Heterointerface Engineering-Induced Oxygen Defects for the Manganese Dissolution Inhibition in Aqueous Zinc Ion Batteries

Manganese-based material is a prospective cathode material for aqueous zinc ion batteries(ZIBs)by virtue of its high theoretical capacity,high operating voltage,and low price.However,the manganese dissolution during the electrochemical reaction causes its electrochemical cycling stability to be undesirable.In this work,heterointerface engineering-induced oxygen defects are introduced into heterostructure MnO_(2)(δa-MnO_(2))by in situ electrochemical activation to inhibit manganese dissolution for aqueous zinc ion batteries.Meanwhile,the heterointerface between the disordered amorphous and the crystalline MnO_(2)ofδa-MnO_(2)is decisive for the formation of oxygen defects.And the experimental results indicate that the manganese dissolution ofδa-MnO_(2)is considerably inhibited during the charge/discharge cycle.Theoretical analysis indicates that the oxygen defect regulates the electronic and band structure and the Mn-O bonding state of the electrode material,thereby promoting electron transport kinetics as well as inhibiting Mn dissolution.Consequently,the capacity ofδa-MnO_(2)does not degrade after 100 cycles at a current density of 0.5 Ag^(-1)and also 91%capacity retention after 500cycles at 1 Ag^(-1).This study provides a promising insight into the development of high-performance manganese-based cathode materials through a facile and low-cost strategy.

Facile synthesis of Cu-doped manganese oxide octahedral molecular sieve for the efficient degradation of sulfamethoxazole via peroxymonosulfate activation

Advanced processes for peroxymonosulfate(PMS)-based oxidation are efficient in eliminating toxic and refractory organic pol-lutants from sewage.The activation of electron-withdrawing HSO_(5)^(-)releases reactive species,including sulfate radical(·SO_(4)^(-)),hydroxyl radical(·OH),superoxide radical(·O_(2)^(-)),and singlet oxygen(1O_(2)),which can induce the degradation of organic contaminants.In this work,we synthesized a variety of M-OMS-2 nanorods(M=Co,Ni,Cu,Fe)by doping Co^(2+),Ni^(2+),Cu^(2+),or Fe^(3+)into manganese oxide oc-tahedral molecular sieve(OMS-2)to efficiently remove sulfamethoxazole(SMX)via PMS activation.The catalytic performance of M-OMS-2 in SMX elimination via PMS activation was assessed.The nanorods obtained in decreasing order of SMX removal rate were Cu-OMS-2(96.40%),Co-OMS-2(88.00%),Ni-OMS-2(87.20%),Fe-OMS-2(35.00%),and OMS-2(33.50%).Then,the kinetics and struc-ture-activity relationship of the M-OMS-2 nanorods during the elimination of SMX were investigated.The feasible mechanism underly-ing SMX degradation by the Cu-OMS-2/PMS system was further investigated with a quenching experiment,high-resolution mass spec-troscopy,and electron paramagnetic resonance.Results showed that SMX degradation efficiency was enhanced in seawater and tap water,demonstrating the potential application of Cu-OMS-2/PMS system in sewage treatment.

Synergism of preintercalated manganese ions and lattice water in vanadium oxide cathodes for high-capacity and long-life Zn-ion batteries

Aqueous Zn-ion batteries(AZIBs)are recognized as a promising energy storage system with intrinsic safety and low cost,but its applications still rely on the design of high-capacity and stable-cycling cathode materials.In this work,we present an intercalation mechanism-based cathode materials for AZIB,i.e.the vanadium oxide with pre-intercalated manganese ions and lattice water(noted as MVOH).The synergistic effect between Mn^(2+)and lattice H_(2)O not only expands the interlayer spacing,but also significantly enhances the structural stability.Systematic in-situ and ex-situ characterizations clarify the Zn^(2+)/H^(+)co–(de)intercalation mechanism of MVOH in aqueous electrolyte.The demonstrated remarkable structure stability,excellent kinetic behaviors and ion-storage mechanism together enable the MVOH to demonstrate satisfactory specific capacity of 450 mA h g^(−1)at 0.2 A g^(−1),excellent rate performance of 288.8 mA h g^(−1)at 10 A g^(−1)and long cycle life over 20,000 cycles at 5 A g^(−1).This work provides a practical cathode material,and contributes to the understanding of the ion-intercalation mechanism and structural evolution of the vanadium-based cathode for AZIBs.

