Active Micro-Nano-Collaborative Bioelectronic Device for Advanced Electrophysiological Recording

The development of precise and sensitive electrophysiological recording platforms holds the utmost importance for research in the fields of cardiology and neuroscience.In recent years,active micro/nano-bioelectronic devices have undergone significant advancements,thereby facilitating the study of electrophysiology.The distinctive configuration and exceptional functionality of these active micro-nano-collaborative bioelectronic devices offer the potential for the recording of high-fidelity action potential signals on a large scale.In this paper,we review three-dimensional active nano-transistors and planar active micro-transistors in terms of their applications in electroexcitable cells,focusing on the evaluation of the effects of active micro/nano-bioelectronic devices on electrophysiological signals.Looking forward to the possibilities,challenges,and wide prospects of active micro-nano-devices,we expect to advance their progress to satisfy the demands of theoretical investigations and medical implementations within the domains of cardiology and neuroscience research.

Synthesis, Structural Characterization and Antimicrobial Activity of a Novel Cobalt(II) Complex Based on 3-Methyl-1-Phenyl-4-(2-Thienoyl)-Pyrazol-5-One

New cobalt(II) complex, [Co(O2C15H11N2S)2(OH2)2]∙2H2O (1∙2H2O), has been synthesized upon reaction of cobalt chloride hexahydrate (Co(Cl)2∙6H2O) with 3-methyl-1-Phenyl-4-(2-thienoyl)-pyrazol-5-one (referred as HL) in ethanol at room temperature. Single crystal X-ray diffraction (XRD), spectroscopic methods, and microelemental analyses were used to characterize 1∙2H2O. Compound 1∙2H2O crystallizes in the orthorhombic crystal system with a Pbca space group and with the cobalt atom being pseudo-octahedral coordinated. The broth microdilution technique was used to screen the free ligand (HL) and the complex (1∙2H2O) for antimicrobial activities. HL has a low activity (MIC > 100 μg/mL) on all microorganisms, whereas compound 1∙2H2O displayed moderate activity (10 ∙2H2O exhibited bactericidal and fungicidal activity respectively on all the bacteria and yeasts tested. These findings reveal that the antimicrobial activity of HL was enhanced upon coordination to Co(II) ion against all microorganisms (bacteria and fungus).

Synthesis, Structural Characterization and Antimicrobial Activity of a Novel Cobalt(II) Complex Based on 3-Methyl-1-Phenyl-4-(2-Thienoyl)-Pyrazol-5-One

New cobalt(II) complex, [Co(O2C15H11N2S)2(OH2)2]∙2H2O (1∙2H2O), has been synthesized upon reaction of cobalt chloride hexahydrate (Co(Cl)2∙6H2O) with 3-methyl-1-Phenyl-4-(2-thienoyl)-pyrazol-5-one (referred as HL) in ethanol at room temperature. Single crystal X-ray diffraction (XRD), spectroscopic methods, and microelemental analyses were used to characterize 1∙2H2O. Compound 1∙2H2O crystallizes in the orthorhombic crystal system with a Pbca space group and with the cobalt atom being pseudo-octahedral coordinated. The broth microdilution technique was used to screen the free ligand (HL) and the complex (1∙2H2O) for antimicrobial activities. HL has a low activity (MIC > 100 μg/mL) on all microorganisms, whereas compound 1∙2H2O displayed moderate activity (10 ∙2H2O exhibited bactericidal and fungicidal activity respectively on all the bacteria and yeasts tested. These findings reveal that the antimicrobial activity of HL was enhanced upon coordination to Co(II) ion against all microorganisms (bacteria and fungus).

On the strengthening and slip activity of Mg-3Al-1Zn alloy with pre-induced{1012¯}twins

