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拟南芥微管结合蛋白MAP70-4的生物信息学分析
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作者 吕毅 商澎 《科技通报》 2019年第8期59-63,共5页
微管结合蛋白对微管蛋白的结构及功能至关重要。为能较全面地揭示拟南芥微管结合蛋白MAP70-4的结构和功能,利用生物信息学方法对该蛋白的理化性质、亲水/疏水性,信号肽,跨膜区域,磷酸化位点,卷曲螺旋结构,蛋白质二级、三级结构,亚细胞定... 微管结合蛋白对微管蛋白的结构及功能至关重要。为能较全面地揭示拟南芥微管结合蛋白MAP70-4的结构和功能,利用生物信息学方法对该蛋白的理化性质、亲水/疏水性,信号肽,跨膜区域,磷酸化位点,卷曲螺旋结构,蛋白质二级、三级结构,亚细胞定位,表达模式,分子功能及其参与的生物学功能,以及与其它蛋白的相互作用关系进行了预测分析。结果显示,拟南芥MAP70-4蛋白是一种无信号肽,不含跨膜螺旋区域的不稳定蛋白质,属于碱性、亲水性蛋白质,翻译后会产生磷酸化修饰,该蛋白二级结构含有11个α螺旋区和5个β折叠,以及7个卷曲螺旋结构。亚细胞定位显示该蛋白主要分布在细胞质中,具有促进微管结合等功能,和其有相互作用关系的蛋白可能与叶绿体生命活动及胚胎、种子发育有关。从表达模式上看,该蛋白主要在花、果实、叶片中大量表达,根中有少量表达。通过对拟南芥微管结合蛋白MAP70-4进行系统性预测分析,可为下一步对该蛋白功能的深入研究提供理论基础和借鉴。 展开更多
关键词 拟南芥 微管结合蛋白 map70-4 生物信息学 蛋白质结构和功能预测
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利用不依赖连接反应的克隆方法构建pCHF3-MAP65-1-YFP和pCHF3-MAP65-2-YFP表达载体 被引量:1
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作者 王培培 韩榕 《江苏农业学报》 CSCD 北大核心 2018年第2期266-270,共5页
利用不依赖于连接反应的克隆(Ligation-independent cloning,LIC)方法构建了p CHF3-MAP65-1-YFP和p CHF3-MAP65-2-YFP载体,用于研究拟南芥微管结合蛋白MAP65-1和MAP65-2在细胞中的定位及UV-B辐射下有丝分裂异常现象的产生机制。试验结... 利用不依赖于连接反应的克隆(Ligation-independent cloning,LIC)方法构建了p CHF3-MAP65-1-YFP和p CHF3-MAP65-2-YFP载体,用于研究拟南芥微管结合蛋白MAP65-1和MAP65-2在细胞中的定位及UV-B辐射下有丝分裂异常现象的产生机制。试验结果表明,相对于传统载体构建方法,LIC方法试验设计操作简便,耗时短,转化率高,可减少后期重组子筛选工作。 展开更多
关键词 LIC方法 拟南芥 map65-1 map65-2
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HYPERSTATIC STRUCTURE MAPPING MODEL BUILDING AND OPTIMIZING DESIGN 被引量:2
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作者 XU Gening GAO Youshan +1 位作者 ZHANG Xueliang YANG Ruigang 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2007年第1期55-59,共5页
Hyperstatic structure plane model being built by structural mechanics is studied. Space model precisely reflected in real stress of the structure is built by finite element method (FEM) analysis commerce software. M... Hyperstatic structure plane model being built by structural mechanics is studied. Space model precisely reflected in real stress of the structure is built by finite element method (FEM) analysis commerce software. Mapping model of complex structure system is set up, with convenient calculation just as in plane model and comprehensive information as in space model. Plane model and space model are calculated under the same working condition. Plane model modular construction inner force is considered as input data; Space model modular construction inner force is considered as output data. Thus specimen is built on input data and output dam. Character and affiliation are extracted through training specimen, with the employment of nonlinear mapping capability of the artificial neural network. Mapping model with interpolation and extrpolation is gained, laying the foundation for optimum design. The steel structure of high-layer parking system (SSHLPS) is calculated as an instance. A three-layer back-propagation (BP) net including one hidden layer