Preparation of Manganese Oxide and Its Adsorption Properties

The in-situ oxidation of manganese sulfate solution with H2O_(2),sodium hypochlorite,potassium permanganate and oxygen as oxidants was investigated by means of SEM,EDS,XRD,BET and infrared analysis,and the effects of different oxidants on the morphology,phase composition,surface properties and specific surface area of manganese oxides were investigated.The experimental results show that the diameter of manganese oxide particles prepared with H_(2)O_(2)is the smallest,about 50 nm,and the specific surface area is the largest,63.8764 m^(2)/g.It has the advantages of abundant surface hydroxyl groups,no introduction of other impurities and large adsorption potential.It is most suitable to be used as an oxidant for oxidizing manganese sulfate solution to prepare manganese oxide by in-situ oxidation.Nano manganese oxide prepard by H_(2)O_(2)in-situ oxidation method is used as adsorbent to adsorb cobalt and nickel impurities in manganese sulfate.When the reaction pH is 6,the reaction time is 30min and the amount of adsorbent is 1.0 g,the adsorption rates of cobalt and nickel impurities in 100ml manganese sulfate solution are 97.59%and 97.67%,respectively.The residual amounts of cobalt and nickel meet the industrial process standard of first-class products(Co,Ni w/%≤0.005)of high-purity manganese sulfate(Hg/t4823-2015)for batteries.The study plays a guiding role in the preparation and regulation of manganese oxide,and provides a new method with high efficiency,purity and adsorbent availability for the preparation of high-purity manganese sulfate solution.

Enhanced recovery of high-purity Fe powder from iron-rich electrolytic manganese residue by slurry electrolysis

Iron-rich electrolytic manganese residue(IREMR)is an industrial waste produced during the processing of electrolytic metal manganese,and it contains certain amounts of Fe and Mn resources and other heavy metals.In this study,the slurry electrolysis technique was used to recover high-purity Fe powder from IREMR.The effects of IREMR and H2SO4 mass ratio,current density,reaction temper-ature,and electrolytic time on the leaching and current efficiencies of Fe were studied.According to the results,high-purity Fe powder can be recovered from the cathode plate,and the slurry electrolyte can be recycled.The leaching efficiency,current efficiency,and purity of Fe reached 92.58%,80.65%,and 98.72wt%,respectively,at a 1:2.5 mass ratio of H2SO4 and IREMR,reaction temperature of 60℃,electric current density of 30 mA/cm^(2),and reaction time of 8 h.In addition,vibrating sample magnetometer(VSM)analysis showed that the coercivity of electrolytic iron powder was 54.5 A/m,which reached the advanced magnetic grade of electrical pure-iron powder(DT4A coercivity standard).The slurry electrolytic method provides fundamental support for the industrial application of Fe resource recovery in IRMER.

The Influence of CO_(2) Cured Manganese Slag on the Performance and Mechanical Properties of Ultra-High Performance Concrete

