[Objective]This study aimed to investigate the major factors influencing the proliferation and growth of aseptic buds of Vietnam Mesona blumes,thus providing technical references for establishing rapid propagation sys...[Objective]This study aimed to investigate the major factors influencing the proliferation and growth of aseptic buds of Vietnam Mesona blumes,thus providing technical references for establishing rapid propagation system in vitro of Vietnam M.blumes.[Method]The effects of medium components（different basic medium,sucrose concentrations and cytokinins）and explant materials（different explants）on the proliferation of aseptic buds of Vietnam M.blumes were analyzed.[Result]Among MS,Miller and modified White basic medium,MS medium exhibited the best effect on proliferation of Vietnam M.blumes buds with the bud proliferation multiple of 8.53.Among different sucrose concentrations,20.0 g/L sucrose gave the highest bud proliferation multiple of 8.34,while 25.0 g/L sucrose led to the best growth status with the bud proliferation multiple of 7.05.The effect of CPPU on bud proliferation was higher than that of 6-BA and KT,with the bud proliferation multiple of 9.80.Among different explants,top stem exhibited the best effect on bud proliferation with the bud proliferation multiple of 9.35,resulting in robust seedlings.[Conclusion]The most suitable subculture medium for the proliferation and growth of aseptic buds of Vietnam M.blumes is MS＋20.0-25.0 g/L sucrose＋0.5 mg/L CPPU and the most suitable explant is top stem.展开更多
在有盐环境下贮藏和加工凉粉草胶必须了解其多糖分子对盐的敏感性,该文绘制了凉粉草胶多糖在不同离子强度N aC l、KC l、M gC l2和C aC l2溶液中的Hugg ins'图,分别计算其分子链在这4种离子溶液中的耐盐度S、链刚性度B等值,结果发现...在有盐环境下贮藏和加工凉粉草胶必须了解其多糖分子对盐的敏感性,该文绘制了凉粉草胶多糖在不同离子强度N aC l、KC l、M gC l2和C aC l2溶液中的Hugg ins'图,分别计算其分子链在这4种离子溶液中的耐盐度S、链刚性度B等值,结果发现:凉粉草胶多糖在4种离子溶液中,其耐盐性由强到弱的顺序为:K+>N a+>M g++>C a++,其分子链的构象在4种离子溶液中由柔到刚的顺序为:K+>N a+>M g++>C a++,这说明凉粉草胶多糖适合在KC l溶液中贮藏和加工。与其他多糖分子链在N aC l溶液中的B值比较后发现,凉粉草胶多糖的分子链构象较海藻酸钠要刚,较黄原胶要柔,这说明凉粉草胶比黄原胶更适合在N aC l溶液中贮藏和加工。展开更多
基金Supported by Scientific Research and Technological Development Project of Wuzhou City,Guangxi Zhuang Autonomous Region(200901024)
文摘[Objective]This study aimed to investigate the major factors influencing the proliferation and growth of aseptic buds of Vietnam Mesona blumes,thus providing technical references for establishing rapid propagation system in vitro of Vietnam M.blumes.[Method]The effects of medium components（different basic medium,sucrose concentrations and cytokinins）and explant materials（different explants）on the proliferation of aseptic buds of Vietnam M.blumes were analyzed.[Result]Among MS,Miller and modified White basic medium,MS medium exhibited the best effect on proliferation of Vietnam M.blumes buds with the bud proliferation multiple of 8.53.Among different sucrose concentrations,20.0 g/L sucrose gave the highest bud proliferation multiple of 8.34,while 25.0 g/L sucrose led to the best growth status with the bud proliferation multiple of 7.05.The effect of CPPU on bud proliferation was higher than that of 6-BA and KT,with the bud proliferation multiple of 9.80.Among different explants,top stem exhibited the best effect on bud proliferation with the bud proliferation multiple of 9.35,resulting in robust seedlings.[Conclusion]The most suitable subculture medium for the proliferation and growth of aseptic buds of Vietnam M.blumes is MS＋20.0-25.0 g/L sucrose＋0.5 mg/L CPPU and the most suitable explant is top stem.
文摘在有盐环境下贮藏和加工凉粉草胶必须了解其多糖分子对盐的敏感性,该文绘制了凉粉草胶多糖在不同离子强度N aC l、KC l、M gC l2和C aC l2溶液中的Hugg ins'图,分别计算其分子链在这4种离子溶液中的耐盐度S、链刚性度B等值,结果发现:凉粉草胶多糖在4种离子溶液中,其耐盐性由强到弱的顺序为:K+>N a+>M g++>C a++,其分子链的构象在4种离子溶液中由柔到刚的顺序为:K+>N a+>M g++>C a++,这说明凉粉草胶多糖适合在KC l溶液中贮藏和加工。与其他多糖分子链在N aC l溶液中的B值比较后发现,凉粉草胶多糖的分子链构象较海藻酸钠要刚,较黄原胶要柔,这说明凉粉草胶比黄原胶更适合在N aC l溶液中贮藏和加工。
