AIM: To determine the expression of mi R-422 a in colorectal cancer(CRC) tissues and to further explore the prognostic value and function of mi R-422 a in CRC carcinogenesis.METHODS: mi R-422 a expression was analyzed...AIM: To determine the expression of mi R-422 a in colorectal cancer(CRC) tissues and to further explore the prognostic value and function of mi R-422 a in CRC carcinogenesis.METHODS: mi R-422 a expression was analyzed in 102 CRC tissues and paired normal mucosa adjacent to carcinoma by quantitative real-time PCR. The relationship of mi R-422 a expression with clinicopathological parameters was also analyzed. Kaplan-Meier analysis and Cox multivariate analysis were performed to estimate the potential role of mi R-422 a. Cell proliferation, migration, and invasion were used for in vitro functional analysis of mi R-422 a.RESULTS: The levels of mi R-422 a were dramatically reduced in CRC tissues compared with normal mucosa(P < 0.05), and significantly correlated with local invasion(P = 0.004) and lymph node metastasis(P < 0.001). Kaplan-Meier survival and Cox regression multivariate analyses revealed that mi R-422 a expression(HR = 0.568, P = 0.015) and clinical TNM stage(HR = 2.942, P = 0.003) were independent prognostic factorsfor overall survival in CRC patients. Furthermore, in vitro experiments showed that overexpression of mi R-422 a inhibited the proliferation, migration, and invasion of SW480 and HT-29 cells.CONCLUSION: Down-regulation of mi R-422 a may serve as an independent prognosis factor in CRC. Mi R-422 a functions as a tumor suppressor and regulates progression of CRC.展开更多
目的探讨创伤性颅脑损伤患者血清miR-422a,miR-212-5p表达与病情和预后的相关性,分析miR-422a,miR-212-5p诊断创伤性脑损伤患者预后的价值。方法连续性选择2018年4月~2020年6月山东省菏泽市中医院收治的173例创伤性颅脑损伤患者(创伤组...目的探讨创伤性颅脑损伤患者血清miR-422a,miR-212-5p表达与病情和预后的相关性,分析miR-422a,miR-212-5p诊断创伤性脑损伤患者预后的价值。方法连续性选择2018年4月~2020年6月山东省菏泽市中医院收治的173例创伤性颅脑损伤患者(创伤组)和同期125例志愿者(对照组)。根据格拉斯哥昏迷量表(GCS)评分将创伤组患者分为轻度组(GCS评分13~15分,57例),中度组(GCS评分9~12分,63例)和重度组(GCS评分3~8分,53例),追踪临床结局,根据格拉斯哥预后量表(GOS)评分将患者分为预后不良组(GOS评分1~3分,62例)和预后良好组(GOS评分4~5分,111例)。检测所有受试者血清miR-422a,miR-212-5p表达,比较组间差异,分析miR-422a,miR-212-5p与创伤性颅脑损伤患者预后的关系以及miR-422a,miR-212-5p预测患者预后的价值。结果创伤组血清miR-422a表达高于对照组(3.02±1.02 vs 0.95±0.21),miR-212-5p表达低于对照组(1.03±0.28 vs 2.85±0.61),差异均有统计学意义(t=22.340,34.544,均P<0.05)。重度组血清miR-422a表达高于中度组和轻度组(4.01±0.13 vs 2.92±0.66,2.21±0.12;t=11.824,75.516,均P<0.05),miR-212-5p表达低于中度组和轻度组(0.84±0.06 vs 0.97±0.25,1.27±0.04;t=3.695,44.512,均P<0.05),预后不良组血清miR-422a表达高于预后良好组(4.05±0.11 vs 2.44±0.31,t=22.340,P<0.05),miR-212-5p表达低于预后良好组(0.83±0.06 vs 1.14±0.21,t=22.340,P<0.05),差异均有统计学意义。低GCS评分(OR=0.825,95%CI:0.721~0.945),高miR-422a表达(OR=1.394,95%CI:1.194~1.627),低miR-212-5p表达(OR=0.744,95%CI:0.667~0.831)是创伤性颅脑损伤患者预后不良的危险因素(均P<0.05)。联合miR-422a,miR-212-5p预测创伤性颅脑损伤患者预后不良的曲线下面积为0.907,高于单独检测miR-422a,miR-212-5p的0.797,0.775(Z=2.412,2.561,均P<0.05)。结论miR-422a过表达和miR-212-5p表达缺失与创伤性脑损伤严重程度和不良预后有关,可作为预后预测的潜在生物学指标。展开更多
Objective To explore the potential predictive value of plasma exosomal miR-422a in different time after ischaemic stroke(1,2,3,4 day).Methods Forty patients diagnosed with ischaemic stroke(IS)and ten age and sex match...Objective To explore the potential predictive value of plasma exosomal miR-422a in different time after ischaemic stroke(1,2,3,4 day).Methods Forty patients diagnosed with ischaemic stroke(IS)and ten age and sex matched people who underwent a standard physical examination were recruited from the First Affiliated Hospital of Guangxi Medical University between May 2016 and December 2016.Plasma exosomes were extracted by use of related