miR-301a的上调通过PTEN/PI3K/AKT信号轴诱导巨噬细胞M1极化促进动脉瘤的进展

目的:研究miR-301a通过PTEN/PI3K/AKT信号轴调节巨噬细胞M1极化加速血管紧张素Ⅱ(AngⅡ)诱导的腹动脉瘤(AAA)进展的机制。方法:体内实验中,8~10周龄ApoE^(-/-)雄性小鼠分为对照组、模型组、miR-301a过表达组,输注生理盐水(saline)为对照组(n=15),通过血管紧张素Ⅱ(AngⅡ)诱导建成AAA模型(n=45);THP-1细胞随机分为对照组、AngⅡ+agomir NC组、AngⅡ+miR-301a agomir组和AngⅡ+miR-301a agomir+LY(LY294002)组;使用生物信息学、ELISA、Western blot、qRT-PCR和双荧光素酶实验研究miR-301a调控巨噬细胞极化促动脉瘤进展的机制。结果:与对照组相比,AAA模型组的miR-301a表达上调,主动脉直径增粗,miR-301a的过表达显著增加了动脉直径;TargetScan7.2数据库预测miR-301a与PTEN 3’-UTR之间存在结合位点,双荧光素酶报告基因分析证实PTEN是THP-1巨噬细胞中miR-301a的直接靶标;GO和KEGG通路富集分析显示PI3K/AKT/和NF-κB信号的通路可能是巨噬细胞向M1极化的关键通路;体内、体外实验证明miR-301a通过PI3K/AKT/NF-κB信号通路调控巨噬细胞向M1表型极化,上调基质金属蛋白酶和炎症因子的表达,促进动脉瘤进展。此外,miR-301a联合MMP-9可作为预测动脉瘤破裂的非侵入性生物标志物。结论:miR-301a通过PTEN/PI3K/AKT途径促进巨噬细胞的M1极化,从而加速动脉瘤的进展,在AAA的疾病进展中发挥了关键作用。

Technetium-99m-methylene diphosphonate single photon emission computed tomography/computed tomography combined with prostate-specific antigen/free prostate-specific antigen ratio for bone metastasis of prostate cancer

BACKGROUND Prostate cancer(PC)is one of the most common malignant tumors in men,and bone metastasis is one of its common complications,which seriously affects the quality of life and prognosis of patients.AIM To investigate the diagnostic value of technetium-99m-methylene diphosphonate(99mTc-MDP)single photon emission computed tomography(SPECT)/CT imaging combined with the serum prostate-specific antigen(PSA)/free PSA ratio for PC bone metastasis(PCBM).METHODS One hundred patients with PC who visited the Hospital of Chengdu University of Traditional Chinese Medicine from January 2020 to January 2022 were recruited as the experimental(Exp)group,while 30 patients with benign prostatic lesions(BPLs)were recruited as the control(Ctrl)group.All patients underwent 99mTc-MDP SPECT/CT imaging and serum PSA/fPSA testing.The SPECT/CT imaging results and serum PSA/fPSA ratios of patients were analyzed to evaluate their diagnostic values for PCBM.RESULTS The difference in general information of the patients was not obvious,showing comparability.The two methods showed no visible differences in negative predictive value and sensitivity for patients with PCBM,but had great differences in positive predictive value and specificity(P<0.05).The PSA/fPSA ratio of patients with PC in the Exp group was lower than those with BPLs,and patients with PCBM had a much lower PSA/fPSA ratio than those without PC(P<0.05).The results confirmed that the combined use of 99mTc-MDP SPECT/CT imaging and serum PSA/fPSA ratio achieved a detection rate of 95%for PCBM.CONCLUSION The combination of 99mTc-MDP SPECT/CT and PSA/fPSA ratio is accurate and reliable for the diagnosis of PCBM,which provides an important reference for clinical practice.

