Four imidazole cyclophanes have been synthesized by the oxidation of bis-imidazoline with barium manganate followed by cyclization with 2,6-bis(bromomethyl) pyridine in the presence of NaH. All the compounds were iden...Four imidazole cyclophanes have been synthesized by the oxidation of bis-imidazoline with barium manganate followed by cyclization with 2,6-bis(bromomethyl) pyridine in the presence of NaH. All the compounds were identified by IR, (HNMR)-H-1, MS and elemental analysis.展开更多
In the construction of biosensors, enzymes function as mediators converting biological signals generated by specific biological processes, into electrochemical signals. The ideology of bio-sensor design is retention o...In the construction of biosensors, enzymes function as mediators converting biological signals generated by specific biological processes, into electrochemical signals. The ideology of bio-sensor design is retention of electron transfer activity of the enzyme utilizing superior interfacial architecture. In this work a Schiff-base macromolecule has been synthesized by reflux of 2, 3-diaminonaphthalene and pyrrole-2-carboxaldehyde starting materials. The Schiff-base ligand was subsequently complexed with FeCl2?4H2O under reflux, to produce the Fe-Schiff-base complex. The Schiff-base ligand and Fe-Schiff-base complex were characterized using nuclear magnetic resonance (NMR) spectroscopy, Ultra Violet/Visible (UV/Vis) spectroscopy, Fourier transfer infrared resonance (FTIR) and electron energy loss spectroscopy (EELS) to confirm the structure of the synthesis products. NMR spectroscopy confirmed the imide linkage of Schiff-base formation as two symmetrical peaks at 8.1 and 7.7 ppm respectively. Comparison of starting materials and product spectra by UV/Vis spectroscopy confirmed the disappearance of the diaminonaphthalene peak at 250 nm as evidence of complete conversion to product. FTIR spectroscopy of the Schiff-base ligand confirmed the formation of the imine bond at 1595 cm-1. EELS spectra comparing FeCl2?4H2O and the Fe-Schiff-base complex, showed good agreement in the energy loss profiles associated with changes to the electronic arrangement of Fe d-orbitals. EDS clearly identified a spectral band for Fe (7 - 8 eV) in the Fe-Schiff-base complex. Electrochemical evaluation of the Fe-Schiff-base complex was compared to the electrochemical signature of denatured cytochrome-C using cyclic voltammetry and square wave voltammetry. The Fe2+/Fe3+ quasi-reversible behavior for iron in the metallated complex was observed at -0.430 V vs. Ag/AgCl, which is consistent with reference values for iron in macromolecular structures.展开更多
A method using synchrotron radiation X-ray fluorescence analysis to in situ determine trace elements during protein electrophoretically separating process was established. The distribution of elements in protein bands...A method using synchrotron radiation X-ray fluorescence analysis to in situ determine trace elements during protein electrophoretically separating process was established. The distribution of elements in protein bands for human liver cytosolic sample separated by SDS-PAGE was analyzed a-long polyacrylamide gel. The results showed that the protein fraction of peak III in cvtosol was mainly composed of metal ion Zn-associated proteins, being in agreement with that given by atomic absorption spectrometry. Thus, it demonstrated the feasibility of this novel technique for in situ analysis of trace elements in protein bands.展开更多
Cells contain a large number of metalloproteins that commonly harbor at least one metal ion cofactor. In metalloproteins, metal ions are usually coordinated by oxygen, sulfur, or nitrogen centers belonging to amino ac...Cells contain a large number of metalloproteins that commonly harbor at least one metal ion cofactor. In metalloproteins, metal ions are usually coordinated by oxygen, sulfur, or nitrogen centers belonging to amino acid residues in the protein. The presence of the metal ion in metalloproteins allows them to take part in diverse biological processes, such as genome stability, metabolic catalysis, and cell cycle progression. Clinically, alteration of the function of metalloproteins in mammals is genetically associated with diseases characterized by DNA damage and repair defects. The present review focuses on the current perspectives of metal ion homeostasis in different organisms and summarizes the most recent understanding on magnesium, copper, iron, and manganese-containing proteins and their functional involvement in the maintenance of genome stability.展开更多
