因子,临床疗效及不良反应。结果:治疗后,两组阴性症状、阳性症状、一般精神病理症状评分及总分显著降低,观察组阴性症状、阳性症状评分及总分均显著低于对照组,差异有统计学意义(P<0.05)。观察组总有效率显著高于对照组,差异有统计学意义(P<0.05)。治疗后,两组MBP水平显著降低,GDNF水平显著增高,观察组MBP水平低于对照组,GDNF水平高于对照组,差异有统计学意义(P<0.05)。治疗后,两组肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)水平显著降低,观察组TNF-α、IL-1β均低于对照组,差异有统计学意义(P<0.05)。两组不良反应发生率比较,差异无统计学意义(P>0.05)。结论:MECT联合阿立哌唑治疗精神分裂症能够更好地调节MBP、GDNF表达和炎症反应,改善患者精神症状,提高临床疗效,且不增加不良反应。

缺血性脑卒中SD大鼠血清、脑脊液中NSE、MBP水平及其与神经功能缺损评分和脑梗死体积的相关性

目的探讨缺血性脑卒中SD大鼠血清、脑脊液中神经元特异性烯醇化酶(NSE)、髓鞘碱性蛋白(MBP)水平及其与神经功能缺损评分和脑梗死体积的相关性。方法购买SD大鼠进行大脑中动脉闭塞缺血模型造模手术,将术后成功造模的大鼠随机分为8组,每组8只,术后8 h进行神经功能缺损评分,术后在8 h、12 h、1 d、3 d、5 d、7 d及21 d时间段采集腹腔静脉血及抽取延髓脑脊液后,断头取脑,进行0.4%的2,3,5-氯化三苯基四氮唑大脑染色测定脑梗死体积,并检测NSE及MBP水平。采用Spearman相关对血清、脑脊液NSE、MBP水平与脑梗死体积和神经功能缺损评分的相关性进行分析。结果各组血清、脑脊液中NSE和MBP水平在术后8 h开始升高,3 d达峰值,7 d下降至与对照比较差异无统计意义(P>0.05);术后8 h至5 d,脑脊液NSE和MBP水平明显高于血清。血清、脑脊液NSE、MBP水平与脑梗死体积、神经功能缺损评分均呈正相关(P<0.05)。结论血清、脑脊液中NSE、MBP为脑梗死的新型标志物,通过动物实验可为临床患者标本采集推荐最佳时间窗口。

血清S-100βNSEMBP动态变化对新生儿颅内出血预后的评估价值

目的探究血清S-100β蛋白(S-100β)、神经元特异性烯醇化酶(NSE)、髓鞘碱性蛋白(MBP)动态变化对新生儿颅内出血预后的评估价值。方法选取2019-06—2023-06亳州市人民医院收治的85例ICH新生儿为观察组,并选取同期在本院分娩的健康新生儿70例作为对照组,根据观察组预后情况分为痊愈组(n=55)和未愈组(n=30),比较各组血清S-100β、NSE、MBP水平,采用ROC曲线分析血清S-100β、NSE、MBP水平对新生儿颅内出血患儿预后不良的预测价值。结果观察组患儿出生后24 h、72 h、7 d的血清S-100β、NSE、MBP水平高于对照组(P<0.05),观察组患儿出生后72 h的血清S-100β、NSE、MBP水平最高,出生后7 d下降(P<0.05)。ICH IV级患儿出生后24 h、72 h、7 d的血清S-100β、NSE、MBP水平高于ICHⅢ级、Ⅱ级和Ⅰ级患儿,ICHⅢ级患儿出生后24 h、72 h、7 d的血清S-100β、NSE、MBP水平高于ICHⅡ级和Ⅰ级患儿,ICHⅡ级患儿出生后24 h、72 h、7 d的血清S-100β、NSE、MBP水平高于ICHⅠ级患儿(P<0.05)。未愈组患儿出生后24 h、72 h、7 d的血清S-100β、NSE、MBP水平高于痊愈组(P<0.05)。ROC曲线分析显示,出生后72 h的血清S-100β、NSE、MBP水平联合预测新生儿颅内出血患儿预后不良的敏感度为93.35%,AUC为0.922,均高于单独检测(P<0.05)。结论ICH患儿血清S-100β、NSE、MBP水平随发病时间发生显著变化,与ICH分级、预后密切相关,且联合检测对ICH患儿预后不良的预测价值较高。