Integrin αvβ6 and matrix metalloproteinase 9 correlate with survival in gastric cancer

AIM: To investigate the expression of integrin αvβ6 and matrix metalloproteinase 9 (MMP-9), their association with prognostic factors and to assess their predictive role in gastric cancer patients.METHODS: Immunohistochemistry was used to determine the expressions of integrin αvβ6 and MMP-9 in 126 specimens from patients with primary gastric carcinoma. Associations between immunohistochemical staining and various clinic pathologic variables of tissue specimens were evaluated by the χ2 test and Fisher’s exact test. Expression correlation of αvβ6 and MMP-9 was assessed using bivariate correlation analysis. The patients were followed-up every 3 mo in the first two years and at least every 6 mo afterwards, with a median follow-up of 56 mo (ranging from 2 mo to 94 mo). Four different combinations of αvβ6 and MMP-9 levels (that is, both markers positive, both markers negative, αvβ6 positive with MMP-9 negative, and αvβ6 negative with MMP-9 positive) were evaluated for their relative effect on survival. The difference in survival curves was evaluated with a log-rank test. Survival analysis was conducted using the Kaplan-Meier survival and Cox proportional hazards model analysis.RESULTS: The expressions of integrin αvβ6 and MMP-9 were investigated in 126 cases, among which 34.92% were positive for αvβ6 expression, and 42.06% for MMP-9 expression. The expression of αvβ6 was associated with Lauren type, differentiation, N stage, and TNM stage (the P values were 0.006, 0.038, 0.016, and 0.002, respectively). While MMP-9 expression was associated with differentiation, T stage, N stage, and TNM stage (the P values were 0.039, 0.014, 0.033, and 0.008, respectively). The positive correlation between αvβ6 and MMP-9 in gastric cancer was confirmed by a correlation analysis. The Kaplan-Meier survival analysis showed that patients with expression of αvβ6 or MMP-9 alone died earlier than those with negative expression and that patients who were both αvβ6 and MMP-9 positive had a shorter overall survival than those with the opposite pattern (both αvβ6 and MMP-9 negative) (P = 0.000). A Cox model indicated that positive expression of αvβ6 and MMP-9, diffuse Lauren type, as well as a senior grade of N stage, M stage, and TNM stage were predictors of a poor prognosis in univariate analysis. Only αvβ6 and MMP-9 retained their significance when adjustments were made for other known prognostic factors in multivariate analysis (RR = 2.632, P = 0.003 and RR = 1.813, P = 0.007).CONCLUSION: The expression of αvβ6 and MMP-9 are closely correlated, and the combinational pattern of αvβ6 and MMP-9 can serve as a more effective prognostic index for gastric cancer patients.

Combined Detection of Serum Matrix Metalloproteinase 9,Acetyl Heparinase and Cathepsin L in Diagnosis of Ovarian Cancer

Objective： To investigate the clinic values of combining test of serum matrix metalloproteinase 9 （MMP-9）, acetyl heparinase （Hpa） and Cathepsin L （CL） in diagnosis of ovarian cancer. Methods： Serum levels of MMP-9, Hpa and CL were detected in a total of 418 cases, including 217 cases with ovarian malignant tumor, 100 cases with ovarian benign tumor and 101 healthy controls, by using enzyme-linked immunosorbent assay （ELISA）. Their correlation with clinicopathologic feature of ovarian malignant tumor was analyzed and their diagnosis performance was evaluated by receiver operating characteristic （ROC）. The combined diagnosis model was established by logistic regression analysis. Results： The serum levels of MMP-9, Hpa and CL were significantly higher in patients with ovarian malignant tumor than in benign tumor and healthy control, the serum levels of CL and Hpa were higher in epithelial cancer than in non-epithelial tumor, and MMP-9, Hpa and CL were elevated in low grade and advanced stage compared to high grade and early stage. The sensitivity for diagnosis of ovarian malignant tumor from high to low was CL, Hpa and MMP-9, and the specificity was MMP-9, CL and Hpa. The united diagnosis model was established and showed the sensitivity and specificity of combined detection were 84.6% and 82.1%, respectively, which were significantly higher than a single tumor marker. Conclusion： Serum MMP-9, Hpa and CL were correlated with ovarian malignant tumor and the combined detection of which may be valuable for clinical diagnosis of ovarian malignant tumor.