In this study, we exhibited an amino acid (arginine and threonine) derivative Schiff base copper(II) complexes incorporating an azobenzene moiety as a photoresponsive site and conjugated it to egg white lysozyme, a we...In this study, we exhibited an amino acid (arginine and threonine) derivative Schiff base copper(II) complexes incorporating an azobenzene moiety as a photoresponsive site and conjugated it to egg white lysozyme, a well-known protein, to change ligand conformation under binding to lysozyme. Among several spectroscopic investigations, ESR clearly showed that the nitrogen atom of the amino acid residue of lysozyme was bound to the paramagnetic copper(II) ion of the complex, and UV light irradiation confirmed photoisomerization of the azobenzene moiety of the ligand to cis-form. The binding mode was considered by means of spectroscopic as well as computational methods, whereas complete crystallographic verification was still a preliminary stage.展开更多
Objective: To study the effects of operation combined with chemotherapy on MMP-2, TIMP-1, VEGF, NSE, LDH and immunologic function in children with neuroblastoma (NB). Methods: A total of 90 children with NB in our hos...Objective: To study the effects of operation combined with chemotherapy on MMP-2, TIMP-1, VEGF, NSE, LDH and immunologic function in children with neuroblastoma (NB). Methods: A total of 90 children with NB in our hospital from January 2014 to January 2017 were enrolled in this study. The subjects were divided into the control group (n=45) and the treatment group (n=45) randomly. The control group was treated with operation for 7 periods;the treatment group was treated with operation combined with chemotherapy for 7 periods. The serum MMP-2, TIMP-1, VEGF, NSE, LDH, IgG, IgA, IgM, IgE and peripheral blood TTL, THL, TSL, NK, CD4+/CD8+ of the two groups before and after treatment were compared. Results: There were no significantly differences of the serum MMP-2, TIMP-1, VEGF, NSE, LDH of the two groups before treatment. The serum MMP-2, TIMP-1, VEGF, NSE, LDH of the two groups after treatment were significantly lower than before treatment, and that of the treatment group after treatment were significantly lower than the control group. There were no significantly differences of the serum IgG, IgA, IgM, IgE of the two groups before treatment. The serum IgG, IgA, IgM, IgE of the two groups after treatment were significantly lower than before treatment, and that of the treatment group after treatment were significantly lower than the control group. There were no significantly differences of the peripheral blood TTL, THL, TSL, NK, CD4+/CD8+ of the two groups before treatment. The peripheral blood TTL, CD4+/CD8+ of the two groups after treatment were significantly lower than before treatment, the peripheral blood THL, TSL, NK of the two groups after treatment were significantly higher than before treatment, and that of the treatment group after treatment were significantly better than the control group. Conclusion: Operation combined with chemotherapy can inhabit the tumor infiltration and metastasis of the children with NB, can improve the cellular immunity function, and it was worthy clinical application.展开更多
文摘Four imidazole cyclophanes have been synthesized by the oxidation of bis-imidazoline with barium manganate followed by cyclization with 2,6-bis(bromomethyl) pyridine in the presence of NaH. All the compounds were identified by IR, (HNMR)-H-1, MS and elemental analysis.
文摘In the construction of biosensors, enzymes function as mediators converting biological signals generated by specific biological processes, into electrochemical signals. The ideology of bio-sensor design is retention of electron transfer activity of the enzyme utilizing superior interfacial architecture. In this work a Schiff-base macromolecule has been synthesized by reflux of 2, 3-diaminonaphthalene and pyrrole-2-carboxaldehyde starting materials. The Schiff-base ligand was subsequently complexed with FeCl2?4H2O under reflux, to produce the Fe-Schiff-base complex. The Schiff-base ligand and Fe-Schiff-base complex were characterized using nuclear magnetic resonance (NMR) spectroscopy, Ultra Violet/Visible (UV/Vis) spectroscopy, Fourier transfer infrared resonance (FTIR) and electron energy loss spectroscopy (EELS) to confirm the structure of the synthesis products. NMR spectroscopy confirmed the imide linkage of Schiff-base formation as two symmetrical peaks at 8.1 and 7.7 ppm respectively. Comparison of starting materials and product spectra by UV/Vis spectroscopy confirmed the disappearance of the diaminonaphthalene peak at 250 nm as evidence of complete conversion to product. FTIR spectroscopy of the Schiff-base ligand confirmed the formation of the imine bond at 1595 cm-1. EELS spectra comparing FeCl2?4H2O and the Fe-Schiff-base complex, showed good agreement in the energy loss profiles associated with changes to the electronic arrangement of Fe d-orbitals. EDS clearly identified a spectral band for Fe (7 - 8 eV) in the Fe-Schiff-base complex. Electrochemical evaluation of the Fe-Schiff-base complex was compared to the electrochemical signature of denatured cytochrome-C using cyclic voltammetry and square wave voltammetry. The Fe2+/Fe3+ quasi-reversible behavior for iron in the metallated complex was observed at -0.430 V vs. Ag/AgCl, which is consistent with reference values for iron in macromolecular structures.