细孔钻颅微创血肿清除术对高血压脑出血患者血-脑屏障指数、MBP水平的影响

目的 分析细孔钻颅微创血肿清除术对高血压脑出血(HICH)患者血-脑屏障(BBB)指数、髓鞘碱性蛋白(MBP)水平的影响。方法 回顾性分析2021年3月-2022年6月期间72例在我院收治的HICH患者病例资料,根据手术方式的不同分成传统组(n=35,行传统开颅血肿清除术)和微创组(n=37,行细孔钻颅微创血肿清除术),对比两组临床疗效、围手术期的指标、BBB指数及MBP水平,对比两组手术后并发症的情况。结果 微创组HICH患者总有效率为91.89%,明显高于传统组的74.29%(P<0.05);微创组HICH患者手术时间、术中出血量以及术后住院时间均优于传统组(P<0.05);术后1周,微创组和传统组术后BBB指数及MBP水平均较手术前下降(P<0.05),且相比传统组,微创组下降的效果更为明显(P<0.05);术后3个月,微创组HICH患者GOS评分高于传统组,微创组NIHSS评分较术前明显下降,且低于传统组(P<0.05);微创组HICH患者手术后感染、继发血肿的发生率显著低于传统组(P<0.05),两组对比血肿清除率以及发生其他并发症的情况差异不明显(P>0.05)。结论 细孔钻颅微创血肿清除术可有效降低BBB指数及MBP水平,提高HICH治疗的效果。

Effect of electro-acupuncture on basic fibroblast growth factor protein and mRNA expression in hippocampal dentate gyrus of spleen deficiency rats

BACKGROUND： Spleen deficiency in traditional Chinese medicine refers to the functional disorder of spleen, pancreas, intestines, and nervous system in modern medicine. OBJECTIVE; To test whether electro-acupuncture could alter basic fibroblast growth factor （bFGF） protein and mRNA expression in the hippocampal dentate gyrus of spleen deficiency rats. DESIGN, TIME AND SETTING： The randomized, controlled, in vivo animal experiment was performed at the National LeveI-B Laboratory of Clinical Cell Molecule and Biology in Shenzhen Hospital of Traditional Chinese Medicine, between March and November in 2008. MATERIALS： Reserpine injection was produced by Guangdong Bangmin Pharmaceutical Co. Rhubarb extract granule preparation was produced by Guangdong Yifang Pharmaceutical. Huanqiu Brand sterile acupuncture pin was provided by Suzhou Acupuncture Supplies, China. Huatuo Brand electroacupuncture instrument （type SDZ-II） was purchased from Suzhou Medical Appliance Factory, China. METHODS： A total of 96 male Sprague Dawley rats were randomly assigned to control （n = 32） and induction （n = 64） groups. Spleen deficiency was induced via intraperitoneal injection of reserpine and intragastric administration of rhubarb. The successful models were randomized into two groups： model and electro-acupuncture, with 32 rats in each group. Electro-acupuncture was administered at Zusanfi （ST 36） and Sanyinjiao （SP 6） acupoints using a condensation wave and rarefaction （condensation wave 15 Hz） at a strength of 6-15 V for 20 minutes, once per day. The appearance of a slight shiver in the corresponding locus was taken as the standard. According to electro- acupuncture time points, each group was assigned to four subgroups at 7, 14, 28, and 49 days, respectively, with eight rats in each subgroup. Immunohistochemical staining, image analysis, and reverse-transcription polymerase chain reaction were performed at different time points. MAIN OUTCOME MEASURES： bFGF protein and mRNA expression in the hippocampal dentate gyrus of spleen deficiency rats. RESULTS： After 7 days of electro-acupuncture therapy, bFGF protein and mRNA expression significantly increased compared with the model and control groups （P 〈 0.05）. After 14 days, bFGF protein and mRNA expression decreased until 28 days, where levels were then equal to the model group and greater than the control group （P 〈 0.05）. After 49 days, the above indices remained increased in the electro-acupuncture group compared to the model and control groups （P 〈 0.05）. CONCLUSION： Continuous electro-acupuncture maintained a high level of bFGF protein and mRNA expression in the hippocampal dentate gyrus of spleen deficiency rats.

