A new microelement method for the analyses of functionally graded structures was proposed. The key of this method is the maneuverable combination of two kinds of elements. Firstly, the macro elements are divided from ...A new microelement method for the analyses of functionally graded structures was proposed. The key of this method is the maneuverable combination of two kinds of elements. Firstly, the macro elements are divided from the functionally graded material structures by the normal finite elements. In order to reflect the functionally graded distributions of materials and the microconstitutions in each macro-element, the microelement method sets up the dense microelements in every macro-element, and translates nodes to the same as the normal finite elements by the degrees of freedom of all microelemental the compatibility conditions. This microelement method can fully reflect the micro constitutions and different components of materials, and its computational elements are the same as the normal finite elements, so it is an effective numerical method for the analyses of the functionally graded material structures. The three-dimensional analyses of functionally graded plates with medium components and different micro net structures are given by using the microelement method in this paper. The differences of the stress contour in the plane of functionally graded plates with different net microstructures are especially given in this paper.展开更多
The optimization of nutrient levels for the production of recombinant hyperthermophilie esterase by E. coli was carried out with response surface methodology（RSM） based on the central composite rotatable design（CCR...The optimization of nutrient levels for the production of recombinant hyperthermophilie esterase by E. coli was carried out with response surface methodology（RSM） based on the central composite rotatable design（CCRD）. A 24 central composite rotatable design was used to study the combined effect of the nutritional constituents like yeast extract, peptone, mineral salt and trace metals. The P-value of the coefficient for the linear effect of peptone concentration was 0. 0081 and trace metals solution was less than 0. 0001, suggesting that these were the principal variables with significant effect on the hyperthermophilic esterase production. The predicted optimal hyperthermophilie esterase yield was 269. 17 U/mL, whereas an actual experimental value of 284. 58 U/mL was obtained.展开更多
基金supported by the National Natural Science Foundation of China (No.10432030)the National Youth Science Foundation of China (No.10802091)the Scientific and Technical Foundation of China University of Mining and Technology (No.2007B013)
文摘A new microelement method for the analyses of functionally graded structures was proposed. The key of this method is the maneuverable combination of two kinds of elements. Firstly, the macro elements are divided from the functionally graded material structures by the normal finite elements. In order to reflect the functionally graded distributions of materials and the microconstitutions in each macro-element, the microelement method sets up the dense microelements in every macro-element, and translates nodes to the same as the normal finite elements by the degrees of freedom of all microelemental the compatibility conditions. This microelement method can fully reflect the micro constitutions and different components of materials, and its computational elements are the same as the normal finite elements, so it is an effective numerical method for the analyses of the functionally graded material structures. The three-dimensional analyses of functionally graded plates with medium components and different micro net structures are given by using the microelement method in this paper. The differences of the stress contour in the plane of functionally graded plates with different net microstructures are especially given in this paper.
文摘The optimization of nutrient levels for the production of recombinant hyperthermophilie esterase by E. coli was carried out with response surface methodology（RSM） based on the central composite rotatable design（CCRD）. A 24 central composite rotatable design was used to study the combined effect of the nutritional constituents like yeast extract, peptone, mineral salt and trace metals. The P-value of the coefficient for the linear effect of peptone concentration was 0. 0081 and trace metals solution was less than 0. 0001, suggesting that these were the principal variables with significant effect on the hyperthermophilic esterase production. The predicted optimal hyperthermophilie esterase yield was 269. 17 U/mL, whereas an actual experimental value of 284. 58 U/mL was obtained.
