A sensitive and reliable method was developed and validated for the simu1taneous determination of 6 carbamate pesticides and relevant metabolites based on the modified fast QuEChERS(quick,easy,cheap,effective,rugged a...A sensitive and reliable method was developed and validated for the simu1taneous determination of 6 carbamate pesticides and relevant metabolites based on the modified fast QuEChERS(quick,easy,cheap,effective,rugged and safe)method combined with ultra-performance 1iquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The method was also applied in the analysis of 23 commonly consumed oilseeds and oil samples.The sample was extracted with acetonitrile for three times and then the extracted solution was filtered directly though 250 mg precipitated silica adsorbent(PSA)and 250 mg Cis The mobile phase was water(containing 0.1%formic acid)and acetonitrile.The extemal standard protocols were adopted to validate the method in terms of recovery,1inearity,precision,sensitivity,and relative standard deviation(RSD)under the optimized conditions.The linear calibration curves with different ranges were established with R^(2)>0.9973,limit of detections(LODs)ranged from 0.100μg/kg to 20000μg/kg and limit of quantitations(LOQs)ranged from 0.300μg/kg to 6.000μg/kg.The LODs meets the detection requirement for the maximum residue limits in practical samples.Meanwhile,the recoveries ranged from 60.2%to 116.6%with associated RSDs ranged from 0.33%to 5.35%in 10 oilseeds matrices and ranged from 61.9%to 116.9%with RSDs ranged from 0.30%to 5.02%in 13 oil matrices.These features do make the UPLC-MSMS an ideal analytical platform for accurate and high throughput analysis of carbamates residues in the complex matrix.展开更多
基金This work was supported by the Postdoctoral Science Foundation of Anhui Province(2020B412),the project of Science and Technology Program of State Administration for Market Regulation(2020MK049)the project of Science and Technology Program of Administration for Market Regulation of Anhui Province(2019MK037).
文摘A sensitive and reliable method was developed and validated for the simu1taneous determination of 6 carbamate pesticides and relevant metabolites based on the modified fast QuEChERS(quick,easy,cheap,effective,rugged and safe)method combined with ultra-performance 1iquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The method was also applied in the analysis of 23 commonly consumed oilseeds and oil samples.The sample was extracted with acetonitrile for three times and then the extracted solution was filtered directly though 250 mg precipitated silica adsorbent(PSA)and 250 mg Cis The mobile phase was water(containing 0.1%formic acid)and acetonitrile.The extemal standard protocols were adopted to validate the method in terms of recovery,1inearity,precision,sensitivity,and relative standard deviation(RSD)under the optimized conditions.The linear calibration curves with different ranges were established with R^(2)>0.9973,limit of detections(LODs)ranged from 0.100μg/kg to 20000μg/kg and limit of quantitations(LOQs)ranged from 0.300μg/kg to 6.000μg/kg.The LODs meets the detection requirement for the maximum residue limits in practical samples.Meanwhile,the recoveries ranged from 60.2%to 116.6%with associated RSDs ranged from 0.33%to 5.35%in 10 oilseeds matrices and ranged from 61.9%to 116.9%with RSDs ranged from 0.30%to 5.02%in 13 oil matrices.These features do make the UPLC-MSMS an ideal analytical platform for accurate and high throughput analysis of carbamates residues in the complex matrix.
