创伤弧菌是一种重要的人鱼共患致病菌,根据目前已报道的致病因子,产铁载体是其主要的致病因子之一。以海水中筛选分离出的创伤弧菌为实验菌株,通过改良金氏培养基(Modified King B,MKB)筛选产铁载体菌株,通过平板覆盖法筛选高产铁载体...创伤弧菌是一种重要的人鱼共患致病菌,根据目前已报道的致病因子,产铁载体是其主要的致病因子之一。以海水中筛选分离出的创伤弧菌为实验菌株,通过改良金氏培养基(Modified King B,MKB)筛选产铁载体菌株,通过平板覆盖法筛选高产铁载体菌株及紫外可见分光光度计法测定铁载体活性单位(siderphore units,SU)。基于MKB培养基,选取产铁载体能力最高的V.vulnificus Y8菌株,利用响应面法对其铁载体活性单位的显著影响因素包括初始p H、装液量及碳氮比(C/N)进行优化。响应面优化结果表明:在初始p H7.84、装液量81.53 m L及碳氮比(C/N)3.06的条件下,铁载体活性单位预测值为90.7404%,并通过三次平行验证实验证明,实际平均SU值和预测SU值接近,较优化前提高了23.73%。响应面优化了MKB诱导产铁载体条件,实现铁载体的高效诱导,为日后的进一步研究奠定基础。展开更多
This review discusses the recent reports on one-dimensional(1D) nanostructures with unusual flexibility.We discuss the importance that flexibility could have in future applications of nanowires and other nanostructure...This review discusses the recent reports on one-dimensional(1D) nanostructures with unusual flexibility.We discuss the importance that flexibility could have in future applications of nanowires and other nanostructures,and detail the two main approaches that have been followed to this day to synthesize highly flexible 1D nanostructures.One approach is based on making crystals in which one or two dimensions of the structure are comparable in size with the unit cell.Such thinness has been shown to provide unusual flexibility.The other approach conjoins hard nanostructures with flexible joints.展开更多
文摘创伤弧菌是一种重要的人鱼共患致病菌,根据目前已报道的致病因子,产铁载体是其主要的致病因子之一。以海水中筛选分离出的创伤弧菌为实验菌株,通过改良金氏培养基(Modified King B,MKB)筛选产铁载体菌株,通过平板覆盖法筛选高产铁载体菌株及紫外可见分光光度计法测定铁载体活性单位(siderphore units,SU)。基于MKB培养基,选取产铁载体能力最高的V.vulnificus Y8菌株,利用响应面法对其铁载体活性单位的显著影响因素包括初始p H、装液量及碳氮比(C/N)进行优化。响应面优化结果表明:在初始p H7.84、装液量81.53 m L及碳氮比(C/N)3.06的条件下,铁载体活性单位预测值为90.7404%,并通过三次平行验证实验证明,实际平均SU值和预测SU值接近,较优化前提高了23.73%。响应面优化了MKB诱导产铁载体条件,实现铁载体的高效诱导,为日后的进一步研究奠定基础。
基金supported by Iowa State University of Science and Technology through startup funds
文摘This review discusses the recent reports on one-dimensional(1D) nanostructures with unusual flexibility.We discuss the importance that flexibility could have in future applications of nanowires and other nanostructures,and detail the two main approaches that have been followed to this day to synthesize highly flexible 1D nanostructures.One approach is based on making crystals in which one or two dimensions of the structure are comparable in size with the unit cell.Such thinness has been shown to provide unusual flexibility.The other approach conjoins hard nanostructures with flexible joints.