Trends in global warming and evolution of polymerase basic protein 2 family from influenza a virus

Both global warming and influenza trouble humans in varying ways, therefore it is important to study the trends in both global warming and evolution of influenza A virus, in particular, proteins from influenza A virus. Recently, we have conducted two studies along this line to determine the trends between global warming and polymerase acidic protein as well as matrix protein 2. Although these two studies reveal some interesting findings, many studies are still in need because at least there are ten different proteins in influenza A virus. In this study, we analyze the trends in global warming and evolution of polymerase basic protein 2 (PB2) from influenza A virus. The PB2 evolution from 1956 to 2008 was defined using the unpredictable portion of aminoacid pair. Then the trend in this evolution was compared with the trend in the global temperature, the temperature in north and south hemispheres, and the temperature in influenza A virus sampling site and species carrying influenza A virus. The results show the similar trends in global warming and in PB2 evolution, which are in good agreement with our previous studies in polymerase acidic protein and matrix protein 2 from influenza A virus.

Acupuncture effects on serum myelin basic protein and remyelination following 30 minutes and 2 hours of ischemia in a rat model of cerebral ischemia-reperfusion injury

BACKGROUND： Acupuncture treatment on injured cerebral axons has shown to provide efficacy in clinical practice. It is unknown whether acupuncture produces therapeutic effects by protecting injured cerebral myelin in ischemic stroke. OBJECTIVE： To test whether acupuncture provides protection for injured cerebral myelin, based on quantitative data from cerebral ischemia-reperfusion rats, and to compare the effects of early and late acupuncture on serum myelin basic protein （MBP） content and remyelination of the ischemic internal capsule.DESIGN, TIME AND SETTING： A randomized, controlled experiment was performed at the Neurobiological Laboratory, Sichuan University from March 2005 to March 2006. MATERIALS： ＂Hua Tuo＂ Brand filiform needles were produced by the Medical Instrument Factory of Suzhou, China.METHODS： A total of 52 adult, healthy, male, Sprague Dawley rats were randomly assigned to four groups： control （n = 4）, model （n = 16）, early acupuncture （n = 16）, and late acupuncture （n = 16）. The focal cerebral ischemia-reperfusion model was established by middle cerebral artery occlusion in the right hemisphere using the modified thread embolism method in the latter three groups. Early and late acupuncture groups underwent acupuncture after ischemia for 30 minutes and 2 hours using the Xingnaokaiqiao needling method, respectively. Acupoints were ＂Neiguarf＇ （PC 6） and ＂Sanyinjiao＂ （SP 6） on the bilateral sides, as well as ＂Shuigou＇ （DU 26） and ＂Baihui＂ （DU 20） with stimulation for 1 minute at each acupoint. Acupuncture at all acupoints was performed two or three times while the needle was retained, once per day. No special handling was administered to the control clroup.MAIN OUTCOME MEASURES： For each group, remyelination of the internal capsule was observed by Pal-Weigert＇s myelin staining and serum MBP content was detected using enzyme-linked immunosorbent assay method on days 1,3, 5, and 7 following ischemia-reperfusion injury.RESULTS： Compared with the control group, massive demyelination of the internal capsule occurred, and serum MBP content increased in the model group （P 〈 0.05）. Compared with the model group, the extent of demyelination in the internal capsule was less distinct and serum MBP content was significantly less in the early and late acupuncture group （P 〈 0.01 ）. Compared with the late acupuncture group, serum MBP content reached a peak later and the peak value was less in the early acupuncture group. CONCLUSION： Results suggest that acupuncture exerts a protective effect on injured cerebral myelin in ischemia-reperfusion rats by reducing serum MBP content and promoting remyelination. The study also suggests that the effect of early acupuncture is superior to late acupuncture.

