Bronchiectasis is a chronic lung disorder and a number of bacterial pathogens are involved.However,30%-40% of sputum and purulent samples in good quality failed to grow any pathogenic bacteria,making it difficult to c...Bronchiectasis is a chronic lung disorder and a number of bacterial pathogens are involved.However,30%-40% of sputum and purulent samples in good quality failed to grow any pathogenic bacteria,making it difficult to confirm the pathogen.In this study,we collected bronchoalveolar lavage fluid from a bronchiectasis patient undergoing acute exacerbation,and sent for 16 S rDNA pyrosequencing by a 454 GS Junior machine.Metagenomic analysis showed the composition of bacterial community in sample was complex.More than a half of reads（51.3%） were from Pseudomonas aeruginosa.This result was corresponding with the culture result but came out 2 d earlier,which is meaningful for early diagnosis and treatment.The detection with 16 S rDNA pyrosequencing technology is more sensitive and rapid than routine culture,and can detect the co-infection or symbiosis in airway,giving us a novel and convenient approach to perform rapid diagnosis.展开更多
GIVING birth to a child was a life-or-death struggle forthe mother and baby as late as the early half of thiscentury;an estimate survey revealed a mortality rate amongmothers as high as 15 per thousand and infant mort...GIVING birth to a child was a life-or-death struggle forthe mother and baby as late as the early half of thiscentury;an estimate survey revealed a mortality rate amongmothers as high as 15 per thousand and infant mortality as highas 200 per tnousand at that time.Midwifery was oftenpracticed by the mother-in-law,the mother herself,or morepopularly,by an elderly woman in the village who展开更多
基金supported by grants from the Ministry of Science and Technology of China(National High-Tech R&D program:No.2006AA02Z4A9 and No.2012BAI05B0212th Five-Year Plan Major Scientific and Technological Program:No.2012ZX10004-206)the Ministry of Health of China(Special Fund for Health-scientific Research in the Public Interest Program:No.201202011)
文摘Bronchiectasis is a chronic lung disorder and a number of bacterial pathogens are involved.However,30%-40% of sputum and purulent samples in good quality failed to grow any pathogenic bacteria,making it difficult to confirm the pathogen.In this study,we collected bronchoalveolar lavage fluid from a bronchiectasis patient undergoing acute exacerbation,and sent for 16 S rDNA pyrosequencing by a 454 GS Junior machine.Metagenomic analysis showed the composition of bacterial community in sample was complex.More than a half of reads（51.3%） were from Pseudomonas aeruginosa.This result was corresponding with the culture result but came out 2 d earlier,which is meaningful for early diagnosis and treatment.The detection with 16 S rDNA pyrosequencing technology is more sensitive and rapid than routine culture,and can detect the co-infection or symbiosis in airway,giving us a novel and convenient approach to perform rapid diagnosis.
文摘GIVING birth to a child was a life-or-death struggle forthe mother and baby as late as the early half of thiscentury;an estimate survey revealed a mortality rate amongmothers as high as 15 per thousand and infant mortality as highas 200 per tnousand at that time.Midwifery was oftenpracticed by the mother-in-law,the mother herself,or morepopularly,by an elderly woman in the village who
