Strange Interlude has been considered the most complicated play ever written by Eugene O'Neill,not only because of it's great length,but also because of the complex in structure.The play conversal most the ent...Strange Interlude has been considered the most complicated play ever written by Eugene O'Neill,not only because of it's great length,but also because of the complex in structure.The play conversal most the entire life of a woman,right from her girlhood to old age.This paper intends to analyze diverse male/fem ale relationships that are evident in thisplay.展开更多
The female characters in Conrad's Heart of Darkness and Victory on one hand seem to be either too idealistic or rather uncivilized and insignificant.On the other hand,they have indispensable functions:they prepare...The female characters in Conrad's Heart of Darkness and Victory on one hand seem to be either too idealistic or rather uncivilized and insignificant.On the other hand,they have indispensable functions:they prepare the precondition for the plot,they can reverse the gender stereotype,and they help the inner growth of male protagonists.Even so,the focus of Joseph Conrad is still a patriarchal society where women are motivated by men.展开更多
AIML To investigate the effect and mechanism of action of the nitric oxide synthase (NOS) inhibitor NG-nitro-L-arginine methyl ester (L-NAME) on invasion and metastasis of human colorectal cancer cell line SL-174T...AIML To investigate the effect and mechanism of action of the nitric oxide synthase (NOS) inhibitor NG-nitro-L-arginine methyl ester (L-NAME) on invasion and metastasis of human colorectal cancer cell line SL-174T. METHODS: Human colorectal cancer cel4 line SL-174T was cultured and treated separately with four different dosages of L-NAME for 72 h, Nitric oxide (NO) production was measured with Griess reagent, The effect of L-NAME on invasion and migration of SL-174T cells were evaluated by using Transwell chambers attached with polycarbonate filters and reconstituted basement membrane (Matrigel), RT-PCR was performed to determine the mRNA levels of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor metalloproteinase-2 (TIMP-2),RESULTS: L-NAME could significantly inhibit NO production of SL-174T in a dose-dependent manner. After being treated for 72 h with 0.2, 0.4, 0.8, and 1.0 mmol/L L- NAME, respectively, the ability of the L-NAME treated SL- 174T cells to invade the reconstituted basement membrane decreased significantly (t = 8.056, P〈0.05; t= 14.467, P〈0.01; t= 27.785, P〈0.01; and t= 29.405, P〈0.01, respectively) and the inhibition rates were 10.29%, 19.62%, 34.08%, and 42.23%, respectively. Moreover, L-NAME could inhibit migration of SL-174T cells, and the inhibition rates were 20.76%, 24.95%, 39.43%, and 46. 85% for L-NAME at 0.2, 0.4, 0.8, and 1.0 mmol/L, respectively (t = 15.116, P〈0.01). In addition, after treatment with L-NAME, expression of MMP-2 mRNA was significantly decreased (t = 71.238, P〈0.01) and that of TIMP-2 mRNA was markedly increased (t = -13.020, P〈0.01). CONCLUSION: L-NAME exerts anti-invasive and anti- metastatic effects on SL-174T cell line via downregulating MNP-2 mRNA expression and upregulating TIMP-2 mRNA expression.展开更多
One of the most potent rodenticides is 2_fluoroacetamide (2_FA). Toxicity of this chemical is well documented. However, its inhalation toxicity data is not available in the literature. Hence, \{acute\} inhalation toxi...One of the most potent rodenticides is 2_fluoroacetamide (2_FA). Toxicity of this chemical is well documented. However, its inhalation toxicity data is not available in the literature. Hence, \{acute\} inhalation toxicity study was carried out by exposing male and female rats to aerosols of 2_FA at different concentrations for 4 h in a dynamically operated whole body inhalation exposure chamber. During and after the inhalation exposure the rats were less active, and showed mild tremors and convulsions. At higher concentrations the rats died after 2_3 days. The estimated 4_h LC 50 for male and female rats was 136.6 and 144.5 mg·m -3 respectively. Exposure to 0.7 LC 50 for 4 h duration showed an increase in the liver weight of male and female rats 7 days after exposure. Various haematological and biochemical variables determined were within the normal limits. However, histological findings showed injured lung as indicated by desquamation and necrosis of the epithelium of the respiratory tract. Marked hypertrophy of hepatocytes displaying strong acidophilic granulated cytoplasm was observed. Focal dilatation of renal proximal tubules in kidney with cytoplasmic vacuolation, and irregularly placed pyknotic nuclei were seen. The present study shows that 2_FA is a highly toxic chemical through the inhalation route based on the LC 50 value. Consequently necessary precautions should be taken during its handling.展开更多
文摘Strange Interlude has been considered the most complicated play ever written by Eugene O'Neill,not only because of it's great length,but also because of the complex in structure.The play conversal most the entire life of a woman,right from her girlhood to old age.This paper intends to analyze diverse male/fem ale relationships that are evident in thisplay.