文摘A method using synchrotron radiation X-ray fluorescence analysis to in situ determine trace elements during protein electrophoretically separating process was established. The distribution of elements in protein bands for human liver cytosolic sample separated by SDS-PAGE was analyzed a-long polyacrylamide gel. The results showed that the protein fraction of peak III in cvtosol was mainly composed of metal ion Zn-associated proteins, being in agreement with that given by atomic absorption spectrometry. Thus, it demonstrated the feasibility of this novel technique for in situ analysis of trace elements in protein bands.
文摘Cells contain a large number of metalloproteins that commonly harbor at least one metal ion cofactor. In metalloproteins, metal ions are usually coordinated by oxygen, sulfur, or nitrogen centers belonging to amino acid residues in the protein. The presence of the metal ion in metalloproteins allows them to take part in diverse biological processes, such as genome stability, metabolic catalysis, and cell cycle progression. Clinically, alteration of the function of metalloproteins in mammals is genetically associated with diseases characterized by DNA damage and repair defects. The present review focuses on the current perspectives of metal ion homeostasis in different organisms and summarizes the most recent understanding on magnesium, copper, iron, and manganese-containing proteins and their functional involvement in the maintenance of genome stability.
文摘In this study, we exhibited an amino acid (arginine and threonine) derivative Schiff base copper(II) complexes incorporating an azobenzene moiety as a photoresponsive site and conjugated it to egg white lysozyme, a well-known protein, to change ligand conformation under binding to lysozyme. Among several spectroscopic investigations, ESR clearly showed that the nitrogen atom of the amino acid residue of lysozyme was bound to the paramagnetic copper(II) ion of the complex, and UV light irradiation confirmed photoisomerization of the azobenzene moiety of the ligand to cis-form. The binding mode was considered by means of spectroscopic as well as computational methods, whereas complete crystallographic verification was still a preliminary stage.
基金This work was supported by the National Natural Science Foundation of China(No.22173032 and No.21933010).We also acknowledge the support of NYU Shanghai and ECNU Multifunctional Platform for Innovation(No.001).
文摘Objective: To study the effects of operation combined with chemotherapy on MMP-2, TIMP-1, VEGF, NSE, LDH and immunologic function in children with neuroblastoma (NB). Methods: A total of 90 children with NB in our hospital from January 2014 to January 2017 were enrolled in this study. The subjects were divided into the control group (n=45) and the treatment group (n=45) randomly. The control group was treated with operation for 7 periods;the treatment group was treated with operation combined with chemotherapy for 7 periods. The serum MMP-2, TIMP-1, VEGF, NSE, LDH, IgG, IgA, IgM, IgE and peripheral blood TTL, THL, TSL, NK, CD4+/CD8+ of the two groups before and after treatment were compared. Results: There were no significantly differences of the serum MMP-2, TIMP-1, VEGF, NSE, LDH of the two groups before treatment. The serum MMP-2, TIMP-1, VEGF, NSE, LDH of the two groups after treatment were significantly lower than before treatment, and that of the treatment group after treatment were significantly lower than the control group. There were no significantly differences of the serum IgG, IgA, IgM, IgE of the two groups before treatment. The serum IgG, IgA, IgM, IgE of the two groups after treatment were significantly lower than before treatment, and that of the treatment group after treatment were significantly lower than the control group. There were no significantly differences of the peripheral blood TTL, THL, TSL, NK, CD4+/CD8+ of the two groups before treatment. The peripheral blood TTL, CD4+/CD8+ of the two groups after treatment were significantly lower than before treatment, the peripheral blood THL, TSL, NK of the two groups after treatment were significantly higher than before treatment, and that of the treatment group after treatment were significantly better than the control group. Conclusion: Operation combined with chemotherapy can inhabit the tumor infiltration and metastasis of the children with NB, can improve the cellular immunity function, and it was worthy clinical application.
