We have developed a novel metal tag, bis(ethylenediamine)-4'-methyl-4-carboxybipyridine-ruthenium N-succinimidyl ester (ECRS) for sensitive analysis of amino acids using high performance liquid chromatography/indu...We have developed a novel metal tag, bis(ethylenediamine)-4'-methyl-4-carboxybipyridine-ruthenium N-succinimidyl ester (ECRS) for sensitive analysis of amino acids using high performance liquid chromatography/inductively coupled plasma mass spectrometry (HPLC/ICP-MS). ECRS is a functional reagent, containing an ester group at one end that can be activated to bind to amino group and a chelated ruthenium at the other. The activated ester was reacted briefly with amino groups under weakly alkaline conditions. The ruthenium was detected sensitively by ICP-MS. ECRS was reacted with 17 proteinogenic amino acids in borate buffer. The derivatives were separated by reversed phase HPLC and identified by quadrupole-based ICP-MS. ECRS was suitable for speciation;low molecular weight compounds containing amino groups. We have thus established a quantitative analytical method for amino acids and dipeptides. The detection limits of branched amino acids (signal-to-noise ratio of 3) were 1.5 nmol·L-1 in the standard solution (100 amol per injection).展开更多
Hitachi Information Systems started up the RFID business from 2 002 and provides varieties solutions, especially devoted the energies on the research of metallic tag and the development of its applied area.The metalli...Hitachi Information Systems started up the RFID business from 2 002 and provides varieties solutions, especially devoted the energies on the research of metallic tag and the development of its applied area.The metallic tag was developed to withstand the harsh environment like vibration,shock,high-pressure water injection,high voltage,and ultraviolet irradiation.Because the metallic tag that different with the metal tag created before,is protected by the metallic material that made it very strong to be used in the environment listed above. June,2006,our company successfully developed the metallic tag with armor-clad stainless steel.Therefore, comparing with the resin tag,the metallic tag with remarkable mechanical strength was sufficient attention to withstand the outdoor use,even to resist UV.By the method of screw fixing,rivet tightening,soldering or nail striking,the installation procedure can be completed.Because the various installation methods were supported,the metallic tag can be applied to many industry areas.The management markets like construction machinery, substation equipment,molds,tools,and metallic container that need the very strong tag can be developed,because of the metallic tag. In this thesis,it will be described about the research background and the research results of metallic tag,the commercialized metallic tag's features and application fields,as well as the research subjects in the future and recent development situation.展开更多
在通常的模具生产车间,模具电极只能依靠人来监控和管理,导致工作量大且效率低.为了解决这一问题,研究了无线射频识别技术在模具电极管理中的应用.利用微软Visual Studio 2008作为开发工具,采用B/S(浏览器/服务器)的系统架构,开发了一...在通常的模具生产车间,模具电极只能依靠人来监控和管理,导致工作量大且效率低.为了解决这一问题,研究了无线射频识别技术在模具电极管理中的应用.利用微软Visual Studio 2008作为开发工具,采用B/S(浏览器/服务器)的系统架构,开发了一套基于无线射频识别技术的模具电极管理系统.首先,对系统总体结构进行设计,包括主要硬件的选型及参数的设置;接着,对系统业务流程进行了规范,再根据业务流程进行了功能需求分析;然后,对系统各功能模块进行了划分,对数据库结构进行了设计;最后,运用NET技术开发了系统各界面及功能.实践证明该系统对电极的管理方便有效,提高了模具电极管理的智能化水平.展开更多
Immobilization metal affinity chromatography(IMAC)and size-exclusive chromatography(SEC)have been widely used in the purification of recombinant protein.In order to apply the column chromatography to the separation an...Immobilization metal affinity chromatography(IMAC)and size-exclusive chromatography(SEC)have been widely used in the purification of recombinant protein.In order to apply the column chromatography to the separation and purification of the gene recombinant with histidine-tags,the column chromatographic separation characteristics of N-terminal histidine-tagged(N-AxCeSD)and C-terminal histidine-tagged(C-AxCeSD)gene recombinant protein AxCeSD,one of the subunit involved in the cellulose synthesis in Acetobacter xylinum were studied.In the ring-shaped three-dimensional structure of AxCeSD,N-terminal histidine-tags were located in the inner of ring,while C-terminal histidine-tags were located in the outer.A higher imidazole concentration was necessary for eluting the C-AxCeSD from the IMAC column due to the C-terminal histidine-tags had stronger chelating interaction with the Ni2+ on the IMAC media.Moreover,the retention time for eluting C-AxCeSD from the same SEC gel column was shorter than that for N-AxCeSD,because the larger protein homolog was formed in the C-AxCeSD solution through the inter-molecular hydrogen bonds between the C-terminal histidine-tags.展开更多
在大肠杆菌中表达的重组Hepcidin融合蛋白以包涵体形式存在,其N端带有6个组氨酸。以Ni2+-IDA-Sepharose Fast Flow为层析介质,在变性条件下以不同的咪唑和pH值洗脱方式对Hepcidin融合蛋白的纯化效果进行了比较,确定了该融合蛋白的金属...在大肠杆菌中表达的重组Hepcidin融合蛋白以包涵体形式存在,其N端带有6个组氨酸。以Ni2+-IDA-Sepharose Fast Flow为层析介质,在变性条件下以不同的咪唑和pH值洗脱方式对Hepcidin融合蛋白的纯化效果进行了比较,确定了该融合蛋白的金属螯合层析纯化条件。以60 mmol/L咪唑洗脱杂蛋白,然后将pH值降至4.0洗脱融合蛋白,纯化后的融合蛋白纯度大于95%,而且不含咪唑,有利于下一步Hepcidin的制备。金属螯合层析中融合蛋白收率不低于90%。Ni2+-IDA-Sepharose Fast Flow对该融合蛋白的吸附量为30.4 mg/mL。展开更多
文摘We have developed a novel metal tag, bis(ethylenediamine)-4'-methyl-4-carboxybipyridine-ruthenium N-succinimidyl ester (ECRS) for sensitive analysis of amino acids using high performance liquid chromatography/inductively coupled plasma mass spectrometry (HPLC/ICP-MS). ECRS is a functional reagent, containing an ester group at one end that can be activated to bind to amino group and a chelated ruthenium at the other. The activated ester was reacted briefly with amino groups under weakly alkaline conditions. The ruthenium was detected sensitively by ICP-MS. ECRS was reacted with 17 proteinogenic amino acids in borate buffer. The derivatives were separated by reversed phase HPLC and identified by quadrupole-based ICP-MS. ECRS was suitable for speciation;low molecular weight compounds containing amino groups. We have thus established a quantitative analytical method for amino acids and dipeptides. The detection limits of branched amino acids (signal-to-noise ratio of 3) were 1.5 nmol·L-1 in the standard solution (100 amol per injection).