文摘The female characters in Conrad's Heart of Darkness and Victory on one hand seem to be either too idealistic or rather uncivilized and insignificant.On the other hand,they have indispensable functions:they prepare the precondition for the plot,they can reverse the gender stereotype,and they help the inner growth of male protagonists.Even so,the focus of Joseph Conrad is still a patriarchal society where women are motivated by men.
文摘AIML To investigate the effect and mechanism of action of the nitric oxide synthase (NOS) inhibitor NG-nitro-L-arginine methyl ester (L-NAME) on invasion and metastasis of human colorectal cancer cell line SL-174T. METHODS: Human colorectal cancer cel4 line SL-174T was cultured and treated separately with four different dosages of L-NAME for 72 h, Nitric oxide (NO) production was measured with Griess reagent, The effect of L-NAME on invasion and migration of SL-174T cells were evaluated by using Transwell chambers attached with polycarbonate filters and reconstituted basement membrane (Matrigel), RT-PCR was performed to determine the mRNA levels of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor metalloproteinase-2 (TIMP-2),RESULTS: L-NAME could significantly inhibit NO production of SL-174T in a dose-dependent manner. After being treated for 72 h with 0.2, 0.4, 0.8, and 1.0 mmol/L L- NAME, respectively, the ability of the L-NAME treated SL- 174T cells to invade the reconstituted basement membrane decreased significantly (t = 8.056, P〈0.05; t= 14.467, P〈0.01; t= 27.785, P〈0.01; and t= 29.405, P〈0.01, respectively) and the inhibition rates were 10.29%, 19.62%, 34.08%, and 42.23%, respectively. Moreover, L-NAME could inhibit migration of SL-174T cells, and the inhibition rates were 20.76%, 24.95%, 39.43%, and 46. 85% for L-NAME at 0.2, 0.4, 0.8, and 1.0 mmol/L, respectively (t = 15.116, P〈0.01). In addition, after treatment with L-NAME, expression of MMP-2 mRNA was significantly decreased (t = 71.238, P〈0.01) and that of TIMP-2 mRNA was markedly increased (t = -13.020, P〈0.01). CONCLUSION: L-NAME exerts anti-invasive and anti- metastatic effects on SL-174T cell line via downregulating MNP-2 mRNA expression and upregulating TIMP-2 mRNA expression.
文摘One of the most potent rodenticides is 2_fluoroacetamide (2_FA). Toxicity of this chemical is well documented. However, its inhalation toxicity data is not available in the literature. Hence, \{acute\} inhalation toxicity study was carried out by exposing male and female rats to aerosols of 2_FA at different concentrations for 4 h in a dynamically operated whole body inhalation exposure chamber. During and after the inhalation exposure the rats were less active, and showed mild tremors and convulsions. At higher concentrations the rats died after 2_3 days. The estimated 4_h LC 50 for male and female rats was 136.6 and 144.5 mg·m -3 respectively. Exposure to 0.7 LC 50 for 4 h duration showed an increase in the liver weight of male and female rats 7 days after exposure. Various haematological and biochemical variables determined were within the normal limits. However, histological findings showed injured lung as indicated by desquamation and necrosis of the epithelium of the respiratory tract. Marked hypertrophy of hepatocytes displaying strong acidophilic granulated cytoplasm was observed. Focal dilatation of renal proximal tubules in kidney with cytoplasmic vacuolation, and irregularly placed pyknotic nuclei were seen. The present study shows that 2_FA is a highly toxic chemical through the inhalation route based on the LC 50 value. Consequently necessary precautions should be taken during its handling.