The presence of toxic elements in manganese slag(MSG)poses a threat to the environment due to potential pollution.Utilizing CO_(2) curing on MS offers a promising approach to immobilize toxic substances within this material,thereby mitigating their release into the natural surroundings.This study investigates the impact of CO_(2) cured MS on various rheological parameters,including slump flow,plastic viscosity(η),and yield shear stress(τ).Additionally,it assesses flexural and compressive strengths(f_(t) and f_(cu)),drying shrinkage rates(DSR),durability indicators(chloride ion migration coefficient(CMC),carbonization depth(CD)),and the leaching behavior of heavy metal elements.Microscopic examination via scanning electron microscopy(SEM)is employed to elucidate the underlying mechanisms.The results indicate that CO_(2) curing significantly enhances the slump flow of ultra-high performance concrete(UHPC)by up to 51.2%.Moreover,it reduces UHPC’sηandτby rates ranging from 0%to 52.7%and 0%to 40.2%,respectively.The DSR exhibits a linear increase corresponding to the mass ratio of CO_(2) cured MS.Furthermore,CO_(2) curing enhances both f_(t) and f_(cu) of UHPC by up to 28.7%and 17.6%,respectively.The electrical resistance is also improved,showing an increase of up to 53.7%.The relationship between mechanical strengths and electrical resistance follows a cubic relationship.The CO_(2) cured MS demonstrates a notable decrease in the CMC and CD by rates ranging from 0%to 52.6%and 0%to 26.1%,respectively.The reductions of leached chromium(Cr)and manganese(Mn)are up to 576.3%and 1312.7%,respectively.Overall,CO_(2) curing also enhances the compactness of UHPC,thereby demonstrating its potential to improve both mechanical and durability properties.

Covalency competition induced selective bond breakage and surface reconstruction in manganese cobaltite towards enhanced electrochemical charge storage

Manganese cobaltite(MnCo_(2)_(4))is a promising electrode material because of its attractive redox chemistry and excellent charge storage capability.Our previous work demonstrated that the octahedrally-coordinated Mn are prone to react with the hydroxyl ions in alkaline electrolyte upon electrochemical cycling and separates on the surface of spinel to reconstruct into d-MnO_(2) nanosheets irreversibly,thus results in a change of the reaction mechanism with Kþion intercalation.However,the low capacity has greatly limited its practical application.Herein,we found that the tetrahedrally-coordinated Co_(2) þions were leached when MnCo_(2)_(4) was equilibrated in 1 mol L^(-1) HCl solution,leading to the formation of layered CoOOH on MnCo_(2)_(4) surface which is originated from the covalency competition induced selective breakage of the CoT–O bond in CoT–O–CoO and subsequent rearrangement of free Co_(6) octahedra.The as-formed CoOOH is stable upon cycling in alkaline electrolyte,exhibits conversion reaction mechanism with facile proton diffusion and is free of massive structural evolution,thus enables utilization of the bulk electrode material and realizes enhanced specific capacity as well as facilitated charge transfer and ion diffusion.In general,our work not only offers a feasible approach to deliberate modification of MnCo_(2)_(4)'s surface structure,but also provides an in-depth understanding of its charge storage mechanism,which enables rational design of the spinel oxides with promising charge storage properties.

CroweⅢ-Ⅳ型DDH患者全髋关节置换术后不满意及相关因素分析

目的:探讨全髋关节置换术的CroweⅢ-Ⅳ型发育性髋关节发育不良(developmental dysplasia of the hip,DDH)患者的满意度及造成不满意的相关因素。方法:回顾性分析2013年3月至2018年3月行全髋关节置换术的169例CroweⅢ-Ⅳ型DDH患者,通过微信进行调查问卷,调查患者对手术总体满意度、10项日常功能满意度和患者认为对自己日常生活影响比较大的前5个问题。手术前后采用髋关节Harris评分进行功能评价。结果:收到完整调查问卷145份,所有患者获随访,时间1~5(3.23±1.22)年。145例患者分成两组,其中对手术疗效满意的118例,不满意的27例,手术总体满意率81.38%(118/145)。患者认为对生活影响比较大的前5个问题分别是术后髋部疼痛,肢体明显不等长、行走、上下楼梯、蹲起。两组术前Harris评分比较,差异无统计学意义(P>0.05),不满意组术后Harris评分较低。术后髋关节疼痛、肢体不等长是影响手术不满意的直接因素。结论:采用全髋关节置换术治疗CroweⅢ-Ⅳ型DDH患者手术难度大;术后髋关节疼痛(轻度以上),肢体不等长(>2 cm)是术后不满意的独立危险因素。

