Studies on arsenic trioxide induced mice melanoma Cloundman S91 cell apoptosis

Objective: To investigate the effects of various concentrations As 2O 3 on malignant melanoma. Methods: The viability of Cloundman melanoma S91 cells treated with As 2O 3 was measured by MTT [3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide] assay. The apoptosis was determined by transferase-mediated dUTP nick end labeling (TUNEL) detection, and the morphology of apoptotic cells was observed through transmission electron microscopy (TEM). The cells growth phase were analyzed by flow cytometry (FCM). Results: A time-and dose-dependent decrease in cell viability was induced in S91 cells after treatment with As 2O 3 at the concentration of 1-5 μmol/L respectively. The TUNEL indices were 0.033±0.018, 0.062±0.012, 0.102±0.016, 0.132±0.031, and 0.162±0.027 respectively, which were much higher compared with the control group (0.017±0.004, P<0.01). The flow cytometry showed that hypodiploid peak after treatment with 3 μmol/L and 5 μmol/L of As 2O 3 for 48 h were 9.99% and 17.59% respectively, which increased significantly compared with the control cells (3.05% P<0.01,). The apoptotic morphology observed by transmission electron microscope showed the chromatin became condensed and attached to the inner surface of nuclear membrane. Conclusion: Arsenic trioxide can induce melanoma Cloundman S91 cell apoptosis at the concentration of 1-5 μmol/L, which will provide enhanced benefit in melanoma therapy.

顺铂诱导小鼠黑素瘤Cloundman S91细胞株凋亡的研究

目的:探讨顺铂CDDP对小鼠黑素瘤CloundmanS91细胞株的作用。方法:采用四甲基偶氮唑蓝MTT还原法检测CDDP对S91细胞株存活率的影响,吖啶橙染色观察凋亡细胞形态,流式细胞仪分析凋亡细胞周期,末端脱氧核苷转移酶TdT介导的脱氧尿苷三磷酸dUTP末端标记法TUNEL原位观察,计算细胞平均凋亡指数。结果:S91细胞经CDDP处理后,存活率降低,并与药物浓度呈正相关;荧光显微镜下可见明显的凋亡细胞;细胞周期G1期前出现凋亡峰。CDDP浓度在10、20、30μmol/L时,细胞平均凋亡指数分别为11.5%、19.8%、33.7%。结论:10～30μmol/L的CDDP诱导S91黑素瘤细胞凋亡。