{1012¯}twins were introduced into the magnesium(Mg)plate AZ31 via pre-rolling along its transverse direction.The plates,both with and without the pre-induced{1012¯}twins,were subjected to uniaxial tension along different directions.Using crystal plasticity modeling,we found that the strengthening effect of the pre-induced{1012¯}twins on the macroscopic flow stress primarily arised from the increased slip resistance caused by the boundaries,rather than the orientation hardening due to the twinning reorientation(although the latter did make its contribution in some specific loading directions).Besides,the pre-existing{1012¯}twins were found,by both experiments and simulation,to promote the activity of prismatic and pyramidal<c+a>in the parent matrix of the material.Further analysis showed that the enhanced non-basal slip activity is related to the{1012¯}twin boundaries’low micro Hall-Petch slope ratios of non-basal slips to basal slip.With the critical resolved shear stress(CRSS)obtained from crystal plasticity modeling and the orientation data from EBSD,a probability-based slip transfer model was proposed.The model predicts higher slip transfer probabilities and thus lower strain concentration tendencies at{1012¯}twin boundaries than that at grain boundaries,which agrees with the experimental observation that the strain localization was primarily associated with the latter.The present findings are helpful scientifically,in deepening our understanding of how the pre-induced{1012¯}twins affect the strength and slip activity of Mg alloys,and technologically,in guiding the design of the pre-strain protocol of Mg alloys.

Synthesis and Antitumor Activity of 3-[2-(4-Hydroxy-Phenyl)-Ethyl]-Benzo[d] Isoxazole-4,6-Diol

A new phloretin derivative 1 3-[2-(4-hydroxy-phenyl)-ethyl]-benzo[d] isoxazole-4,6-diol (yield 63%) was synthesized from phloretin by carbonyl nucleophilic addition condensation reaction. Its structure was characterized by 1H NMR, 13C NMR and HR-MS. The phloretin, compound 1, resveratrol and acetylated resveratrol were determined by comparing them with paclitaxel. Anti-tumor activity of alcohol on SPC-A1, EC109, A549, MCF-7 and MDA-MB-231 cell lines. Compound 1 showed better antitumor activity than docetaxel against A549 tumor cells.

Effects of Microporous Structure of Activated Carbon on Adsorption Performance of N-butane

[Objective] The paper was to study the effect of microporous structure of ac- tivated carbon on adsorption performance of n-butane. [Method] Using 8 activated car- bons prepared from different materials and technologies, the effects of physical prop- erties of activated carbon on butane adsorption performance were investigated. [Result] Specific surface area, pore volume and pore size distribution of activated carbon exert- ed remarkable effects on butane adsorption. The activated carbon with high percent- age of micropore volume within the range of 1.2-2 nm possessed high butane activity. The level of butane retentivity rose with the increase of the volume of pore within the range of 0.5-0,9 nm, which led to smaller butan working capacity （BWC）. [Conclusion] The study provided reference for the adsorption research for activated carbon.

Expression, Purification and Insecticidal Activity Analysis of Cry1Ac without Helix α-1

[Objective] This study aimed to analyze the expression, purification and insecticidal activity of Cry1Ac without helix α-1 of domain I in E. coli. [Method] Plasmid of B. thuringiensis HD-1 was used as the template for amplification of Cry1Ac gene, which was linked with pET28a vector to construct the recombinant plasmid. After transformation and IPTG induction, expression of target protein was detected by using SDS-PAGE method. Target protein was extracted and coated on the surface of feed for H. armigera to determine the insecticidal activity. [Result] Cry1Acdela1 gene could normally express the target protein in E. coli. However, the target protein existed in the form of inclusion body. Results of bioassay analysis showed that under the same concentration, fatality rate of activated Cry1Ac toxin reached above 75%, while that of Cry1Acdelα1 was only about 10%. [Conclusion] E. coli-expressed Cry1Acdelα1 had no insecticidal activity against H. armigera.

基于微多普勒角点特征与Non-Local机制的穿墙雷达人体步态异常终止行为辨识技术

穿墙雷达能够穿透建筑物墙体,实现室内人体目标探测。利用深度学习提取不同肢节点的微多普勒特征,可以有效辨识障碍物后的人体行为。但是,当生成训练、验证集与生成测试集的受试者不同时,基于深度学习的行为识别方法测试准确率相对验证准确率往往较低,泛化能力较差。因此,该文提出一种基于微多普勒角点特征与Non-Local机制的穿墙雷达人体步态异常终止行为辨识技术。该方法利用Harris与Moravec检测器提取雷达图像上的角点特征,建立角点特征数据集;利用多链路并行卷积和Non-Local机制构建全局上下文信息提取网络,学习图像像素的全局分布特征;将全局上下文信息提取网络重复堆叠4次得到角点语义特征图,经多层感知机输出行为预测概率。仿真和实测结果表明,所提方法可以有效识别室内人体步行过程中存在的坐卧、跌倒等突发步态异常终止行为,在提升识别准确率、鲁棒性的前提下,有效控制泛化精度误差不超过6.4%。

