A total of 110 elderly people with hyperlipidemia were randomly assigned to one of two groups. The experimental group consumed an ordinary diet plus foods containing refined Konjac meal, and the control group consumed...A total of 110 elderly people with hyperlipidemia were randomly assigned to one of two groups. The experimental group consumed an ordinary diet plus foods containing refined Konjac meal, and the control group consumed only the ordinary diet. The experiment was carried out for 45 days. The results indicate that for the experimental group blood levels of triglyeendes (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) were significantly lowered (P < 0.01) at the end of the trial, whereas high-density lipoprotein cholesterol (HDL-C) and apoprotein (Al) levels were significantly elevated (P<0.01). In contrast, for the control group, the changes in the above parameters were insignificant. The differences in TC. TG, LDL-C, and HDL-C levels between the two groups were statistically significant. The effects of refined Konjac meal on lipid levels in the blood were somewhat different between patients with hyperlipidemia and subjects with risk critical values only. For the former, TG and TC were decreased by 83.8±133.5 mg/dl, and 42.4±23.4 mg/dl. respectively: but for the latter, they are decreased only by -1.1±23.1 mg/dl and 8.3± 18.2 mg/dl, respectively; the difference mentioned above is Statistically significant (P<0.01). 1990 Academic Press. Inc.展开更多
To help researchers in the field of biology,medicine,chemistry,and materials science to use lipidomic data conveniently,there is an urgent need to develop a platform that provides a systematic knowledgebase of human l...To help researchers in the field of biology,medicine,chemistry,and materials science to use lipidomic data conveniently,there is an urgent need to develop a platform that provides a systematic knowledgebase of human lipid metabolism and lipidome-centric omics analysis tools.DBLiPro is a user-friendly webserver allowing for access to human metabolism-related lipids and proteins knowledge database and an interactive bioinformatics integrative analysis workflow for lipidomics,transcriptomics,and proteomics data.In DBLiPro,there are 3109 lipid-associated proteins(LAPs)and 2098 lipid metabolites in the knowledge base section,which were obtained from Uniprot,Kyoto Encyclopedia of Genes and Genomes(KEGG)and were further annotated by information from other public resources in the knowledge base section,such as RaftProt and PubChem.DBLiPro offers a step-by-step interactive analysis workflow for lipidomics,transcriptomics,proteomics,and their integrating multi-omics analysis focusing on the human lipid metabolism.In summary,DBLiPro is capable of helping users discover key molecules(lipids and proteins)in human lipid metabolism and investigate lipid–protein functions underlying mechanisms based on their own omics data.The DBLiPro is freely available at http://lipid.cloud na.cn/home.展开更多
Background: Impaired insulin sensitivity may partly arise from a dysregulated lipid metabolism in human skeletal muscle. This study investigates the expression levels of perilipin 2, 3, and 5, and four key lipases in ...Background: Impaired insulin sensitivity may partly arise from a dysregulated lipid metabolism in human skeletal muscle. This study investigates the expression levels of perilipin 2, 3, and 5, and four key lipases in human skeletal muscle from the subjects that exhibit a range from normal to very low insulin sensitivity. Methods: 25 middle aged male participants were matched for lean body mass and recruited into three groups;type 2 diabetes patients (T2D), impaired glucose tolerance (IGT), and healthy sedentary controls (CON) according to their glucose tolerance and VO2peak. A muscle biopsy was obtained from vastus lateralis, and a two-step sequential euglycaemic-hyperinsulinaemic clamp was performed. Muscle samples were analyzed by Western blot for expression of perilipin 2, 3, 5, adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), endothelial lipase (EL) and lipoprotein lipase (LPL). Results: Perilipin 3 expression was higher in T2D compared to CON. Perilipin 2 expression was higher in CON than T2D. We observed no difference in expression of perili pin 5, ATGL, HSL, EL or LPL between the groups. Conclusions: In the present study the muscle perilipin 3 expression and perilipin 2 expression varied markedly with insulin sensitivity. This difference in perilipin expression may indicate that the lipid droplet function and thus storage and release of fatty acid-vary with insulin sensitivity.展开更多
Ferroptosis is defined as an iron-dependent regulated form of cell death driven by lipid peroxidation.In the past decade,it has been implicated in the pathogenesis of various diseases that together involve almost ever...Ferroptosis is defined as an iron-dependent regulated form of cell death driven by lipid peroxidation.In the past decade,it has been implicated in the pathogenesis of various diseases that together involve almost every organ of the body,including various cancers,neurodegenerative diseases,cardiovascular diseases,lung diseases,liver diseases,kidney diseases,endocrine metabolic diseases,iron-overload-related diseases,orthopedic diseases and autoimmune diseases.Understanding the underlying molecular mechanisms of ferroptosis and its regulatory pathways could provide additional strategies for the management of these disease conditions.Indeed,there are an expanding number of studies suggesting that ferroptosis serves as a bona-fide target for the prevention and treatment of these diseases in relevant pre-clinical models.In this review,we summarize the progress in the research into ferroptosis and its regulatory mechanisms in human disease,while providing evidence in support of ferroptosis as a target for the treatment of these diseases.We also discuss our perspectives on the future directions in the targeting of ferroptosis in human disease.展开更多
文摘A total of 110 elderly people with hyperlipidemia were randomly assigned to one of two groups. The experimental group consumed an ordinary diet plus foods containing refined Konjac meal, and the control group consumed only the ordinary diet. The experiment was carried out for 45 days. The results indicate that for the experimental group blood levels of triglyeendes (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) were significantly lowered (P < 0.01) at the end of the trial, whereas high-density lipoprotein cholesterol (HDL-C) and apoprotein (Al) levels were significantly elevated (P<0.01). In contrast, for the control group, the changes in the above parameters were insignificant. The differences in TC. TG, LDL-C, and HDL-C levels between the two groups were statistically significant. The effects of refined Konjac meal on lipid levels in the blood were somewhat different between patients with hyperlipidemia and subjects with risk critical values only. For the former, TG and TC were decreased by 83.8±133.5 mg/dl, and 42.4±23.4 mg/dl. respectively: but for the latter, they are decreased only by -1.1±23.1 mg/dl and 8.3± 18.2 mg/dl, respectively; the difference mentioned above is Statistically significant (P<0.01). 1990 Academic Press. Inc.
