期刊文献+
共找到235篇文章
< 1 2 12 >
每页显示 20 50 100
Sex differences in morphine-induced behavioral sensitization and social behaviors in ICR mice 被引量:4
1
作者 Bo ZHAN Hong-Yuan MA +1 位作者 Jian-Li WANG Chao-Bao LIU 《Zoological Research》 CAS CSCD 2015年第2期103-108,共6页
Gender and genetic strain are two prominent variants that influence drug abuse. Although certain sexrelated behavioral responses have been previously characterized in ICR mice, little is known about the effects of sex... Gender and genetic strain are two prominent variants that influence drug abuse. Although certain sexrelated behavioral responses have been previously characterized in ICR mice, little is known about the effects of sex on morphine-induced behavioral responses in this outbred strain. Therefore, in this study, we investigated the sex differences of morphine-induced locomotion, anxiety-like and social behaviors in ICR mice. After morphine or saline exposure for four consecutive days(twice daily), increased locomotion, more time spent in the central area, as well as attenuated rearing and self-grooming behaviors were found in morphine-treated females in an open field; no differences were found in locomotion and the time spent in the central area between male and female controls. When interacting with the samesex individuals, female controls were engaged in more social investigation, following, body contacting and self-grooming behaviors than controls; morphine exposure reduced contacting and self-grooming behaviors in females; in contrast, these effects were not found in males. These results indicate that female ICR mice are more prosocial and are more susceptible to morphine exposure than males. 展开更多
关键词 MORPHINE ICR mice Locomotion Social behavior
下载PDF
Di-(n-butyl)-phthalate-induced Oxidative Stress and Depression-like Behavior in Mice with or without Ovalbumin Immunization 被引量:5
2
作者 ZUO Hao Xiao LI Jin Quan +5 位作者 HAN Bing KE Chen Juan LIU Xu Dong ZHANG Yu Chao LI Li YANG Xu 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2014年第4期268-280,共13页
Objective To investigate the relationship between atopic allergy and depression and the role of DBP in the development of depression. Methods BALB/c mice were randomly divided into eight groups:saline;ovalbumin (OVA... Objective To investigate the relationship between atopic allergy and depression and the role of DBP in the development of depression. Methods BALB/c mice were randomly divided into eight groups:saline;ovalbumin (OVA)-immunized;saline+DBP (0.45 mg/kg·183;d); saline+DBP (45 mg/kg·d); DBP (0.45 mg/kg·d) OVA-immunized; DBP (45 mg/kg·d) OVA-immunized; saline+hydrocortisone (30 mg/kg·d); and hydrocortisone (30 mg/kg·d)-exposed OVA-immunized. Behavior (e.g. open-field, tail suspension, and forced swimming tests), viscera coefficients (brain and spleen), oxidative damage [e.g. reactive oxygen species (ROS), malondialdehyde (MDA), and glutathione (GSH)], as well as levels of IgE and IL-4, were then analyzed. Results In the saline and OVA groups, the degree of depression symptoms in mice increased with increasing DBP concentration. Additionally, the OVA-immunity groups were associated with more serious depressive behavior compared with the same exposure concentration in the saline group. Oxidative damage was associated with a dose-dependent increase in DBP in the different groups. IL-4 and IgE levels were associated with low-dose DBP stimulation, which changed to high-dose inhibition with increasing DBP exposure, possibly due to spleen injury seen at high DBP concentrations.Conclusion Development of an atopic allergy has the potential to increase the risk of depression in mice, and it seems that DBP helps OVA to exert its effect in our present model. Moreover, the results of our study implicate a certain connection between brain oxidative stress and depression, which deserves a further exploration. 展开更多