文摘Hitachi Information Systems started up the RFID business from 2 002 and provides varieties solutions, especially devoted the energies on the research of metallic tag and the development of its applied area.The metallic tag was developed to withstand the harsh environment like vibration,shock,high-pressure water injection,high voltage,and ultraviolet irradiation.Because the metallic tag that different with the metal tag created before,is protected by the metallic material that made it very strong to be used in the environment listed above. June,2006,our company successfully developed the metallic tag with armor-clad stainless steel.Therefore, comparing with the resin tag,the metallic tag with remarkable mechanical strength was sufficient attention to withstand the outdoor use,even to resist UV.By the method of screw fixing,rivet tightening,soldering or nail striking,the installation procedure can be completed.Because the various installation methods were supported,the metallic tag can be applied to many industry areas.The management markets like construction machinery, substation equipment,molds,tools,and metallic container that need the very strong tag can be developed,because of the metallic tag. In this thesis,it will be described about the research background and the research results of metallic tag,the commercialized metallic tag's features and application fields,as well as the research subjects in the future and recent development situation.
文摘在通常的模具生产车间,模具电极只能依靠人来监控和管理,导致工作量大且效率低.为了解决这一问题,研究了无线射频识别技术在模具电极管理中的应用.利用微软Visual Studio 2008作为开发工具,采用B/S(浏览器/服务器)的系统架构,开发了一套基于无线射频识别技术的模具电极管理系统.首先,对系统总体结构进行设计,包括主要硬件的选型及参数的设置;接着,对系统业务流程进行了规范,再根据业务流程进行了功能需求分析;然后,对系统各功能模块进行了划分,对数据库结构进行了设计;最后,运用NET技术开发了系统各界面及功能.实践证明该系统对电极的管理方便有效,提高了模具电极管理的智能化水平.
文摘设计了一款超薄的高增益UHF抗金属标签天线。标签天线以高介电常数的陶瓷为介质基板,由上层带有双T型槽缝的金属贴片、介质基板和下层金属贴片组成,天线平面尺寸为80 mm×40 mm,厚度仅为0.8 mm。天线结构简单,不需要短路通孔、短路销钉或短路贴片将天线上层贴片与地板相连,通过改变天线上层贴片T型槽缝的尺寸,可以对标签天线阻抗进行有效调节,并实现天线的低剖面。天线未加金属地板最大增益为3.4 d Bi,加载金属地板最大增益为6.9 d Bi,且加载金属地板前后天线的中心频率和阻抗带宽无明显变化。实际测试结果与仿真结果比较吻合,实测未加载金属地板状态下最大识别距离为4.5 m,加载金属地板状态下实测最大识别距离为6.8 m。
文摘Immobilization metal affinity chromatography(IMAC)and size-exclusive chromatography(SEC)have been widely used in the purification of recombinant protein.In order to apply the column chromatography to the separation and purification of the gene recombinant with histidine-tags,the column chromatographic separation characteristics of N-terminal histidine-tagged(N-AxCeSD)and C-terminal histidine-tagged(C-AxCeSD)gene recombinant protein AxCeSD,one of the subunit involved in the cellulose synthesis in Acetobacter xylinum were studied.In the ring-shaped three-dimensional structure of AxCeSD,N-terminal histidine-tags were located in the inner of ring,while C-terminal histidine-tags were located in the outer.A higher imidazole concentration was necessary for eluting the C-AxCeSD from the IMAC column due to the C-terminal histidine-tags had stronger chelating interaction with the Ni2+ on the IMAC media.Moreover,the retention time for eluting C-AxCeSD from the same SEC gel column was shorter than that for N-AxCeSD,because the larger protein homolog was formed in the C-AxCeSD solution through the inter-molecular hydrogen bonds between the C-terminal histidine-tags.
文摘在大肠杆菌中表达的重组Hepcidin融合蛋白以包涵体形式存在,其N端带有6个组氨酸。以Ni2+-IDA-Sepharose Fast Flow为层析介质,在变性条件下以不同的咪唑和pH值洗脱方式对Hepcidin融合蛋白的纯化效果进行了比较,确定了该融合蛋白的金属螯合层析纯化条件。以60 mmol/L咪唑洗脱杂蛋白,然后将pH值降至4.0洗脱融合蛋白,纯化后的融合蛋白纯度大于95%,而且不含咪唑,有利于下一步Hepcidin的制备。金属螯合层析中融合蛋白收率不低于90%。Ni2+-IDA-Sepharose Fast Flow对该融合蛋白的吸附量为30.4 mg/mL。
