The“depict”strategy for discovering new compounds in complex matrices:Lycibarbarspermidines as a case

The comprehensive detection and identification of active ingredients in complex matrices is a crucial challenge.Liquid chromatography coupled with high-resolution mass spectrometry(LC-HRMS)is the most prominent analytical platform for the exploration of novel active compounds from complex matrices.However,the LC-HRMS-based analysis workflow suffers from several bottleneck issues,such as trace content of target compounds,limited acquisition for fragment information,and uncertainty in interpreting relevant MS2 spectra.Lycibarbarspermidines are vital antioxidant active ingredients in Lycii Fructus,while the reported structures are merely focused on dicaffeoylspermidines due to their low content.To comprehensively detect the new structures of lycibarbarspermidine derivatives,a“depict”strategy was developed in this study.First,potential new lycibarbarspermidine derivatives were designed according to the biosynthetic pathway,and a comprehensive database was established,which enlarged the coverage of lycibarbarspermidine derivatives.Second,the polarity-oriented sample preparation of potential new compounds increased the concentration of the target compounds.Third,the construction of the molecular network based on the fragmentation pathway of lycibarbarspermidine derivatives broadened the comprehensiveness of identification.Finally,the weak response signals were captured by data-dependent scanning(DDA)followed by parallel reaction monitoring(PRM),and the efficiency of acquiring MS2 fragment ions of target compounds was significantly improved.Based on the integrated strategy above,210 lycibarbarspermidine derivatives were detected and identified from Lycii Fructus,and in particular,170 potential new compounds were structurally characterized.The integrated strategy improved the sensitivity of detection and the coverage of low-response components,and it is expected to be a promising pipeline for discovering new compounds.

Highly Aligned Ternary Nanofiber Matrices Loaded with MXene Expedite Regeneration of Volumetric Muscle Loss

Current therapeutic approaches for volumetric muscle loss(VML)face challenges due to limited graft availability and insufficient bioactivities.To overcome these limitations,tissue-engineered scaffolds have emerged as a promising alternative.In this study,we developed aligned ternary nanofibrous matrices comprised of poly(lactide-co-ε-caprolactone)integrated with collagen and Ti_(3)C_(2)T_(x)MXene nanoparticles(NPs)(PCM matrices),and explored their myogenic potential for skeletal muscle tissue regeneration.The PCM matrices demonstrated favorable physicochemical properties,including structural uniformity,alignment,microporosity,and hydrophilicity.In vitro assays revealed that the PCM matrices promoted cellular behaviors and myogenic differentiation of C2C12 myoblasts.Moreover,in vivo experiments demonstrated enhanced muscle remodeling and recovery in mice treated with PCM matrices following VML injury.Mechanistic insights from next-generation sequencing revealed that MXene NPs facilitated protein and ion availability within PCM matrices,leading to elevated intracellular Ca^(2+)levels in myoblasts through the activation of inducible nitric oxide synthase(i NOS)and serum/glucocorticoid regulated kinase 1(SGK1),ultimately promoting myogenic differentiation via the m TOR-AKT pathway.Additionally,upregulated i NOS and increased NO–contributed to myoblast proliferation and fiber fusion,thereby facilitating overall myoblast maturation.These findings underscore the potential of MXene NPs loaded within highly aligned matrices as therapeutic agents to promote skeletal muscle tissue recovery.

Benzodiazepines in complex biological matrices: Recent updates on pretreatment and detection methods

Benzodiazepines(BDZs)are used in clinics for anxiolysis,anticonvulsants,sedative hypnosis,and muscle relaxation.They have high consumptions worldwide because of their easy availability and potential addiction.They are often used for suicide or criminal practices such as abduction and drug-facilitated sexual assault.The pharmacological effects of using small doses of BDZs and their detections from complex biological matrices are challenging.Efficient pretreatment methods followed by accurate and sensitive detections are necessary.Herein,pretreatment methods for the extraction,enrichment,and preconcentration of BDZs as well as the strategies for their screening,identification,and quantitation developed in the past five years have been reviewed.Moreover,recent advances in various methods are summarized.Characteristics and advantages of each method are encompassed.Future directions of the pretreatment and detection methods for BDZs are also reviewed.

Bounds for Polynomial’s Roots from Fiedler and Sparse Companion Matrices for Submultiplicative Matrix Norms

We use submultiplicative companion matrix norms to provide new bounds for roots for a given polynomial P(X) over the field C[X]. From a n×n Fiedler companion matrix C, sparse companion matrices and triangular Hessenberg matrices are introduced. Then, we identify a special triangular Hessenberg matrix Lr, supposed to provide a good estimation of the roots. By application of Gershgorin’s theorems to this special matrix in case of submultiplicative matrix norms, some estimations of bounds for roots are made. The obtained bounds have been compared to known ones from the literature precisely Cauchy’s bounds, Montel’s bounds and Carmichel-Mason’s bounds. According to the starting formel of Lr, we see that the more we have coefficients closed to zero with a norm less than 1, the more the Sparse method is useful.

