BACKGROUND Juvenile dermatomyositis(JDM)is an idiopathic inflammatory myopathy that occurs in childhood.It is characterized by muscle weakness and a characteristic rash.Previous literature reports have rarely describe...BACKGROUND Juvenile dermatomyositis(JDM)is an idiopathic inflammatory myopathy that occurs in childhood.It is characterized by muscle weakness and a characteristic rash.Previous literature reports have rarely described JDM with severe skin ulcers and infections.CASE SUMMARY Herein,we describe a case of a 2-year-old female patient who suffered from JDM,whose myositis-specific autoantibodies were positive for anti-nuclear matrix protein 2 antibody,with progressively worsening skin ulcers and severe infections.The patient was treated with glucocorticoids and various immunosuppressants.Nevertheless,further progression of the disease and the combination of primary disease and severe infection in the later period were fatal.CONCLUSION In children,anti-nuclear matrix protein 2+JDM combined with skin ulcers often indicates severe disease.In such cases,personalized treatment for the primary disease and infection prevention and control are essential.展开更多
The evolution of protein family is a process along the time course, thus any mathematical methods that can describe a process over time could be possible to describe an evolutionary process. In our previously concept-...The evolution of protein family is a process along the time course, thus any mathematical methods that can describe a process over time could be possible to describe an evolutionary process. In our previously concept-initiated study, we attempted to use the differential equation to describe the evolution of hemagglutinins from influenza A viruses, and to discuss various issues related to the building of differential equation. In this study, we attempted not only to use the differential equation to describe the evolution of matrix protein 2 family from influenza A virus, but also to use the analytical solution to fit its evolutionary process. The results showed that the fitting was possible and workable. The fitted model parameters provided a way to further determine the evolutionary dynamics and kinetics, a way to more precisely predict the time of occurrence of mutation, and a way to figure out the interaction between protein family and its environment.展开更多
Objective： Nuclear matrix protein is tissue, cell-type specific, and tumor-relative. It plays an important role in the regulation of intranuclear processes. Some researches also showed that a c-erbB-2 promoter-specif...Objective： Nuclear matrix protein is tissue, cell-type specific, and tumor-relative. It plays an important role in the regulation of intranuclear processes. Some researches also showed that a c-erbB-2 promoter-specific DNA-binding nuclear matrix protein is present only in malignant human breast tissues and induces mitogenesis and cell surface expression of the c-erbB-2 protein in resting NIH/3T3 cells. But it is not clear that how it in colon adenocarcinomas. Methods： Two-dimensional gel electrophoretic method was used for NMP identification and immunohistochemistry was used for c-erbB-2 detection in 12 cases of colon adenocarcinomas and matched adjacent normal colon tissues. Results： 5 different nuclear matrix proteins （named C1-C5） were identified in 12 colon adenocarcinoma specimens, but not in the matched adjacent normal colon tissues; 3 nuclear matrix proteins （named N1-N3） were identified in all 12 matched adjacent normal colon tissues, but not in colon adenocarcinoma specimens. A nuclear matrix protein （named N4） was detected in all of 9 moderated-well differentiated adenocarcinomas and all 12 matched adjacent normal colon tissues, but not in 3 poor-differentiated adenocarcinomas. All of the 10 colon adenocarcinomas which had the nuclear matrix protein C4 were c-erbB-2 expression positive. Conclusion： The data suggest that there are specific nuclear matrix proteins in colon adenocarcinomas and its subtypes, which maybe valuable to serve as markers of colon adenocarcinomas in future. Nuclear matrix protein C4 probably is a c-erbB-2 promotor-specific nuclear matrix protein in colon adenocarcinomas, and may induce the expression of c-erbB-2.展开更多
