Triacylglycerols(TGs)are the main constituents(95%-98%)of plant oil with high nutritional value.They are usually determined by high performance liquid chromatography coupled with mass spectrometry(HPLC-MS)which takes ...Triacylglycerols(TGs)are the main constituents(95%-98%)of plant oil with high nutritional value.They are usually determined by high performance liquid chromatography coupled with mass spectrometry(HPLC-MS)which takes a relatively long time during chromatographic separation.Therefore,in this study,a method about fast determination and quantification of TGs in plant oil based on MALDI-TOF-MS(matrix-assisted laser desorption ionization time-of-flight mass spectrometry)was established.OCNT(oxidized carbon nanotube)and CNT(carbon nanotube)were firstly used as matrix of MALDI-TOF-MS for TGs identification.Satisfyingly,OCNT showed good ionization efficiency,especially when used with CHCA(α-Cyano-4-hydroxy cinnamic acid).Sandwich method was optimized to solve the problem that how matrix and sample were deposited to the plate well and to improve ionization efficiency.Nine calibration curves were established for TGs quantification analysis in plant oil.To verify the accuracy of this method,relative content(%)of fatty acids(FAs)calculated from TGs in peanut oil,rapeseed oil,and soybean oil,respectively,by MALDI-TOF-MS were compared with that calculated from FAMEs(fatty acid methyl esters)by gas chromatography.In general,the results were relatively consistent,proving that this method was convenient,fast and accurate for the determination of main TG molecular species in plant oil.展开更多
The population dynamic tendency of Adenophora Iobophvlla Hong as an endangered species and Adenophora potaninii Korsh as widespread species. has been predicted by the Leslie matrix. And the comparison and analysis on ...The population dynamic tendency of Adenophora Iobophvlla Hong as an endangered species and Adenophora potaninii Korsh as widespread species. has been predicted by the Leslie matrix. And the comparison and analysis on the age structures between two species have been carried out in this paper. The results demonstrate the A. lobophylla popuations which have the reasonable age struetures perform slowly negative or positive increment at altitude 2300-3400 m. Especially. below altitude 2700 m. there are many populations performing seriously declining tendcncy. Contrary, A potaninii populations could adapt to environment perfectly at the corresponding condition without finding the population which performs the seriously declining tendency. The differences in developing tendency of population between the two species demonstrate that A. Iobopliylla populations have the weaker ability to adapt to the external unfavorable conditions.展开更多
基金supported by the National Natural Science Foundation of China (31571926)the Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences (CAASASTIP-2013-OCRI)the Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education) of Department of Chemistry of Wuhan University
文摘Triacylglycerols(TGs)are the main constituents(95%-98%)of plant oil with high nutritional value.They are usually determined by high performance liquid chromatography coupled with mass spectrometry(HPLC-MS)which takes a relatively long time during chromatographic separation.Therefore,in this study,a method about fast determination and quantification of TGs in plant oil based on MALDI-TOF-MS(matrix-assisted laser desorption ionization time-of-flight mass spectrometry)was established.OCNT(oxidized carbon nanotube)and CNT(carbon nanotube)were firstly used as matrix of MALDI-TOF-MS for TGs identification.Satisfyingly,OCNT showed good ionization efficiency,especially when used with CHCA(α-Cyano-4-hydroxy cinnamic acid).Sandwich method was optimized to solve the problem that how matrix and sample were deposited to the plate well and to improve ionization efficiency.Nine calibration curves were established for TGs quantification analysis in plant oil.To verify the accuracy of this method,relative content(%)of fatty acids(FAs)calculated from TGs in peanut oil,rapeseed oil,and soybean oil,respectively,by MALDI-TOF-MS were compared with that calculated from FAMEs(fatty acid methyl esters)by gas chromatography.In general,the results were relatively consistent,proving that this method was convenient,fast and accurate for the determination of main TG molecular species in plant oil.
文摘The population dynamic tendency of Adenophora Iobophvlla Hong as an endangered species and Adenophora potaninii Korsh as widespread species. has been predicted by the Leslie matrix. And the comparison and analysis on the age structures between two species have been carried out in this paper. The results demonstrate the A. lobophylla popuations which have the reasonable age struetures perform slowly negative or positive increment at altitude 2300-3400 m. Especially. below altitude 2700 m. there are many populations performing seriously declining tendcncy. Contrary, A potaninii populations could adapt to environment perfectly at the corresponding condition without finding the population which performs the seriously declining tendency. The differences in developing tendency of population between the two species demonstrate that A. Iobopliylla populations have the weaker ability to adapt to the external unfavorable conditions.