辽宁第Ⅳ金刚石成矿带东部潘家沟辉绿岩中铬铁矿的矿物化学特征及其意义

辽宁省永宁地区因发现原生金刚石而被划分为辽宁第Ⅳ金刚石成矿带,但尚未厘定出金刚石的来源,有必要加强对与金刚石伴生的指示矿物的研究。对该成矿带东部潘家沟地区辉绿岩中选获的铬铁矿开展电子探针成分分析,并与辽宁典型金伯利岩管中的铬铁矿成分进行对比,以探讨该地区铬铁矿的成因及其与金刚石的关系。根据铬铁矿样品电子探针背散射图像显示的粒径、形态及熔蚀特征,以及铬铁矿电子探针成分测试显示的高Cr、富Mg、低Al及贫Ti的特征,推测该地区的铬铁矿来自地幔捕掳晶,属于镁铬铁矿亚类,主要为镁铁-铬铝铁亚种和镁铁-铬铝亚种。计算得到铬铁矿的形成温度为1 252~1 307℃,与前人报道的瓦房店典型金伯利岩中铬铁矿的形成温度基本一致,也接近金刚石的形成温度,表明潘家沟地区辉绿岩中铬铁矿的成因与金刚石关系密切,该地区具有良好的金刚石成矿潜力和找矿前景。研究成果对潘家沟地区金刚石原生矿的勘查及探明该地区金刚石的来源具有指导意义。

腹腔镜下侧腹壁悬吊术与阴道后壁修补术联合治疗POP-QⅢ或Ⅳ期盆腔器官脱垂的效果分析

目的:探究腹腔镜下侧腹壁悬吊术联合阴道后壁修补术在盆腔器官脱垂定量分期法(POP-Q)Ⅲ或Ⅳ期盆腔器官脱垂患者中的应用效果。方法:选择2021年2月—2023年6月厦门市第三医院收治的90例POP-QⅢ或Ⅳ期盆腔器官脱垂患者,根据随机数字表法分为对照组和观察组,各45例。对照组进行阴式子宫切除术联合阴道前后壁修补术治疗,观察组则进行腹腔镜下侧腹壁悬吊术联合阴道后壁修补术治疗。比较两组的治疗总有效率、手术指标(手术时间、术中出血量、排气时间、住院时间)、并发症发生率、手术前后的盆底相关参数[阴道总长度(TVL)、阴道前壁中线离处女膜缘3 cm处(Aa)、前穹隆或阴道顶端到Aa点之间阴道前壁上段中距离处女膜的最远点(Ba)及宫颈或子宫切除后阴道顶端所处的最远端(C)]及盆底功能障碍问卷(PFDI-20)评分。结果:观察组治疗总有效率高于对照组,手术指标均优于对照组,并发症发生率显著低于对照组,差异均有统计学意义(P<0.05)。术后6个月,观察组盆底相关参数及PFDI-20各项评分均显著低于对照组,差异均有统计学意义(P<0.05)。结论:腹腔镜下侧腹壁悬吊术联合阴道后壁修补术在POP-QⅢ或Ⅳ期盆腔器官脱垂患者中的应用效果更好,可显著改善患者的盆底相关参数及盆底功能,且安全性较高。

二肽基肽酶-Ⅳ有机小分子荧光探针的研究进展

二肽基肽酶-IV(DPP-IV)是一种重要的跨膜糖蛋白水解酶,与各种生理过程和疾病密切相关,检测DPP-IV的有机小分子荧光探针被相继报道,是当前研究生物酶检测的热点。首次综述了用于DPP-IV体外和体内检测及成像的3类有机小分子荧光探针,分别是非近红外荧光探针(λ_(em)<650nm)、近红外荧光探针(λ_(em)>650nm)和双光子荧光探针,对比并总结了三种探针的设计原理、结构特点、生物应用情况及其在DPP-IV相关疾病诊断中的应用,并探讨了DPP-IV活性检测在未来发展方向上所面临的挑战。