Materials Selection of Thermoplastic Matrices of Natural Fibre Composites for Cyclist Helmet Using an Integration of DMAIC Approach in Six Sigma Method Together with Grey Relational Analysis Approach

Natural fibre reinforced polymer composite(NFRPC)materials are gaining popularity in the modern world due to their eco-friendliness,lightweight nature,life-cycle superiority,biodegradability,low cost,and noble mechanical properties.Due to the wide variety of materials available that have comparable attributes and satisfy the requirements of the product design specification,material selection has become a crucial component of design for engineers.This paper discusses the study’s findings in choosing the suitable thermoplastic matrices of Natural Fibre Composites for Cyclist Helmet utilising the DMAIC,and GRA approaches.The results are based on integrating two decision methods implemented utilising two distinct decision-making approaches:qualitative and quantitative.This study suggested thermoplastic polyethylene as a particularly ideal matrix in composite cyclist helmets during the selection process for the best thermoplastic matrices material using the 6σtechnique,with the decision based on the highest performance,the lightest weight,and the most environmentally friendly criteria.The DMAIC and GRA approach significantly influenced the material selection process by offering different tools for each phase.In the future study,selection technique may have been more exhaustive if more information from other factors had been added.

E-matrices and Several Necessary and Sufficient Conditions

In this paper,we define a kind of sguare matrices which is called E-matrices,and give several necessary and sufficient conditions for E-matrices.

Criteria Condition for Generalized Diagonally Dominant Matrices

In this paper, we provide some new necessary and sufficient conditions for generalized diagonally dominant matrices and also obtain some criteria for nongeneralized dominant matrices.

The Explicit Expressions of Level- k(r1 ,r2 ,… ,rk) Circulant Matrices and Their Some Properties

In this paper,we give the explicit expressions of level k (r 1,r 2,…,r k) circulant matrices of order n 1n 2…n k,and the explicit expressions for the eigenvalues,the determinants and the inverse matrices of the kind level k (r 1,r 2,…,r k) circulant matrices are derived,and it is also proved that the sort of matrices are diagonalizable.

The Monotonicity Problems for Generalized Inverses of Matrices in H (n, ≥)

On the basis of the paoers[3—7],this paper study the monotonicity problems for the positive semidefinite generalized inverses of the positive semidefinite self-conjugate matrices of quaternions in the Lowner partial order,give the explicit formulations of the monotonicity solution sets A{1;≥,T_1;≤B^(1)}and B{1;≥,T_2≥A^(1)}for the(1)-inverse,and two results of the monotonicity charac teriaztion for the(1,2)-inverse.

The Explicit Expressions of Level-k Circulant Matrices of Type(n_1,n_2.…,n_k) and Their Some Properties

In this paper, we give the explicit expressions of level-k circulant matrices of type (n1,n2,…nk) and of order n1n2…nk,and the explicit expressions for the eigenvalues,the determinants and the inverse matrices of the kind level-k circulant matrices are derived,and it is also proved that the sort matrices are unitarily diagonalizable.

BEST APPROXIMATION BY NORMAL MATRICES WITH SPECTRAL CONSTRAINTS

The problem of best approximating, a given square complex matrix in the Frobenius norm by normal matrices under a given spectral restriction is considered. The ne cessary and sufficient condition for the solvability of the problem is given. A numerical algorithm for solving the problem is provided and a numerical example is presented.

Linear Maps Preserving Idempotency of Products of Matrices on Upper Triangular Matrix Algebras

Let Tn be the algebra of all n × n complex upper triangular matrices. We give the concrete forms of linear injective maps on Tn which preserve the nonzero idempotency of either products of two matrices or triple Jordan products of two matrices.

Some Remarks On E＇-matrices

The class of E＇-matrices introduced in [4] is a subclass of Q0-matrices whose proper principal submatrices are Q--matrices. In this paper, we investigate the relation of the class E＇ to other known matrix classes and obtain a series of necessary and sufficient conditions for E＇-matrices.

Preparation and Characterization of Collagen-GAGs Bioactive Matrices for Tissue Engineering

Collagen materials were crosslinked by 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) in the presence of chondroitin sulfate (CS), one of glycosaminoglycans (GAGS). PVA and chitosan were also blended with collagen. The physical and chemical properties of the matrices were characterized by SEM, DSC, and ESCA. L929 cells were implanted on the matrices to show the cytotoxic and the biological characters of the materials. The results indicate that EDC is an effective and non-cytotoxic cross-link reagent, which can replace the common dialdehyde reagent. The attachment of CS can improve the stability of collagen and accelerate cell growth. The addition of PVA can prepare porous matrices with smaller bore size. There are reactions between the chitosan and collagen, and the composite has good biological character. The presence of chitosan can also increase the amount of incorporated CS.