is constructed with nine input nodes and eight output nodes for a five-layer SSHLPS. The three-layer structure optimization result through the mapping model interpolation contrasts with integrity re-analysis, and seven layers structure through the mapping model extrpulation contrasts with integrity re-analysis. Any layer SSHLPS among 1-8 can be calculated with much accuracy. Amount of calculation can also be reduced if it is appfied into the same topological structure, with reduced distortion and assured precision. 展开更多
关键词 Plane model - Space model Artificial neural networks mapping model Optimum design
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大黄灵仙方调控胆管细胞炎症的作用及机制研究 被引量:2
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作者 陈伟棠 俞渊 +6 位作者 庞浇安 李敏鹏 潘孟 陆世锋 杨文 滕金豪 叶桂源 《环球中医药》 CAS 2024年第2期209-216,共8页
目的 观察大黄灵仙方调控白细胞介素-1受体相关激酶(interleukin-1 receptor-associated kinase 1,IRAK)-1和IRAK-4以及成纤维生长因子-3激活酶1MAP3K7结合蛋白2(TGF-3 activator 1 map3k7-binding protein 2,TAB2)的表达水平,探讨其缓... 目的 观察大黄灵仙方调控白细胞介素-1受体相关激酶(interleukin-1 receptor-associated kinase 1,IRAK)-1和IRAK-4以及成纤维生长因子-3激活酶1MAP3K7结合蛋白2(TGF-3 activator 1 map3k7-binding protein 2,TAB2)的表达水平,探讨其缓解胆管细胞炎症的作用机制,为预防肝胆管结石的形成及术后复发提供理论依据。方法 将45只SPF级健康雄性SD大鼠随机分为9组,空白组、模型组、大黄灵仙颗粒组、激活核转录因子κB(nuclear factor kappa-B,NF-κB)阻断剂组、p38丝裂活化蛋白激酶(mitogen-activated protein kinase, MAPK)阻断剂组、NF-κB阻断剂+p38MAPK阻断剂组、NF-κB阻断剂+大黄灵仙颗粒组、p38MAPK阻断剂+大黄灵仙颗粒组、NF-κB阻断剂+p38MAPK阻断剂+大黄灵仙颗粒组,每组大鼠5只。除空白组以外,其余各组大鼠于胆总管处一次性注射5 mg/kg内毒素脂多糖以构建肝内胆管感染动物模型。第7天灌胃结束后取材,取大鼠完整胆管树组织,采用蛋白免疫印迹法和实时荧光定量PCR检测IRAK-1、IRAK-4、TAB2蛋白及mRNA表达量。结果 (1)与空白组比较,模型组IRAK-1、IRAK-4、TAB2蛋白及mRNA表达量升高(P<0.05);(2)与模型组比较,大黄灵仙颗粒组、NF-κB阻断剂组、p38MAPK阻断剂组、NF-κB阻断剂+p38MAPK阻断剂组、NF-κB阻断剂+大黄灵仙颗粒组、IRAK-1、IRAK-4、TAB2的蛋白及mRNA表达量降低(P<0.05),NF-κB阻断剂+p38MAPK阻断剂+大黄灵仙颗粒组IRAK-1、IRAK-4、TAB2蛋白及TAB2 mRNA表达量均显著下降(P<0.05);(3)与大黄灵仙颗粒组比较,NF-κB阻断剂+大黄灵仙颗粒组、NF-κB阻断剂+p38MAPK阻断剂+大黄灵仙颗粒组IRAK-1、IRAK-4、TAB2的蛋白表达量和NF-κB阻断剂+p38MAPK阻断剂组的IRAK-1、IRAK-4、TAB2 mRNA表达量均下降(P<0.05)。结论 大黄灵仙方可缓解内毒素脂多糖诱导的大鼠胆管炎症反应,抑制炎症因子的异常表达,促使胆道恢复至非炎症状态,从而达到预防肝胆管结石的形成及术后复发的目的,其作用机制可能与抑制TLR4/NF-κB/MAPK信号通路下游IRAK-1、IRAK-4和TAB2因子的活化有关。 展开更多
关键词 大黄灵仙方 胆管炎症 肝内胆管结石 白细胞介素-1受体相关激酶1 白细胞介素-1受体相关激酶4 成纤维生长因子-3激活酶1map3KT结合蛋白2
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A kind of noise-induced transition to noisy chaos in stochastically perturbed dynamical system 被引量:3
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作者 Chun-Biao Gan Shi-Xi Yang Hua Lei 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2012年第5期1416-1423,共8页
We investigate a kind of noise-induced transition to noisy chaos in dynamical systems. Due to similar phenomenological structures of stable hyperbolic attractors excited by various physical realizations from a given s... We investigate a kind of noise-induced transition to noisy chaos in dynamical systems. Due to similar phenomenological structures of stable hyperbolic attractors excited by various physical realizations from a given stationary random process, a