关键词 Di (n-butyl) phthalate Atopic allergy Depression mice Oxidative stress behavioral tests
下载PDF
Effects of Curcumin on Biological Behavior and NF-κB/TNF-α Pathway in Mice with Metastatic Bone Pain of Breast Cancer Induced by Walker 256 Cells 被引量:2
3
作者 Aijun Ji Mingchen Zhu 《Journal of Cancer Therapy》 2020年第6期339-350,共12页
<strong><em>Background. </em></strong>The active ingredient curcumin of traditional Chinese medicine was selected as the research object to investigate the possible mechanism of breast cancer m... <strong><em>Background. </em></strong>The active ingredient curcumin of traditional Chinese medicine was selected as the research object to investigate the possible mechanism of breast cancer metastatic bone pain in mouse walker 256 cells and the effect of curcumin on the NF-κB/TNF-α pathway in order to provide a new idea for clinical treatment of breast cancer metastatic bone pain. <strong><em>Methods.</em></strong> By establishing an animal model of breast cancer bone metastasis in walker 256 cells, the biological behavior of nude mice was observed on the 8th day after successful modeling. Meanwhile, the low dose group, middle dose group and high dose group of mice were given 15 mg/kg, 25 mg/kg, 50 mg/kg of curcumin solution intraperitoneally in 21 days, and the right cavity bone and spinal cord distended in mice (L4-L6) tissues were used to detect related factors, Immunohistochemical method was used to detect c-fos in spinal cord. Expression levels of RANK, NF-κB and TNF-α were detected by RT-PCR and Western blot. Meanwhile, serum levels of Cox2, il-6, leukotriene and PGE2 were detected.<strong><em> Results. </em></strong>Observing the biological behavior index of nude mice, we found that the mechanical pain and thermal pain threshold decreased (p < 0.05), and the cold pain and spontaneous pain scores increased significantly (p < 0.05). After group study, the expression of c-fos in the cancer pain model group was significantly higher than that in the normal control group (p < 0.05), and with the increase of curcumin dose, the expression of c-fos in the high dose group was significantly lower than that in the solvent model group (p < 0.05). The expression of RANK, NF-κB, TNF-α was higher than that of the normal control group and decreased gradually with the increase of curcumin dose, among which the expression of high dose group was significantly lower than that of solvent group (p < 0.05). RANK, NF-κB, TNF-α protein expression was higher than that of normal control group and gradually decreased with the increase of curcumin dose. The levels of Cox2, IL-6, leukotriene and PGE2 in serum decreased with the increase of curcumin dose, and the high dose group decreased significantly (p < 0.05). <em><strong>Conclusions. </strong></em>On the 8th day after the success of the animal model of breast cancer bone metastasis in Walker 256 cells, abnormal biological behaviors such as heat pain, cold pain sensation and spontaneous hyperalgesia were observed. Further studies have found that the increased expression of rank on osteoclasts induced up-regulated expression of NF-κB and c-fos, induced expression of TNF-α gene, and could induce synthesis