Background: The constant interaction between diet and intestinal barrier has a crucial role in determining gut health in pigs. Hermetia illucens(HI) meal(that represents a promising, alternative feed ingredient for pr...Background: The constant interaction between diet and intestinal barrier has a crucial role in determining gut health in pigs. Hermetia illucens(HI) meal(that represents a promising, alternative feed ingredient for production animals) has recently been demonstrated to influence colonic microbiota, bacterial metabolite profile and mucosal immune status of pigs, but no data about modulation of gut mucin dynamics are currently available. The present study evaluated the effects of dietary HI meal inclusion on the small intestinal mucin composition of piglets, as well as providing insights into the cecal microbiota and the mucosal infiltration with immune cells.Results: A total of 48 weaned piglets were randomly allotted to 3 dietary treatments(control diet [C] and 5% or10% HI meal inclusion [HI5 and HI10], with 4 replicate boxes/treatment and 4 animals/box) and slaughtered after61 days of trial(3 animals/box, 12 piglets/diet). The cecal microbiota assessment by 16 S r RNA amplicon based sequencing showed higher beta diversity in the piglets fed the HI-based diets than the C(P < 0.001). Furthermore,the HI-fed animals showed increased abundance of Blautia, Chlamydia, Coprococcus, Eubacterium, Prevotella,Roseburia, unclassified members of Ruminococcaceae, Ruminococcus and Staphylococcus when compared to the C group(FDR < 0.05). The gut of the piglets fed the HI-based diets showed greater neutral mucin percentage than the C(P < 0.05), with the intestinal neutral mucins of the HI-fed animals being also higher than the sialomucins and the sulfomucins found in the gut of the C group(P < 0.05). Furthermore, the piglets fed the HI-based diets displayed lower histological scores in the jejunum than the other gut segments(ileum [HI5] or ileum and duodenum [HI10], P < 0.05).Conclusions: Dietary HI meal utilization positively influenced the cecal microbiota and the small intestinal mucin dynamics of the piglets in terms of selection of potentially beneficial bacteria and preservation of mature mucin secretory architecture, without determining the development of gut inflammation. These findings further confirm the suitability of including insect meal in swine diets.展开更多
A two-step biotechnological process was developed using Bacillus licheniformis S6 to provide a simple and economical procedure which significantly improved feather meal nutrition value. Compared with IFM （initial fea...A two-step biotechnological process was developed using Bacillus licheniformis S6 to provide a simple and economical procedure which significantly improved feather meal nutrition value. Compared with IFM （initial feather meal） and CFM （commercial feather meal）, SFEFM （feather meal gained by solid fermentation and enzymolysis with continuous agitation） had a significant improvement （P〈0.05） in vitro digestibility, contents of oligopeptides and soluble protein released in digestive juice by pepsin- pancreatin digestion procedure, furthermore, some deficient essential amino acids in feather protein （histidine, methionine, lysine） were enhanced. Comapared with CFM, the oligopeptides released into digestive juice of ISFM （feather meal obtained by the biotechnological process described in the paper with intermittent shaking） was significantly enhanced （P〈O.05）, and its in vitro digestibility was statistically （P〉0.05） equivalent to CFM. The summary of the finding to IFM treatment and possible means of further improvements were also listed.展开更多
To reduce the pressure on aquatic resources due to the use of fishmeal in fish feed, the mastery and use of alternative sources of fishmeal in all stages of fish development are essential. Five diets including 4 exper...To reduce the pressure on aquatic resources due to the use of fishmeal in fish feed, the mastery and use of alternative sources of fishmeal in all stages of fish development are essential. Five diets including 4 experimental and one control diet were considered. Experimental diets of post-larvae were developed from unconventional ingredients in which fishmeal was completely replaced by a mixture of brewer’s yeast, chicken viscera and maggots. These foods were tested on Clarias gariepinus post-larvae with an initial average weight of 80 ± 1 mg. 1500 fish individuals were equally distributed in 15 concrete basins with a volume of 500 L forming five treatments in triplicate. Fish individuals were fed, manually at a ration rate of 20%, four times per day. The specific growth rates were 8.38%/day in imported food and 6.35%/day in the experimental food receiving 45.5% of protein from unconventional meal. Similar results were obtained with the feed consumption index. These encouraging results show that it is possible to partially or completely replace fishmeal in catfish feed and obtain an economically profitable production. The recovery of waste such as chicken viscera, brewer’s yeast and soybean meal in fish food formulation as tested in this study is a good contribution to environmental sanitation.展开更多