specific Poincar6 map is established for stochastically perturbed quasi-Hamiltonian system. Based on this kind of map, various point sets in the Poincar6's cross-section and dynamical transitions can be analyzed. Results from the customary Duffing oscillator show that, the point sets in the Poincare's global cross-section will be highly compressed in one direction, and extend slowly along the deterministic period-doubling bifurcation trail in another direction when the strength of the harmonic excitation is fixed while the strength of the stochastic excitation is slowly increased. This kind of transition is called the noise-induced point-overspreading route to noisy chaos. 展开更多
关键词 Stochastic excitation - Dynamical system - Specific Poincare map Noise-induced transition to chaos
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On Common Fixed Point Theorem of Four Self Maps in a Fuzzy Metric Space
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作者 Manthena Prapoorna Manchala Rangamma 《Advances in Pure Mathematics》 2016年第5期303-308,共6页
In the present paper, we show that there exists a unique common fixed point for four self maps in a fuzzy metric space where two of the maps are reciprocally continuous and the other two maps are z-asymptotically comm... In the present paper, we show that there exists a unique common fixed point for four self maps in a fuzzy metric space where two of the maps are reciprocally continuous and the other two maps are z-asymptotically commuting. 展开更多
关键词 T-NORM Fuzzy Metric Space Reciprocally Continuous Z - Asymptotically Commuting maps
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Pattern recognition of seismogenic nodes using Kohonen selforganizing map: example in west and south west of Alborz region in Iran
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作者 Mostafa Allamehzadeh Soma Durudi Leila Mahshadnia 《Earthquake Science》 CSCD 2017年第3期145-155,共11页
Pattern recognition of seismic and mor- phostructural nodes plays an important role in seismic hazard assessment. This is a known fact in seismology that tectonic nodes are prone areas to large earthquake and have thi... Pattern recognition of seismic and mor- phostructural nodes plays an important role in seismic hazard assessment. This is a known fact in seismology that tectonic nodes are prone areas to large earthquake and have this potential. They are identified by morphostructural analysis. In this study, the Alborz region has considered as studied case and locations of future events are forecast based on Kohonen Self-Organized Neural Network. It has been shown how it can predict the location of earthquake, and identifies seismogenic nodes which are prone to earthquake of M5.5+ at the West of Alborz in Iran by using International Institute Earthquake Engineering and Seismology earthquake catalogs data. First, the main faults and tectonic lineaments have been identified based on MZ (land zoning