[Objective] The aim of this study is to understand the population composition of methanogens in rumen fluid of grazing Inner Mongolian cashmere goat. [Method] Total DNAs of various bacteria in rumen fluid were isolate...[Objective] The aim of this study is to understand the population composition of methanogens in rumen fluid of grazing Inner Mongolian cashmere goat. [Method] Total DNAs of various bacteria in rumen fluid were isolated for PCR amplification using the specifically designed primers based on conservative mcrA sequence of methanogens; then mcrA specific clone library was accordingly established. The restriction fragment length polymorphism(RFLP) of the library was further analyzed by digestion of restriction enzyme Taq I. [Result] One hundred and five randomly selected specific colonies were classified into six RFLP types, among which the dominant type accounts for 38%, and other types account for 27%, 18%, 5.5% and 4.5%, respectively. [Conclusion] There are at least six different methanogens in rumen fluid of grazing Inner Mongolian cashmere goat.展开更多
为了解天然放牧和舍饲条件下内蒙古绒山羊瘤胃中甲烷菌的种群组成。选取甲烷菌mcrA基因的特异性引物序列,利用PCR技术对从瘤胃液中抽提的细菌总DNA进行扩增,并建立了甲烷菌特异性的mcrA基因文库。用限制性内切酶Taq I对该文库特异性片...为了解天然放牧和舍饲条件下内蒙古绒山羊瘤胃中甲烷菌的种群组成。选取甲烷菌mcrA基因的特异性引物序列,利用PCR技术对从瘤胃液中抽提的细菌总DNA进行扩增,并建立了甲烷菌特异性的mcrA基因文库。用限制性内切酶Taq I对该文库特异性片段进行了限制性酶切片断长度多态性分析(Restriction fragment lengthpolymor-phism,RFLP)。放牧和舍饲内蒙古绒山羊瘤胃中甲烷菌的mcrA基因片段被分成6个不同的RFLP类型,并由此建立了DNA指纹图谱;系统发育树分析表明,其属于甲烷杆菌目和甲烷微菌目,且绒山羊瘤胃中的甲烷菌大部分属于甲烷短杆菌。放牧绒山羊瘤胃内甲烷菌种类比舍饲绒山羊更加丰富,6种甲烷菌所占比例均较高,而舍饲绒山羊只有两种优势菌比例较高,其他几种所占比例极低。展开更多
文摘[Objective] The aim of this study is to understand the population composition of methanogens in rumen fluid of grazing Inner Mongolian cashmere goat. [Method] Total DNAs of various bacteria in rumen fluid were isolated for PCR amplification using the specifically designed primers based on conservative mcrA sequence of methanogens; then mcrA specific clone library was accordingly established. The restriction fragment length polymorphism(RFLP) of the library was further analyzed by digestion of restriction enzyme Taq I. [Result] One hundred and five randomly selected specific colonies were classified into six RFLP types, among which the dominant type accounts for 38%, and other types account for 27%, 18%, 5.5% and 4.5%, respectively. [Conclusion] There are at least six different methanogens in rumen fluid of grazing Inner Mongolian cashmere goat.
文摘为了解天然放牧和舍饲条件下内蒙古绒山羊瘤胃中甲烷菌的种群组成。选取甲烷菌mcrA基因的特异性引物序列,利用PCR技术对从瘤胃液中抽提的细菌总DNA进行扩增,并建立了甲烷菌特异性的mcrA基因文库。用限制性内切酶Taq I对该文库特异性片段进行了限制性酶切片断长度多态性分析(Restriction fragment lengthpolymor-phism,RFLP)。放牧和舍饲内蒙古绒山羊瘤胃中甲烷菌的mcrA基因片段被分成6个不同的RFLP类型,并由此建立了DNA指纹图谱;系统发育树分析表明,其属于甲烷杆菌目和甲烷微菌目,且绒山羊瘤胃中的甲烷菌大部分属于甲烷短杆菌。放牧绒山羊瘤胃内甲烷菌种类比舍饲绒山羊更加丰富,6种甲烷菌所占比例均较高,而舍饲绒山羊只有两种优势菌比例较高,其他几种所占比例极低。
