The Role of Vacuolar Processing Enzyme

The classification, characters and maturation methods of VPEs were sum- marized, and its regulation function to vacuolar was also analyzed. Furthermore, effects of the enzyme in vacuolar-mediated plant defense mechanism were discussed to point out that VPEs were divided into 3 subfamilies via autocatalytic mature including seed-type VPE, vegetative-type VPE and new-type VPE. Especially, seed- type VPE mediated the process of storage protein, while vegetative-type VPE and new-type VPE regulated and controlled programmed death of plant cells.

Establishment of a comprehensive indicator to nondestructively analyze watermelon quality at different ripening stages

Two nondestructive methods based on visible and near-infrared(VIS-NIR)spectroscopy and X-ray image have been used for the evaluation of watermelon quality.The prediction perform-ance based on partial least squares(PLS)by diffuse transmittance measurement(500-1010 nm)was evaluated for_chemical quality attributes SSC(Rc=0.903;RMSEC=0.572%Brix;Rp=0.862;RMSEP=0.717%Brix;RPD=1.83),lycopene(Rc=0.845;RMSEC=0.266 mg/100 gFW;Rp=0.751;RMSEP=0.439 mg/100 gFW;RPD=1.13)and moisture(Rc=0.917;RMSEC=0.280%;Rp=0.937;RMSEP=0.276%;RPD=2.79).The X-ray calibration linearequations developed by extracting the appropriate gray threshold were sufficiently precise forvolume(R?=0.986)and weight(R?=0.993).In order to optimize prediction model of water-melon quality in growth period,multivariate multi-block technique factor analysis enabled in-tegration of these traits:chemical information is related to physical infomation.Applyingprinciple component analysis to extract common factors and varimax with Kaiser normalizationto improve explanatory,the comprehensive indicator based on variances was established satis.factorily with Rc=0.94,RMSEC=0.244,Rp=0.93,RMSEP=0.344 and RPD=2.00.Acomparison of these models indicates that the comprehensive indicator determined only by portable VIS-NIR spectrometer appears as a suitable method for appraising watermelon qualitynondestructively on the plant at diferent ripen stages.This method contributes to infer the picking date of watermelon with higher accuracy and bigger economic benefits than that byexperience.

Key Techniques for the Development of Web-Based PDM System

Some key techniques for the development of web-based product data management （PDM） system are introduced. The four-tiered B/S architecture of a PDM system-BITPDM is introduced first, followed by its design and implementation, including virtual data vault, flexible coding system, document management, product structure and configuration management, workflow/process and product maturity management. BITPDM can facilitate the activities from new product introduction phase to manufacturing, and manage the product data andtheir dynamic changing history. Based on Microsoft. NET, XML, web service and SOAP techniques, BITPDM realizes the integration and efficient management of product information.

Impaired XPO5 function leads to transportation failure of microRNA precursor

MicroRNAs（miRNAs）constitute a class of small non-coding RNAs that participate in various biological processes by repressing protein translation or destabilizing target mRNAs.miRNA biogenesis begins with the transcription of primary miRNAs（pri-miRNAs）in the cell nucleus,which are then cleaved by the Drosha/DGCR complex into precursor miRNAs（pre-miRNAs）.The pre-miRNAs are transported by exportin-5（XPO5）from the nucleus to the cytoplasm,