Neuron-specific Enclose and Myelin Basic Protein in Cerebrospinal Fluid of Patients with First Episode Schizophrenia

In order to study whether patients with schizophrenia have cerebral injury, neuron-specific enolase （NSE） and myelin basic protein （MBP）in cerebrospinal fluid （CSF） of 33 patients with first episode schizophrenia and 9 from the control group were determined by double antibody sandwich enzyme immunoassay method. The results showed that there was significant difference in the NSE contents between the experimental group and control group （P〈0.01）. The NSE contents in CSF in the experimental group were positively correlated with MBP in schizophrenia patients （P〈 0.05）. These findings suggested that patients with schizophrenia had cerebral injury.

Effects of basic fibroblast growth factor on hippocampal and parietal cortical neuronal cAMP-response element-binding protein expression in a rat model of focal cerebral ischemia/reperfusion

BACKGROUND： cAMP-response element binding protein （CREB） is a key modulator of various signaling pathways. CREB activation initiates a series of intracellular signaling pathways that promote neuronal survival. OBJECTIVE： To investigate the regulatory effects of basic fibroblast growth factor （bFGF） on cerebral neuronal CREB expression following ischemia/reperfusion injury. DESIGN, TIME AND SETTING： An immunohistochemical detection experiment was performed at the Department of Anatomy, Shenyang Medical College, between October 2006 and April 2008. MATERIALS： A total of 60 healthy, adult, Wistar rats were randomly divided into three groups： sham-operated （n =12）, ischemia/reperfusion （n = 24）, and bFGF-treated （n = 24）. Rabbit anti-rat CREB （1： 100） and biotin labeled goat anti-rabbit IgG were purchased from the Wuhan Boster Company, China. MetaMorph-evolution MP5.0-BX51 microscopy imaging system was provided by China Medical University, China. METHODS： Rat models of cerebral ischemia/reperfusion injury were developed using the suture method for right middle cerebral artery occlusion. Two-hour ischemia was followed by reperfusion. Rats from the bFGF-treated and ischemia/reperfusion groups were intraperitoneally administered endogenous bFGF （500 IU/mL, 2 000 IU/kg） or an equal amount of physiological saline. Rats from the sham-operated group underwent a similar surgical procedure, without induction of ischemia/reperfusion injury and drug administration. MAIN OUTCOME MEASURES： After 48-hour reperfusion, hippocampal and parietal cortical neuronal CREB expression was detected by immunohistochemistry, and the absorbance of hippocampal CREB-positive products was determined using MetaMorph-evolutionMP5.0-BX51 microscopy imaging system. RESULTS： The sham-operated group exhibited noticeable CREB expression in hippocampal and parietal cortical neurons. In the ischemia/reperfusion group, the CREB expression was discrete and neurons were poorly arranged. The bFGF-treated group exhibited increased CREB expression and better neuronal arrangement compared with the ischemia/reperfusion group. The mean absorbance of CREB-immunoreactive products in the hippocampus and parietal cortex was significantly higher in the ischemia/reperfusion group than in the sham-operated group （P 〈 0.05）, and significantly higher in the bFGF-treated group than in the ischemia/reperfusion group （P 〈 0.05）. CONCLUSION： bFGF significantly upregulates CREB expression in hippocampal and parietal cortical neurons following ischemia/reperfusion injury.