method) method. After that, by using pattern recognition, we generalized past recorded events to future in order to show the region of probable future earthquakes. In other word, hazardous nodes have determined among all nodes by new catalog generated Self-organizing feature maps (SOFM). Our input data are extracted from catalog, consists longitude and latitude of past event between 1980-2015 with magnitude larger or equal to 4.5. It has concluded node D1 is candidate for big earthquakes in comparison with other nodes and other nodes are in lower levels of this potential. 展开更多
关键词 Clustering - Earthquake prediction ~ Self-organizing feature maps (SOFM)
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H20-20调度系统调度台无故障转移问题的分析处理
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作者 孟艳龄 《电力系统通信》 2002年第8期52-53,共2页
介绍了处理调度系统调度台故障的方法 。
关键词 电力调度系统 电力调度台 无故障转移 H20-20map
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芍药苷对小鼠神经病理性痛的影响
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作者 钟昊彤 许东元 +2 位作者 张平 闫清华 李仁淑 《临床麻醉学杂志》 CAS CSCD 北大核心 2023年第4期407-413,共7页
目的探讨芍药苷对小鼠神经病理性痛(NP)的影响。方法选择SPF级雄性BALB/c小鼠40只,4~6周龄,体重20~25 g。将小鼠随机分为四组:假手术组(S组)、坐骨神经套管结扎损伤组(C组)、坐骨神经套管结扎损伤+芍药苷50 mg/kg组(P50组)和坐骨神经套... 目的探讨芍药苷对小鼠神经病理性痛(NP)的影响。方法选择SPF级雄性BALB/c小鼠40只,4~6周龄,体重20~25 g。将小鼠随机分为四组:假手术组(S组)、坐骨神经套管结扎损伤组(C组)、坐骨神经套管结扎损伤+芍药苷50 mg/kg组(P50组)和坐骨神经套管结扎损伤+芍药苷100 mg/kg组(P100组),每组10只。S组仅分离坐骨神经,不进行套管结扎。C组、P50组和P100组行坐骨神经套管结扎术。P50组术后连续14 d腹腔注射芍药苷50 mg/kg,P100组术后连续14 d腹腔注射芍药苷100 mg/kg,S组和C组术后连续14 d腹腔注射相同容量的生理盐水。于术后1、2、5、7、10、14 d测定机械缩足阈值(MWT)和热回缩潜伏期(TWL),于术后13 d行蔗糖偏好实验(SPT)测定糖水消耗百分比,于术后14 d行强迫游泳实验(FST)和悬尾试验(TST)分别测定静止不动时间。于术后14 d取小鼠眼球血,采用ELISA法测定TNF-α、IL-6、IL-1β浓度。采用Western blot法检测海马组织MKP-1蛋白含量、p-ERK/ERK及p-p38/p38比值。采用HE染色观察海马组织CA3区锥体细胞形态,免疫组织化学染色观察海马组织DG区小胶质细胞激活数量。结果与S组比较,C组、P50组和P100组术后2、5、7、10、14 d MWT明显降低、TWL明显缩短,糖水消耗百分比明显降低,FST和TST静止不动时间明显延长,海马组织TNF-α、IL-6和IL-1β浓度、p-ERK/ERK、p-p38/p38比值明显升高,MKP-1蛋白含量明显降低,DG区小胶质细胞激活数量明显增加(P<0.05)。与C组比较,P50组和P100组术后2、5、7、10、14 d MWT明显升高、TWL明显延长,糖水消耗百分比明显升高,FST和TST静止不动时间明显缩短,海马组织TNF-α、IL-6和IL-1β浓度、p-ERK/ERK、p-p38/p38比值明显降低,MKP-1蛋白含量明显升高,DG区小胶质细胞激活数量明显减少(P<0.05)。与P50组比较,P100组术后2、5、7、10、14 d MWT明显升高、TWL明显延长,糖水消耗百分比明显升高,FST和TST静止不动时间明显缩短,海马组织TNF-α、IL-6和IL-1β浓度、p-ERK/ERK、p-p38/p38比值明显降低,MKP-1蛋白含量明显升高,DG区小胶质细胞激活数量明显减少(P<0.05)。结论芍药苷可以缓解小鼠神经病理性痛导致的痛觉过敏和抑郁样行为,降低海马组织炎性因子浓度,升高MKP-1蛋白含量,降低其下游信号分子p-ERK/ERK、p-p38/p38比值,减少小胶质细胞的激活,芍药苷100 mg/kg效果优于50 mg/kg。 展开更多
关键词 芍药苷 map蛋白激酶磷酶-1 抑郁 神经病理性痛
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锂离子电池宽温度区间无析锂快充策略 被引量:8
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作者 周旋 周萍 +5 位作者 郑岳久 韩雪冰 褚政宇 刘金海 杨映华 薛钢 《汽车安全与节能学报》 CAS CSCD 2020年第3期397-405,共9页
为解决锂离子电池快充中存在的安全问题,提出一种标定动力锂离子电池无析锂快充电流Map图的方法。采用参比电极作为监测负极电位的手段,依据电势判据设立不析锂负极电位阈值,通过调节不同充电起始温度点,获取5条不同起始温度点的快充策... 为解决锂离子电池快充中存在的安全问题,提出一种标定动力锂离子电池无析锂快充电流Map图的方法。采用参比电极作为监测负极电位的手段,依据电势判据设立不析锂负极电位阈值,通过调节不同充电起始温度点,获取5条不同起始温度点的快充策略曲线,运用分段线性插值方法,得到荷电状态-温度-电流等高线图(SOC-T-I Map)。为了验证Map图的快速性和无析锂,设计快速性对比实验和25℃快充策略循环实验。结果表明,25℃快充策略比25℃恒流1 C、1.5 C充电时间分别缩短45.3%、18.0%;200次快充循环后,弛豫电压微分曲线无极小值且电池维持99.7%的容量保持率,表明无金属锂析出。该文标定电流Map图的方法提供了挖掘电池快充能力的思路,并且标定的Map图适合电动汽车在多种不同工况下快充。 展开更多