热淋清颗粒治疗尿路感染的多中心、开放性、Ⅳ期临床研究

目的评价热淋清颗粒在临床使用中的安全性与有效性,以指导优化临床给药方案和提高合理用药水平。方法在51家医院进行开放性、Ⅳ期临床试验,受试者为下焦湿热型单纯性或复杂性尿路感染,单纯性尿路感染患者给予热淋清颗粒每次8 g、每天3次,疗程5 d。复杂性尿路感染患者给予热淋清颗粒每次8 g、每天3次,同时联合喹诺酮类抗生素,疗程7~14 d。结局指标为临床疗效、中医证候疗效、细菌清除率以及安全性。结果共纳入2009例受试者,其中11患者失访脱落。全分析集纳入1998例,其中单纯性尿路感染患者1532例,复杂性尿路感染患者466例。临床治愈率为68.57%,单纯性和复杂性尿路感染患者分别为74.41%和49.36%。中医证候痊显率为72.22%,单纯性和复杂性尿路感染患者分别为75.13%和62.66%。单纯性尿路感染中细菌培养阳性者的细菌转阴率为57.75%,细菌清除率为58.02%;复杂性尿路感染中细菌培养阳性者的细菌转阴率为45.59%,细菌清除率为43.92%。药物相关的不良反应发生24例次,其中单纯性尿路感染17例次,复杂性尿路感染7例次。结论热淋清颗粒治疗单纯性尿路感染的有效性和安全性较好,也可改善下焦湿热证中医证候。

经食管超声心动图在肾切除术联合MayoⅢ~Ⅳ级静脉瘤栓取栓术不同手术方式中的临床作用

目的:比较经食管超声心动图(transesophageal echocardiography,TEE)在肾切除术联合下腔静脉MayoⅢ~Ⅳ级瘤栓取栓术不同手术方式中的临床作用。方法:纳入2022年1月至2024年2月在北京大学第三医院行根治性肾切除联合MayoⅢ~Ⅳ级瘤栓取栓手术的患者28例,其中,机器人手术16例,腹腔镜手术2例,开腹手术10例,收集患者的各项临床资料进行分析比较。结果:机器人手术的患者中有9例采用TEE,其中7例术中TEE影像结果较术前发生变化,包括2例术中TEE提示瘤栓进入右心房,2例显示下腔静脉瘤栓由MayoⅢ级升至Ⅳ级,3例提示瘤栓与下腔静脉粘连,及时调整了手术方案;开腹手术的患者中有6例采用TEE,其中4例术中TEE提示Mayo分级较术前发生变化,包括3例提示瘤栓与下腔静脉粘连,1例提示瘤栓伴血栓形成,调整了手术方案,旷置或节段性切除瘤栓;腹腔镜手术的2例患者未采用术中TEE。术中采用TEE的作用包括开腹手术术中探查结合TEE监测瘤栓切除过程,机器人手术完全通过TEE监测瘤栓脱出,术中TEE还实时监测患者循环状态和心脏功能变化。结论:肾切除术联合MayoⅢ~Ⅳ级瘤栓取栓术的不同术式中,术中TEE均可再次确定瘤栓分级、粘连程度,并实时动态跟踪取栓过程,监测患者循环状态和心脏功能变化,具有重要的辅助作用,但其临床应用仍不足,建议这类手术均采用术中TEE。

张守琳运用“温阳解毒通络”法治疗糖尿病肾脏病(Ⅳ-Ⅴ期)经验探析

糖尿病肾脏病属中医学的“肾消(消肾)”“下消”“水肿”等范畴,目前已成为终末期肾病的主要原因。张守琳教授认为“阳虚毒瘀肾络”是糖尿病肾脏病(Ⅳ-Ⅴ期)的基本病机,阳气受损为发病之本,毒瘀肾络是发病的关键环节,并确立了“温阳解毒通络”的治疗原则,有效延缓了肾功能损伤的进展和进入透析的进程。

