目的 检测骨肉瘤血清miR-337-3p、miR-484、miR-582表达及其临床意义。方法 收集我院2016年1月至2022年1月期间收治的67例骨肉瘤患者的临床病历资料,另纳入同期60例健康志愿者作为对照组。采用DEGseq方法对血清样本进行全基因组miRNA分...目的 检测骨肉瘤血清miR-337-3p、miR-484、miR-582表达及其临床意义。方法 收集我院2016年1月至2022年1月期间收治的67例骨肉瘤患者的临床病历资料,另纳入同期60例健康志愿者作为对照组。采用DEGseq方法对血清样本进行全基因组miRNA分析,采用实时荧光定量PCR(qPCR)分析血清和组织样本中miRNA水平。受试者工作特征(ROC)曲线分析miRNA诊断骨肉瘤的效能。Logistic回归模型分析影响骨肉瘤新辅助化疗疗效的因素。Cox风险比例回归模型分析影响骨肉瘤预后的因素。结果 经全基因组miRNA分析,骨肉瘤患者血清中miR-337-3p、miR-484、miR-582、miR-1912、miR-4535的表达显著下调。qPCR检测结果显示,骨肉瘤血清miR-337-3p(0.519±0.249 vs. 1.015±0.420)、miR-484(0.669±0.254 vs. 1.029±0.515)、miR-582(0.516±0.258 vs. 0.996±0.381)均低于健康对照组(P<0.001),同时骨肉瘤组织中miR-337-3p、miR-484、miR-582相对表达量也低于癌旁正常组织(P<0.001)。骨肉瘤血清样本和组织样本中miR-337-3p、miR-484、miR-582表达量分别呈正相关(P<0.001)。ROC曲线显示血清miR-337-3p、miR-484、miR-582对于骨肉瘤有良好的诊断效能,联合曲线下面积(AUC)为0.960(95%CI:0.932~0.989)。Logistic回归分析和Cox风险比例回归模型分析均显示血清miR-337-3p、miR-484、miR-582表达水平是影响骨肉瘤新辅助化疗疗效和总生存的独立影响因素(P<0.05)。结论 血清miR-337-3p、miR-484、miR-582可作为骨肉瘤诊断、预测预后的生物标志物。展开更多
Objective:To explore the possible mechanism of Huoxue Yiqi recipe promoting angiogenesis by targeting VEGF with miR-484.Methods:The model of miR-484 overexpressed in HUVEC was prepared and intervened with Huoxue Yiqi ...Objective:To explore the possible mechanism of Huoxue Yiqi recipe promoting angiogenesis by targeting VEGF with miR-484.Methods:The model of miR-484 overexpressed in HUVEC was prepared and intervened with Huoxue Yiqi Recipe Freeze-dried powder.The effects of Huoxue Yiqi Recipe on proliferation,migration and tubular forming ability of HUVEC were separatly detected by CCK-8 method,cell scratch test and Matrigel three-dimensional forming test in vitro.The effect of Huoxue Yiqi Recipe on VEGFA mRNA expression was detected by real-time fluorescence quantitative PCR.Results:Compared with the control group,the proliferation and migration area of cells in miR-484 overexpression model group decreased,the length,number of branches and nodes involved in tube formation decreased,the tube formation ability decreased significantly,and the expression level of VEGFA mRNA decreased,with statistical significance.Compared with the model group,the cell proliferation ability,migration area,length,number of branches and nodes involved in tube formation,tube formation ability and expression level of VEGFA mRNA in the Huoxue Yiqi Prescription group were up-regulated,and the difference was statistically significant(P<0.05).Conclusion:MiR-484 inhibits the expression of VEGFA and the proliferation,migration and formation of HUVEC,and Huoxue Yiqi recipe has a certain negative regulation effect on this process,which may be a new target of Huoxue Yiqi recipe to promote angiogenesis.展开更多
[目的]探讨黄芩苷抑制人结肠黏膜上皮细胞凋亡的机制。[方法]黄芩苷处理人结肠黏膜上皮细胞,检测其凋亡水平与miRNA水平的变化。敲低筛选出的miRNA后,检测其凋亡水平与潜在靶标mRNA水平的变化。荧光素酶报告系统检测miRNA与潜在靶标的...[目的]探讨黄芩苷抑制人结肠黏膜上皮细胞凋亡的机制。[方法]黄芩苷处理人结肠黏膜上皮细胞,检测其凋亡水平与miRNA水平的变化。敲低筛选出的miRNA后,检测其凋亡水平与潜在靶标mRNA水平的变化。荧光素酶报告系统检测miRNA与潜在靶标的结合。[结果]黄芩苷处理人结肠黏膜上皮细胞后,人结肠黏膜上皮细胞的凋亡水平显著下降(0.35±0.11 vs 0.06±0.02,P<0.05)。黄芩苷处理人结肠黏膜上皮细胞后,miR-642a-3p、miR-484的表达水平显著下降(2.35±0.75 vs 0.51±0.18,1.94±0.63 vs 0.60±0.20,P<0.05)。敲低miR-484后,人结肠黏膜上皮细胞的凋亡水平显著降低(0.35±0.11 vs 0.07±0.02,P<0.05),PEA15和UAP1L1的水平显著升高(1.23±0.41 vs 2.45±0.64,0.65±0.23 vs 1.95±0.61,P<0.05)。敲低PEA15后,人结肠黏膜上皮细胞的凋亡水平显著升高(0.35±0.11 vs 0.67±0.020,P<0.05)。过表达PEA15后,人结肠黏膜上皮细胞的凋亡水平显著下降(0.35±0.10 vs 0.06±0.01,P<0.05)。miR-484靶向PEA15的3'端非编码区。[结论]黄芩苷处理人结肠黏膜上皮细胞后降低miR-484的表达、提高PEA15的表达,最终抑制了人结肠黏膜上皮细胞凋亡。展开更多