关键词 电动汽车 锂离子电池 无析锂快充 参比电极 荷电状态-温度-电流等高线图(SOC-T-I map)
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牛副结核分枝杆菌(MAP)MAP3290-1595串联蛋白的原核表达及反应原性检测报告
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作者 张哲 周子恒 +5 位作者 蔡卓轩 张彦红 杨艳 钱天皓 李岩 盛金良 《当代畜牧》 2020年第2期25-29,共5页
原核表达纯化牛副结核分枝杆菌MAP3290-1595蛋白,为检测牛副结核分枝杆菌提供了新的材料。笔者通过基因串联融合MAP3290和MAP1595基因,获得MAP3290-1595基因,并使用DNAMAN软件对MAP3290-1595基因序列进行分析;构建重组质粒pET30aMAP3290... 原核表达纯化牛副结核分枝杆菌MAP3290-1595蛋白,为检测牛副结核分枝杆菌提供了新的材料。笔者通过基因串联融合MAP3290和MAP1595基因,获得MAP3290-1595基因,并使用DNAMAN软件对MAP3290-1595基因序列进行分析;构建重组质粒pET30aMAP3290-1595,将其转化至E.coli BL21(DE3)感受态细胞中,使用IPTG诱导目的蛋白表达;使用亲和层析法对目的蛋白进行亲和纯化,从而获得MAP3290-1595蛋白;利用Western Blot方法对该蛋白进行质检。成功表达纯化出MAP3290-1595蛋白。该蛋白大小为43 k Da,浓度为0.430 mg/mL,纯度大于90%。本试验成功获得MAP3290-1595蛋白,利用Western Blot和间接ELISA法检测该蛋白的反应原性,与牛副结核阴性血清反应良好,得出该蛋白具有良好的反应原性,为建立牛副结核分支杆菌检测方法提供材料。 展开更多
关键词 牛副结核分枝杆菌 map3290-1595蛋白 原核表达
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Fixed Point Theorems of the Iterated Function Systems
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作者 Ji You-qing Liu Zhi Ri Song-il 《Communications in Mathematical Research》 CSCD 2016年第2期142-150,共9页
In this paper, we present some fixed point theorems of iterated function systems consisting of α-ψ-contractive type mappings in Fractal space constituted by the compact subset of metric space and iterated function s... In this paper, we present some fixed point theorems of iterated function systems consisting of α-ψ-contractive type mappings in Fractal space constituted by the compact subset of metric space and iterated function systems consisting of Banach contractive mappings in Fractal space constituted by the compact subset of generalized metric space, which is Mso extensively applied in topological dynamic system. 展开更多
关键词 fixed point α-ψ-contractive mapping iterated function system gener-alized metric space
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ON THE m^(th) ORDER DIFFERENCE SEQUENCE SPACE OF GENERALIZED WEIGHTED MEAN AND COMPACT OPERATORS 被引量:1
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作者 Metin BASARIR Emrah Evren KARA 《Acta Mathematica Scientia》 SCIE CSCD 2013年第3期797-813,共17页
In this article, using generalized weighted mean and difference matrix of order m, we introduce the paranormed sequence space l(u, v, p; △(m)), which consist of the sequences whose generalized weighted △(m)-di... In this article, using generalized weighted mean and difference matrix of order m, we introduce the paranormed sequence space l(u, v, p; △(m)), which consist of the sequences whose generalized weighted △(m)-difference means are in the linear space l(p) defined by I.J.Maddox. Also, we determine the basis of this space and compute its α-, β- and γ-duals. Further, we give the characterization of the classes of matrix mappings from l(u, v, p, △(m)) to l∞, c, and co. Finally, we apply the Hausdorff measure of noncompacness to characterize some classes of compact operators given by matrices on the space lp(U, v, △(m)) (1 ≤ p 〈 ∞). 展开更多
