BACKGROUND:A growing body of evidence suggests that many tumors are initiated by both epigenetic abnormalities and gene mutations,which promote tumor progression. Epigenetic abnormalities include changes in DNA methyl...BACKGROUND:A growing body of evidence suggests that many tumors are initiated by both epigenetic abnormalities and gene mutations,which promote tumor progression. Epigenetic abnormalities include changes in DNA methylation and in the modification of histones.This study aimed to assess the status of methylation in the CpG island(CGI)of the tumor necrosis factor receptor superfamily member 10c(TNFRSF10C) with combined bisulfite restriction analysis(COBRA)and to evaluate its role in the progression of pancreatic cancer(PC). METHODS:The methylation status of four PC cell lines was assessed using COBRA and/or bisulfite genomic sequencing (BGS).Changes in methylation and TNFRSF10C expression in PC cell lines before and after treatment with 5-aza-2’-deoxycytidine(5-aza-dC)and/or trichostatin A(TSA)were assessed by BGS and real-time RT-PCR.Apoptosis in the four cell lines was tested by flow cytometry(FCM)and TUNEL assay. RESULTS:The methylation status of the TNFRSF10C promoter was assessed in PC cells(BxPC-3:68.84±8.71%;CFPAC-1:0; PANC-1:96.77±4.57%;SW1990:54.97±7.33%)with the COBRA assay,which was confirmed by the results of BGS.After treatment with 5-aza-dC and/or TSA,apoptosis was induced in PC cells to different degrees,and the levels of TNFRSF10C transcriptional expression in the PC cell lines(except CFPAC-1) increased markedly after 5-aza-dC treatment. CONCLUSIONS:A high frequency of CGI methylation in the TNFRSF10C promoter results in inactivation of the gene and enhancement of tumor growth in most PC cell lines(except CFPAC-1).Inactivation of TNFRSF10C by CGI hypermethylation can play an important role in PC progression and be potentially useful as a diagnostic marker and a new therapeutic approach for PC.展开更多
The efficient cycloaddition reactions were carried out to afford the novel 3,5-disubstituted-dihydroisoxazoles from methyl undec-10-enoate and isoxazoles, triazole and tetrazolo-triazole from methyl undec-10-ynoate. T...The efficient cycloaddition reactions were carried out to afford the novel 3,5-disubstituted-dihydroisoxazoles from methyl undec-10-enoate and isoxazoles, triazole and tetrazolo-triazole from methyl undec-10-ynoate. The reactions occurred under relatively mild conditions and afforded the desired products in good yields.展开更多
Diphenyl-2, 2-dicyanoethylene reacts with 10-methyl-9, 10-dihydroacridine in deaerated acetonitrile under irradiation with l>320 nm to give the coupling product 1, 1-diphenyl-1-(10-methyl-9-acridinyl)-2, 2-dicyanoe...Diphenyl-2, 2-dicyanoethylene reacts with 10-methyl-9, 10-dihydroacridine in deaerated acetonitrile under irradiation with l>320 nm to give the coupling product 1, 1-diphenyl-1-(10-methyl-9-acridinyl)-2, 2-dicyanoethane, which has been characterized by X-ray crystallographic, MS and NMR analyses.展开更多
目的:研究结肠癌组织白细胞介素-10(IL-10)和干扰素-γ(IFN-γ)的基因表达及其相关的启动子区甲基化状态。方法:随机选取2011年1月-2014年6月80例结肠癌患者组织为结肠癌组、80例相应癌旁组织为癌旁组和100例正常健康者结肠组织为对照...目的:研究结肠癌组织白细胞介素-10(IL-10)和干扰素-γ(IFN-γ)的基因表达及其相关的启动子区甲基化状态。方法:随机选取2011年1月-2014年6月80例结肠癌患者组织为结肠癌组、80例相应癌旁组织为癌旁组和100例正常健康者结肠组织为对照组。采用亚硫酸氢盐测序法、甲基化特异性PCR和实时荧光定量PCR检测对IL-10和IFN-γ基因的甲基化状况进行检测,分析其与结肠癌主要临床特征之间的关系。结果:三组组织的IL-10和IFN-γm RNA水平之间比较,差异均有统计学意义(P<0.05),其中结肠癌组和癌旁组组织的IL-10 m RNA均高于对照组(P<0.05),结肠癌组和癌旁组组织的IFN-γm RNA均低于对照组(P<0.05);三组IL-10基因启动子区-110,-185位点甲基化率比较,差异均有有统计学意义(P<0.05),其中结肠癌组和癌旁组组织的IL-10基因启动子区-110均高于对照组(P<0.05),结肠癌组组织的IL-10基因启动子区-185低于对照组(P<0.05);IFN-γ基因启动子区甲基化率结肠癌组>癌旁组>对照组,差异均有统计学意义(P<0.05)。结论:IL-10和IFN-γ基因启动子区甲基化状态影响其在结肠癌组织中的表达,结肠癌患者处于TH2极化状态,与结肠癌发生发展密切相关。展开更多
Efficient composite semiconductor photocatalysts are highly desirable for the visible-light-driven degradation of organic pollutants. In this study, Bi24O31Cl10 photocatalyst was prepared via a hydrothermal method and...Efficient composite semiconductor photocatalysts are highly desirable for the visible-light-driven degradation of organic pollutants. In this study, Bi24O31Cl10 photocatalyst was prepared via a hydrothermal method and modified with Pt nanoparticles (NPs) through a facile deposition procedure. The composite photocatalyst was characterized by X-ray diffraction, transmission electronic microscopy, X-ray photoelectron spectroscopy, UV-vis diffusion reflectance spectroscopy, photoluminescence spectroscopy, and electron spin resonance. The 1.0 wt% Pt/Bi24O31Cl10 photocatalyst showed the highest activity for the degradation of methyl orange under visible light (source: 300 W Xe lamp coupled with a UV-cutoff filter), and the photocatalytic degradation efficiency improved about 2.2 times compared to that of pure Bi24O31Cl10. The composite photocatalyst could maintain most of its activity after four runs of the photocatalytic experimental cycle. This study could provide a novel insight for the modification of other desirable semiconductor materials to achieve high photocatalytic activities.展开更多
