Male infertility is responsible for an estimated 50%of all cases of infertility.Treatments for male infertility include surgery,in-vitro fertilization,hormone therapy,and herbal remedies.Assisted reproductive technolo...Male infertility is responsible for an estimated 50%of all cases of infertility.Treatments for male infertility include surgery,in-vitro fertilization,hormone therapy,and herbal remedies.Assisted reproductive technologies and methods have made it possible to identify and treat previously untreatable causes of male infertility.Currently available treatments for male infertility are prohibitively expensive,difficult to obtain,necessitate a lengthy course of treatment,and have a host of side effects.Herbal therapy offers male infertility treatment that is less expensive and has fewer side effects than other treatments.The current review focuses on the various treatment options for male infertility.展开更多
Azoospermia patients who carry a monogenetic mutation that causes meiotic arrest may have their biological child through genetic correction in spermatogonial stem cells(SSCs).However,such therapy for infertility has n...Azoospermia patients who carry a monogenetic mutation that causes meiotic arrest may have their biological child through genetic correction in spermatogonial stem cells(SSCs).However,such therapy for infertility has not been experimentally investigated yet.In this study,a mouse model with an X-linked testis-expressed 11(TEX11)mutation(Tex11PM/Y)identified in azoospermia patients exhibited meiotic arrest due to aberrant chromosome segregation.Tex11PM/Y SSCs could be isolated and expanded in vitro normally,and the mutation was corrected by clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated endonuclease 9(Cas9),leading to the generation of repaired SSC lines.Whole-genome sequencing demonstrated that the mutation rate in repaired SSCs is comparable with that of autonomous mutation in untreated Tex11PM/Y SSCs,and no predicted off-target sites are modified.Repaired SSCs could restore spermatogenesis in infertile males and give rise to fertile offspring at a high efficiency.In summary,our study establishes a paradigm for the treatment of male azoospermia by combining in vitro expansion of SSCs and gene therapy.展开更多
Objective： To observe the clinical efficacy of acupuncture-moxibustion therapy for infertility due to sperm abnormality （SAI）. Methods： We treated a series of 35 cases of SAI with electroacupuncture combined with ...Objective： To observe the clinical efficacy of acupuncture-moxibustion therapy for infertility due to sperm abnormality （SAI）. Methods： We treated a series of 35 cases of SAI with electroacupuncture combined with herb cake-partitioned moxibustion, observing the variation before and after treatment in symptom integral, sperm status, sex hormone and prostate function. Results： After treatment, the patients were remarkably improved in symptom integral, sperm status, sex hormone and prostate function as compared with those prior to treatment. Conclusion： Acupuncture-moxibustion is an effective therapy for SAI.展开更多
Objective： Working on seminal plasma acid phosphatase, to explore the mechanism by which acupuncture improves sperm quality （concentration, viability and motility） in infertility patients. Methods： A total of 118 ...Objective： Working on seminal plasma acid phosphatase, to explore the mechanism by which acupuncture improves sperm quality （concentration, viability and motility） in infertility patients. Methods： A total of 118 patients received acupuncture-moxibustion treatment. Before and 3, and 6 months after the treatment were detected their seminal plasma acid phosphatase, sperm concentration, sperm viability and sperm motility were measured. Results： The differences between before and after treatments in sperm motility and seminal plasma acid phosphatase levels were statistically significant （P〈0.01）. Conclusion： Acupuncture-moxibustion can improve seminal plasma acid phosphatase levels in infertility patients.展开更多
基金We are grateful for the Pharmacy Department at Pranveer Singh Institute of Technology that helped us in writing this review article.
文摘Male infertility is responsible for an estimated 50%of all cases of infertility.Treatments for male infertility include surgery,in-vitro fertilization,hormone therapy,and herbal remedies.Assisted reproductive technologies and methods have made it possible to identify and treat previously untreatable causes of male infertility.Currently available treatments for male infertility are prohibitively expensive,difficult to obtain,necessitate a lengthy course of treatment,and have a host of side effects.Herbal therapy offers male infertility treatment that is less expensive and has fewer side effects than other treatments.The current review focuses on the various treatment options for male infertility.
基金This study was supported by Genome Tagging Project and grants from the Chinese Academy of Sciences,the National Key Research and Development Program of China,Shanghai Municipal Commission for Science and Technology,and the National Natural Science Foundation of China(XDB19010204,2019YFA0109900,OYZDJ-SSW-SMC023Facility-based Open Research Program,19411951800,17JC1420102,31821004,32030029,31730062,31530048,and 81672117)The research is partly supported by the Fountain-Valley Life Sciences Fund of University of Chinese Academy of Sciences Education Foundation。
文摘Azoospermia patients who carry a monogenetic mutation that causes meiotic arrest may have their biological child through genetic correction in spermatogonial stem cells(SSCs).However,such therapy for infertility has not been experimentally investigated yet.In this study,a mouse model with an X-linked testis-expressed 11(TEX11)mutation(Tex11PM/Y)identified in azoospermia patients exhibited meiotic arrest due to aberrant chromosome segregation.Tex11PM/Y SSCs could be isolated and expanded in vitro normally,and the mutation was corrected by clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated endonuclease 9(Cas9),leading to the generation of repaired SSC lines.Whole-genome sequencing demonstrated that the mutation rate in repaired SSCs is comparable with that of autonomous mutation in untreated Tex11PM/Y SSCs,and no predicted off-target sites are modified.Repaired SSCs could restore spermatogenesis in infertile males and give rise to fertile offspring at a high efficiency.In summary,our study establishes a paradigm for the treatment of male azoospermia by combining in vitro expansion of SSCs and gene therapy.
文摘Objective： To observe the clinical efficacy of acupuncture-moxibustion therapy for infertility due to sperm abnormality （SAI）. Methods： We treated a series of 35 cases of SAI with electroacupuncture combined with herb cake-partitioned moxibustion, observing the variation before and after treatment in symptom integral, sperm status, sex hormone and prostate function. Results： After treatment, the patients were remarkably improved in symptom integral, sperm status, sex hormone and prostate function as compared with those prior to treatment. Conclusion： Acupuncture-moxibustion is an effective therapy for SAI.
文摘Objective： Working on seminal plasma acid phosphatase, to explore the mechanism by which acupuncture improves sperm quality （concentration, viability and motility） in infertility patients. Methods： A total of 118 patients received acupuncture-moxibustion treatment. Before and 3, and 6 months after the treatment were detected their seminal plasma acid phosphatase, sperm concentration, sperm viability and sperm motility were measured. Results： The differences between before and after treatments in sperm motility and seminal plasma acid phosphatase levels were statistically significant （P〈0.01）. Conclusion： Acupuncture-moxibustion can improve seminal plasma acid phosphatase levels in infertility patients.