Analysis of the induction of the myelin basic protein binding to the plasma membrane phospholipid monolayer

Myelin basic protein（MBP） is an essential structure involved in the generation of central nervous system（CNS）myelin.Myelin shape has been described as liquid crystal structure of biological membrane.The interactions of MBP with monolayers of different lipid compositions are responsible for the multi-lamellar structure and stability of myelin.In this paper,we have designed MBP-incorporated model lipid monolayers and studied the phase behavior of MBP adsorbed on the plasma membrane at the air/water interface by thermodynamic method and atomic force microscopy（AFM）.By analyzing the pressure–area（π–A） and pressure–time（π–T） isotherms,univariate linear regression equation was obtained.In addition,the elastic modulus,surface pressure increase,maximal insertion pressure,and synergy factor of monolayers were detected.These parameters can be used to modulate the monolayers binding of protein,and the results show that MBP has the strongest affinity for 1,2-dipalmitoyl-sn-glycero-3-phosphoserine（DPPS） monolayer,followed by DPPC/DPPS mixed and1,2-dipalmitoyl-sn-glycero-3-phospho-choline（DPPC） monolayers via electrostatic and hydrophobic interactions.AFM images of DPPS and DPPC/DPPS mixed monolayers in the presence of MBP（5 n M） show a phase separation texture at the surface pressure of 20 m N/m and the incorporation of MBP put into the DPPC monolayers has exerted a significant effect on the domain structure.MBP is not an integral membrane protein but,due to its positive charge,interacts with the lipid head groups and stabilizes the membranes.The interaction between MBP and phospholipid membrane to determine the nervous system of the disease has a good biophysical significance and medical value.

Triethylamine or diethylenetriamine as dynamic modifier for suppressing basic protein adsorption in capillary electrophoresis

Either triethylamine or diethylenetriamine can be conveniently used as a dynamic modifier to suppress the adsorption of basic proteins in capillary zone electrophoresis. Sufficiently high column efficiencies (> 2x10(5) plates/m for cytochrome C) were obtained with either of them along with the improvement of the peak shapes and repeatability of migration time. The relationship between the adsorption suppression effect of the modifier and its structure was discussed.

Effects of diethyldithiocarbamate on myelin basic protein expression in the rat lateral olfactory tract

BACKGROUND： Dithiocarbamates can cause demyelination of axons in the peripheral nervous system. Its derivate, diethyldithiocarbamate, is cytotoxic, and causes olfactory mucosal damage and atrophy of the olfactory bulb. However, it is still unclear whether the myelin sheath of the lateral olfactory tract is affected by diethyldithiocarbamate. OBJECTIVE： To investigate the effects of diethyldithiocarbamate on the myelin sheath of the rat lateral olfactory tract. This was done by examining changes in myelin basic protein expression after diethyldithiocarbamate treatment. DESIGN, TIME AND SETTING： A randomized, controlled, animal study was performed at the Laboratory of the Department of Human Anatomy and Neurobiology, Xiangya School of Medicine, Central South University, China from July to November 2007. MATERIALS： A total of 72 Sprague Dawley rats were randomly assigned into a diethyldithiocarbamate group （n = 32）, a solvent control group （n = 32）, and a blank control group （n = 8）. The diethyldithiocarbamate and solvent control groups were separately divided into 3-d, 7-d, 14-d and 28-d survival subgroups, with eight rats in each. Diethyldithiocarbamate （Sigma, USA） and goat anti-myelin basic protein polyclonal antibody （Santa Cruz, USA） were used in this study. METHODS： Rats in the diethyldithiocarbamate and solvent control groups were subcutaneously injected with diethyldithiocarbamate （600 mg/kg） and 0.01 mol/L phosphate buffered saline （600 mg/kg） at the posterior neck, respectively. Rats in the blank control group received no treatment. MAIN OUTCOME MEASURES： Immunohistochemical staining and Western blot assay were used to measure myelin basic protein expression in the rat lateral olfactory tract. RESULTS： Following immunohistochemical staining, myelin basic protein was uniformly distributed in the rat lateral olfactory tract in the blank control and solvent control groups. Western blot assay showed 21.5, 18, 17 and 14 ku positive bands. No significant difference was found in myelin basic protein distribution and blot pattern, in the rat lateral olfactory tract, in the diethyldithiocarbamate group, following immunohistochemical staining and Western blot assay. Myelin basic protein expression gradually decreased at day 3, reached the lowest level at day 7, and gradually increased again at days 14 and 28. CONCLUSION： Demyelination is induced by diethyldithiocarbamate in the rat lateral olfactory tract in an early stage, followed by remyelination at later stages.