A Pair of New Spirocyclic Alkaloid Enantiomers with TrxR Inhibitory Activities Were Isolated from Marine-Derived Aspergillus ruber TX-M4-1

One new spirocyclic alkaloid,5-isopentenyl-cryptoechinuline D(1),along with 11 known compounds(2–12),were iso-lated from a marine fungus Aspergillus ruber TX-M4-1.The structures of compounds 1–12 were elucidated by spectroscopic evi-dences.Compound 1 was initially isolated as an enantiomer,and further separation of 1 by chiral HPLC afforded a pair of enantio-mers,including(-)-5-isopentenyl-cryptoechinuline D(1a)and(+)-5-isopentenyl-cryptoechinuline D(1b).Their absolute configura-tions were elucidated by ECD spectroscopic data.Compounds 1a,5 and 10 could inhibit thioredoxin reductase(TrxR)activity with IC50 values of 6.2,36.3 and 18.6μmol L^(-1),respectively.Surface plasmon resonance(SPR)study also demonsrated the interactions between compounds 6,8 and Niemann-Pick C1 Like 1(NPC1L1)respectively,which indicate that compounds 6 and 8 are potential NPC1L1 inhibitors.

Inhibition of Micro RNA 219 Expression Protects Synaptic Plasticity via Activating NMDAR1, Ca MKIIγ,and p-CREB after Microwave Radiation

In recent decades,the potential health hazards of microwave exposure have been attracting increasing attention.Our previous studies have demonstrated that microwave exposure impaired learning and memory in experimental animal models[1,2].

Activity of the Fungicide JS399-19 Against Fusarium Head Blight of Wheat and the Risk of Resistance

This report reviews the characteristics of JS399-19, a novel cyanoacrylate fungicide. JS399-19 strongly inhibits the mycelial growth of the fungal plant pathogens of the genus Fusarium and exhibits great potential in controlling Fusarium head blight （FHB） on wheat and other cereals. The mode of action of JS399-19 is evidently different from that of benzimidazole （for example, carbendazim） and other sort of fungicides, making it a possible replacement for carbendazim in China to manage carbendazim-resistant subpopulations of Fusarium graminearum and F. asiaticum. JS399-t9 has excellent protective and curative activity against these pathogens. Incorrect use of this fungicide, however, is likely to select for resistance. Among JS399-19-resistant mutants of F. asiaticum induced in the laboratory, the resistant level of mutants was high and the phenotype of resistance against JS399-19 was conferred by a major gene by genetic analysis. The fitness of laboratory-induced JS399-19-resistant mutants of F. asiaticum was nearly equal to that of their parents. JS399-19 lacks cross resistance with other sort fungicides. To control FHB with JS399-19 and to delay the development of the fungicide-resistance, farmers should use tank mixtures containing JS399-19 and carbendazim, metconazole, tebuconazole, or prothioconazole.

Role of mitochondria in regulating micro RNA activity and its relevance to the central nervous system

Mitochondria serve as the powerhouse of cells,respond to cellular demands and stressors,and play an essential role in cell signaling,differentiation,and survival.Aberrant mitochondria function has been linked to diverse and complex human diseases such as neurodegenerative diseases,cancers,myopathies,premature aging,and metabolic syndromes（Nunnari and Suomalainen,2012）.

Calculation of Activity Coefficients for Components in Ti-15-3 Melt

Based on Kohler’s ternary solution model and Miedemaa’s model for calculating the heats of formation-△Hij of binary Systems, the integral equation has ben established for calculating the activity coefficients in ternarysolution. With the equation, the activity coefficients in Ti-15-3 melt have been calculated. The results show that allof components’ activity coefficients are less than 1, in other wolds, compared with ideal solution, all components takeon a negative deviation. At 2000K, the activity coefficients in Ti-15-3 melt are shown as: about 0.9 for Ti, about0.85 for V, about 0. 1 for Al, aboaut 0.5 for Cr and about 0.23 for Sn. me addition of V lowers the activity coefficient of Ti, but raise the ones of Al, Cr and Sn. These results can be directly used to calculate the evaporation loss ofAl, Cr and Sn during the ISM(Induction Skull Melting) process and at the same time estimate the reactivity betweenTi-15-3 melt and refractory during the cast production.

