Objective: This study was to investigate the role of hsa-miR-155-3p and hsa-miR-155-5p as biomarkers and regulators of biological behavior in Systemic Sclerosis. Methods: A total of 10 SSc patients and 10 healthy cont...Objective: This study was to investigate the role of hsa-miR-155-3p and hsa-miR-155-5p as biomarkers and regulators of biological behavior in Systemic Sclerosis. Methods: A total of 10 SSc patients and 10 healthy controls were selected for the study. The expression levels of hsa-miR-155-3p and hsa-miR-155-5p in peripheral blood mononuclear cells of SSc patients and healthy controls were measured using RT-qPCR. The diagnostic value of these miRNAs was explored using Receiver Operating Characteristic curve analysis. Pearson or Spearman correlation analysis was performed to assess the correlation between miRNAs and clinical indicators in SSc patients. Potential target genes of hsa-miR-155-3p and hsa-miR-155-5p were predicted using miRDB, Targetscan, and miRDIP databases. GO functional annotation, KEGG pathway enrichment analysis, protein-protein interaction network construction, and selection of central genes were conducted. Results: The expression levels of hsa-miR-155-3p and hsa- miR-155-5p were significantly higher in PBMCs of SSc patients compared to healthy controls (P<0.001). The ROC curve analysis showed that hsa-miR-155-3p and hsa-miR-155-5p had a high diagnostic value for SSc (AUC=1, P<0.001). Correlation analysis revealed that hsa- miR-155-3p, hsa-miR-155-5p, and clinical indicators such as high-resolution CT, neutrophil percentage, lymphocyte percentage, and albumin to globulin ratio were correlated (P<0.05). The signaling pathways enriched with target genes of hsa-miR-155-3p and hsa-miR-155- 5p were closely associated with the occurrence and development of SSc fibrosis, immunity, and inflammation. Conclusions: hsa-miR-155-3p and hsa-miR-155-5p may be involved in regulating the occurrence and development of SSc fibrosis, immunity, and inflammation. They have the potential to serve as biomarkers for clinical diagnosis and treatment of SSc.展开更多
类风湿关节炎(rheumatoid arthritis,RA)是以滑膜炎为主要病变的自身免疫疾病,持续的滑膜炎症是介导血管翳生成及软骨破坏的关键。miR-155与Toll样受体(toll like receptor,TLR)4/核因子(nuclear factor,NF)-κB通路在RA滑膜组织中高表...类风湿关节炎(rheumatoid arthritis,RA)是以滑膜炎为主要病变的自身免疫疾病,持续的滑膜炎症是介导血管翳生成及软骨破坏的关键。miR-155与Toll样受体(toll like receptor,TLR)4/核因子(nuclear factor,NF)-κB通路在RA滑膜组织中高表达,可促进RA滑膜炎症反应。miR-155可促进数种免疫细胞活化并介导炎症反应,同时可以通过激活TLR4/NF-κB通路转录多种炎症因子及活化因子。中医治疗RA有一定的优势,大量研究证实中药、方剂及针灸等治疗可抑制RA炎症反应,其作用机制与调节miR-155及TLR4/NF-κB通路相关。免疫调控是中医整体观念的体现,miR-155/TLR4/NF-κB信号轴在该方面的研究中仍是热点,本文述评了中医药通过该信号轴调控免疫活性并抑制RA炎症的研究现状,以期为今后研究提供思路。展开更多
基金National Natural Science Foundation of China(No.8186029481860295)Natural Science Foundation of Inner Mongolia Autonomous Region(No.2019MS080552021MS08045)Science and Technology Plan Project of Inner Mongolia Autonomous Region(No.2018020892019GG052)。
文摘Objective: This study was to investigate the role of hsa-miR-155-3p and hsa-miR-155-5p as biomarkers and regulators of biological behavior in Systemic Sclerosis. Methods: A total of 10 SSc patients and 10 healthy controls were selected for the study. The expression levels of hsa-miR-155-3p and hsa-miR-155-5p in peripheral blood mononuclear cells of SSc patients and healthy controls were measured using RT-qPCR. The diagnostic value of these miRNAs was explored using Receiver Operating Characteristic curve analysis. Pearson or Spearman correlation analysis was performed to assess the correlation between miRNAs and clinical indicators in SSc patients. Potential target genes of hsa-miR-155-3p and hsa-miR-155-5p were predicted using miRDB, Targetscan, and miRDIP databases. GO functional annotation, KEGG pathway enrichment analysis, protein-protein interaction network construction, and selection of central genes were conducted. Results: The expression levels of hsa-miR-155-3p and hsa- miR-155-5p were significantly higher in PBMCs of SSc patients compared to healthy controls (P<0.001). The ROC curve analysis showed that hsa-miR-155-3p and hsa-miR-155-5p had a high diagnostic value for SSc (AUC=1, P<0.001). Correlation analysis revealed that hsa- miR-155-3p, hsa-miR-155-5p, and clinical indicators such as high-resolution CT, neutrophil percentage, lymphocyte percentage, and albumin to globulin ratio were correlated (P<0.05). The signaling pathways enriched with target genes of hsa-miR-155-3p and hsa-miR-155- 5p were closely associated with the occurrence and development of SSc fibrosis, immunity, and inflammation. Conclusions: hsa-miR-155-3p and hsa-miR-155-5p may be involved in regulating the occurrence and development of SSc fibrosis, immunity, and inflammation. They have the potential to serve as biomarkers for clinical diagnosis and treatment of SSc.
文摘类风湿关节炎(rheumatoid arthritis,RA)是以滑膜炎为主要病变的自身免疫疾病,持续的滑膜炎症是介导血管翳生成及软骨破坏的关键。miR-155与Toll样受体(toll like receptor,TLR)4/核因子(nuclear factor,NF)-κB通路在RA滑膜组织中高表达,可促进RA滑膜炎症反应。miR-155可促进数种免疫细胞活化并介导炎症反应,同时可以通过激活TLR4/NF-κB通路转录多种炎症因子及活化因子。中医治疗RA有一定的优势,大量研究证实中药、方剂及针灸等治疗可抑制RA炎症反应,其作用机制与调节miR-155及TLR4/NF-κB通路相关。免疫调控是中医整体观念的体现,miR-155/TLR4/NF-κB信号轴在该方面的研究中仍是热点,本文述评了中医药通过该信号轴调控免疫活性并抑制RA炎症的研究现状,以期为今后研究提供思路。