and release of leukotriene, PGE2 through direct activation of cyclooxygenase, inflammatory media IL-6 cascade reaction, resulting in pathological pain and hypersensitivity. Traditional Chinese medicine active ingredient curcumin could reduce RANK expression of osteoclast, inhibit cell NF-κB and spinal cord c-fos activity, reduce TNF-α expression, inhibit Cox2 activity, and reduce the synthesis and release of inflammatory factors leukotriene and PGE2, thus exerting its analgesic effect, which provides new ideas and methods for clinical treatment of metastatic bone pain in breast cancer. 展开更多
关键词 CURCUMIN Breast Cancer Metastatic Bone Pain mice Biological behavior NF-B/TNF-α
下载PDF
Protective Effect of Curcumin on Anxiety, Learning Behavior, Neuromuscular Activities, Brain Neurotransmitters and Oxidative Stress Enzymes in Cadmium Intoxicated Mice 被引量:1
4
作者 Gasem M. Abu-Taweel Jamaan S. Ajarem Mohammad Ahmad 《Journal of Behavioral and Brain Science》 2013年第1期74-84,共11页
Cadmium (Cd) exposure can induce acute lethal health-related threats to humans since it has an exceptional ability to accumulate in living organisms and cause toxicological effects. Curcumin (Cur) on the other hand ha... Cadmium (Cd) exposure can induce acute lethal health-related threats to humans since it has an exceptional ability to accumulate in living organisms and cause toxicological effects. Curcumin (Cur) on the other hand has a wide variety of biological activities and several animal studies have suggested for a potential therapeutic or preventive effects against several ailments and infections. To study the effect of Cur on the toxicity of Cd, sixty Swiss-Webster strain male mice were divided into 6 groups of ten each at random. Group-1 served as the na?ve control and received no treatment. Group-2, 3 and 4 were the experimental controls and were administered once a day with a single oral dose of 50% dimethyl sulphoxide (DMSO), Cur (300 mg/kg) or Cd (100 mg/kg) respectively, for 2 weeks. Group-5 and 6 received Cur and Cd in combination once a day orally for 2 weeks except that Cur in a dose of 150 and 300 mg/kg to group 5 and 6 respectively, was administered one hour before Cd (100 mg/kg) administration to both groups. After treatment period, the animals were subjected to behavioral tests and thereafter, the animals were sacrificed for the estimation of neurotransmitters like serotonin (5-HT), dopamine (DA) and it’s metabolite 3,4-dihydroxyphenylacetic acid (DOPAC) as well as oxidative stress enzymes like lipid peroxides in the form of thiobarbituric acid–reactive substances (TBARS) and total glutathione (GSH) in the forebrain tissue. Cd reduced significantly the body weight gain, the locomotor activity, anxiety behavior in the plus maze and the learning capability (cognitive effect) in the shuttle-box test. Biochemical analysis further revealed that Cd exposure significantly altered the brain neurotransmitters and the oxidative stress enzymes. However, administration of Cur along with Cd had an ameliorating effect on all the behavioral and biochemical parameters studied herein and reduced the toxicity of Cd significantly and dose-dependently. Thus, Cur may be beneficial for anxiety, neuromuscular, and cognitive problems and protect from Cd intoxication. 展开更多
关键词 CURCUMIN CADMIUM Male mice ANXIETY Cognitive behaviors NEUROTRANSMITTERS Oxidative Stress
下载PDF
Effects of Bisphenol A on Testosterone Levels and Sexual Behaviors of Male Mice 被引量:1
5
作者 Zhijun Zang Suyun Ji +1 位作者 Tingting Xia Shizong Huang 《Advances in Sexual Medicine》 2016年第4期41-49,共10页
