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Immune-related long noncoding RNA zinc finger protein 710-AS1-201 promotes the metastasis and invasion of gastric cancer cells
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作者 Wei Ding Wei-Wei Chen +4 位作者 Yi-Qin Wang Xue-Zhong Xu Yi-Bo Wang Yong-Min Yan Yu-Lin Tan 《World Journal of Gastrointestinal Oncology》 SCIE 2024年第2期458-474,共17页
BACKGROUND Gastric cancer(GC)is a prevalent malignant tumor of the gastrointestinal system.ZNF710 is a transcription factor(TF),and zinc finger protein 710(ZNF710)-AS1-201 is an immune-related long noncoding RNA(lncRN... BACKGROUND Gastric cancer(GC)is a prevalent malignant tumor of the gastrointestinal system.ZNF710 is a transcription factor(TF),and zinc finger protein 710(ZNF710)-AS1-201 is an immune-related long noncoding RNA(lncRNA)that is upregulated in GC cells.AIM To assess the correlation between ZNF710-AS1-201 and immune microenvir-onment features and to investigate the roles of ZNF710-AS1-201 in the invasion and metastasis processes of GC cells.METHODS We obtained data from The Cancer Genome Atlas and Wujin Hospital.We assessed cell growth,migration,invasion,and programmed cell death using cell counting kit-8,EdU,scratch,Transwell,and flow cytometry assays.Quantitative real-time polymerase chain reaction(qRT-PCR)was used to identify the potential downstream targets of ZNF710-AS1-201.RESULTS In GC tissues with low ZNF710-AS1-201 expression,immunoassays detected significant infiltration of various antitumor immune cells,such as memory CD8 T cells and activated CD4 T cells.In the low-expression group,the half-maximal inhibitory concentrations(IC_(50)s)of 5-fluorouracil,cisplatin,gemcitabine,and trametinib were lower,whereas the IC_(50)s of dasatinib and vorinostat were higher.The malignant degree of GC was higher and the stage was later in the high-expression group.Additionally,patients with high expression of ZNF710-AS1-201 had lower overall survival and disease-free survival rates.In vitro,the overexpression of ZNF710-AS1-201 greatly enhanced growth,metastasis,and infiltration while suppressing cell death in HGC-27 cells.In contrast,the reduced expression of ZNF710-AS1-201 greatly hindered cell growth,enhanced apoptosis,and suppressed the metastasis and invasion of MKN-45 cells.The expression changes in ZNF710 were significant,but the corresponding changes in isocitrate dehydrogenase-2,Semaphorin 4B,ARHGAP10,RGMB,hsa-miR-93-5p,and ZNF710-AS1-202 were not consistent or statistically significant after overexpression or knockdown of ZNF710-AS1-201,as determined by qRT-PCR.CONCLUSION Immune-related lncRNA ZNF710-AS1-201 facilitates the metastasis and invasion of GC cells.It appears that ZNF710-AS1-201 and ZNF710 have potential as effective targets for therapeutic intervention in GC.Nevertheless,it is still necessary to determine the specific targets of the ZNF710 TF. 展开更多
关键词 Gastric cancer ZNF710-AS1-201 Proliferation METASTASIS invasion Apoptosis
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Exploration of the regulatory effect of miR-21 on breast cancer cell line proliferation and invasion as well as the downstream target genes 被引量:9
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作者 Ying Kuang Ying-Jie Nie 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2016年第5期456-459,共4页
Objective: To study the regulatory effects of miR-21 on breast cancer cell line proliferation and invasion as well as the downstream target genes. Methods: Breast cancer cell lines MCF-7 were cultured and transfected ... Objective: To study the regulatory effects of miR-21 on breast cancer cell line proliferation and invasion as well as the downstream target genes. Methods: Breast cancer cell lines MCF-7 were cultured and transfected with miR-21 mimics and the corresponding negative control mimics(NC mimics), and then MTS kits were used to detect cell viability. Transwell experiment was used to detect cell invasion ability, and fluorescence quantitative PCR was used to detect the expression of proliferation and invasion-related genes in cells. Results: 24 h after transfection of miR-21 mimics and NC mimics, cell OD value and the number of invasive cells of miR-21 group were significantly higher than those of NC group, and m RNA contents of PDCD-4, Fas L, PTEN, Rho B, Maspin, TIMP3 and RECK in cells were significantly lower than those of NC group. Conclusion: miR-21 can promote the proliferation and invasion of breast cancer cell lines, and its downstream target genes include PDCD-4, Fas L, PTEN, Rho B, Maspin, TIMP3 and RECK. 展开更多