Matrix metalloproteinase 9 level as an indicator for restenosis following cervical and intracranial angioplasty and stenting

Cervical and intracranial angioplasty and stenting is an effective and safe method of reducing the risk of ischemic stroke, but it may be affected by in-stent restenosis. The present study in-vestigated serum level of matrix metalloproteinase 9 as a predictor of restenosis after 40 patients underwent cervical and/or intracranial angioplasty and stenting. Results showed that resteno-sis occurred in 30% （3/10） of patients when the serum level of matrix metalloproteinase 9 at 3 days after surgery was 2.5 times higher than preoperative level. No restenosis occurred when the serum level of matrix metalloproteinase 9 at 3 days after surgery was not 2.5 times higher than preoperative level. Restenosis occurred in 12% （2/17） of patients when the serum level of matrix metalloproteinase 9 was higher than preoperative level for more than 30 days after surgery, but only occurred in 4% （1/23） of patients when the serum level of matrix metalloproteinase 9 was higher than preoperative level for less than 30 days after surgery. However, the differences observed were not statistically signiifcant （P 〉 0.05）. Experimental ifndings indicate that when the serum level of matrix metalloproteinase 9 is 2.5 times higher than preoperative level at 3 days after cervi-cal and intracranial angioplasty and stenting, it may serve as a predictor of in-stent restenosis.

Immunohistochemical Studies of the Expression of Matrix Metalloproteinase-2 and Metalloproteinase-9 in Human Prostate Cancer

To study the expression of matrix metalloproteinase 2 and 9 in human prostate cancer, matrix metalloproteinase 2 and 9 were immunohistochemically detected in tissues of prostate cancer and benign prostatic hyperplasia (BPH). Our results showed that matrix metalloproteinase 2 and 9 levels in prostate cancer were much higher than those in tissues of BPH, with the cancer invasion being positively correlated with the expression of the metalloproteinases. It is concluded that matrix metalloproteinase 2 and 9 are better molecular markers, which are of help in the diagnosis and prediction of prognosis of prostate cancer.

Diabetes-related intestinal region-specific thickening of ganglionic basement membrane and regionally decreased matrix metalloproteinase 9 expression in myenteric ganglia

BACKGROUND The importance of the neuronal microenvironment has been recently highlighted in gut region-specific diabetic enteric neuropathy. Regionally distinct thickening of endothelial basement membrane(BM) of intestinal capillaries supplying the myenteric ganglia coincide with neuronal damage in different intestinal segments. Accelerated synthesis of matrix molecules and reduced degradation of matrix components may also contribute to the imbalance of extracellular matrix dynamics resulting in BM thickening. Among the matrix degrading proteinases, matrix metalloproteinase 9(MMP9) and its tissue inhibitor(TIMP1) are essential in regulating extracellular matrix remodelling.AIM To evaluate the intestinal segment-specific effects of diabetes and insulin replacement on ganglionic BM thickness, MMP9 and TIMP1 expression.METHODS Ten weeks after the onset of hyperglycaemia gut segments were taken from the duodenum and ileum of streptozotocin-induced diabetic, insulin-treated diabetic and sex-and age-matched control rats. The thickness of BM surrounding myenteric ganglia was measured by electron microscopic morphometry. Wholemount preparations of myenteric plexus were prepared from the different gut regions for MMP9/TIMP1 double-labelling fluorescent immunohistochemistry. Post-embedding immunogold electron microscopy was applied on ultrathin sections to evaluate the MMP9 and TIMP1 expression in myenteric ganglia and their microenvironment from different gut segments and conditions. The MMP9 and TIMP1 messenger ribonucleic acid(m RNA) level was measured by quantitative polymerase chain reaction.RESULTS Ten weeks after the onset of hyperglycaemia, the ganglionic BM was significantly thickened in the diabetic ileum, while it remained intact in the duodenum. The immediate insulin treatment prevented the diabetes-related thickening of the BM surrounding the ileal myenteric ganglia. Quantification of particle density showed an increasing tendency for MMP9 and a decreasing tendency for TIMP1 from the proximal to the distal small intestine under control conditions. In the diabetic ileum, the number of MMP9-indicating gold particles decreased in myenteric ganglia, endothelial cells of capillaries and intestinal smooth muscle cells, however, it remained unchanged in all duodenal compartments. The MMP9/TIMP1 ratio was also decreased in ileal ganglia only. However, a marked segment-specific induction was revealed in MMP9 and TIMP1 at the m RNA levels.CONCLUSION These findings support that the regional decrease in MMP9 expression in myenteric ganglia and their microenvironment may contribute to extracellular matrix accumulation, resulting in a region-specific thickening of ganglionic BM.