Background:The relationship between diet and intestinal microbiota and mucin composition appears to be fundamental for poultry gut health.The effects of insect meal(whose role as alternative feed ingredient is now wel...Background:The relationship between diet and intestinal microbiota and mucin composition appears to be fundamental for poultry gut health.The effects of insect meal(whose role as alternative feed ingredient is now well recognized)on gut microbiota and mucin composition have recently been reported in Tenebrio molitor-fed free-range and broiler chickens,but no data are currently available for Hermetia illucens(HI)-fed broilers.The present study evaluated the effects of dietary HI meal inclusion on cecal microbiota and intestinal mucin composition of broiler chickens.Results:A total of 256 male broiler chickens were allotted to 4 dietary treatments(control diet[C]and 5%,10%and 15%HI meal inclusion,with 8 replicate pens/treatment and 8 birds/pen)and slaughtered at 35 d of age(2 animals/pen,16 birds/diet).The cecal microbiota assessment by 16S rRNA amplicon based sequencing showed lower alpha diversity in HI15 chickens(Shannon,P<0.05)and higher beta diversity(Adonis and ANOSIM,P<0.001)in birds fed HI diets than C.Furthermore,HI15 birds displayed significant increase of the relative abundance of Proteobacteria phylum(False Discovery Rate[FDR]<0.05)when compared to HI10.L-Ruminococcus(Ruminococcus from Lachnospiraceae family),Faecalibacterium,Blautia and Clostridium genera were found to be characteristic of HI5 cecal microbiota(FDR<0.05),while broiler chickens fed HI10 and HI15 diets were characterized(FDR<0.05)by Lactobacillus and Ruminococcus(HI10)and Bacteroides,Roseburia and Helicobacter genera(HI15).Periodic-acid Schiff,Alcian Blue pH 2.5 and high iron diamine staining on small and large intestine also demonstrated lower mucin staining intensity in the intestinal villi of HI10 and HI15 birds than C(P<0.05).Conclusions:Dietary HI meal utilization at low inclusion levels(i.e.,5%)positively influenced either the cecal microbiota or the gut mucin dynamics in terms of selection of potentially beneficial bacteria and increase in villi mucins.However,high inclusion levels(in particular the 15%)may have a negative influence in terms of partial reduction of microbial complexity,reduction of potentially beneficial bacteria,selection of bacteria with mucolytic activity and decrease in villi mucins.展开更多
IN Guangzhou, where restaurants are plentiful and food from many places is served, the baozai meal, a kind of traditional Chinese fast food, has retained its place in the hearts of Guangzhou’s caterers. The history o...IN Guangzhou, where restaurants are plentiful and food from many places is served, the baozai meal, a kind of traditional Chinese fast food, has retained its place in the hearts of Guangzhou’s caterers. The history of the baozai meal starts at least from the beginning of this century. By the end of the Qing Dynasty, Guangzhou was the largest trading port in south China with a developed, commodities-based economy. There were many dockworkers and porters living in the city at the time. They worked very hard and were often too busy to eat their meals at home. As a result, different kinds of meals were invented to meet their needs, such as the botou meal (bo is kind of earthen bowl), the dietou meal (die is small plate) and the baozai展开更多
This work aimed to explore an effective way for converting agro-industrial residues into value-added products.A thermophilic protease-producing strain was isolated from soybean meal(SBM)and identified as Bacillus lich...This work aimed to explore an effective way for converting agro-industrial residues into value-added products.A thermophilic protease-producing strain was isolated from soybean meal(SBM)and identified as Bacillus licheniformis by 16S rDNA sequencing.After incubating Bacillus licheniformis CPB2 in casein medium for 24 h,the protease activity,peptide yield,and protein conversion rate of the culture could reach 8.87±0.33 U/mL,68.58%,and 82.27%,respectively.Brewer’s spent grain(BSG)and SBM were used as co-substrates to produce value-added peptides through thermophilic solid-state fermentation(TSSF)with Bacillus licheniformis CPB2.The peptide content of the co-substrates increased from 25.71 mg/g to 112.72 mg/g under the optimal TSSF conditions.The fermented products showed an increased crude protein and free amino acids contents and a decreased crude fat and ash contents as well as trypsin inhibitor activity compared with the unfermented co-substrates.TSSF could degrade macromolecular proteins into peptides,unfold the protein aggregates,and reduce the particle size.Industrial relevance:This work proposed an effective way for the rational and efficient utilization of wet and dry agro-industrial residues to produce value-added products,which showed a broad potential for reducing production costs,saving water resources and energy,and preserving the ecological environment.展开更多