关键词 Breast cancer micro RNA PROLIFERATION invasion
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lncRNACNN3-206 activates intestinal epithelial cell apoptosis and invasion by sponging miR-212, an implication for Crohn’s disease 被引量:6
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作者 Na Li Rui-Hua Shi 《World Journal of Gastroenterology》 SCIE CAS 2020年第5期478-498,共21页
BACKGROUND Statistics indicate that the incidence of Crohn’s disease(CD)is rising in many countries.The poor understanding on the pathological mechanism has limited the development of effective therapy against this d... BACKGROUND Statistics indicate that the incidence of Crohn’s disease(CD)is rising in many countries.The poor understanding on the pathological mechanism has limited the development of effective therapy against this disease.Previous studies showed that long noncoding RNAs(lncRNAs)could be involved in autoimmune diseases including CD,but the detailed molecular mechanisms remain unclear.AIM To identify the differentially expressed lncRNAs in the intestinal mucosa associated with CD,and to characterize their pathogenic role(s)and related mechanisms.METHODS The differential expression of lncRNAs was screened by high-throughput RNA sequencing,and the top candidate genes were validated in an expanded cohort by real-time PCR.The regulatory network was predicted by bioinformatic software and competitive endogenous RNA analysis,and was characterized in Caco-2 and HT-29 cell culture using methods of cell transfection,real-time PCR,Western blotting analysis,flow cytometry,and cell migration and invasion assays.Finally,these findings were confirmed in vivo using a CD animal model.RESULTS The 3'end of lncRNACNN3-206 and the 3’UTR of Caspase10 contain highaffinity miR212 binding sites.lncRNACNN3-206 expression was found to be significantly increased in intestinal lesions of CD patients.Activation of the lncRNACNN3-206-miR-212-Caspase10 regulatory network led to increased apoptosis,migration and invasion in intestinal epithelial cells.Knockdown of lncRNACNN3-206 expression alleviated intestinal mucosal inflammation and tissue damage in the CD mouse model.CONCLUSION lncRNACNN3-206 may play a key role in CD pathogenesis.lncRNACNN3-206 could be a therapeutic target for CD treatment. 展开更多
关键词 Crohn’s disease microARRAY lncRNACNN3-206 Gene regulation Cell migration and invasion miR-212
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Inhibition of Invasion and Up-regulation of E-cadherin Expression in Human Malignant Melanoma Cell Line A375 by(-)-Epigallocatechin-3-gallate 被引量:3
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作者 吴艳 林云 +1 位作者 刘厚君 李家文 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2008年第3期356-359,共4页
The inhibitory effects of (-)-epigallocatechin-3-gallate (EGCG) on the invasion of human malignant melanoma cell line A375 and the possible molecular mechanisms of this effect were investiaged. A375 cells were pre... The inhibitory effects of (-)-epigallocatechin-3-gallate (EGCG) on the invasion of human malignant melanoma cell line A375 and the possible molecular mechanisms of this effect were investiaged. A375 cells were pretreated with 20 μg/mL EGCG for 24, 48 and 72 h respectively and the E-cadherin expression was detected by Western blot analysis. A375 cells were also pretreated with different concentrations of EGCG (1, 5, 10 and 20 μg/mL) for 72 h and the expression of E-cadherin was measured by RT-PCR. The adhesion and invasion of A375 cells were tested by cell-matrigel adhesion assay and matrigel invasion assay respectively. The results showed that EGCG could significantly up-regulate the expression of E-cadherin time-and concentration-dependently (both P〈0.05). Statistical analysis showed that A375 cells invasion was inhibited by EGCG and correlated with the up-regulation of E-cadherin expression. It was suggested that EGCG strongly inhibited invasion of A375 cells, and the inhibition mechanism was possibly associated with the up-regulation of E-cadherin expression. 展开更多
关键词 --epigallocatechin-3-gallate MELANOMA E-cadherin invasion
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Individual idea about the micro-invasive aspiration and drainage of intracranial hematoma 被引量:12
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作者 Zhouping Tang Feng Xu Xingyong Chen Xiangwu Meng Wei Hu Suiqiang Zhu Wei Wang 《Neural Regeneration Research》 SCIE CAS CSCD 2007年第12期751-759,共9页