kit and the expression level of plasma exosomal miR-422a in both IS patients(1,2,3,4 day)and healthy controls were examined via quantitative real-time polymerase chain reaction(qRT-PCR).The areas under the curve(AUC)of the receiver operating characteristic curve were constructed to evaluate the diagnostic accuracy of the miR-422a in IS,and one-way analysis of variance followed by the Games-Howell post hoc test was used for difference analysis.Results The exosomes isolated from human plasma showed round or oval vesicles,the average particle size was 128.2 nm and with maximum peak distribution of 186.9 nm.Moreover,all of the isolated exosomes were positive for a marker,with the CD63-positive rate at 80.6% and the CD81-positive rate at 91.7%.qRT-PCR confirmed that miR-422a was expressed in human plasma exosomes,and the expression levels of plasma exosomal miR-422a [median relative values,1.221(95% CI:0.640-1.802)for the healthy group,4.418(95% CI:2.642-6.193)for group of 1day after IS,2.912(95% CI:2.262-3.562)for 2 day,2.744(95% CI:2.000-3.487)for 3 day and 0.449(95% CI:0.170-0.727)for 4 day] were significantly increased in the time after IS 1,2,3 day(F=13.57,P<0.05,P<0.01,P<0.05,respectively;and the AUC values were 0.920,0.945,0.870,respectively).The expression of plasma exosomal miR-422a on the 4th day after IS were lower than those in other IS groups(F=13.57,P<0.005,P<0.001,P<0.001,respectively),and no statistical significance was found between the expression of plasma exosomal miR-422a on the 4th day after IS than that in the control group [0.449(95% CI:0.170-0.727)].Conclusion Plasma exosomal miR-422a showed a high diagnostic value as blood-based biomarker for diagnosing IS in the early phase(1-3 d).展开更多
基金Supported by the National Natural Science Foundation of China,No.81472025Outstanding Young Scientist Research Award Fund of Shandong Province,No.BS2014YY023+3 种基金Projects of Medical and Health Technology Development Program of Shandong Province,No.2014WS0124Science Foundation of Qilu Hospital of Shandong University,No.2015QLQN37Fundamental Research of Shandong Universitythe National Key Clinical Medical Specialties Foundation
文摘AIM: To determine the expression of mi R-422 a in colorectal cancer(CRC) tissues and to further explore the prognostic value and function of mi R-422 a in CRC carcinogenesis.METHODS: mi R-422 a expression was analyzed in 102 CRC tissues and paired normal mucosa adjacent to carcinoma by quantitative real-time PCR. The relationship of mi R-422 a expression with clinicopathological parameters was also analyzed. Kaplan-Meier analysis and Cox multivariate analysis were performed to estimate the potential role of mi R-422 a. Cell proliferation, migration, and invasion were used for in vitro functional analysis of mi R-422 a.RESULTS: The levels of mi R-422 a were dramatically reduced in CRC tissues compared with normal mucosa(P < 0.05), and significantly correlated with local invasion(P = 0.004) and lymph node metastasis(P < 0.001). Kaplan-Meier survival and Cox regression multivariate analyses revealed that mi R-422 a expression(HR = 0.568, P = 0.015) and clinical TNM stage(HR = 2.942, P = 0.003) were independent prognostic factorsfor overall survival in CRC patients. Furthermore, in vitro experiments showed that overexpression of mi R-422 a inhibited the proliferation, migration, and invasion of SW480 and HT-29 cells.CONCLUSION: Down-regulation of mi R-422 a may serve as an independent prognosis factor in CRC. Mi R-422 a functions as a tumor suppressor and regulates progression of CRC.