Objective: To evaluate the effects of bisphenol A (BPA) on testosterone levels and sexual behaviors of male mice. Methods: Forty 12-week-old male mice, 22 - 25 g, were randomly allocated into four equal groups (n = 10... Objective: To evaluate the effects of bisphenol A (BPA) on testosterone levels and sexual behaviors of male mice. Methods: Forty 12-week-old male mice, 22 - 25 g, were randomly allocated into four equal groups (n = 10 per group): the control group and three BPA exposure groups including low concentration group (10 mg/kg), middle concentration group (50 mg/kg) and high concentration group (100 mg/kg). Each mouse received BPA intraperitoneally injected for 21 consecutive days. Sexual behaviors and testosterone levels in serum and interstitial tissue of testis were measured after the last administration of BPA. Furthermore, we also observed the weights of sexual organs of each group, including testis, epididymis and seminal vesicle. Results: The mount latency in the high concentration group, the intromission latency in the middle and the high concentration group were 11.64 ± 2.67 min, 20.28 ± 3.40 min and 20.13 ± 2.06 min respectively. All of them were longer than the control group (all P < 0.05). The mount frequency, intromission frequency and copulatory efficacy in the high concentration group were 0.52 ± 0.15 numbers/min, 0.37 ± 0.12 numbers/min and 0.40 ± 0.03, which were all statistically lower than the control group (all P < 0.05). The average testis weights in the low, middle and high concentration group were 0.198 ± 0.032 g, 0.203 ± 0.037g and 0.183 ± 0.032 g, and the relative testis weight in the high concentration group was 0.637 ± 0.106. All of these data were lower than the control group (P < 0.05, P < 0.05, P < 0.01 and P < 0.05, respectively). The epididymis weight and the relative epididymis weight, the weight of seminal vesicle and the relative weight of seminal vesicle were 0.069 ± 0.010 g, 0.242 ± 0.040, 0.219 ± 0.042 g and 0.760 ± 0.143, and all of them were lower than the control group (all P < 0.05). The serum testosterone levels in the high concentration group, the intratesticular testosterone levels in the middle and the high concentration group were 7.88 ± 1.62 ng/ml, 75.5 ± 7.18 ng/g and 73.00 ± 9.57 ng/g, which were all lower than the control group (all P < 0.05). Conclusions: BPA can decline the testosterone levels in mice and inhibit their sexual behaviors. 展开更多
关键词 Bisphenol A TESTOSTERONE Sexual behavior mice
下载PDF
Long Term Low Salt Feeding Led to the Changes in Food Intake, Body Weight and Depressive-Like Behavior in Mice
6
作者 Cong Chen Jing Ge +1 位作者 Yan Sun Jiapei Dai 《Natural Science》 2022年第4期163-169,共7页
Low salt intake is associated with depression, but the experimental evidence is not clear. Sixty adult mice were randomly divided into four groups (mild, moderate and severe salt deficiency groups and control) during ... Low salt intake is associated with depression, but the experimental evidence is not clear. Sixty adult mice were randomly divided into four groups (mild, moderate and severe salt deficiency groups and control) during the seasonal splitting from winter to spring. The mice in the control group were fed with standard ordinary diet (salt content 0.26%), while in the mild, moderate and severe groups, the mice were fed with 10%, 30% and 50% salt deficient feedstuff, lasting for approximately 3 months. The results showed that the salt content of feed was negatively correlated with the