AIM: This study aimed to expound the individual idea of micro-invasive surgery from pre-operative preparation, intra-operative processing and post-operative management. METHODS: Pre-operative preparation was improve... AIM: This study aimed to expound the individual idea of micro-invasive surgery from pre-operative preparation, intra-operative processing and post-operative management. METHODS: Pre-operative preparation was improved by analyzing pathological factors and hematoma property, and considering patients' age, basic disease, blood pressure control, with persistent haemorrhagia/rehaemorrhagia or not, operative occasion choice, positioning and other procedures. In the surgery, positioner was used. Initial aspiration volume was cautiously controlled. After operation, vital signs of patients were kept stable by cautiously using hematoma liquefacient and combining with free radical scavenger. RESULTS: The core content of individual micro-invasive surgery was mainly to relieve intracranial pressure. Under the condition of sufficient pre-operative preparation known by patients' family members, precise positioning was determined and individual therapeutic regimen was made. Meanwhile, caution should be taken in hematoma aspiration. Liquefaction and drainage should be paid more attention, and complications were processed actively. CONCLUSION: During the process of micro-invasive evacuation of intracranial hematoma for treating cerebral hemorrhage, attention should be paid to analyzing cerebral hematoma etiology and pathophysiological mechanism, and individual idea should be considered in surgical treatment aiming at patients' concrete disease condition. 展开更多
关键词 intracerebral hematoma INDIVIDUAL micro-invasive aspiration and drainage of intracranial hematoma
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Detection of D2-40 monoclonal antibody-labeled lymphatic vessel invasion in esophageal squamous cell carcinoma and its clinicopathologic significance 被引量:2
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作者 Bing Bai Wei Ma +7 位作者 Kai Wang Sita Ha Jian-Bo Wang Bing-Xu Tan Na-Na Wang Sheng-Si Yang Yi-Bin Jia Yu-Feng Cheng 《Cancer Biology & Medicine》 SCIE CAS CSCD 2013年第2期81-85,共5页
Objective: This study aims to investigate the clinicopathologic significance of lymphatic vessel invasion (LVI) labeled by D2-40 monoclonal antibody in esophageal squamous cell carcinoma (ESCC). Methods: Immunoh... Objective: This study aims to investigate the clinicopathologic significance of lymphatic vessel invasion (LVI) labeled by D2-40 monoclonal antibody in esophageal squamous cell carcinoma (ESCC). Methods: Immunohistochemical assay was used to detect the expression of D2-40 and LVI in 107 ESCC patients. Then, the correlation between the clinicopathologic feature and the overall survival time of the patients was analyzed. Results: The lymph node metastasis rates were 70% and 21% in the LVI-positive and LVI-negative groups, respectively. The nodal metastasis rate was higher in the LVI-positive group than in the LVI-negative group. Multivariate regression analysis showed that LVI was related to nodal metastasis (P〈0.001). The median survival time of the patients was 26 and 43 months in the LVI-positive and LVI-negative groups, respectively. Mthough univariate regression analysis showed significant difference between the two groups (P=0.014), multivariate regression analysis revealed that LVI was not an independent prognostic factor for overall survival in the ESCC patients (P=0.062). Lymphatic node metastasis (P=0.031), clinical stage (P=0.019), and residual tumor (P=0.026) were the independent prognostic factors. Conclusion: LVI labeled by D2-40 monoclonal antibody is a risk factor predictive of lymph node metastasis in ESCC patients. 展开更多
关键词 Esophageal squamous cell carcinoma lymphatic vessel invasion D2-40 lymph node metastasis PROGNOSIS
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Silencing Filamin A Inhibits the Invasion and Migration of Breast Cancer Cells by Up-regulating 14-3-3σ 被引量:1
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作者 Zhi-min JI Li-li YANG +5 位作者 Juan NI San-peng XU Cheng YANG Pei DUAN Li-ping LOU Qiu-rong RUAN 《Current Medical Science》 SCIE CAS 2018年第3期461-466,共6页