文摘目的探讨创伤性颅脑损伤患者血清miR-422a,miR-212-5p表达与病情和预后的相关性,分析miR-422a,miR-212-5p诊断创伤性脑损伤患者预后的价值。方法连续性选择2018年4月~2020年6月山东省菏泽市中医院收治的173例创伤性颅脑损伤患者(创伤组)和同期125例志愿者(对照组)。根据格拉斯哥昏迷量表(GCS)评分将创伤组患者分为轻度组(GCS评分13~15分,57例),中度组(GCS评分9~12分,63例)和重度组(GCS评分3~8分,53例),追踪临床结局,根据格拉斯哥预后量表(GOS)评分将患者分为预后不良组(GOS评分1~3分,62例)和预后良好组(GOS评分4~5分,111例)。检测所有受试者血清miR-422a,miR-212-5p表达,比较组间差异,分析miR-422a,miR-212-5p与创伤性颅脑损伤患者预后的关系以及miR-422a,miR-212-5p预测患者预后的价值。结果创伤组血清miR-422a表达高于对照组(3.02±1.02 vs 0.95±0.21),miR-212-5p表达低于对照组(1.03±0.28 vs 2.85±0.61),差异均有统计学意义(t=22.340,34.544,均P<0.05)。重度组血清miR-422a表达高于中度组和轻度组(4.01±0.13 vs 2.92±0.66,2.21±0.12;t=11.824,75.516,均P<0.05),miR-212-5p表达低于中度组和轻度组(0.84±0.06 vs 0.97±0.25,1.27±0.04;t=3.695,44.512,均P<0.05),预后不良组血清miR-422a表达高于预后良好组(4.05±0.11 vs 2.44±0.31,t=22.340,P<0.05),miR-212-5p表达低于预后良好组(0.83±0.06 vs 1.14±0.21,t=22.340,P<0.05),差异均有统计学意义。低GCS评分(OR=0.825,95%CI:0.721~0.945),高miR-422a表达(OR=1.394,95%CI:1.194~1.627),低miR-212-5p表达(OR=0.744,95%CI:0.667~0.831)是创伤性颅脑损伤患者预后不良的危险因素(均P<0.05)。联合miR-422a,miR-212-5p预测创伤性颅脑损伤患者预后不良的曲线下面积为0.907,高于单独检测miR-422a,miR-212-5p的0.797,0.775(Z=2.412,2.561,均P<0.05)。结论miR-422a过表达和miR-212-5p表达缺失与创伤性脑损伤严重程度和不良预后有关,可作为预后预测的潜在生物学指标。
文摘Objective To explore the potential predictive value of plasma exosomal miR-422a in different time after ischaemic stroke(1,2,3,4 day).Methods Forty patients diagnosed with ischaemic stroke(IS)and ten age and sex matched people who underwent a standard physical examination were recruited from the First Affiliated Hospital of Guangxi Medical University between May 2016 and December 2016.Plasma exosomes were extracted by use of related kit and the expression level of plasma exosomal miR-422a in both IS patients(1,2,3,4 day)and healthy controls were examined via quantitative real-time polymerase chain reaction(qRT-PCR).The areas under the curve(AUC)of the receiver operating characteristic curve were constructed to evaluate the diagnostic accuracy of the miR-422a in IS,and one-way analysis of variance followed by the Games-Howell post hoc test was used for difference analysis.Results The exosomes isolated from human plasma showed round or oval vesicles,the average particle size was 128.2 nm and with maximum peak distribution of 186.9 nm.Moreover,all of the isolated exosomes were positive for a marker,with the CD63-positive rate at 80.6% and the CD81-positive rate at 91.7%.qRT-PCR confirmed that miR-422a was expressed in human plasma exosomes,and the expression levels of plasma exosomal miR-422a [median relative values,1.221(95% CI:0.640-1.802)for the healthy group,4.418(95% CI:2.642-6.193)for group of 1day after IS,2.912(95% CI:2.262-3.562)for 2 day,2.744(95% CI:2.000-3.487)for 3 day and 0.449(95% CI:0.170-0.727)for 4 day] were significantly increased in the time after IS 1,2,3 day(F=13.57,P<0.05,P<0.01,P<0.05,respectively;and the AUC values were 0.920,0.945,0.870,respectively).The expression of plasma exosomal miR-422a on the 4th day after IS were lower than those in other IS groups(F=13.57,P<0.005,P<0.001,P<0.001,respectively),and no statistical significance was found between the expression of plasma exosomal miR-422a on the 4th day after IS than that in the control group [0.449(95% CI:0.170-0.727)].Conclusion Plasma exosomal miR-422a showed a high diagnostic value as blood-based biomarker for diagnosing IS in the early phase(1-3 d).