food intake and body weight of mice. The sucrose preference test found that only the mild salt deficiency group had no differ-ence between the beginning and the end of the experiment, and the other three groups in-cluding the control, showed a significant decrease. These results suggest that dietary salt content has an impact on the food intake and body weight of mice and is associated with the emergence of depressive-like behavior. Furthermore, the seasonal splitting from winter to spring may also have a differential synergistic effect on the change of depression-like behavior associated with low salt intake in mice. 展开更多
关键词 Low Salt Feeding Food Intake Body Weight Depressive-Like behavior mice
下载PDF
Single Exposure to Antidepressants during Infancy Is Associated with Delayed Behavioral Changes in C57BL/6 Mice
7
作者 Kazuyuki Yamada 《World Journal of Neuroscience》 2016年第2期151-164,共14页
As serotoninergic transmission plays a crucial role in higher brain function in mammals, the disturbance of this system will likely have significant effects on emotion and cognition. Previous studies have reported tha... As serotoninergic transmission plays a crucial role in higher brain function in mammals, the disturbance of this system will likely have significant effects on emotion and cognition. Previous studies have reported that chronic treatment with Specific Serotonin Reuptake Inhibitors (SSRIs) during both late pregnancy and lactation was associated with abnormal behavior in adult rats. These data imply that disturbances in serotoninergic transmission during neurodevelopment may have negative effects on both the structure and function of the resultant adult brain. Therefore, the effect of a single exposure to an SSRI or a tricyclic antidepressant that preferentially inhibits serotonin reuptake during the pre-weaning period was examined in adult mice. An oral infusion of paroxetine (70 mg/kg), fluvoxamine (250 mg/kg), clomipramine (180 mg/kg), or saline was administered on postnatal day 14. Starting at 11 weeks of age, mice were assessed using a comprehensive behavioral test battery. Mice treated with paroxetine demonstrated altered behavior on the open field and hole-board tasks;those treated with fluvoxamine had behavioral changes on the light-dark box, hole-board, and sucrose preference tasks, while alteration in forced swimming and cued fear behavior were noted in mice treated with clomipramine. These results suggest that even a single administration of an antidepressant could have profound effects on behavior in adulthood, although the effects might differ dependent on the specific drug that was administered. 展开更多
关键词 Antidepressants Specific Serotonin Reuptake Inhibitors (SSRIs) Delayed Effect behavioral Test Battery mice
下载PDF
脓毒症对青年期雄性CD-1小鼠认知行为学的影响
8
作者 李宗寅 杨启纲 +3 位作者 张凯旋 刘高霞 王芳 陈贵海 《医学研究与战创伤救治》 CAS 北大核心 2024年第7期686-690,共5页
目的探究青年CD-1雄性小鼠经历盲肠结扎穿孔(CLP)术后发生认知障碍及其恢复情况。方法取7~8周龄CD-1雄性小鼠若干分别造模,设立CLP组和假手术(Sham)组。造模成功后,分别将存活的Sham和CLP小鼠喂养至术后第10、10、20、30和60天进行六臂... 目的探究青年CD-1雄性小鼠经历盲肠结扎穿孔(CLP)术后发生认知障碍及其恢复情况。方法取7~8周龄CD-1雄性小鼠若干分别造模,设立CLP组和假手术(Sham)组。造模成功后,分别将存活的Sham和CLP小鼠喂养至术后第10、10、20、30和60天进行六臂放射状水迷宫(RAWM)和物体位置再认测试。结果与Sham组相比,CLP小鼠术后30 d以内在RAWM中的学习期错误次数和潜伏期均高于Sham组(P<0.05),而在术后60 d差异无统计学意义(P>0.05);CLP小鼠术后60 d以内的RAWM记忆期错误次数和潜伏期都显著高于Sham组(P<0.05);在物体位置再认实验中,CLP小鼠术后10 d和20 d的10 min延迟、24 h延迟的新位置优先指数均低于Sham组(P<0.05),而术后30 d和60 d的10 min延迟、24 h延迟的新位置优先指数与Sham组比较差异均无统计学意义(P>0.05)。结论青年期CD-1雄性小鼠在CLP术后60 d时仍有显著性空间记忆损害,且RAWM是较敏感的评估方法。 展开更多
关键词 脓毒症 盲肠结扎穿孔 认知 行为 小鼠
下载PDF
基于小鼠急性醉酒评价的茶酒饮后行为研究
9