TBM患者外周血CD64、miR-155、MMP-9水平变化及对抗结核治疗的指导意义

目的探究结核性脑膜炎(TBM)患者外周血CD64、miR-155、基质金属蛋白酶-9(MMP-9)水平变化及对抗结核治疗的指导意义。方法选取2017年5月至2021年4月昆明市第三人民医院80例TBM患者作为观察组,另选取同期在本院进行脑脊液检查并经影像学、组织学技术确诊的非中枢神经系统疾病患者80例作为对照组,比较两组外周血CD64、miR-155、MMP-9水平及脑脊液生化指标糖、蛋白、氯化物,分析外周血指标与脑脊液指标相关性、外周血CD64、miR-155、MMP-9水平与TBM的关系,比较不同疗效患者外周血CD64、miR-155、MMP-9水平,评价外周血CD64、miR-155、MMP-9水平对TBM患者抗结核治疗疗效的预测价值。结果观察组外周血CD64、miR-155、MMP-9水平及脑脊液蛋白水平高于对照组,脑脊液糖、氯化物水平低于对照组,差异有统计学意义(P<0.05);TBM患者外周血CD64、miR-155、MMP-9水平与脑脊液生化指标蛋白呈正相关关系,与糖、氯化物呈负相关关系(P<0.05);外周血CD64、miR-155、MMP-9水平均为TBM发病独立影响因素(P<0.05);TBM患者中抗结核治疗好转者外周血CD64、miR-155、MMP-9水平低于恶化者,差异有统计学意义(P<0.05);ROC曲线,结果显示外周血CD64、miR-155、MMP-9水平预测TBM患者抗结核治疗恶化的AUC分别为0.756、0.783、0.765,应用Logistic二元回归拟合,logit(p)=4.217+3.124×CD64-3.026×miR-155+3.293×MMP-9,构建各指标联合诊断的AUC为0.902。结论TBM患者外周血CD64、miR-155、MMP-9水平均明显升高,且其水平与疾病发病及抗结核治疗效果有关,临床可检测其水平,用于TBM诊断及治疗方案制定,值得推广与应用。

Role of lymphotoxin alpha as a new molecular biomarker in revolutionizing tear diagnostic testing for dry eye disease

Dry eye disease(DED),primarily classified as multifactorial ocular surface disorder,afflicts tens of millions of individuals worldwide,adversely impacting their quality of life.Extensive research has been conducted on tear film analysis over the past decades,offering a range of tests to evaluate its volume,health,and integrity.Yet,early diagnosis and effective treatment for DED continue to pose significant challenges in clinical settings.Nevertheless,by recognizing key phenomena in DED such as ocular surface inflammation,hyperosmolarity,and tear film instability,this article provides a comprehensive overview of both traditional and recently developed methods for diagnosing and monitoring DED.The information serves as a valuable resource not only for clinical diagnosis but also for further research into DED.