基金supported by the National Natural Science Foundation of China(No.32000472 to WD and No.32100531 to YC)Innovation Promotion Program of NHC and Shanghai Key Labs SIBPT(RC2023-01 to WD)+1 种基金Shanghai Academy of Science&Technology(SKY2022003 to WD)Innovation-oriented Science and Technology Grant from NHC Key Lab of Reproduction Regulation(CX2022-02 to YYL).
文摘To help researchers in the field of biology,medicine,chemistry,and materials science to use lipidomic data conveniently,there is an urgent need to develop a platform that provides a systematic knowledgebase of human lipid metabolism and lipidome-centric omics analysis tools.DBLiPro is a user-friendly webserver allowing for access to human metabolism-related lipids and proteins knowledge database and an interactive bioinformatics integrative analysis workflow for lipidomics,transcriptomics,and proteomics data.In DBLiPro,there are 3109 lipid-associated proteins(LAPs)and 2098 lipid metabolites in the knowledge base section,which were obtained from Uniprot,Kyoto Encyclopedia of Genes and Genomes(KEGG)and were further annotated by information from other public resources in the knowledge base section,such as RaftProt and PubChem.DBLiPro offers a step-by-step interactive analysis workflow for lipidomics,transcriptomics,proteomics,and their integrating multi-omics analysis focusing on the human lipid metabolism.In summary,DBLiPro is capable of helping users discover key molecules(lipids and proteins)in human lipid metabolism and investigate lipid–protein functions underlying mechanisms based on their own omics data.The DBLiPro is freely available at http://lipid.cloud na.cn/home.
基金Financial support from The 1991 Pharmacy Foundation,NOVO Nordic Foundation,Direktor Verner Richter and Hustrus foundation,the foundation of 1870,Direktor Jacob and Olga Madsens foundation,Aase and Ejnar Danielsens foundation and EU project EXGENESIS 005272 is gratefully acknowledged
文摘Background: Impaired insulin sensitivity may partly arise from a dysregulated lipid metabolism in human skeletal muscle. This study investigates the expression levels of perilipin 2, 3, and 5, and four key lipases in human skeletal muscle from the subjects that exhibit a range from normal to very low insulin sensitivity. Methods: 25 middle aged male participants were matched for lean body mass and recruited into three groups;type 2 diabetes patients (T2D), impaired glucose tolerance (IGT), and healthy sedentary controls (CON) according to their glucose tolerance and VO2peak. A muscle biopsy was obtained from vastus lateralis, and a two-step sequential euglycaemic-hyperinsulinaemic clamp was performed. Muscle samples were analyzed by Western blot for expression of perilipin 2, 3, 5, adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), endothelial lipase (EL) and lipoprotein lipase (LPL). Results: Perilipin 3 expression was higher in T2D compared to CON. Perilipin 2 expression was higher in CON than T2D. We observed no difference in expression of perili pin 5, ATGL, HSL, EL or LPL between the groups. Conclusions: In the present study the muscle perilipin 3 expression and perilipin 2 expression varied markedly with insulin sensitivity. This difference in perilipin expression may indicate that the lipid droplet function and thus storage and release of fatty acid-vary with insulin sensitivity.
基金support from the National Natural Science Foundation of China(No.31930057 to Fudi Wang and No.31970689 to Junxia Min)the National Key R&D Program(No.2018YFA0507801 to Junxia Min and No.2018YFA0507802 to Fudi Wang)the China Postdoctoral Science Foundation(No.2022M712733 to Xue Wang).
文摘Ferroptosis is defined as an iron-dependent regulated form of cell death driven by lipid peroxidation.In the past decade,it has been implicated in the pathogenesis of various diseases that together involve almost every organ of the body,including various cancers,neurodegenerative diseases,cardiovascular diseases,lung diseases,liver diseases,kidney diseases,endocrine metabolic diseases,iron-overload-related diseases,orthopedic diseases and autoimmune diseases.Understanding the underlying molecular mechanisms of ferroptosis and its regulatory pathways could provide additional strategies for the management of these disease conditions.Indeed,there are an expanding number of studies suggesting that ferroptosis serves as a bona-fide target for the prevention and treatment of these diseases in relevant pre-clinical models.In this review,we summarize the progress in the research into ferroptosis and its regulatory mechanisms in human disease,while providing evidence in support of ferroptosis as a target for the treatment of these diseases.We also discuss our perspectives on the future directions in the targeting of ferroptosis in human disease.