关键词 Paranormed sequence spaces difference sequence spaces weighted mean α- β γ-duals matrix mappings Hausdorff measure of noncompactness compactoperators
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测绘e系统的开发与应用
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作者 方门福 曾联斌 +2 位作者 邓作文 梁守宏 李林芳 《测绘与空间地理信息》 2005年第2期71-72,76,共3页
重点介绍进行具有自主知识产权的不需AutoCAD支持的纯国产数字化测图软件测绘e系统的必要性、技术路线、主要功能及特点、应用情况及未来的发展方向等。
关键词 测绘 技术路线 数字化测图 软件系统 土方计算
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The Membrane-Associated Sec1/Munc18 KEULE is Required for Phragmoplast Microtubule Reorganization During Cytokinesis in Arabidopsis 被引量:1
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作者 Alexander Steiner Lin Muller +7 位作者 Katarzyna Rybak Vera Vodermaier Eva Facher Martha Thellmann Raksha Ravikumar Gerhard Wanner Marie-Theres Hauser Farhah F. Assaad 《Molecular Plant》 SCIE CAS CSCD 2016年第4期528-540,共13页
Cytokinesis, the partitioning of the cytoplasm following nuclear division, requires extensive coordination between membrane trafficking and cytoskeletal dynamics. In plants, the onset of cytokinesis is characterized b... Cytokinesis, the partitioning of the cytoplasm following nuclear division, requires extensive coordination between membrane trafficking and cytoskeletal dynamics. In plants, the onset of cytokinesis is characterized by the assembly of a bipolar microtubule array, the phragmoplast, and of a transient membrane compartment, the cell plate. Little is known about the coordination between membrane deposition at the cell plate and the dynamics of phragmoplast microtubules. In this study, we monitor the localization dynamics of microtubule and membrane markers throughout cytokinesis. Our spatiotemporal resolution is consistent with the general view that microtubule dynamics drive membrane movements. Nonetheless, we provide evidence for active sorting at the cell plate and show that this is, at least in part, mediated by the TRAPPII tethering complex. We also characterize phragmoplast microtubule organization and cell plate formation in a suite of cytokinesis-defective mutants. Of four mutant lines with defects in phragmoplast microtubule organization, only morl microtubule-associated mutants exhibited aberrant cell plates. Conversely, the mutants with the strongest impairment in phragmoplast microtubule reorganization are keule alleles, which have a primary defect in membrane fusion. Our findings identify the SEC1/Munc18 protein KEULE as a central regulatory node in the coordination of membrane and microtubule dynamics during plant cytokinesis. 展开更多
关键词 CYTOKINESIS cell plate phragmoplast microtubule KEULE KNOLLE PLEIADE/map65-3
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