Electroacupuncture ameliorates blood-brain barrier disruption after ischemic stroke through histone acetylation regulation at the matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 2 genes

OBJECTIVE:To explore whether the regulation of matrix metalloproteinase 9(MMP-9)/tissue inhibitors of MMPs(TIMPs)gene expression through histone acetylation is a possible mechanism by which electroacupuncture(EA)protects blood-brain barrier(BBB)integrity in a middle cerebral artery occlusion(MCAO)rat model.METHODS:Male Sprague-Dawley rats were divided into four groups:the sham group,the MCAO group,the MCAO+EA(MEA)group,and the MCAO+EA+HAT inhibitor(HATi)group.The MCAO model was generated by blocking the middle cerebral artery.EA was applied to Baihui(GV20).Samples were collected 1 or 3 d after reperfusion.Neurological function scores and Evans blue extravasation were employed to evaluate the poststroke injury.The effect of EA on MMP-9/TIMPs gene expression was assessed by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)and chromatin immunoprecipitation(ChIP).RESULTS:Our results showed that EA treatment prominently improved neurological function and ameliorated BBB disruption.The RT-qPCR assay showed that EA reduced the expression of MMP-9 and promoted TIMP-2 mRNA expression,but HATi reversed these effects of EA.In addition,ChIP results revealed that EA decreased the enrichment of H3K9ace/H3K27ace at MMP-9 promoters and notably stimulated the recruitment of H3K9ace/H3K27ace at TIMP-2 promoter.CONCLUSION:EA treatment at Baihui(GV20)regulates the transcription of MMP-9 and TIMP-2 through histone acetylation modification in the acute stage of stroke,which preserves the structural integrity of the BBB in MCAO rats.These findings suggested that the histone acetylation-mediated transcriptional activity of target genes may be a crucial mechanism of EA treatment in stroke.

肺癌患者血清MMP-9和TIMP-1检测的临床意义

目的研究基质金属蛋白酶-9(MMP-9)和组织金属蛋白酶抑制剂-1(TIMP-1)在肺癌患者和健康人血清中的含量,及其与分期、肿瘤分化程度、肿瘤大小、淋巴结转移状态、远处转移等病理特征的相关性。方法通过酶联免疫吸附测定(ELISA)分别检测肺癌患者和健康对照者的血清样本中的MMP-9、TIMP-1的浓度,进行统计学分析。结果 MMP-9和TIMP-1在肺癌患者血清中的含量明显高于健康人群,差异有统计学意义(P<0.01);并与分期、肿瘤大小、淋巴结转移、远处转移呈正相关(P<0.05),与分化程度呈负相关(P<0.05);而与年龄、性别、吸烟史、肿瘤的病理类型无关(P>0.05)。结论 MMP-9和TIMP-1参与肺癌的发生、发展、侵袭转移过程,可作为临床监测病情发展的指标。

NF-κB p65对子宫内膜异位症腺上皮细胞OPN、MMP-9表达及细胞侵袭性的影响

目的:探讨RNAi抑制核因子NF-κB p65对子宫内膜异位症(EMs)在位内膜腺上皮细胞中OPN、MMP-9表达及细胞侵袭性的影响。方法:采用NF-κB p65 siRNA基因沉默转染10例EMs患者在位内膜原代腺上皮细胞,由Western blot、Real-time PCR、Transwell等分别检测干扰前后OPN、NF-κB p65、MMP-9蛋白和mRNA表达及细胞侵袭性的变化。结果:与未干预组比较,NF-κB p65 siRNA干预后EMs腺上皮细胞中OPN蛋白及mRNA表达无明显变化,差异无统计学意义(P均>0.05);NF-κB p65、MMP-9蛋白及mRNA表达降低,差异均有统计学意义(P均<0.05)。NF-κB p65 siRNA干预后,EMs腺上皮细胞的细胞侵袭性明显降低,差异有统计学意义(P<0.05)。结论:揭示NF-κB可能通过下调MMP-9的表达进而改变细胞侵袭性诱导EMs的发生。

