Objective: To elucidate the molecular mechanism(s) by which methyl salicylate enhances the skin delivery of herbal ingredients with diverse lipophilicity.Methods: The toxicity of methyl salicylate on skin cell lines w...Objective: To elucidate the molecular mechanism(s) by which methyl salicylate enhances the skin delivery of herbal ingredients with diverse lipophilicity.Methods: The toxicity of methyl salicylate on skin cell lines was evaluated using the MTT assay. The Franz diffusion cell method was used to measure the permeability enhancing activities of methyl salicylate for five herbal ingredients with a range of lipophilicities. The interaction between methyl salicylate and the stratum corneum(SC) was observed by using an infrared spectroscopy technique. Moreover, the solubilities and SC-vehicle partition coefficient were determined to monitor the impact of methyl salicylate on the drug thermodynamic activities and partition into the SC layer, respectively.Results: Compared with azone(1-dodecylazacycloheptan-2-one), methyl salicylate showed lower toxicity to skin cells in terms of the IC50 values. The in vitro skin permeation studies showed that methyl salicylate could greatly improve the cumulative amounts or steady state flux of the selected model drugs with the exception of osthole, which indicated that methyl salicylate was prone to promote the skin delivery of hydrophilic drugs. The Fourier transform infrared spectroscopy studies revealed that methyl salicylate mainly interacted with SC lipids, leading to the disruption of the orderly arrangement of the SC.In addition, methyl salicylate had no obvious effect on the drug thermodynamic activity and partition into the SC.Conclusion: Methyl salicylate could effectively promote the skin delivery of relatively hydrophilic ingredients in externally used traditional Chinese medicines(TCM) without obvious cytotoxicity.展开更多
We evaluated the biomass and ergosterol content of Hericium erinaceus mycelium, and extracellular enzyme activities in H. erinaceus liquid culture following salicylic acid（SA） and methyl jasmonic acid（Me JA）supple...We evaluated the biomass and ergosterol content of Hericium erinaceus mycelium, and extracellular enzyme activities in H. erinaceus liquid culture following salicylic acid（SA） and methyl jasmonic acid（Me JA）supplementation. The optimal SA concentration was100 lmoláL-1, where the highest ergosterol content of 2.33 mgág-1was obtained following 6-day cultivation with100 lmoláL-1SA supplementation, and which was significantly higher than the unsupplemented control（p ／ 0.01）. Following 4-day supplementation with50 lmoláL-1Me JA, the highest ergosterol content obtained was 1.988 mgág-1, which was 25.8 % higher than the unsupplemented control. Our data indicate that SA and Me JA supplementation improves ergosterol content in H.erinaceus mycelium.展开更多
The contents of seven different phenolic acids such as gallic acid, catechinic acid, pyrocatechol, caffeic acid, coumaric acid, ferulic acid and benzoic acid in the poplar leaves （Populus Simonii×Populus Pyramib...The contents of seven different phenolic acids such as gallic acid, catechinic acid, pyrocatechol, caffeic acid, coumaric acid, ferulic acid and benzoic acid in the poplar leaves （Populus Simonii×Populus Pyramibalis c.v and Populus deltoids） suffocated by Methyl jasmonate （MeJA） and Methyl salicylate （MeSA） were monitored for analyzing their functions in interplant communications by using high-pressure liquid chromatography （HPLC）.The results showed that the contents of phenolic acids had obviously difference in leaves exposed to either MeSA or MeJA.When P.deltoides leaves exposed to MeJA or MeSA, the level of gallic acid, coumaric acid, caffeic acid, ferulic acid and benzoic acid was increased, gallic acid in leaves treated with MeJA comes to a peak at 24 h while to a peak at 12-d having leaves treated with MeSA.When P.Simonii ×P.Pyramibalis c.v leaves were exposed to MeJA or MeSA, the level of gallic acid, pyrocatechol and ferulic acid was increased; The catechinic acid and benzoic acid had a little drop; The caffeic acid and coumaric acid were undetected in both suffocated and control leaves.This changed pattern indicated that MeJA and MeSA can act as airborne signals to induce defense response of plants.展开更多
Efficient chemicalwarfare agents(CWAs)detection is required to protect people from the cWAs in war and terrorism.In this work,a Pd-doped SnO_(2)nanoparticles-based gas sensor was developed to detect a nerve agent simu...Efficient chemicalwarfare agents(CWAs)detection is required to protect people from the cWAs in war and terrorism.In this work,a Pd-doped SnO_(2)nanoparticles-based gas sensor was developed to detect a nerve agent simulant named methyl salicylate.The sensing measurements of methyl salicylate under different Pd doping amounts found that the 0.5 at.%Pd-doped SnO_(2)exhibited a significant improvement in the detection of methyl salicylate at the ppb(1ppb=10-9)level,and the response value to 160 ppb methyl salicylate is 0.72 at 250℃.Compared with the pure SnO_(2),the response value is increased by 4.5 times,which could be attributed to the influence of the noble metal Pd on the oxygen state and its catalytic effect.In addition,the 0.5at.%Pd-doped SnO_(2)sensor still has an obvious response to 16ppb methyl salicylate with a response value of 0.13,indicating the lower detection limit of the sensor.展开更多
