The interaction of Methylthymol Blue(MTB)-Zinc! compound and Alizarin Red S(ARS)-Aluminum" compound with Bovine serum albumin (BSA) was investigated by UV-Vis spectrophotometric method in acidic buffer solution. ...The interaction of Methylthymol Blue(MTB)-Zinc! compound and Alizarin Red S(ARS)-Aluminum" compound with Bovine serum albumin (BSA) was investigated by UV-Vis spectrophotometric method in acidic buffer solution. MTB-Zn!-BSA was a blue color compound, which possesses maximum absorption at 613 nm with 172 nm, 174 nm and 18 nm of red shift compared to the MTB, MTB-BSA and MTB-Zn! complexes respectively. Dual wavelength substantial amount ratio method, balance dialysis substantial amount ratio method and unity wavelength substantial amount ratio method were compared. The following results were obtained: the apparent molar absorptivity of MTB-Zn! with BSA was ε=2.20×104 L·mol-1·cm-1. Conditional proportion were defined, nMTB∶nZn!∶nBSA=2∶2∶1; condition combination constant, K=2.07×1010. Combination proportion were defined, nARS∶nAl"∶ nBSA=6∶4∶1. Condition equilibrium constant of reaction of ARS-Al" with BSA was K=8.80×108. The apparent mo- lar absorptivity of ε=2.65×104 L·mol-·1cm-1. It is suggested that combination between BSA and MTB-Zn" is due to coordination force. That combination between BSA and ARS-Al" is due to the coordination bond and electro- static force.展开更多
采用荧光光谱、紫外-可见光谱研究了有/无金属Zn2+存在下甲基百里酚蓝(MTB)与牛血清白蛋白(BSA)的相互作用.实验结果表明,无论Zn2+离子存在与否,MTB与BSA之间均为一形成复合物的静态猝灭过程.根据Stern-Volmer方程和Lineweaver-Burk方...采用荧光光谱、紫外-可见光谱研究了有/无金属Zn2+存在下甲基百里酚蓝(MTB)与牛血清白蛋白(BSA)的相互作用.实验结果表明,无论Zn2+离子存在与否,MTB与BSA之间均为一形成复合物的静态猝灭过程.根据Stern-Volmer方程和Lineweaver-Burk方程求出了其结合常数与热力学参数,发现Zn2+离子存在时,MTB与BSA间的作用力由静电力转为氢键和Van der Waals力作用为主,认为金属Zn2+以"离子架桥"的方式参与MTB与BSA的结合过程,从而ΔH对ΔG的贡献增大.展开更多
文摘The interaction of Methylthymol Blue(MTB)-Zinc! compound and Alizarin Red S(ARS)-Aluminum" compound with Bovine serum albumin (BSA) was investigated by UV-Vis spectrophotometric method in acidic buffer solution. MTB-Zn!-BSA was a blue color compound, which possesses maximum absorption at 613 nm with 172 nm, 174 nm and 18 nm of red shift compared to the MTB, MTB-BSA and MTB-Zn! complexes respectively. Dual wavelength substantial amount ratio method, balance dialysis substantial amount ratio method and unity wavelength substantial amount ratio method were compared. The following results were obtained: the apparent molar absorptivity of MTB-Zn! with BSA was ε=2.20×104 L·mol-1·cm-1. Conditional proportion were defined, nMTB∶nZn!∶nBSA=2∶2∶1; condition combination constant, K=2.07×1010. Combination proportion were defined, nARS∶nAl"∶ nBSA=6∶4∶1. Condition equilibrium constant of reaction of ARS-Al" with BSA was K=8.80×108. The apparent mo- lar absorptivity of ε=2.65×104 L·mol-·1cm-1. It is suggested that combination between BSA and MTB-Zn" is due to coordination force. That combination between BSA and ARS-Al" is due to the coordination bond and electro- static force.
文摘采用荧光光谱、紫外-可见光谱研究了有/无金属Zn2+存在下甲基百里酚蓝(MTB)与牛血清白蛋白(BSA)的相互作用.实验结果表明,无论Zn2+离子存在与否,MTB与BSA之间均为一形成复合物的静态猝灭过程.根据Stern-Volmer方程和Lineweaver-Burk方程求出了其结合常数与热力学参数,发现Zn2+离子存在时,MTB与BSA间的作用力由静电力转为氢键和Van der Waals力作用为主,认为金属Zn2+以"离子架桥"的方式参与MTB与BSA的结合过程,从而ΔH对ΔG的贡献增大.