Effects of Propofol combined with remifentanil on the levels of MBP,NSE and S100B protein,D-D and inflammatory factors in patients with acute craniocerebral trauma

Objective: To investigate the effects of Propofol combined with remifentanil on serum levels of MBP, NSE and S100B protein, D-D and inflammatory factors in patients with acute craniocerebral trauma. Methods: A total of 100 patients were selected with traumatic brain injury who underwent emergency surgery from August 2014 to May 2017 in our hospital, then randomly divided them into the control group and the experimental group, 50 cases each. The control group received isoflurane combined with remifentanil to maintain anesthesia, and the experimental group received propofol and remifentanil to maintain anesthesia. The inflammatory factors and the levels of MBP, NSE, S100B and D-D in the two groups before and after anesthesia (T0), 1H (T1) and postoperative 1H (T2) were detected and compared. Results: There was no significant difference between the two groups in the levels of TNF-α. The serum level of hs-CRP in two groups of T1, T2 increased significantly, the difference was statistically significant compared with T0, in the experimental group, serum level of hs-CRP at T1 and T2 was significantly higher than the control group, the difference was statistically significant. Conclusion: Propofol combined with remifentanil anesthesia for acute craniocerebral trauma can maintain the balance of inflammatory cytokine levels during the perioperative period, inhibit the elevation of serum MBP, NSE, S100B protein and D-D levels, reduce brain cell damage. It has a good protective effect on brain cells and is worthy of clinical application.

Adaptation in polymerase basic protein 1 family from influenza A virus to climate change

Global climate changes affect the functioning of ecosystems, in particular host-pathogen interactions, with major consequences in health ecology, however, it is less addressed how the change in global temperature affects the protein family of influenza virus. In this study, we studied the adaptation of polymerase basic protein 1 (PB1) family from influenza A virus to temperature change. 3841 PB1 proteins sampled from 1956-2011 were quantified by the amino-acid pair predictability and then compared their general changes with the temperature changes (Had-CRUT3v and CRUTEM4v data sets) of corresponding years on a 5? by 5? grid-box basis. Also, point-to-point comparisons were conducted from 1956 to 1998 in all and different species. The results showed that both changes in the temperature and unpredictable portion of PB1 proteins had similar trends from 1956 to 2011, which provides the evidence of virus adaptation at protein level to climate change.

Capillary Zone Electrophoretic Separation of Basic Proteins with Coated Columns Prepared by Sol-Gel Technology

Coated capillary columns were prepared by sol-gel technology and used in the separation of basic proteins with capillary zone electrophoresis. The results indicated that a significant decrease in protein adsorption was obtained and EOF was also diminished to zero in the pH range of 3-10.