基金supported by the National Natural Science Foundation of China(81473365)the Innovative Research Team in Beijing University of Chinese Medicine(2011-CXTD-13)。
文摘Objective: To elucidate the molecular mechanism(s) by which methyl salicylate enhances the skin delivery of herbal ingredients with diverse lipophilicity.Methods: The toxicity of methyl salicylate on skin cell lines was evaluated using the MTT assay. The Franz diffusion cell method was used to measure the permeability enhancing activities of methyl salicylate for five herbal ingredients with a range of lipophilicities. The interaction between methyl salicylate and the stratum corneum(SC) was observed by using an infrared spectroscopy technique. Moreover, the solubilities and SC-vehicle partition coefficient were determined to monitor the impact of methyl salicylate on the drug thermodynamic activities and partition into the SC layer, respectively.Results: Compared with azone(1-dodecylazacycloheptan-2-one), methyl salicylate showed lower toxicity to skin cells in terms of the IC50 values. The in vitro skin permeation studies showed that methyl salicylate could greatly improve the cumulative amounts or steady state flux of the selected model drugs with the exception of osthole, which indicated that methyl salicylate was prone to promote the skin delivery of hydrophilic drugs. The Fourier transform infrared spectroscopy studies revealed that methyl salicylate mainly interacted with SC lipids, leading to the disruption of the orderly arrangement of the SC.In addition, methyl salicylate had no obvious effect on the drug thermodynamic activity and partition into the SC.Conclusion: Methyl salicylate could effectively promote the skin delivery of relatively hydrophilic ingredients in externally used traditional Chinese medicines(TCM) without obvious cytotoxicity.
基金supported by funding from the China Agriculture Research System(CARS-24)the Heilongjiang Province Outstanding Youth Science Fund(JC201316)
文摘We evaluated the biomass and ergosterol content of Hericium erinaceus mycelium, and extracellular enzyme activities in H. erinaceus liquid culture following salicylic acid（SA） and methyl jasmonic acid（Me JA）supplementation. The optimal SA concentration was100 lmoláL-1, where the highest ergosterol content of 2.33 mgág-1was obtained following 6-day cultivation with100 lmoláL-1SA supplementation, and which was significantly higher than the unsupplemented control（p ／ 0.01）. Following 4-day supplementation with50 lmoláL-1Me JA, the highest ergosterol content obtained was 1.988 mgág-1, which was 25.8 % higher than the unsupplemented control. Our data indicate that SA and Me JA supplementation improves ergosterol content in H.erinaceus mycelium.
基金This research is supported by National Natural Science Foundation of China (No.30170764)
文摘The contents of seven different phenolic acids such as gallic acid, catechinic acid, pyrocatechol, caffeic acid, coumaric acid, ferulic acid and benzoic acid in the poplar leaves （Populus Simonii×Populus Pyramibalis c.v and Populus deltoids） suffocated by Methyl jasmonate （MeJA） and Methyl salicylate （MeSA） were monitored for analyzing their functions in interplant communications by using high-pressure liquid chromatography （HPLC）.The results showed that the contents of phenolic acids had obviously difference in leaves exposed to either MeSA or MeJA.When P.deltoides leaves exposed to MeJA or MeSA, the level of gallic acid, coumaric acid, caffeic acid, ferulic acid and benzoic acid was increased, gallic acid in leaves treated with MeJA comes to a peak at 24 h while to a peak at 12-d having leaves treated with MeSA.When P.Simonii ×P.Pyramibalis c.v leaves were exposed to MeJA or MeSA, the level of gallic acid, pyrocatechol and ferulic acid was increased; The catechinic acid and benzoic acid had a little drop; The caffeic acid and coumaric acid were undetected in both suffocated and control leaves.This changed pattern indicated that MeJA and MeSA can act as airborne signals to induce defense response of plants.
基金supported by the Science and Technology on Aerospace Chemical Power Laboratory,Hubei Institute of Aerospace Chemical Technology(U20B2018)the Open Research Fund Program of Science and Technology on Aerospace Chemical Power Laboratory(STACPL320201B02 and STACPL320181B03-1)the National Natural Science Foundation of China(Nos.61971204 and 51902114).
文摘Efficient chemicalwarfare agents(CWAs)detection is required to protect people from the cWAs in war and terrorism.In this work,a Pd-doped SnO_(2)nanoparticles-based gas sensor was developed to detect a nerve agent simulant named methyl salicylate.The sensing measurements of methyl salicylate under different Pd doping amounts found that the 0.5 at.%Pd-doped SnO_(2)exhibited a significant improvement in the detection of methyl salicylate at the ppb(1ppb=10-9)level,and the response value to 160 ppb methyl salicylate is 0.72 at 250℃.Compared with the pure SnO_(2),the response value is increased by 4.5 times,which could be attributed to the influence of the noble metal Pd on the oxygen state and its catalytic effect.In addition,the 0.5at.%Pd-doped SnO_(2)sensor still has an obvious response to 16ppb methyl salicylate with a response value of 0.13,indicating the lower detection limit of the sensor.
