为了明确绣球〔Hydrangea macrophylla(Thunb.)Ser.〕品种的耐寒性及筛选耐寒性评价主要指标,对40个绣球品种的低温半致死温度(LT_(50))进行比较和耐寒性分级,在此基础上,对5℃、0℃、-5℃、-10℃、-15℃、-20℃下耐寒品种‘小町’(‘Ko...为了明确绣球〔Hydrangea macrophylla(Thunb.)Ser.〕品种的耐寒性及筛选耐寒性评价主要指标,对40个绣球品种的低温半致死温度(LT_(50))进行比较和耐寒性分级,在此基础上,对5℃、0℃、-5℃、-10℃、-15℃、-20℃下耐寒品种‘小町’(‘Komachi’)、‘佳澄’(‘Kasumi’)、‘无尽夏’(‘Endless Summer’)和不耐寒品种‘甜蜜幻想’(‘Sweet Fantasy’)、‘灵感’(‘Inspiration’)、‘你我的情感’(‘You and Me Feelings’)叶的抗氧化酶活性及丙二醛(MDA)和渗透调节物质含量进行比较,并对这些耐寒指标进行相关性分析和主成分分析。结果表明:供试绣球品种LT 50的波动较大,变化范围为-36.68℃~-1.87℃,其中,‘小町’的LT_(50)最低,‘佳澄’、‘平瓣’(‘Flat’)、‘蒂亚娜’(‘Tijana’)、‘塞尔玛’(‘Selma’)、‘含羞叶’(‘Elbtal’)、‘花手鞠’(‘Stockings’)、‘蓝色多瑙河’(‘Blue Danube’)和‘无尽夏’的LT_(50)均低于-20℃。40个绣球品种的耐寒性被分成不耐寒、较耐寒、耐寒和强耐寒4个等级,分别包含13、18、8和1个品种。6个代表品种的过氧化氢酶(CAT)、多酚氧化酶(PPO)和超氧化物歧化酶活性及MDA、脯氨酸(Pro)、可溶性蛋白质(SP)和可溶性糖含量随温度降低基本呈先升高后降低的变化趋势。相关性分析结果表明PPO活性和Pro含量与LT_(50)呈极显著(P<0.01)负相关。主成分分析结果表明:前4个主成分的累计贡献率为81.212%,说明这4个主成分基本能够反映绣球品种的耐寒性;PPO活性、CAT活性、Pro含量和SP含量的负荷量绝对值分别在第1、第2、第3和第4主成分中最高。研究结果显示:供试绣球品种间的耐寒性差异较大,‘小町’等9个品种的耐寒性较强,能够在冬季温度偏低地区露天栽培,并可作为绣球耐寒品种选育的亲本。PPO活性、CAT活性、Pro含量和SP含量为绣球耐寒性评价的主要指标,尤其是PPO活性和Pro含量。展开更多
Total dissolved gas(TDG) supersaturation caused by dam sluicing can result in gas bubble trauma(GBT) in fish and threaten their survival.In the present study,Chinese suckers(Myxocyprinus asiaticus Bleeker) were expose...Total dissolved gas(TDG) supersaturation caused by dam sluicing can result in gas bubble trauma(GBT) in fish and threaten their survival.In the present study,Chinese suckers(Myxocyprinus asiaticus Bleeker) were exposed to TDG supersaturated water at levels ranging from 120% to 145% for 48 h.The median lethal concentration(LC 50) and the median lethal time(LT 50) were determined to evaluate acute lethal effects on Chinese suckers.The results showed that the LC 50 values of 4,6,8,and 10 h were 142%,137%,135%,and 130%,respectively.The LT 50 values were 3.2,4.7,7.8,9.2,and 43.4 h,respectively,when TDG supersaturated levels were 145%,140%,135%,130%,and 125%.Furthermore,the biological responses in Chinese suckers were studied by assaying the catalase(CAT) activities in gills and muscles at the supersaturation level of 140% within LT 50.The CAT activities in the gills and muscle tissues exhibited a regularity of a decrease after an increase.CAT activities in the muscles were increased significantly at 3/5LT 50(P<0.05) and then came back to the normal level.However,there were no significant differences between the treatment group(TDG level of 140%) and the control group(TDG level of 100%) on CAT activities in the gills before 3/5LT 50(P>0.05),but the activities were significantly lower than the normal level at 4/5LT 50 and LT 50(P<0.05).展开更多