Formulation and evaluation the bioadhesive properties of drug delivery system based on PEGylated mucin matrices

Objective:To formulate and evaluate the application of PEGylated mucin in oral bioadhesive drug(OBD) delivery system.Methods:The bioadhesive strength of different formulation ratios of polyethylene glycol(PEG) and mucin was evaluated by tensiometry.Co polymer matrices containing mucin and PEG(PEGylated mucin) in the following ratios of 1:1(A),2:1(B),1:2(C), 0:1(D) and 1:0(E) were prepared by co-precipitation.Microparticles were prepared from the matrices using a size reduction technique.Five different samples were prepared using direct mixtures in the appropriate solvenfs having mucin and PEG ratios of 1:1,2:1,1:2,0:1, 1:0 and labeled as(A-E).The matrices were evaluated for their flow properties and the in vitro bioadhesion characteristics of the samples were examined.Results:The range of flow rates of the matrices was 1.35-2.23 g/sec.The angle of repose was in the range of 37.3-39.9 degree.The bulk and tapped densities were within the ranges of 0.41- 0.49.The Hausner’s quotient(HQ) were 1.12, 1.24,1.14,1.25 and 1.2 for matrix batches A-E respectively,The bioadhesive strength of polymer matrices appeared to be directly related to amount of mucin.The order of bioadhesive strength is 2:1】1:1】1:0】1:2】0:1 of PEG:mucin in both simulated intestine fluid(SEP) and simulated gastrointestinal fluid(SGF).The physical properties of the micrometrics properties of the matrices were within the accepted values.Conclusions:OBD preparation containing PEGylated mucin can be prepared by direct compression and be used in drug delivery to the oral cavity.

Sparse Transform Matrices and Their Application in the Calculation of Electromagnetic Scattering Problems

When compressed sensing is introduced into the moment method,a 3D electromagnetic scattering problem over a wide angle can be solved rapidly,and the selection of sparse basis has a direct influence on the performance of this algorithm,especially the number of measurements.We set up five sparse transform matrices by discretization of five types of classical orthogonal polynomials,i.e.,Legendre,Chebyshev,the second kind of Chebyshev,Laguerre,and Hermite polynomials.Performances of the algorithm using these matrices are compared via numerical experiments,and the results show that some of them obviously work excellently and can accelerate wide angle scattering analysis greatly.

Generalized Drazin spectrum of operator matrices

Let A ∈ B（X） and B ∈ B（Y）, Me be an operator on Banach space X ＋ Y given by Mc =（A C 0 B）A generalized Drazin spectrum defined by σgD（T） = {λ∈C ： T-λI is not generalized Drazin invertible} is considered in this paper. It is shown that

Synchronization of complex switched delay dynamical networks with simultaneously diagonalizable coupling matrices

This paper studies local exponential synchronization of complex delayed networks with switching topology via switched system stability theory. First, by a common unitary matrix, the problem of synchronization is transformed into the stability analysis of some linear switched delay systems. Then, when all subnetworks are synchronizable, a delay-dependent sufficient condition is given in terms of linear matrix inequalities （LMIs） which guarantees the solvability of the synchronization problem under an average dwell time scheme. We extend this result to the case that not all subnetworks are synchronizable. It is shown that in addition to average dwell time, if the ratio of the total activation time of synchronizable and non-synchronizable subnetworks satisfy an extra condition, then the problem is also solvable. Two numerical examples of delayed dynamical networks with switching topology are given, which demonstrate the effectiveness of obtained results.

Development of damage probability matrices based on Greek earthquake damage data

A comprehensive study is presented for empirical seismic vulnerability assessment of typical structural types, representative of the building stock of Southern Europe, based on a large set of damage statistics. The observational database was obtained from post-earthquake surveys carried out in the area struck by the September 7, 1999 Athens earthquake. After analysis of the collected observational data, a unified damage database has been created which comprises 180,945 damaged buildings from/after the near-field area of the earthquake. The damaged buildings are classified in specific structural types, according to the materials, seismic codes and construction techniques in Southern Europe. The seismic demand is described in terms of both the regional macroseismic intensity and the ratio αg/ao, where αg is the maximum peak ground acceleration （PGA） of the earthquake event and ao is the unique value PGA that characterizes each municipality shown on the Greek hazard map. The relative and cumulative frequencies of the different damage states for each structural type and each intensity level are computed in terms of damage ratio. Damage probability matrices （DPMs） and vulnerability curves are obtained for specific structural types. A comparison analysis is fulfilled between the produced and the existing vulnerability models.

Construction of protograph LDPC codes with circular generator matrices

The application of protograph low density parity check （LDPC） codes involves the encoding complexity problem. Since the generator matrices are dense, and if the positions of ＂1＂ s are irregularity, the encoder needs to store every ＂1＂ of the generator matrices by using huge chip area. In order to solve this problem, we need to design the protograph LDPC codes with circular generator matrices. A theorem concerning the circulating property of generator matrices of nonsingular protograph LDPC codes is proposed. The circulating property of generator matrix of nonsingular protograph LDPC codes can be obtained from the corresponding quasi-cyclic parity check matrix. This paper gives a scheme of constructing protograph LDPC codes with circulating generator matrices, and it reveals that the fast encoding algorithm of protograph LDPC codes has lower encoding complexity under the condition of the proposed theorem. Simulation results in ad- ditive white Gaussian noise （AWGN） channels show that the bit error rate （BER） performance of the designed codes based on the proposed theorem is much better than that of GB20600 LDPC codes and Tanner LDPC codes.