作者 冯华芳 吕杨俊 +7 位作者 郑涛 朱跃进 刘青青 蒋玉兰 潘俊娴 王霈菲 沈才洪 张士康 《酿酒科技》 2024年第11期23-26,共4页
为开展茶酒饮后行为的研究,挖掘茶酒的健康属性,本研究分别按0.15 mL/10 g剂量蒸馏水、40.8%vol造模用酒、40.8%vol茶酒基酒(不含茶叶提取物)、40.8%vol茶酒灌胃4组小鼠,并监测小鼠急性醉酒行为。结果表明,茶酒基酒组的醉酒率(67.67%)... 为开展茶酒饮后行为的研究,挖掘茶酒的健康属性,本研究分别按0.15 mL/10 g剂量蒸馏水、40.8%vol造模用酒、40.8%vol茶酒基酒(不含茶叶提取物)、40.8%vol茶酒灌胃4组小鼠,并监测小鼠急性醉酒行为。结果表明,茶酒基酒组的醉酒率(67.67%)低于造模用酒组(83.33%),饮后自主活动次数(99.20次)、转角转向次数(2.40次)显著高于造模用酒组;茶酒组的醉酒率(67.67%)低于造模用酒组,饮后自主活动次数(106.60次)、转角转向次数(4.80次)、前肢放置次数(1.50次)显著高于造模用酒组,同时高于基酒组,角落转向次数显著高于基酒组。综上,相对造模用酒,茶酒急性醉酒行为失调最轻微,基酒次之,表明茶酒及其基酒品质较好,饮后舒适度高。茶酒中茶叶提取物发挥显著的正向调节作用,是茶酒健康属性的物质基础之一。 展开更多
关键词 茶酒 茶叶提取物 急性醉酒小鼠 醉酒行为
下载PDF
健脾祛痰方对限制活动ApoE^(-/-)小鼠体成分/行为学变化的影响 被引量:1
10
作者 杜高乐 姜钧文 《中医临床研究》 2024年第4期16-20,25,共6页
目的:观察健脾祛痰方对限制活动载脂蛋白E基因敲除(Apolipoprotein E Knockout,ApoE^(-/-))小鼠体成分/行为学变化的影响。方法:将30只ApoE^(-/-)小鼠随机分为模型组,阿托伐他汀组以及健脾祛痰方低浓度组、健脾祛痰方中浓度组、健脾祛... 目的:观察健脾祛痰方对限制活动载脂蛋白E基因敲除(Apolipoprotein E Knockout,ApoE^(-/-))小鼠体成分/行为学变化的影响。方法:将30只ApoE^(-/-)小鼠随机分为模型组,阿托伐他汀组以及健脾祛痰方低浓度组、健脾祛痰方中浓度组、健脾祛痰方高浓度组;将6只C57BL/6J小鼠作为正常组。正常组以普通饲料喂养,其余5组以高脂饲料喂养,阿托伐他汀组进行阿托伐他汀2.4 mg/(kg·d)灌胃,健脾祛痰方低浓度组、健脾祛痰方中浓度组、健脾祛痰方高浓度组以健脾祛痰方[2.97 g/(kg·d)、5.94 g/(kg·d)、11.88 g/(kg·d)]灌胃,正常组和模型组以等体积生理盐水灌胃,12周后进行脾虚痰浊模型评价。实验期间每周观察小鼠的皮毛色泽、活动能力及大便性状,检测体成分,以旷场实验、跑台实验进行动物行为学评估。结果:与正常组比较,模型组小鼠旷场实验运动总距离减少,跑台实验跑步力竭时间降低,体脂降低,差异有统计学意义(P <0.01)。与模型组比较,健脾祛痰方低浓度组、健脾祛痰方中浓度组、健脾祛痰方高浓度组小鼠旷场实验运动总距离呈上升趋势,但差异无统计学意义(P> 0.05);健脾祛痰方中、高浓度组小鼠跑台实验跑步时间增加,差异有统计学意义(P <0.05,P <0.01);阿托伐他汀组和健脾祛痰方低浓度组跑步时间均呈上升趋势,但差异无统计学意义(P> 0.05);健脾祛痰方低浓度组、健脾祛痰方中浓度组、健脾祛痰方高浓度组小鼠体脂呈上升趋势,但差异无统计学意义(P> 0.05);阿托伐他汀组小鼠体脂显著增加,差异有统计学意义(P <0.01)。结论:健脾祛痰方可增加限制活动ApoE^(-/-)小鼠的行为活动,但对体成分改善作用不显著。 展开更多
关键词 健脾祛痰方 动脉粥样硬化 动物行为学 体脂 ApoE^(-/-)小鼠
下载PDF
基于红外孔板嗅觉测试仪在小鼠嗅觉功能研究中的应用
11
作者 刘彤彤 李婧 +4 位作者 董朝玉 王灿 解俊樊 侯一平 邵玉峰 《兰州大学学报(医学版)》 2024年第9期6-13,34,共9页
目的利用灵敏的红外传感器取代电容传感器并用于孔板嗅觉测试仪,建立小鼠嗅觉分辨、识别、空间记忆、偏好实验方法,以检验孔板嗅觉测试仪检测小鼠嗅觉功能的稳定性。方法分别采用习惯/去习惯行为测试、嗅觉空间记忆测试、丙酸嗅觉阈值... 目的利用灵敏的红外传感器取代电容传感器并用于孔板嗅觉测试仪,建立小鼠嗅觉分辨、识别、空间记忆、偏好实验方法,以检验孔板嗅觉测试仪检测小鼠嗅觉功能的稳定性。方法分别采用习惯/去习惯行为测试、嗅觉空间记忆测试、丙酸嗅觉阈值测试和嗅觉偏好检测正常C57 BL/6J小鼠的嗅觉功能。结果C57 BL/6J小鼠具有嗅觉辨别、嗅觉线索关联的空间记忆能力,对丙酸的嗅觉阈值为10^(-3)kPa,对香叶醇的偏好度最高,对左旋香芹酮的偏好度最低。结论本研究使用的孔板嗅觉测试仪能够满足小鼠嗅觉的辨别、识别、学习记忆以及偏好性等嗅觉行为学实验需求。 展开更多
关键词 嗅觉 嗅觉功能 嗅觉行为学 嗅觉测试仪 红外传感器 小鼠
下载PDF
Schisandra N-butanol extract improves synaptic morphology and plasticity in ovarectomized mice
12
作者 Meiyan Yang Zhaolin Cai +1 位作者 Peng Xiao Chuhua Li 《Neural Regeneration Research》 SCIE CAS CSCD 2012年第18期1365-1369,共5页
Preliminary work by our research team revealed that Schisandra, a renowned traditional Chinese medicine, causes learning and memory improvements in ovariectomized mice. This activity was attributed to active ingredien... Preliminary work by our research team revealed that Schisandra, a renowned traditional Chinese medicine, causes learning and memory improvements in ovariectomized mice. This activity was attributed to active ingredients extracted with N-butyl alcohol, named Schisandra N-butanol extract. In this study, ovariectomized mice were pretreated with Schisandra N-butanol extract given by intragastric administration. This treatment led to the enhancement of learning, and an increase in hippocampal CA1 synaptic, surface and postsynaptic density. A decrease in the average size of the synaptic active zone was also observed. These experimental findings showing that Schisandra N-butanol extract improved synaptic morphology indicate an underlying mechanism by which the ability of learning is enhanced in ovariectomized mice. 展开更多
关键词 Schisandra N-butanol extract OVARIECTOMY mice behavioral learning hippocampal CA1" synapticmorphology synaptic density neural regeneration