基金provided by Martini Group (Premio Iller Campani)by the University of Turin (ex 60%) grant (Es. fin. 2015–2016–2017)。
文摘Background: The constant interaction between diet and intestinal barrier has a crucial role in determining gut health in pigs. Hermetia illucens(HI) meal(that represents a promising, alternative feed ingredient for production animals) has recently been demonstrated to influence colonic microbiota, bacterial metabolite profile and mucosal immune status of pigs, but no data about modulation of gut mucin dynamics are currently available. The present study evaluated the effects of dietary HI meal inclusion on the small intestinal mucin composition of piglets, as well as providing insights into the cecal microbiota and the mucosal infiltration with immune cells.Results: A total of 48 weaned piglets were randomly allotted to 3 dietary treatments(control diet [C] and 5% or10% HI meal inclusion [HI5 and HI10], with 4 replicate boxes/treatment and 4 animals/box) and slaughtered after61 days of trial(3 animals/box, 12 piglets/diet). The cecal microbiota assessment by 16 S r RNA amplicon based sequencing showed higher beta diversity in the piglets fed the HI-based diets than the C(P < 0.001). Furthermore,the HI-fed animals showed increased abundance of Blautia, Chlamydia, Coprococcus, Eubacterium, Prevotella,Roseburia, unclassified members of Ruminococcaceae, Ruminococcus and Staphylococcus when compared to the C group(FDR < 0.05). The gut of the piglets fed the HI-based diets showed greater neutral mucin percentage than the C(P < 0.05), with the intestinal neutral mucins of the HI-fed animals being also higher than the sialomucins and the sulfomucins found in the gut of the C group(P < 0.05). Furthermore, the piglets fed the HI-based diets displayed lower histological scores in the jejunum than the other gut segments(ileum [HI5] or ileum and duodenum [HI10], P < 0.05).Conclusions: Dietary HI meal utilization positively influenced the cecal microbiota and the small intestinal mucin dynamics of the piglets in terms of selection of potentially beneficial bacteria and preservation of mature mucin secretory architecture, without determining the development of gut inflammation. These findings further confirm the suitability of including insect meal in swine diets.
文摘A two-step biotechnological process was developed using Bacillus licheniformis S6 to provide a simple and economical procedure which significantly improved feather meal nutrition value. Compared with IFM （initial feather meal） and CFM （commercial feather meal）, SFEFM （feather meal gained by solid fermentation and enzymolysis with continuous agitation） had a significant improvement （P〈0.05） in vitro digestibility, contents of oligopeptides and soluble protein released in digestive juice by pepsin- pancreatin digestion procedure, furthermore, some deficient essential amino acids in feather protein （histidine, methionine, lysine） were enhanced. Comapared with CFM, the oligopeptides released into digestive juice of ISFM （feather meal obtained by the biotechnological process described in the paper with intermittent shaking） was significantly enhanced （P〈O.05）, and its in vitro digestibility was statistically （P〉0.05） equivalent to CFM. The summary of the finding to IFM treatment and possible means of further improvements were also listed.
文摘To reduce the pressure on aquatic resources due to the use of fishmeal in fish feed, the mastery and use of alternative sources of fishmeal in all stages of fish development are essential. Five diets including 4 experimental and one control diet were considered. Experimental diets of post-larvae were developed from unconventional ingredients in which fishmeal was completely replaced by a mixture of brewer’s yeast, chicken viscera and maggots. These foods were tested on Clarias gariepinus post-larvae with an initial average weight of 80 ± 1 mg. 1500 fish individuals were equally distributed in 15 concrete basins with a volume of 500 L forming five treatments in triplicate. Fish individuals were fed, manually at a ration rate of 20%, four times per day. The specific growth rates were 8.38%/day in imported food and 6.35%/day in the experimental food receiving 45.5% of protein from unconventional meal. Similar results were obtained with the feed consumption index. These encouraging results show that it is possible to partially or completely replace fishmeal in catfish feed and obtain an economically profitable production. The recovery of waste such as chicken viscera, brewer’s yeast and soybean meal in fish food formulation as tested in this study is a good contribution to environmental sanitation.