基金supported by a grant from the National Natural Science Foundation of China(30471691)
文摘BACKGROUND:A growing body of evidence suggests that many tumors are initiated by both epigenetic abnormalities and gene mutations,which promote tumor progression. Epigenetic abnormalities include changes in DNA methylation and in the modification of histones.This study aimed to assess the status of methylation in the CpG island(CGI)of the tumor necrosis factor receptor superfamily member 10c(TNFRSF10C) with combined bisulfite restriction analysis(COBRA)and to evaluate its role in the progression of pancreatic cancer(PC). METHODS:The methylation status of four PC cell lines was assessed using COBRA and/or bisulfite genomic sequencing (BGS).Changes in methylation and TNFRSF10C expression in PC cell lines before and after treatment with 5-aza-2’-deoxycytidine(5-aza-dC)and/or trichostatin A(TSA)were assessed by BGS and real-time RT-PCR.Apoptosis in the four cell lines was tested by flow cytometry(FCM)and TUNEL assay. RESULTS:The methylation status of the TNFRSF10C promoter was assessed in PC cells(BxPC-3:68.84±8.71%;CFPAC-1:0; PANC-1:96.77±4.57%;SW1990:54.97±7.33%)with the COBRA assay,which was confirmed by the results of BGS.After treatment with 5-aza-dC and/or TSA,apoptosis was induced in PC cells to different degrees,and the levels of TNFRSF10C transcriptional expression in the PC cell lines(except CFPAC-1) increased markedly after 5-aza-dC treatment. CONCLUSIONS:A high frequency of CGI methylation in the TNFRSF10C promoter results in inactivation of the gene and enhancement of tumor growth in most PC cell lines(except CFPAC-1).Inactivation of TNFRSF10C by CGI hypermethylation can play an important role in PC progression and be potentially useful as a diagnostic marker and a new therapeutic approach for PC.
文摘The efficient cycloaddition reactions were carried out to afford the novel 3,5-disubstituted-dihydroisoxazoles from methyl undec-10-enoate and isoxazoles, triazole and tetrazolo-triazole from methyl undec-10-ynoate. The reactions occurred under relatively mild conditions and afforded the desired products in good yields.
文摘Diphenyl-2, 2-dicyanoethylene reacts with 10-methyl-9, 10-dihydroacridine in deaerated acetonitrile under irradiation with l>320 nm to give the coupling product 1, 1-diphenyl-1-(10-methyl-9-acridinyl)-2, 2-dicyanoethane, which has been characterized by X-ray crystallographic, MS and NMR analyses.
文摘目的:研究结肠癌组织白细胞介素-10(IL-10)和干扰素-γ(IFN-γ)的基因表达及其相关的启动子区甲基化状态。方法:随机选取2011年1月-2014年6月80例结肠癌患者组织为结肠癌组、80例相应癌旁组织为癌旁组和100例正常健康者结肠组织为对照组。采用亚硫酸氢盐测序法、甲基化特异性PCR和实时荧光定量PCR检测对IL-10和IFN-γ基因的甲基化状况进行检测,分析其与结肠癌主要临床特征之间的关系。结果:三组组织的IL-10和IFN-γm RNA水平之间比较,差异均有统计学意义(P<0.05),其中结肠癌组和癌旁组组织的IL-10 m RNA均高于对照组(P<0.05),结肠癌组和癌旁组组织的IFN-γm RNA均低于对照组(P<0.05);三组IL-10基因启动子区-110,-185位点甲基化率比较,差异均有有统计学意义(P<0.05),其中结肠癌组和癌旁组组织的IL-10基因启动子区-110均高于对照组(P<0.05),结肠癌组组织的IL-10基因启动子区-185低于对照组(P<0.05);IFN-γ基因启动子区甲基化率结肠癌组>癌旁组>对照组,差异均有统计学意义(P<0.05)。结论:IL-10和IFN-γ基因启动子区甲基化状态影响其在结肠癌组织中的表达,结肠癌患者处于TH2极化状态,与结肠癌发生发展密切相关。
基金supported by the National Natural Science Foundation of China(51572295,21273285 and 21003157)Beijing Nova Program(2008B76)Science Foundation of China University of Petroleum Beijing(KYJJ2012-06-20 and 2462016YXBS05)~~
文摘Efficient composite semiconductor photocatalysts are highly desirable for the visible-light-driven degradation of organic pollutants. In this study, Bi24O31Cl10 photocatalyst was prepared via a hydrothermal method and modified with Pt nanoparticles (NPs) through a facile deposition procedure. The composite photocatalyst was characterized by X-ray diffraction, transmission electronic microscopy, X-ray photoelectron spectroscopy, UV-vis diffusion reflectance spectroscopy, photoluminescence spectroscopy, and electron spin resonance. The 1.0 wt% Pt/Bi24O31Cl10 photocatalyst showed the highest activity for the degradation of methyl orange under visible light (source: 300 W Xe lamp coupled with a UV-cutoff filter), and the photocatalytic degradation efficiency improved about 2.2 times compared to that of pure Bi24O31Cl10. The composite photocatalyst could maintain most of its activity after four runs of the photocatalytic experimental cycle. This study could provide a novel insight for the modification of other desirable semiconductor materials to achieve high photocatalytic activities.