下载PDF
虚拟操作系统在小鼠视觉疾病动物模型行为评估中的价值
13
作者 焦洋(综述) 邵正波(审校) 《中华实验眼科杂志》 CAS CSCD 北大核心 2023年第8期822-826,共5页
视觉行为学检测是视觉疾病动物模型鉴定的主要方法之一,目前主要通过虚拟操作系统(VOS)产生视觉刺激诱发动物模型产生视动反应(OMR)或视动反射(OKR)来进行测量。自动化VOS能够调节光栅条纹宽度、旋转速度、光照强度等参数控制监测装置... 视觉行为学检测是视觉疾病动物模型鉴定的主要方法之一,目前主要通过虚拟操作系统(VOS)产生视觉刺激诱发动物模型产生视动反应(OMR)或视动反射(OKR)来进行测量。自动化VOS能够调节光栅条纹宽度、旋转速度、光照强度等参数控制监测装置的对比敏感度和空间频率阈值,并追踪OKR、OMR及OKR联合OMR运动。通过对巩膜搜索线圈法、角膜标记法、OMR-arena系统、OMR指数、阶梯测试协议等测量方法及评估指标的不断完善与优化,图形二维刺激升级为三维刺激,并引入计算机图像识别技术提取小鼠身体及头部轮廓,利用深度学习等计算机算法,分析并处理疾病小鼠视觉行为学数据,提高灵敏度,缩短测量时间,减少检测误差,增加数据精准度,从而获得更可靠的视功能评估结果,为青光眼、白内障、视网膜病变、遗传性眼病、视神经退行性病变等疾病研究提供有力的研究工具。本文主要从视觉检测方法和视力评估指标2个方面对现有自动化VOS在小鼠视觉疾病模型行为评估中的价值进行综述。 展开更多
关键词 动物模型 视觉 小鼠 虚拟操作系统 视功能 视觉行为学
下载PDF
槐定碱对骨癌痛小鼠的影响及其作用机制 被引量:1
14
作者 李俊达 宁星 +5 位作者 刘震 陈海韶 赵璐 李华道 欧华金 潘灵辉 《中国癌症防治杂志》 CAS 2023年第2期138-143,共6页
目的探讨槐定碱在骨癌痛小鼠中的影响及其作用机制。方法将24只C57BL/6小鼠随机分成假手术组(Sham组)、骨癌痛组(BCP组)、骨癌痛+生理盐水组(BCP+NS组)、骨癌痛+槐定碱组(BCP+Sophoridine组,25 mg/kg)。分别于癌细胞接种前1 d以及接种后... 目的探讨槐定碱在骨癌痛小鼠中的影响及其作用机制。方法将24只C57BL/6小鼠随机分成假手术组(Sham组)、骨癌痛组(BCP组)、骨癌痛+生理盐水组(BCP+NS组)、骨癌痛+槐定碱组(BCP+Sophoridine组,25 mg/kg)。分别于癌细胞接种前1 d以及接种后3 d、5 d、7 d、10 d和14 d时检测机械缩足阈值和热痛阈值;Western blot法检测脊髓L4-L5段背根神经节和大脑皮层组织中DAP12/Trem2/TLR4轴相关蛋白的表达水平。结果与Sham组相比,在术后5 d、7 d、10 d、14 d,BCP组机械缩足阈值及热痛阈值均明显降低(均P<0.05)。与BCP组相比,在术后5 d、7 d、10 d、14 d,BCP+Sophoridine组的热痛阈值均明显升高(均P<0.05);术后10 d、14 d,BCP+Sophoridine组机械缩足阈值均明显升高(均P<0.05)。Western blot实验结果显示,与BCP组相比,BCP+Sophoridine组小鼠背根神经节和大脑皮层组织中的TLR4蛋白表达水平均显著减少(均P<0.05),DAP12、Trem2蛋白表达水平均显著升高(均P<0.05)。结论槐定碱可减轻骨癌痛小鼠癌痛行为,其机制可能与调控DAP12/Trem2/TLR4轴有关。 展开更多
关键词 槐定碱 骨癌痛 DAP12/Trem2/TLR4轴 行为学 小鼠
下载PDF
大黄酚对CUMS抑郁小鼠行为学的改善作用 被引量:2
15
作者 蔡依琳 朱乐玫 +2 位作者 陈彬 李千喜 刘玉萍 《中国医药科学》 2023年第13期22-25,59,共5页
目的探讨大黄酚对慢性不可预知性应激刺激(CUMS)导致的小鼠的抑郁行为学的改善作用。方法将40只BALB/c小鼠分为对照组、模型组及大黄酚低(0.1 mg/kg)、中(1 mg/kg)、高剂量组(10 mg/kg),每组各8只。对模型组及大黄酚低、中、高剂量组给... 目的探讨大黄酚对慢性不可预知性应激刺激(CUMS)导致的小鼠的抑郁行为学的改善作用。方法将40只BALB/c小鼠分为对照组、模型组及大黄酚低(0.1 mg/kg)、中(1 mg/kg)、高剂量组(10 mg/kg),每组各8只。对模型组及大黄酚低、中、高剂量组给予CUMS建立小鼠抑郁模型,给药均采用腹腔注射方法。通过比较各组小鼠体重、糖水偏好度和抑郁样行为变化,测试大黄酚改善抑郁模型的效果。结果实验第21天,与对照组比较,模型组及大黄酚组体重和糖水偏好度显著降低(P<0.05),提示小鼠抑郁模型建立成功。实验第42天,与模型组相比,大黄酚低、中、高剂量组体重和糖水偏好度均显著上升(P<0.05),悬尾不动时间显著缩短(P<0.05),大黄酚中、高剂量组强迫游泳不动时间明显缩短(P<0.05)。结论大黄酚能有效改善CUMS大鼠的抑郁样行为。 展开更多
关键词 大黄酚 CUMS小鼠 抑郁模型 行为学
下载PDF
益母草碱调节Akt/MDM2/p53信号通路对脑胶质瘤细胞恶性生物学行为的影响 被引量:5
16
作者 黄超 方兴刚 +2 位作者 陈璐 陈汉玉 陈曾凤 《疑难病杂志》 CAS 2023年第10期1090-1097,共8页
目的观察益母草碱调节蛋白激酶B(Akt)/双微体同源基因2(MDM2)/p53信号通路对脑胶质瘤细胞恶性生物学行为的影响。方法2022年3—9月于湖北省十堰市太和医院实验室进行实验。采用CCK-8法测定不同浓度(0、0.4、0.8、1.2、1.6、2.0 mmol/L)... 目的观察益母草碱调节蛋白激酶B(Akt)/双微体同源基因2(MDM2)/p53信号通路对脑胶质瘤细胞恶性生物学行为的影响。方法2022年3—9月于湖北省十堰市太和医院实验室进行实验。采用CCK-8法测定不同浓度(0、0.4、0.8、1.2、1.6、2.0 mmol/L)益母草碱处理的小鼠脑胶质瘤细胞GL261存活率并筛选出其最佳作用浓度。体外培养GL261细胞并随机分为对照组、益母草碱组、益母草碱+SC79(Akt激活剂)组,以益母草碱1.6 mmol/L和5μmol/L的SC79分组处理后,采用蛋白免疫印记法检测各组细胞Akt/MDM2/p53通路相关蛋白表达;采用CCK-8法、流式细胞术、细胞划痕实验和Transwell实验分别检测各组细胞增殖、凋亡、迁移、侵袭情况。构建颅内胶质瘤小鼠模型30只并随机分为对照组、益母草碱低剂量组、益母草碱中剂量组、益母草碱高剂量组及益母草碱高剂量+SC79组,分组处理后以TUNEL染色检测各组小鼠颅内胶质瘤细胞凋亡情况;以免疫印记法检测各组胶质瘤组织Akt/MDM2/p53通路相关蛋白表达。结果(1)细胞实验:与对照组比较,益母草碱组细胞凋亡率、p53蛋白表达显著升高(P<0.05),存活率、迁移率、侵袭数、p-Akt/Akt与p-MDM2/MDM2降低(P<0.05);与益母草碱组比较,益母草碱+SC79组细胞凋亡率、p53蛋白表达降低(P<0.05),存活率、迁移率、侵袭数、p-Akt/Akt与p-MDM2/MDM2升高(P<0.05)。(2)动物实验:与对照组比较,益母草碱组胶质瘤组织细胞凋亡率、p53蛋白表达升高(t/P=20.076/<0.001、7.486/<0.001),p-Akt/Akt与p-MDM2/MDM2降低(t/P=11.769/<0.001、7.579/<0.001);与益母草碱组比较,益母草碱+SC79组胶质瘤组织细胞凋亡率、p53蛋白表达降低(t/P=18.328/<0.001、7.359/<0.001),p-Akt/Akt与p-MDM2/MDM2升高(t/P=9.640/<0.001、5.529/<0.001)。结论益母草碱可通过抑制Akt/MDM2/p53信号而抑制脑胶质瘤细胞增殖、迁移、侵袭及在小鼠体内生长,并诱导其凋亡,最终抑制其恶性进展。 展开更多
关键词 脑胶质瘤 益母草碱 Akt/MDM2/p53 恶性生物学行为 小鼠
下载PDF
长期自由选择饮酒小鼠模型的建立及其行为学评价 被引量:1
17
作者 袁婷婷 李燊 +1 位作者 吴燕 吴海涛 《北京大学学报(医学版)》 CAS CSCD 北大核心 2023年第2期315-323,共9页