纳武利尤单抗联合安罗替尼对Ⅲb~Ⅳ期NSCLC的疗效观察及对miR-1269、miR-204-5p表达的影响

目的探究纳武利尤单抗联合安罗替尼治疗Ⅲb~Ⅳ期非小细胞肺癌(NSCLC)患者的效果及对微小RNA-1269(miR-1269)、miR-204-5p表达的影响。方法选取Ⅲb~Ⅳ期NSCLC患者82例,患者分配为对照组、观察组,各41例,使用随机数字表法,对照组采用安罗替尼治疗,观察组在对照组的基础上采用纳武利尤单抗治疗;比较两组患者的疗效、治疗前后肿瘤标志物癌胚抗原(CEA)、细胞角蛋白19片段抗原21-1(CYFRA21-1)、miR-1269、miR-204-5p表达水平和不良反应的发生率。结果观察组患者治疗后疾病控制率(87.80%,36/41)显著高于对照组(68.29%,28/41)(χ^(2)=4.556,P=0.033);与治疗前相比,治疗后观察组和对照组的CEA、CYFRA21-1、miR-1269水平显著降低,miR-204-5p水平显著升高(P<0.05);治疗后与对照组相比,观察组的CEA、CYFRA21-1、miR-1269水平显著降低,miR-204-5p水平显著升高(P<0.05);不良反应两组间比较,差异无统计学意义(P>0.05)。结论纳武利尤单抗联合安罗替尼治疗Ⅲb~Ⅳ期NSCLC患者可降低患者肿瘤标志物的水平,调节miR-1269、miR-204-5p的表达,效果更好。

P92钢接头Ⅳ型开裂蠕变孔洞临界值的分析及应用

【目的】分析P92管道接头热影响区发生Ⅳ型开裂的蠕变孔洞临界值的影响因素,得到蠕变孔洞临界值计算方法,为评估接头开裂风险提供依据。【方法】收集服役后发生Ⅳ型开裂的P92接头,在未开裂处取样进行610℃下不同应力条件的高温持久蠕变试验,然后使用光学显微镜、电子显微镜观察服役开裂接头和持久断裂接头热影响区的蠕变孔洞分布形貌,并借助图像分析软件测量蠕变孔洞的面积分数,同时使用硬度仪测试热影响区软化情况,最后综合测试结果分析细晶区软化程度和接头应力水平对蠕变孔洞临界值的影响,并拟合应力、显微硬度、孔洞临界值三者之间的函数关系。【结果】研究发现,P92钢接头细晶区相比于接头其它区域软化速度更快,但软化作用并非其发生Ⅳ型开裂的必要条件,当应力水平较高时,细晶区没有发生明显软化时就可以触发蠕变开裂;得到接头轴向应力水平、蠕变孔洞面积分数临界值和显微硬度的函数关系式。【结论】引起Ⅳ型开裂的蠕变孔洞面积分数临界值与相对应力水平(轴向应力与硬度的比值)有关,相对应力水平越高,蠕变孔洞临界值越小,接头安全性就越低;通过测量接头细晶热影响区的蠕变孔洞面积分数和显微硬度,可以估算开裂接头的轴向应力水平,也可以评估在役未开裂接头的Ⅳ型开裂风险。

基于MIMIC-Ⅳ数据库的脓毒性心肌病风险预测模型构建与评价

目的分析脓毒性心肌病(SC)的危险因素,构建与评价SC风险预测模型。方法从MIMIC-Ⅳ数据库中提取脓毒症患者的临床数据,按7∶3比例随机分为训练集和验证集。根据是否患有SC,分为SC组和非SC组。通过单因素和多因素Logistic回归分析筛选SC的独立危险因素,构建风险预测模型,并绘制列线图。采用受试者工作特征曲线下面积(AUC)评估模型的区分度,校正曲线评估模型的校准度,决策曲线分析(DCA)评估模型的临床适用度。结果共纳入2628例脓毒症患者,其中训练集1865例,验证集763例。训练集和验证集SC发病率差异无统计学意义(58.98%比62.25%,P=0.120)。除慢性阻塞性肺疾病(P=0.015)和重症监护室住院时长(P=0.016),其余临床指标两组间差异均无统计学意义(P均>0.05)。Logistic回归分析结果显示,既往患有冠心病(P=0.028)、心力衰竭(P<0.001)、中性粒细胞计数升高(P=0.001)、淋巴细胞计数降低(P=0.036)、肌酸激酶同工酶升高(P<0.001)、尿素氮升高(P=0.042)是SC的独立危险因素。训练集列线图预测模型的AUC为0.759(95%CI=0.732~0.785),验证集列线图预测模型的AUC为0.765(95%CI=0.723~0.807);两个数据集拟合度较好(训练集P=0.075,验证集P=0.067);DCA结果显示列线图预测模型具有良好的临床适用度。结论基于基础疾病及临床生化指标构建的列线图预测模型能够较好地预测SC的发生风险。