This study examined the effects of retinoic acid （RA）, PD98059, SP600125 and SB203580 on the hyperoxia-induced expression and regulation of matrix metalloproteinase-2 （MMP-2） and metalloproteinase-2 （TIMP-2） in ...This study examined the effects of retinoic acid （RA）, PD98059, SP600125 and SB203580 on the hyperoxia-induced expression and regulation of matrix metalloproteinase-2 （MMP-2） and metalloproteinase-2 （TIMP-2） in premature rat lung fibroblasts （LFs）. LFs were exposed to hyperoxia or room air for 12 h in the presence of RA and the kinase inhibitors PD98059 （ERK1/2）, SP600125 （JNK1/2） and SB203580 （p38） respectively. The expression levels of MMP-2 and TIMP-2 mRNA were detected by semi-quantitative reverse transcription polymerase chain reaction （RT-PCR）. MMP-2 activity was measured by zymography. The amount of p-ERK1/2, REK1/2, p-JNK1/2, JNK1/2, p-p38 and p38 was determined by Western blotting. The results showed that： （1） PD98059, SP600125 and SB203580 significantly inhibited p-ERK1/2, p-JNK1/2 and p-p38 respectively in LFs; （2） The expression of MMP-2 mRNA in LFs exposed to hyperoxia was decreased after treatment with RA, SP600125 and SB203580 respectively （P0.01 or 0.05）, but did not change after treatment with PD98059 （P0.05）. Meanwhile, RA, PD98059, SP600125 and SB203580 had no effect on the expression of TIMP-2 mRNA in LFs exposed to room air or hyperoxia （P0.05）; （3） The expression of pro- and active MMP-2 experienced no change after treatment with RA or SP600125 in LFs exposed to room air （P0.05）, but decreased remarkably after hyperoxia （P0.01 or 0.05）. SB203580 inhibited the expression of pro- and active MMP-2 either in room air or under hyperoxia （P0.01）. PD98059 exerted no effect on the expression of pro- and active MMP-2 （P0.05）. It was suggested that RA had a protective effect on hyperoxia-induced lung injury by down-regulating the expression of MMP-2 through decreasing the JNK and p38 activation in hyperoxia.展开更多
抗核基质蛋白2(nuclear matrix protein 2,NXP2)抗体阳性的皮肌炎是特发性炎性肌病的一种,除肌无力、皮肤改变的典型表现外,还有钙质沉着、皮下水肿、严重的肌痛及吞咽困难等表现。本文报道1例具有典型临床及影像学表现、随访17个月后...抗核基质蛋白2(nuclear matrix protein 2,NXP2)抗体阳性的皮肌炎是特发性炎性肌病的一种,除肌无力、皮肤改变的典型表现外,还有钙质沉着、皮下水肿、严重的肌痛及吞咽困难等表现。本文报道1例具有典型临床及影像学表现、随访17个月后复查抗NXP2抗体为阴性的患者。1病例资料患者,男,27岁,因“肢体疼痛无力1月余,加重2周”于2019年6月8日就诊于我科。患者于入院1月前无明显诱因出现肢体疼痛,腰部疼痛症状明显。3周前出现肢体无力感,伴面部皮疹,鼻翼两侧为著。2周前出现全身浮肿,抬头及转颈无力,双上肢抬起费力,坐起费力,行走约50米后肌肉无力酸痛明显,伴双上肢近端、双侧髋部、双膝关节及前胸部皮疹,伴发热,化验示肌酸激酶7500 U/L,行肌电图示肌源性损害,门诊按“肌病”收至我科。患者自发病以来,饮食睡眠可,二便如常,体重增加约10千克。展开更多
文摘BACKGROUND Juvenile dermatomyositis(JDM)is an idiopathic inflammatory myopathy that occurs in childhood.It is characterized by muscle weakness and a characteristic rash.Previous literature reports have rarely described JDM with severe skin ulcers and infections.CASE SUMMARY Herein,we describe a case of a 2-year-old female patient who suffered from JDM,whose myositis-specific autoantibodies were positive for anti-nuclear matrix protein 2 antibody,with progressively worsening skin ulcers and severe infections.The patient was treated with glucocorticoids and various immunosuppressants.Nevertheless,further progression of the disease and the combination of primary disease and severe infection in the later period were fatal.CONCLUSION In children,anti-nuclear matrix protein 2+JDM combined with skin ulcers often indicates severe disease.In such cases,personalized treatment for the primary disease and infection prevention and control are essential.
文摘The evolution of protein family is a process along the time course, thus any mathematical methods that can describe a process over time could be possible to describe an evolutionary process. In our previously concept-initiated study, we attempted to use the differential equation to describe the evolution of hemagglutinins from influenza A viruses, and to discuss various issues related to the building of differential equation. In this study, we attempted not only to use the differential equation to describe the evolution of matrix protein 2 family from influenza A virus, but also to use the analytical solution to fit its evolutionary process. The results showed that the fitting was possible and workable. The fitted model parameters provided a way to further determine the evolutionary dynamics and kinetics, a way to more precisely predict the time of occurrence of mutation, and a way to figure out the interaction between protein family and its environment.
