Thoughts on the Construction of Evidence-based Nursing Standard Plan for Dominant Diseases in Traditional Chinese Medicine

The evidence-based Traditional Chinese Medicine(TCM) nursing in standard plan is to be constructed by a combination of evidence-based thinking and expert consensus.(1) The symptoms of diseases with better curative effect in TCM and the nursing techniques of TCM based on scientific evidence can be preliminarily presented through bibliometric analysis.(2) Expert consultation letters can be developed based on bibliometric results, and symptoms of dominant diseases in TCM and TCM nursing techniques can be selected through those letters.(3) Based on the selected symptoms and TCM nursing techniques, the PICO can be determined, and then evidence search, evidence extraction, evidence analysis can be conducted and synthesized, and recommendations would be chosen.(4) The recommendation grade of each recommendation can be determined by applying the Delphi method and referring to GRADE.(5) The plan will be determined through discussions of experts. The construction of TCM nursing plan would make sure that TCM nursing is scientific, and internationalize TCM nursing.

中药远志对锰中毒小鼠微管相关蛋白表达的影响

目的 探讨远志对锰中毒小鼠学习记忆和微管相关蛋白表达的影响.方法 成年雌性昆明小鼠60只,随机分为正常对照组（生理盐水）、锰中毒组、锰中毒＋远志高剂量组（10.0 g/kg）、锰中毒＋远志中剂量组（5.0 g/kg）、锰中毒＋远志低剂量组（2.5 g/kg）,每组12只,采用腹腔注射氯化锰（MnCl215 mg/kg）染毒.Morris水迷宫实验检测小鼠学习记忆能力,免疫荧光染色检测小鼠侧脑室室管膜下区（SVZ）和海马齿状回颗粒下区（SGZ）内微管相关蛋白Doublecortin（DCX）的表达.结果 与锰中毒组比较,远志高、中、低剂量组逃避潜伏期明显缩短,差异有统计学意义（P＜0.05）远志高、中、低剂量组穿越平台次数比锰中毒组明显增多,差异有统计学意义（P＜0.05）.远志高、中、低剂量组SVZ和SGZ的DCX表达明显多于锰中毒组,差异有统计学意义（P＜0.05）.结论 远志干预可改善锰中毒小鼠的学习记忆能力,其机制可能与其促进脑内DCX的表达及神经发生有关.