慢性阻塞性肺病患者血清IL—8和MMP—9检测及其临床意义

目的：探讨慢性阻塞性肺病(COPD)患者急性发作期和缓解期血清IL-8和MMP-9变化的临床意义。方法：采用ELISA方法,分剐检测23例患者在COPD急性发作期、缓解期及20例正常人血清IL-8和MMP-9水平,并进行比较。结果：COPD急性发作期IL-8水平(197.27±65.44)pg·ml^(-1)明显高于缓解期(39.94±9.55)pg·ml^(-1)(P＜0.01),两者间血清MMP-9水平则无显著差异(P＞0.05)。COPD急性发作期和缓解期血清MMP-9水平分别为(206.10±50.88)ng·ml^(-1)和(190.09±43.22)ng·ml^(-1)均明显高于正常人(150.04±61.50)ng·ml^(-1)(P＜0.01)。结论：IL-8参与了COPD的炎症过程,其血清水平有助对COPD急性发作进行监测。MMP-9在COPD急性发作期和缓解期都有高水平表达,可能与气道重塑、肺气肿形成有关。

VEGF与MMP-9在胃癌组织中的表达及相互关系的研究

目的 探讨血管内皮生长因子 (VEGF)和基质金属蛋白酶 9(MMP 9)在胃癌组织中的表达与胃癌预后的关系。方法 免疫组化染色法检测了 4 0例胃癌术后标本中VEGF及以原位杂交技术检测MMP 9mRNA的表达。结果 VEGF、MMP 9mRNA在胃癌组织中的阳性表达率分别为 5 5 %、6 0 % ;VEGF蛋白表达与TNM分期、淋巴结转移关系密切 ;MMP 9mRNA的表达与胃癌的浸润程度、TNM分期、分化程度、淋巴结转移有关 (P <0 .0 5 ) ,而与性别、肿瘤大小、部位无关。结论 MMP 9、VEGF在肿瘤侵蚀转移中起重要作用 ;其共位表达有助于判断胃癌预后。

超微加味丹参饮对原发性高脂血症合并颈动脉粥样硬化患者血清VEGF、MMP-9的影响

目的:观察超微加味丹参饮治疗原发性高脂血症合并颈动脉粥样硬化斑块的临床疗效。方法:60例患者随机分为治疗组(超微加味丹参饮)和对照组(辛伐他汀+阿司匹林),连续治疗6月。观察治疗前后血脂、超敏C反应蛋白(hs-CRP)、血管内皮细胞生长因子(VEGF)、基质金属蛋白酶9(MMP-9)、颈动脉内膜中层厚度(IMT)、颈动脉内膜斑块面积的变化并比较不良反应。结果:经治疗两组血清血脂、hs-CRP、VEGF、MMP-9水平及颈动脉IMT及内膜斑块面积均有明显改善(P<0.05或P<0.01);与对照组比较,超微加味丹参饮在降低血清TC、APOB及VEGF、MMP-9水平,升高HDLC及降低颈动脉IMT,减少颈动脉内膜斑块面积方面疗效更优,两者差异有统计学意义(P<0.05),而对hs-CRP的作用相当(P>0.05)。两组均未出现不良反应及毒副作用。结论:超微加味丹参饮通过调节血脂、降低hs-CRP抑制炎症,下调血清VEGF、MMP-9稳定斑块,降低IMT值,减少颈动脉内膜斑块面积发挥抗AS作用,对原发性高脂血症合并颈动脉粥样硬化斑块有较好的疗效,且临床未见明显毒副作用,优于常规西药治疗。