基金Financial support for this work was provided by University of Turin(ex 60%)grant(Es.fin.2015-2016-2017).
文摘Background:The relationship between diet and intestinal microbiota and mucin composition appears to be fundamental for poultry gut health.The effects of insect meal(whose role as alternative feed ingredient is now well recognized)on gut microbiota and mucin composition have recently been reported in Tenebrio molitor-fed free-range and broiler chickens,but no data are currently available for Hermetia illucens(HI)-fed broilers.The present study evaluated the effects of dietary HI meal inclusion on cecal microbiota and intestinal mucin composition of broiler chickens.Results:A total of 256 male broiler chickens were allotted to 4 dietary treatments(control diet[C]and 5%,10%and 15%HI meal inclusion,with 8 replicate pens/treatment and 8 birds/pen)and slaughtered at 35 d of age(2 animals/pen,16 birds/diet).The cecal microbiota assessment by 16S rRNA amplicon based sequencing showed lower alpha diversity in HI15 chickens(Shannon,P<0.05)and higher beta diversity(Adonis and ANOSIM,P<0.001)in birds fed HI diets than C.Furthermore,HI15 birds displayed significant increase of the relative abundance of Proteobacteria phylum(False Discovery Rate[FDR]<0.05)when compared to HI10.L-Ruminococcus(Ruminococcus from Lachnospiraceae family),Faecalibacterium,Blautia and Clostridium genera were found to be characteristic of HI5 cecal microbiota(FDR<0.05),while broiler chickens fed HI10 and HI15 diets were characterized(FDR<0.05)by Lactobacillus and Ruminococcus(HI10)and Bacteroides,Roseburia and Helicobacter genera(HI15).Periodic-acid Schiff,Alcian Blue pH 2.5 and high iron diamine staining on small and large intestine also demonstrated lower mucin staining intensity in the intestinal villi of HI10 and HI15 birds than C(P<0.05).Conclusions:Dietary HI meal utilization at low inclusion levels(i.e.,5%)positively influenced either the cecal microbiota or the gut mucin dynamics in terms of selection of potentially beneficial bacteria and increase in villi mucins.However,high inclusion levels(in particular the 15%)may have a negative influence in terms of partial reduction of microbial complexity,reduction of potentially beneficial bacteria,selection of bacteria with mucolytic activity and decrease in villi mucins.
文摘IN Guangzhou, where restaurants are plentiful and food from many places is served, the baozai meal, a kind of traditional Chinese fast food, has retained its place in the hearts of Guangzhou’s caterers. The history of the baozai meal starts at least from the beginning of this century. By the end of the Qing Dynasty, Guangzhou was the largest trading port in south China with a developed, commodities-based economy. There were many dockworkers and porters living in the city at the time. They worked very hard and were often too busy to eat their meals at home. As a result, different kinds of meals were invented to meet their needs, such as the botou meal (bo is kind of earthen bowl), the dietou meal (die is small plate) and the baozai
基金financial support provided by the Key R&D Plan of Jiangsu Province(Key Project of Modern Agriculture,grant BE2016355).
文摘This work aimed to explore an effective way for converting agro-industrial residues into value-added products.A thermophilic protease-producing strain was isolated from soybean meal(SBM)and identified as Bacillus licheniformis by 16S rDNA sequencing.After incubating Bacillus licheniformis CPB2 in casein medium for 24 h,the protease activity,peptide yield,and protein conversion rate of the culture could reach 8.87±0.33 U/mL,68.58%,and 82.27%,respectively.Brewer’s spent grain(BSG)and SBM were used as co-substrates to produce value-added peptides through thermophilic solid-state fermentation(TSSF)with Bacillus licheniformis CPB2.The peptide content of the co-substrates increased from 25.71 mg/g to 112.72 mg/g under the optimal TSSF conditions.The fermented products showed an increased crude protein and free amino acids contents and a decreased crude fat and ash contents as well as trypsin inhibitor activity compared with the unfermented co-substrates.TSSF could degrade macromolecular proteins into peptides,unfold the protein aggregates,and reduce the particle size.Industrial relevance:This work proposed an effective way for the rational and efficient utilization of wet and dry agro-industrial residues to produce value-added products,which showed a broad potential for reducing production costs,saving water resources and energy,and preserving the ecological environment.