文摘为了明确绣球〔Hydrangea macrophylla(Thunb.)Ser.〕品种的耐寒性及筛选耐寒性评价主要指标,对40个绣球品种的低温半致死温度(LT_(50))进行比较和耐寒性分级,在此基础上,对5℃、0℃、-5℃、-10℃、-15℃、-20℃下耐寒品种‘小町’(‘Komachi’)、‘佳澄’(‘Kasumi’)、‘无尽夏’(‘Endless Summer’)和不耐寒品种‘甜蜜幻想’(‘Sweet Fantasy’)、‘灵感’(‘Inspiration’)、‘你我的情感’(‘You and Me Feelings’)叶的抗氧化酶活性及丙二醛(MDA)和渗透调节物质含量进行比较,并对这些耐寒指标进行相关性分析和主成分分析。结果表明:供试绣球品种LT 50的波动较大,变化范围为-36.68℃~-1.87℃,其中,‘小町’的LT_(50)最低,‘佳澄’、‘平瓣’(‘Flat’)、‘蒂亚娜’(‘Tijana’)、‘塞尔玛’(‘Selma’)、‘含羞叶’(‘Elbtal’)、‘花手鞠’(‘Stockings’)、‘蓝色多瑙河’(‘Blue Danube’)和‘无尽夏’的LT_(50)均低于-20℃。40个绣球品种的耐寒性被分成不耐寒、较耐寒、耐寒和强耐寒4个等级,分别包含13、18、8和1个品种。6个代表品种的过氧化氢酶(CAT)、多酚氧化酶(PPO)和超氧化物歧化酶活性及MDA、脯氨酸(Pro)、可溶性蛋白质(SP)和可溶性糖含量随温度降低基本呈先升高后降低的变化趋势。相关性分析结果表明PPO活性和Pro含量与LT_(50)呈极显著(P<0.01)负相关。主成分分析结果表明:前4个主成分的累计贡献率为81.212%,说明这4个主成分基本能够反映绣球品种的耐寒性;PPO活性、CAT活性、Pro含量和SP含量的负荷量绝对值分别在第1、第2、第3和第4主成分中最高。研究结果显示:供试绣球品种间的耐寒性差异较大,‘小町’等9个品种的耐寒性较强,能够在冬季温度偏低地区露天栽培,并可作为绣球耐寒品种选育的亲本。PPO活性、CAT活性、Pro含量和SP含量为绣球耐寒性评价的主要指标,尤其是PPO活性和Pro含量。
基金Project supported by the National Natural Science Foundation ofChina (No. 50979063)the Scientific Research Foundation for Young Teachers of Xihua University (No. Z1120412),China
文摘Total dissolved gas(TDG) supersaturation caused by dam sluicing can result in gas bubble trauma(GBT) in fish and threaten their survival.In the present study,Chinese suckers(Myxocyprinus asiaticus Bleeker) were exposed to TDG supersaturated water at levels ranging from 120% to 145% for 48 h.The median lethal concentration(LC 50) and the median lethal time(LT 50) were determined to evaluate acute lethal effects on Chinese suckers.The results showed that the LC 50 values of 4,6,8,and 10 h were 142%,137%,135%,and 130%,respectively.The LT 50 values were 3.2,4.7,7.8,9.2,and 43.4 h,respectively,when TDG supersaturated levels were 145%,140%,135%,130%,and 125%.Furthermore,the biological responses in Chinese suckers were studied by assaying the catalase(CAT) activities in gills and muscles at the supersaturation level of 140% within LT 50.The CAT activities in the gills and muscle tissues exhibited a regularity of a decrease after an increase.CAT activities in the muscles were increased significantly at 3/5LT 50(P<0.05) and then came back to the normal level.However,there were no significant differences between the treatment group(TDG level of 140%) and the control group(TDG level of 100%) on CAT activities in the gills before 3/5LT 50(P>0.05),but the activities were significantly lower than the normal level at 4/5LT 50 and LT 50(P<0.05).