水淬锰渣-炉渣-石灰石复合微粉的活性试验

研究了经过物理激发的水淬锰渣微粉和炉渣微粉活性指数,进而确定水淬锰渣、炉渣适宜的比表面积;采用石膏、生石灰、水玻璃3种激发剂对水淬锰渣、炉渣、石灰石复合微粉进行了化学激发试验;探讨单一激发和复合激发对复合微粉的影响。结果表明:物理激发微粉时,水淬锰渣的适宜比表面积为470 m^(2)/kg,炉渣的适宜比表面积为410 m^(2)/kg;化学激发复合微粉时,使用4%的石膏和1%生石灰作为复合激发剂对复合微粉激发效果最佳,7、28 d活性指数达到最高,此时复合微粉7 d活性指数为55.7%,28 d活性指数为78.3%,均满足《用于水泥、砂浆和混凝土中的粒化高炉矿渣粉》(GB/T 18046—2017)中S75等级标准。

Characterization and Anti-tumor Activity of Glycopeptides from Ganoderma sinensis

The water-soluble part（GS） of Ganoderma sinense Zhao, Xu et Zhang was divided into high molecular（GS-H） and low molecular（GS-L） parts by Cellulose Super Filtration, and GS was also fractionated into four fractions, GS-1, 2, 3, and 4 by ethanol precipitation according to their molecular weights. Chemical analysis shows that GS and GS-1, 2, 3, 4 were complexes of polysaccharide and peptide. The fractions with molecular weights over 4000, GS-1, 2, 3, and GS-H show anti-tumor activities, however, the fractions with molecular weights lower than 4000, GS-4, and GS-L have no anti-tumor activity, indicating that the anti-tumor activity of Ganoderma Sinensis was caused by glucopeptides with molecular weight ranging from 4000 to 20000. Two purified glucopeptides, GS-6b and GS-7b were obtained from GS-H by ion-exchange and gel-permeation chromatography. Their molecular weights, glycosidic linkages, and configurations were detected by means of IR spectrum, sugar composition analysis, and methylation analysis. The polysaccharide parts of GS-6b and GS-7b had glucan backbone consisting of β-1→3 Glc, and side chain containing glucosyl, mannosyl, fucosyl, xylosyl, galactosyl, and glucuronic acid residues attached on 1-2, 1-4, 1-6 positions of the backbone of GS-6b, or 1-6, 1-4 positions of the backbone of GS-7b. The peptide parts in GS-6b and GS-7b were composed of 10 kinds of amino acids, including Asp, Ser, Arg, Gly, Thr, Pro, Ala, Val, Met, and Lys.

Synthesis, Crystal Structure and Antitumor Activity of (E)-4-tert-Butyl-N-(2,4-dichlorobenzylidene)-5-(1,2,4-triazol-1-yl)-thiazol-2-amine

The title compound （E）-4-tert-butyl-N-（2,4-dichlorobenzylidene）-5-（1,2,4-triazol-1-yl）-thiazol-2-amine was synthesized by the reaction of 4-tert-butyl-5-（1,2,4-triazol-1-yl）-thiazol-2-amine with 2,4-dichlorobenzaldehyde, and its crystal structure was determined by singlecrystal X-ray diffraction. The crystal belongs to the triclinic system, space group P1 with a = 7.9748（4）, b = 10.1803（5）, c = 11.4603（6）, α = 102.882（1）, β = 100.253（1）, γ = 104.457（1）°, V = 850.95（7）3, Z = 2, F（000） = 392, C16H15Cl2N5S, Mr = 380.29, Dc = 1.484 g/cm3, S = 1.095, μ = 0.512 mm-1, the final R = 0.0301 and wR = 0.0965 for 3334 observed reflections （I 〉 2σ（I））. The preliminary antitumor activity shows that for the title compound the IC50 of Hela is 0.175 μmol/mL and that of Bel7402 is 0.156 μmol/mL.