KISS-1和MMP-9在51例鼻咽鳞状细胞癌中的表达和临床意义

目的探究肿瘤抑制性因子KISS-1及基质金属蛋白酶9(MMP-9)在鼻咽癌发生、发展中的作用及二者之间的关系。方法选择1999年1月至2006年3月在唐山市人民医院初次诊断并手术切除的鼻咽癌51例为鼻咽癌组,鼻咽部慢性炎症组织标本30例为对照组。分别进行HE染色及链霉素抗生物素蛋白-过氧化酶(SP)法进行免疫组织化学染色,检测KISS-1、MMP-9在鼻咽癌和慢性炎症组织中的阳性表达率,并做相关的统计学分析。结果炎性鼻咽组织中KISS-1蛋白的阳性表达率(96.7%)高于鼻咽癌组织中KISS-1蛋白的阳性的表达率(47.1%);慢性鼻咽黏膜炎症组织中MMP-9蛋白呈阴性或低阳性表达(40.0%),而鼻咽鳞状细胞癌中MMP-9蛋白多为高阳性表达(86.3%)。在鼻咽癌组中,KISS-1显现低表达状态,而MMP-9呈高表达状态。统计分析二者成反向表达。结论 KISS-1蛋白表达降低、MMP-9表达升高将有助于鼻咽癌的转移和扩散,同时检测二者的表达状况可指导临床及时有效地采取相应的检查和治疗,为评判临床预后提供切实可行的观测指标。

环氧化酶-2和基质金属蛋白酶-9在骨肉瘤组织中的表达及其临床意义

目的观察环氧化酶-2(Cox-2)和基质金属蛋白酶-9(MMP-9)在骨肉瘤组织中的表达及其临床意义。方法应用免疫组织化学(SP)法,检测35例骨肉瘤、14例骨软骨瘤中Cox-2和MMP-9蛋白的表达情况,并分析其在骨肉瘤不同病理分级、临床分期中的表达及两者之间的相互关系。结果 Cox-2蛋白染色主要表达于细胞浆内,在骨肉瘤中阳性表达率高于在骨软骨瘤中阳性表达率(P<0.05)。MMP-9蛋白染色主要表达于细胞膜和(或)细胞浆内,在骨肉瘤中阳性表达率高于在骨软骨瘤中阳性表达率(P<0.05)。Cox-2和MMP-9蛋白在骨肉瘤组织中的表达与Enneking分期密切相关。Enneking分期Ⅲ期骨肉瘤的Cox-2和MMP-9蛋白的表达强度与Ⅱa期、Ⅱb期比较差异有统计学意义(P<0.05),而Ⅱa期与Ⅱb期的表达强度比较差异无统计学意义(P>0.05)。35例骨肉瘤标本中有23例(65.7%)Cox-2和MMP-9蛋白的表达同时呈阳性,4例(11.4%)同时呈阴性;8例(22.8%)Cox-2和MMP-9蛋白的表达呈不一致性。Cox-2和MMP-9蛋白在骨肉瘤组织中的表达呈正相关。结论 Cox-2和MMP-9蛋白在骨肉瘤组织中均为高表达,提示它们可能对骨肉瘤的发生、发展起重要作用。