文摘目的 检测骨肉瘤血清miR-337-3p、miR-484、miR-582表达及其临床意义。方法 收集我院2016年1月至2022年1月期间收治的67例骨肉瘤患者的临床病历资料,另纳入同期60例健康志愿者作为对照组。采用DEGseq方法对血清样本进行全基因组miRNA分析,采用实时荧光定量PCR(qPCR)分析血清和组织样本中miRNA水平。受试者工作特征(ROC)曲线分析miRNA诊断骨肉瘤的效能。Logistic回归模型分析影响骨肉瘤新辅助化疗疗效的因素。Cox风险比例回归模型分析影响骨肉瘤预后的因素。结果 经全基因组miRNA分析,骨肉瘤患者血清中miR-337-3p、miR-484、miR-582、miR-1912、miR-4535的表达显著下调。qPCR检测结果显示,骨肉瘤血清miR-337-3p(0.519±0.249 vs. 1.015±0.420)、miR-484(0.669±0.254 vs. 1.029±0.515)、miR-582(0.516±0.258 vs. 0.996±0.381)均低于健康对照组(P<0.001),同时骨肉瘤组织中miR-337-3p、miR-484、miR-582相对表达量也低于癌旁正常组织(P<0.001)。骨肉瘤血清样本和组织样本中miR-337-3p、miR-484、miR-582表达量分别呈正相关(P<0.001)。ROC曲线显示血清miR-337-3p、miR-484、miR-582对于骨肉瘤有良好的诊断效能,联合曲线下面积(AUC)为0.960(95%CI:0.932~0.989)。Logistic回归分析和Cox风险比例回归模型分析均显示血清miR-337-3p、miR-484、miR-582表达水平是影响骨肉瘤新辅助化疗疗效和总生存的独立影响因素(P<0.05)。结论 血清miR-337-3p、miR-484、miR-582可作为骨肉瘤诊断、预测预后的生物标志物。
基金National Natural Science Foundation Project(No.81973780)。
文摘Objective:To explore the possible mechanism of Huoxue Yiqi recipe promoting angiogenesis by targeting VEGF with miR-484.Methods:The model of miR-484 overexpressed in HUVEC was prepared and intervened with Huoxue Yiqi Recipe Freeze-dried powder.The effects of Huoxue Yiqi Recipe on proliferation,migration and tubular forming ability of HUVEC were separatly detected by CCK-8 method,cell scratch test and Matrigel three-dimensional forming test in vitro.The effect of Huoxue Yiqi Recipe on VEGFA mRNA expression was detected by real-time fluorescence quantitative PCR.Results:Compared with the control group,the proliferation and migration area of cells in miR-484 overexpression model group decreased,the length,number of branches and nodes involved in tube formation decreased,the tube formation ability decreased significantly,and the expression level of VEGFA mRNA decreased,with statistical significance.Compared with the model group,the cell proliferation ability,migration area,length,number of branches and nodes involved in tube formation,tube formation ability and expression level of VEGFA mRNA in the Huoxue Yiqi Prescription group were up-regulated,and the difference was statistically significant(P<0.05).Conclusion:MiR-484 inhibits the expression of VEGFA and the proliferation,migration and formation of HUVEC,and Huoxue Yiqi recipe has a certain negative regulation effect on this process,which may be a new target of Huoxue Yiqi recipe to promote angiogenesis.
文摘[目的]探讨黄芩苷抑制人结肠黏膜上皮细胞凋亡的机制。[方法]黄芩苷处理人结肠黏膜上皮细胞,检测其凋亡水平与miRNA水平的变化。敲低筛选出的miRNA后,检测其凋亡水平与潜在靶标mRNA水平的变化。荧光素酶报告系统检测miRNA与潜在靶标的结合。[结果]黄芩苷处理人结肠黏膜上皮细胞后,人结肠黏膜上皮细胞的凋亡水平显著下降(0.35±0.11 vs 0.06±0.02,P<0.05)。黄芩苷处理人结肠黏膜上皮细胞后,miR-642a-3p、miR-484的表达水平显著下降(2.35±0.75 vs 0.51±0.18,1.94±0.63 vs 0.60±0.20,P<0.05)。敲低miR-484后,人结肠黏膜上皮细胞的凋亡水平显著降低(0.35±0.11 vs 0.07±0.02,P<0.05),PEA15和UAP1L1的水平显著升高(1.23±0.41 vs 2.45±0.64,0.65±0.23 vs 1.95±0.61,P<0.05)。敲低PEA15后,人结肠黏膜上皮细胞的凋亡水平显著升高(0.35±0.11 vs 0.67±0.020,P<0.05)。过表达PEA15后,人结肠黏膜上皮细胞的凋亡水平显著下降(0.35±0.10 vs 0.06±0.01,P<0.05)。miR-484靶向PEA15的3'端非编码区。[结论]黄芩苷处理人结肠黏膜上皮细胞后降低miR-484的表达、提高PEA15的表达,最终抑制了人结肠黏膜上皮细胞凋亡。