Synthesis, Crystal Structure and Fungicidal Activity of N-(4-tert-buty)-5-(1,2,4-triazol-1-yl)thiazol-2-yl)propionamide

The title compound has been synthesized by the reaction of 4-tert-butyl-5-（1,2,4- triazol-1-yl）-2-aminothiazole with propionic anhydride, and its crystal structure was determined by single-crystal X-ray diffraction. The crystal belongs to the orthorhombic system, space group Pbca with α = 18.441（2）, b = 8.3284（9）, c = 19.257（2） A, Z = 8, V = 2957.5（5） A3, Mr = 279.37, Dc = 1.255 mg/m3, S = 1.033, μ =0.219 mm^-1, F（000） = 1184, the final R = 0.0349 and wR = 0.0876 for 2629 observed reflections （I 〉 2σ（I））. X-ray crystal structure presents the intermolecular N–H···N hydrogen bond, which plays an important role in stabilizing the crystal structure. The preliminary bioassay indicates that the title compound exhibits potent fungicidal activity against R. Solani （25 mg/L） with inhibition rate of 80.0%.

Studies on Condensed Heterocyclic Compounds(Ⅲ)——Synthesis and Antibacterial Activity of 3-(4-Pyridyl)-6-Alkyl/Aromatic Heterocyclyl-s-Triazolo[3,4-b]-1, 3,4-Thiadiazoles and 1,4-Bis [6-(4-Pyridyl)-s-Triazolo [3,4-b]-1, 3,4-Thiadiazol-3-yl]-n-Butane

In recent years, fused heterocycles have been reported to possess important properties in synthesis and pharmacology. Especially, s-triazolo, 3,4-thiadiazole derivatives have been attracting much attention for chemists and pharmacologists because they show broad spectra of biological activities, such as antifungal, antibacterial, hy-

Active micro-vibration control based on improved variable step size LMS algorithm

The contradiction of variable step size least mean square(LMS)algorithm between fast convergence speed and small steady-state error has always existed.So,a new algorithm based on the combination of logarithmic and symbolic function and step size factor is proposed.It establishes a new updating method of step factor that is related to step factor and error signal.This work makes an analysis from 3 aspects:theoretical analysis,theoretical verification and specific experiments.The experimental results show that the proposed algorithm is superior to other variable step size algorithms in convergence speed and steady-state error.

miR-451a在肺结核患者血清中的表达水平及临床意义

目的 探讨活动性肺结核(ATB)患者血清微小RNA(miR)-451a、Toll样受体4(TLR4)表达水平与其预后的关系。方法 将350例肺结核患者根据诊断情况分为ATB组(162例)与非ATB组(NATB组,188例),另选180例健康体检者纳入对照组。ATB组依据预后分为预后良好组(120例)与预后不良组(42例)。检测血清miR-451a、TLR4 mRNA水平,分析ATB患者预后影响因素,Pearson法分析miR-451a与TLR4 mRNA相关性;绘制受试者工作特征(ROC)曲线分析血清miR-451a、TLR4 mRNA预测ATB患者预后的效能。结果 对照组、NATB组、ATB组血清miR-451a水平依次降低、TLR4 mRNA水平依次升高(均P<0.05)。ATB组血清miR-451a与TLR4 mRNA水平呈负相关(r=-0.519,P<0.05),血清miR-451a与C反应蛋白(CRP)、红细胞沉降率(ESR)、干扰素-γ(IFN-γ)、白细胞介素-12(IL-12)呈负相关(r=-0.404、-0.377、-0.435、-0.391,P<0.05),血清TLR4 mRNA与CRP、ESR、IFN-γ、IL-12呈正相关(r=0.469、0.361、0.442、0.384,P<0.05)。预后不良组血清miR-451a水平低于预后良好组,血清TLR4 mRNA水平高于预后良好组(P<0.05)。回归分析显示,TLR4 mRNA、miR-451a是ATB患者预后影响因素(P=0.001、0.009)。血清miR-451a联合TLR4 mRNA预测ATB患者预后不良的ROC曲线下面积(AUC)为0.949,优于单独检测miR-451a、TLR4 mRNA的0.852、0.821(P<0.05)。结论 ATB患者血清miR-451a水平降低、TLR4 mRNA水平升高,与炎症反应及预后密切相关,miR-451联合TLR4 mRNA对预后预测价值较高。