文摘Objective： Nuclear matrix protein is tissue, cell-type specific, and tumor-relative. It plays an important role in the regulation of intranuclear processes. Some researches also showed that a c-erbB-2 promoter-specific DNA-binding nuclear matrix protein is present only in malignant human breast tissues and induces mitogenesis and cell surface expression of the c-erbB-2 protein in resting NIH/3T3 cells. But it is not clear that how it in colon adenocarcinomas. Methods： Two-dimensional gel electrophoretic method was used for NMP identification and immunohistochemistry was used for c-erbB-2 detection in 12 cases of colon adenocarcinomas and matched adjacent normal colon tissues. Results： 5 different nuclear matrix proteins （named C1-C5） were identified in 12 colon adenocarcinoma specimens, but not in the matched adjacent normal colon tissues; 3 nuclear matrix proteins （named N1-N3） were identified in all 12 matched adjacent normal colon tissues, but not in colon adenocarcinoma specimens. A nuclear matrix protein （named N4） was detected in all of 9 moderated-well differentiated adenocarcinomas and all 12 matched adjacent normal colon tissues, but not in 3 poor-differentiated adenocarcinomas. All of the 10 colon adenocarcinomas which had the nuclear matrix protein C4 were c-erbB-2 expression positive. Conclusion： The data suggest that there are specific nuclear matrix proteins in colon adenocarcinomas and its subtypes, which maybe valuable to serve as markers of colon adenocarcinomas in future. Nuclear matrix protein C4 probably is a c-erbB-2 promotor-specific nuclear matrix protein in colon adenocarcinomas, and may induce the expression of c-erbB-2.
基金supported by a grant from the Nature Sciences Foundation of China (No. 30872795)
文摘This study examined the effects of retinoic acid （RA）, PD98059, SP600125 and SB203580 on the hyperoxia-induced expression and regulation of matrix metalloproteinase-2 （MMP-2） and metalloproteinase-2 （TIMP-2） in premature rat lung fibroblasts （LFs）. LFs were exposed to hyperoxia or room air for 12 h in the presence of RA and the kinase inhibitors PD98059 （ERK1/2）, SP600125 （JNK1/2） and SB203580 （p38） respectively. The expression levels of MMP-2 and TIMP-2 mRNA were detected by semi-quantitative reverse transcription polymerase chain reaction （RT-PCR）. MMP-2 activity was measured by zymography. The amount of p-ERK1/2, REK1/2, p-JNK1/2, JNK1/2, p-p38 and p38 was determined by Western blotting. The results showed that： （1） PD98059, SP600125 and SB203580 significantly inhibited p-ERK1/2, p-JNK1/2 and p-p38 respectively in LFs; （2） The expression of MMP-2 mRNA in LFs exposed to hyperoxia was decreased after treatment with RA, SP600125 and SB203580 respectively （P0.01 or 0.05）, but did not change after treatment with PD98059 （P0.05）. Meanwhile, RA, PD98059, SP600125 and SB203580 had no effect on the expression of TIMP-2 mRNA in LFs exposed to room air or hyperoxia （P0.05）; （3） The expression of pro- and active MMP-2 experienced no change after treatment with RA or SP600125 in LFs exposed to room air （P0.05）, but decreased remarkably after hyperoxia （P0.01 or 0.05）. SB203580 inhibited the expression of pro- and active MMP-2 either in room air or under hyperoxia （P0.01）. PD98059 exerted no effect on the expression of pro- and active MMP-2 （P0.05）. It was suggested that RA had a protective effect on hyperoxia-induced lung injury by down-regulating the expression of MMP-2 through decreasing the JNK and p38 activation in hyperoxia.
文摘抗核基质蛋白2(nuclear matrix protein 2,NXP2)抗体阳性的皮肌炎是特发性炎性肌病的一种,除肌无力、皮肤改变的典型表现外,还有钙质沉着、皮下水肿、严重的肌痛及吞咽困难等表现。本文报道1例具有典型临床及影像学表现、随访17个月后复查抗NXP2抗体为阴性的患者。1病例资料患者,男,27岁,因“肢体疼痛无力1月余,加重2周”于2019年6月8日就诊于我科。患者于入院1月前无明显诱因出现肢体疼痛,腰部疼痛症状明显。3周前出现肢体无力感,伴面部皮疹,鼻翼两侧为著。2周前出现全身浮肿,抬头及转颈无力,双上肢抬起费力,坐起费力,行走约50米后肌肉无力酸痛明显,伴双上肢近端、双侧髋部、双膝关节及前胸部皮疹,伴发热,化验示肌酸激酶7500 U/L,行肌电图示肌源性损害,门诊按“肌病”收至我科。患者自发病以来,饮食睡眠可,二便如常,体重增加约10千克。