血清UA、Hcy、MBP水平对精神分裂症患者病情严重程度及认知功能障碍程度的评估价值

目的探讨血清尿酸(UA)、同型半胱氨酸(Hcy)、髓鞘碱性蛋白(MBP)水平对精神分裂症患者病情严重程度及认知功能障碍程度的评估价值。方法前瞻性选取2021年2月至2023年2月河南省荣康医院收治的104例精神分裂症患者作为研究组,同期104例健康体检者作为对照组。比较两组受检者的血清UA、Hcy、MBP水平;比较不同病情程度患者、不同认知功能患者入院时的血清水平、阳性与阴性症状量表(PANSS)评分及简易智力状态检查(MMSE)评分;采用Pearson、Spearman相关分析法分析入院时患者血清水平与精神分裂症、PANSS、MMSE评分的相关性;采用受试者工作特性曲线(ROC)分析血清UA、Hcy、MBP水平联合评估重度精神分裂症的诊断价值。结果研究组患者的血清UA、Hcy、MBP水平分别为(372.46±33.18)μmol/L、(22.38±4.19)μmol/L、(0.88±0.21)mg/L,明显高于照组的(255.31±24.08)μmol/L、(5.71±1.26)μmol/L、(0.27±0.06)mg/L,差异均有统计学意义(P<0.05);不同病情程度患者入院时的血清UA、Hcy、MBP水平及PANSS评分比较,重度>中度>轻度,差异均有统计学意义(P<0.05);不同认知功能患者入院时的血清UA、Hcy、MBP水平比较,重度认知功能障碍>中度认知功能障碍>轻度认知功能障碍,差异均有统计学意义(P<0.05);轻度认知功能障碍患者的MMSE评分为(23.52±0.98)分,明显高于中度认知功能障碍的(16.39±1.16)分和重度认知功能障碍的(5.14±1.23)分,差异均有统计学意义(P<0.05);经Pearson、Spearman分析结果显示,血清UA、Hcy、MBP水平与精神分裂症、PANSS评分均呈正相关(P<0.05),与MMSE评分均呈负相关(P<0.05);经ROC分析结果显示,血清UA、Hcy、MBP联合鉴别诊断中度精神分裂症的AUC为0.908,敏感度为82.86%,特异度为86.21%,大于单独指标鉴别诊断(P<0.05);血清UA、Hcy、MBP联合鉴别诊断重度精神分裂症的AUC为0.937,敏感度为82.50%,特异度为91.43%,大于单独指标鉴别诊断(P<0.05)。结论血清UA、Hcy、MBP水平变化可敏感反映精神分裂症患者病情严重程度及认知功能障碍程度,可作为临床诊断的重要参考依据。

三七通舒胶囊联合丁苯酞对急性脑梗死患者血液流变学、MBP、MCP-1及ET-1的影响

目的探究三七通舒胶囊联合丁苯酞对急性脑梗死患者血液流变学、髓鞘碱性蛋白(myelin basic protein,MBP)、单核细胞趋化蛋白-1(monocyte chemotactic protein-1,MCP-1)及内皮素-1(endothelin-1,ET-1)的影响。方法选取2021年1月至2022年4月于义乌市中心医院就诊的106例急性脑梗死患者,采用随机数字表法分为对照组(n=53)与观察组(n=53)。两组患者均给予常规对症支持治疗,对照组予以丁苯酞治疗,观察组在对照组基础上联合三七通舒胶囊治疗。观察两组治疗后临床疗效,比较两组治疗前后血液流变学、MBP、MCP-1、ET-1水平,以及美国国立卫生研究院卒中量表(National Institutes of Health stroke scale,NIHSS)、改良Rankin量表(modified Rankin scale,m RS)分数及Barthel指数(Barthel index,BI)。结果治疗后,观察组临床总有效率显著高于对照组(P<0.05);两组纤维蛋白原(fibrinogen,FIB)、血浆黏度(plasma viscosity,PV)、红细胞聚集指数、血细胞比容(hematocrit,HCT)、NIHSS评分、m RS评分、MBP、MCP-1及ET-1水平均较治疗前显著下降(P<0.05),而BI指数较治疗前显著升高(P<0.05)。观察组FIB、PV、红细胞聚集指数、HCT、MBP、MCP-1、ET-1水平及NIHSS评分、m RS评分显著低于对照组(P<0.05),而BI指数高于对照组(P<0.05)。结论三七通舒胶囊联合丁苯酞治疗急性脑梗死,可改善患者血液流变学、促进神经功能恢复、增强其日常生活能力,其作用机制可能与MBP、MCP-1及ET-1紊乱有关。