1例糖原累积病Ⅳ型合并急性肝衰竭患儿急性期的护理

总结2022年7月我科收治的1例糖原累积病Ⅳ型(glycogen storage disease typeⅣ,GSDⅣ)合并急性肝衰竭患儿急性期的护理经验。护理要点:监测血流动力学,积极抗休克;监测消化道出血量,实施止血治疗;开展人工肝治疗及预防相关并发症;连续监测血糖,根据病情与血糖动态调整营养治疗方案;开展远程探视和音乐治疗。经过6 d的救治和护理,患儿病情好转,顺利进行肝移植。术后18 d好转出院;随访1年,恢复良好。

瞬时感受器电位离子通道香草素受体亚家族Ⅳ在帕金森病细胞模型中介导PC12细胞炎症反应的机制

目的探讨瞬时感受器电位离子通道香草素受体亚家族Ⅳ(TRPV4)在帕金森病(PD)细胞模型中介导PC12细胞炎症反应的机制。方法2023年6―8月,用1-甲基-4-苯基吡啶离子(MPP^(+))建立PD细胞模型,用TRPV4特异性抑制剂HC067047抑制TRPV4。将培养的PC12细胞采用随机数字表法分为四组:对照组、HC067047组、MPP^(+)组、HC067047+MPP^(+)组。细胞毒性检测试剂盒(CCK-8)检测各组细胞的增殖活力。蛋白印迹法和酶联免疫吸附测定(ELISA)检测各组细胞TRPV4和炎症因子白细胞介素-18(IL-18)、白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)的水平变化。结果与对照组1.00±0.08相比,MPP^(+)组TRPV4的表达量2.14±0.20明显升高(P<0.001)。与对照组1.00±0.01相比,MPP^(+)组的PC12细胞活力0.65±0.08明显降低(P<0.01),而HC067047能明显回复MPP^(+)引起的细胞活力0.83±0.07降低(P<0.01)。与对照组相比,MPP^(+)组的IL-181.96±0.27和IL-61.92±0.18、IL-1β(874.61±108.09)ng/L和TNF-α(791.28±106.88)ng/L明显升高(P<0.001),HC067047能明显抑制MPP^(+)引起的IL-181.45±0.11和IL-61.58±0.22、IL-1β(626.28±84.53)ng/L和TNF-α(592.94±86.9)4 ng/L明显升高(P<0.01或P<0.05)。结论TRPV4参与了MPP^(+)诱导的PD细胞模型的炎症反应,抑制TRPV4有抗炎作用。

皮肤石蜡组织Ⅳ型胶原免疫荧光抗原修复方法的评估

目的 寻找皮肤石蜡包埋组织Ⅳ型胶原免疫荧光(IF-PE)最佳抗原修复方法。方法以2021年1月至2022年12月在上海交通大学医学院附属儿童医院确诊的X-连锁Alport综合征患儿和父(母)亲共14例皮肤组织,冰冻组织免疫荧光(IF-FT)为自身对照,比较不同抗原修复法IF-PE染色部位和强度。结果 与Ⅳ型胶原IF-FT自身对照比较,采用蛋白酶K修复,仅部分患儿基膜正常分布(6/14),而角质层均异常表达;微波、高温水浴和高压蒸汽3种修复方法,基膜均无正常分布,角质层异常分布(分别为2/14,11/14,12/14);微波联合酶修复法仅2例基膜正常分布,未见角质层异常表达;高压蒸汽联合酶和高温水浴联合酶2种修复法基膜全部正常分布,角质层异常表达(分别为14/14和2/14),表达强度两者接近。结论高温水浴联合蛋白酶K可作为皮肤石蜡包埋组织Ⅳ型胶原免疫荧光理想的抗原修复法。