目的:建立长期自由饮酒小鼠的模型模拟人类自愿长期饮酒的状态,探讨长期自由饮酒小鼠模型在运动、焦虑及认知行为方面的评价标准。方法:使用6周龄SPF级C57BL/6J雄性小鼠40只,随机分为长期自由饮酒组(n=20)和正常对照组(n=20)。两组小鼠... 目的:建立长期自由饮酒小鼠的模型模拟人类自愿长期饮酒的状态,探讨长期自由饮酒小鼠模型在运动、焦虑及认知行为方面的评价标准。方法:使用6周龄SPF级C57BL/6J雄性小鼠40只,随机分为长期自由饮酒组(n=20)和正常对照组(n=20)。两组小鼠固体饲料正常给予,长期自由饮酒组小鼠每日自由摄取10%(体积分数)酒精和水,正常对照组每日仅摄取水。饲养时间为7个月,并应用转棒实验、平衡木、矿场、高架十字、两箱社交行为学、新物体识别、Y迷宫、水迷宫等行为学测试对模型小鼠进行评价,每次进行行为学测试前需进行24 h的酒精戒断。结果:随着饮酒天数的增加,在酒精偏好性测试中小鼠表现出明显嗜酒现象,长期自由选择饮酒组小鼠随着酒精摄入的增加,皮毛略有光泽,饮食量减少,与对照组相比,从第3个月开始体质量增加有减缓的趋势,在第6个月和第7个月时体质量显著降低(P=0.006,P<0.001);在转棒和平衡木实验中小鼠表现出平衡运动能力减弱(P=0.003,P=0.001);在旷场和高架十字实验中小鼠有明显的焦虑样行为(P<0.001);在两箱社交行为学测试中小鼠社交能力减少(P<0.016);在新物体识别和Y迷宫实验测试中表现出对新物体的探索降低(P=0.018,P=0.040);在水迷宫中表现出学习和空间记忆等认知功能减弱(P<0.001)。结论:成功建立长期自由饮酒小鼠模型,为酒精成瘾的神经机制以及相关新靶点研究奠定了实验动物模型基础。 展开更多
关键词 长期 自由饮酒 小鼠模型 动物行为学
下载PDF
电针百会、神庭对脂多糖诱导的抑郁样小鼠行为及海马NOD样受体蛋白3炎症小体的影响 被引量:1
18
作者 杨柳笛 李红蕾 《河北中医》 2023年第10期1693-1697,1702,共6页
目的观察电针百会、神庭对脂多糖(LPS)诱导的抑郁样小鼠行为及NOD样受体蛋白3炎症小体(NLRP3)的影响。方法将32只雄性C57BL/6小鼠随机分为对照组、模型组、电针组和氟西汀组,每组8只。模型组、电针组、氟西汀组小鼠每日予腹腔注射脂多糖... 目的观察电针百会、神庭对脂多糖(LPS)诱导的抑郁样小鼠行为及NOD样受体蛋白3炎症小体(NLRP3)的影响。方法将32只雄性C57BL/6小鼠随机分为对照组、模型组、电针组和氟西汀组,每组8只。模型组、电针组、氟西汀组小鼠每日予腹腔注射脂多糖(LPS)建立急性抑郁小鼠模型,连续5天。在造模同时,电针组每日予百会、神庭穴电针治疗,氟西汀组每日予氟西汀20 mg/kg灌胃,对照组、模型组小鼠每日予等容积0.9%氯化钠注射液灌胃,连续5天。干预结束后采用旷场实验、强迫游泳实验进行行为学测试,评估各组小鼠行为学变化。采用蛋白免疫印迹法(Western Blot)检测海马组织NLRP3、半胱天冬酶-1(Caspase-1)、白细胞介素1β(IL-1β)的蛋白表达。结果旷场实验结果显示,各组小鼠的总运动总路程比较差异无统计学意义(P<0.05)。与对照组比较,模型组小鼠中央区域停留时间明显缩短(P<0.05);与模型组比较,电针组、氟西汀组小鼠中央区域停留时间均明显延长(P<0.05);与电针组比较,氟西汀组小鼠中央区域停留时间延长(P<0.05)。强迫游泳实验结果显示,与对照组比较,模型组小鼠不动时间明显延长(P<0.05);与模型组比较,电针组、氟西汀组小鼠不动时间均缩短(P<0.05);与电针组比较,氟西汀组小鼠不动时间缩短(P<0.05)。Western Blot结果显示,与对照组比较,模型组海马NLRP3、Caspase-1、IL-1β蛋白表达均升高(P<0.05);与模型组比较,电针组、氟西汀组NLRP3、Caspase-1、IL-1β蛋白表达均降低(P<0.05);电针组与氟西汀组组间比较差异无统计学意义(P>0.05)。结论电针治疗可显著改善LPS诱导的小鼠抑郁样行为,其作用机制可能与抑制NLRP3信号通路的活化有关。 展开更多
关键词 百会 神庭 抑郁症 小鼠 电针 行为 NOD样受体蛋白3炎症小体
下载PDF
奖励性操作条件反射实验在学习记忆研究中的应用 被引量:2
19
作者 张亦文 姚彩虹 +3 位作者 孙欣然 陈善广 刘新民 姜宁 《中国实验动物学报》 CAS CSCD 北大核心 2023年第6期802-810,共9页
动物行为学是用于评价动物学习记忆的基本实验手段,广泛应用于生理病理机制研究及药效评价。评价大小鼠学习记忆行为检测方法根据实验原理可分为惩罚性、自发性和奖励性三类,其中奖励性操作条件反射实验是基于奖励性原理和斯金纳条件反... 动物行为学是用于评价动物学习记忆的基本实验手段,广泛应用于生理病理机制研究及药效评价。评价大小鼠学习记忆行为检测方法根据实验原理可分为惩罚性、自发性和奖励性三类,其中奖励性操作条件反射实验是基于奖励性原理和斯金纳条件反射原理的行为学检测方法,为检测动物学习记忆中的高级认知功能提供了一种更全面更精细的检测手段,尤其适合于现代高科技条件下执行复杂操作任务时的认知作业能力评价。本文对奖励性操作性条件反射实验的发展历程、实验装置、实验方法及应用领域等方面进行了概述,为此类方法今后在国内学习记忆行为学领域的研究与应用提供参考。 展开更多
关键词 奖励性操作条件反射 学习记忆 行为学实验 大小鼠
下载PDF
基于APP/PS1双转基因小鼠与野生型小鼠比较综述学习记忆相关行为学实验优缺点和注意事项 被引量:4
20
作者 尚培骏 姬乃春 +3 位作者 杨锦雯 赵沅杰 拓振杰 张晓华 《中国实验动物学报》 CAS CSCD 北大核心 2023年第8期969-984,共16页
目的根据APP/PS1转基因AD模型小鼠和野生型小鼠在多种行为学实验中的表现,比较各行为学实验优缺点,并归纳其方法、注意事项、评价指标和应用特点,为研究动物认知相关行为学提供依据。方法采用Y迷宫、Ⅰ型T迷宫、Ⅱ型T迷宫、新物体识别... 目的根据APP/PS1转基因AD模型小鼠和野生型小鼠在多种行为学实验中的表现,比较各行为学实验优缺点,并归纳其方法、注意事项、评价指标和应用特点,为研究动物认知相关行为学提供依据。方法采用Y迷宫、Ⅰ型T迷宫、Ⅱ型T迷宫、新物体识别、旷场、转棒疲劳仪等实验,检测3月龄和6月龄APP/PS1转基因小鼠与野生型小鼠运动能力、探索行为、学习与记忆能力、新物体识别能力、活跃性、对旷场的畏惧程度以及中枢协调能力差别。结果APP/PS1转基因小鼠的工作记忆能力显著低于同月龄WT小鼠,表现为在Y迷宫内,6月龄的自主返回率显著增加,且6月龄AD小鼠的自主交替率和自主返回率较3月龄AD小鼠显著下降,WT小鼠没有看到这种变化。6月龄AD小鼠在I型T迷宫(奖赏型)中得分和记忆时间较WT小鼠显著下降,在Ⅱ型T迷宫(惩罚型)中学习时间显著增加,说明AD小鼠的学习能力弱于WT小鼠,且记忆提取较慢,正确率较低。在旷场实验中,6月龄AD小鼠运动距离和速度较同月龄野生型小鼠显著下降。AD小鼠进入中心区的次数和中心区/周围区时间比显著下降,直立次数也显著下降,说明了AD小鼠的探索行为较同月龄野生型小鼠显著下降,对旷场的畏惧程度更高。并未发现APP/PS1和WT小鼠在转棒疲劳仪掉落时间方面的差异,说明中枢协调能力无显著性差异。结论Y迷宫操作简单、受干扰小、实验周期短,但单只动物实验时间较长,适用于检测工作记忆;T迷宫符合小鼠的探索天性,可以最为准确测定小鼠学习与记忆能力,反映记忆的提取能力,但实验周期长;新物体识别实验利用小鼠对新物体的好奇心态,精准反映小鼠识物记忆的能力,但实验器材操作较为繁琐,且易受动物应激行为影响;旷场实验利用动物畏惧空旷场地的特性以及对新环境的好奇心,主要用来测定小鼠运动能力、探索能力、活跃性以及对旷场的畏惧程度,但实验结果易受小鼠紧张度影响;转棒疲劳实验直观地反映了小鼠的身体协调性和抗疲劳能力,间接反映小鼠的昼夜规律,适合作为检测学习记忆能力的辅助指标。 展开更多
关键词 APP/PS1转基因小鼠 阿尔茨海默病 动物行为学 学习记忆
下载PDF
上一页 1 2 12 下一页 到第
使用帮助 返回顶部