原发性高血压患者血压变异性和血清高敏C反应蛋白及基质金属蛋白酶-9关系的研究

目的研究原发性高血压(EH)患者血压变异性(BPV)与高敏C反应蛋白(hs-CRP)、基质金属蛋白酶-9(MMP-9)之间的关系。方法收集符合纳入标准的患者资料,分为高血压组(n=117例)和正常血压对照组(n=32例)。对入选的117例EH患者行24 h动态血压监测(ABPM),根据24 h收缩压变异性(24hSBPV)第50百分位数(P50)将其分为高BPV组(57例)和低BPV(60例)组。所有患者均采用胶乳比浊法测定hs-CRP,ELISA法测定MMP-9,比较各组hs-CRP和MMP-9水平。结果 EH组24 h SBPV高于对照组(P<0.01),hs-CRP和MMP-9较血压正常组也显著升高(P<0.01)。EH组中,高BPV组患者hs-CRP和MMP-9显著升高(P<0.01)。24 h SBPV与年龄、高血压病程、LDL-C、hs-CRP及MMP-9呈正相关;多元线性回归显示,hs-CRP、MMP-9、LDL-C进入回归方程。结论 EH患者BPV可能与hs-CRP及MMP-9相关。

外周血游离CD147及其诱导产物基质金属蛋白酶-9对颅脑创伤患者短期预后的评估意义

目的观察颅脑创伤患者外周血游离CD147及其诱导产物基质金属蛋白酶-9(MMP-9)动态变化,并探讨其表达变化与短期预后的关系。方法纳入2014年5月至2016年12月天津医科大学第二医院收治的39例颅脑创伤患者,酶联免疫吸附试验测定外周血游离CD147和MMP-9表达变化,明胶酶谱法测定MMP-9活性,出院时采用Glasgow预后分级(GOS)评价预后。结果男性与女性患者出院时GOS评分差异无统计学意义(2.63±1.28对2.57±1.14;t=0.161,P=0.873);不同年龄段出院时GOS评分差异具有统计学意义(t=2.191,P=0.038),>60岁患者GOS评分低于<40岁(t=2.645,P=0.014)和40~60岁(t=2.320,P=0.029)患者。根据GOS评分分为预后良好组(GOS评分≥3,25例)和预后不良组(GOS评分<3,14例),预后不良组患者外周血游离CD147[(5.07±1.89)ng/ml对(10.37±1.69)ng/ml;t=2.080,P=0.048]和MMP-9[(41.55±4.67)ng/ml对(75.23±5.18)ng/ml;t=2.512,P=0.019]水平高于预后良好组。偏相关分析结果显示,出院时GOS评分与外周血游离CD147(r=-0.473,P=0.000)和MMP-9(r=-0.435,P=0.036)水平呈负相关。结论颅脑创伤患者外周血游离CD147及其诱导产物MMP-9水平越高、预后越差,可以作为提示颅脑创伤患者预后不良的重要指标。

Relationship between Eukaryotic Translation Initiation Factor 4E and Malignant Angiogenesis in Non-Hodgkin Lymphoma

The relationship between angiogenesis and eukaryotic translation initiation factor 4E （EIF4E） expression level in non Hodgkin lymphoma （NHL） was studied. Mean microvessel density （MVD） and EIF4E were detected in 52 lymph node samples paraffin sections of patients with newly diagnosed NHL by the way of immunohistochemistry. Antisense EIF4E cDNA was cloned into plasmid pcDNA3.1 （＋） and transfected into Raji cells. A series of angiogenesis related factors,including vascular endothelial growth factor （VEGF）, matrix metalloproteinases 9 （MMP-9） and tissue inhibitor of metalloproteinases-2 （TIMP-2） proteins were detected by Western blot. The results showed that： （1） The Expression of EIF4E and MVD was higher in aggressive lymphomas than in indolent lymphomas（P〈0.05）and the expression of EIF4E was positively correlated with MVD in lymph node of NHL（r=0. 695, P〈0.01）. （2） Antisense EIF4E eukaryocytic expression vector （pcDNA3. 1-EIF4Eas） was constructed successfully. （3） EIF4E, VEGF and MMP-9 were expressed at high levels in Raji cells as compared to normal human peripheral blood monocular cells （NHPMC）, and blockage of EIF4E expression brought down the expression of VEGF and MMP-9. However, TIMP-2 was undetectable in Rail cells, although a moderate level of TIMP-2 was detected in NHPMC. It was concluded that the increased EIF4E expression was associated with aggressive property of NHL.