Filamin A and 14-3-3-σ are closely associated with the development of breast cancer. However, the exact relationship between them is still unknown. The present study aimed to examine the interaction of filamin A with... Filamin A and 14-3-3-σ are closely associated with the development of breast cancer. However, the exact relationship between them is still unknown. The present study aimed to examine the interaction of filamin A with 14-3-3-σ in the invasion and migration of breast cancer. RNA interference technology was employed to silence filamin A in MDA-MB-231 cells. Real-time PCR and Westem blotting were used to detect the expression of filamin A and 14-3-3-σ at mRNA and protein levels, respectively. Double immunofluorescence was applied to show their colocalization morphologically. Wound healing assay and Trans-well assay were used to testify the migration and invasion of MDA-MB-231 cells in filamin A-silenced cells. The results showed that silencing filamin A significantly increased the mRNA and protein levels of 14-3-3σ. In addition, double immunofluorescence displayed that filamin A and 14-3-3σ were predominantly colocalized in the cytoplasm of MDA-MB-231 cells. Silencing filamin A led to the enhanced fluorescence of 14-3-3σ. Furthermore, cell functional experiments showed that silencing filamin A inhibited the migration and invasion of MDA-MB-231 cells in vitro. In conclusion, silencing filamin A may inhibit the invasion and migration of breast cancer cells by upregulating 14-3-3σ. 展开更多
关键词 filamin A 14-3- SILENCE MAD-MB-231 cells invasion MIGRATION
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Treatment of Invasive Marine Species on Board by Using Micro-gap Discharge Plasma 被引量:6
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作者 张芝涛 白敏冬 +2 位作者 杨波 白敏菂 白希尧 《Plasma Science and Technology》 SCIE EI CAS CSCD 2005年第5期3025-3029,共5页
A pilot-scale experiment of 20t/h for the treatment of ship's ballast water is reported in this paper. When the concentration of the dissolved OH. was 0.68 mg/L during the experiment, the rate of destroying bacteria,... A pilot-scale experiment of 20t/h for the treatment of ship's ballast water is reported in this paper. When the concentration of the dissolved OH. was 0.68 mg/L during the experiment, the rate of destroying bacteria, mono-algae, protozoan reached 100% within 2.67 s. The effect of hydroxyl radicals on biochemical processes was also studied. The attenuation rate of photosynthesis pigment was 100%. And the main reason for the cells' death was the strong destruction of the monose, amylose, protein, DNA and RNA in the cells. As an advanced oxidation method, the procedure can destroy invasive marine species when a ship is in the process of discharging its ballast water. 展开更多
关键词 invasive marine species micro-gap discharge plasma photosynthesis pigment basic life substance
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Measurement of Ca^(2+) Flow in Cochlear Cells Using Non-Invasive Micro-Test Technique 被引量:1
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作者 CHEN Shi-qin YU Ning +2 位作者 YE Sheng-nan YANG Shi-ming ZHAI Suo-qiang 《Journal of Otology》 2010年第2期90-96,共7页
Objective To test Calcium ion(Ca2+) flow at the head and end of outer hair cells(OHCs) in resting state and in response to Nimodipine treatment.Methods Non-invasive micro-test techniques were used to study Ca2+ in iso... Objective To test Calcium ion(Ca2+) flow at the head and end of outer hair cells(OHCs) in resting state and in response to Nimodipine treatment.Methods Non-invasive micro-test techniques were used to study Ca2+ in isolated OHCs in adult guinea pigs.Results Four types of Ca2+ transport were identified in OHCs on basilar membrane tissue fragments:influx at the head of with efflux at the bottom(type 1):efflux at the head of OHCs with influx at the bottom(type 2);influx at the both head and bottom(type 3);and efflux at the both head and bottom(type 4).However,only type 1 and type 3 of Ca2+ ion transport were detected in the cochlea.We propose that Ca2+ ion transport exists in adult guinea pig cochlear OHCs in resting state and is variable.Ca2 + flow in OHC can be inhibited by Nimodipine in resting state. 展开更多
关键词 Guinea pig outer hair cells Ca2+ ion non-invasive micro-test technique nimodipine.
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基于micro-CT评估3种去龋方法清除龋损组织效果及微创潜力的研究
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作者 王茹燕 张力 刘苗苗 《口腔疾病防治》 2024年第9期695-701,共7页
目的使用微型计算机断层扫描(micro-computerized tomography,micro-CT)评价传统球钻去龋法、去腐凝胶辅助去龋法和龋显像笔辅助去龋法的清除龋损组织效果和微创潜力。方法本研究已获得医院生物医学研究伦理委员会审批及患者知情同意。... 目的使用微型计算机断层扫描(micro-computerized tomography,micro-CT)评价传统球钻去龋法、去腐凝胶辅助去龋法和龋显像笔辅助去龋法的清除龋损组织效果和微创潜力。方法本研究已获得医院生物医学研究伦理委员会审批及患者知情同意。收集30颗有牙本质龋的磨牙或前磨牙,随机分为3组,分别使用传统球钻去龋法(传统球钻组)、去腐凝胶去龋法(去腐凝胶组)和龋显像笔去龋法(龋显像笔组)对样本进行去龋处理,并记录每个样本的去龋操作时间,去龋前后均使用micro-CT进行扫描并记录每颗牙齿的龋损及健康牙体体积。根据去龋前后的龋损体积及去龋前后的健康牙体体积分别评估3种去龋方法的清除龋损组织效果、微创潜能。结果在去龋时间方面,去腐凝胶组所用时间(501.7±143.6)s高于传统球钻组(263.9±121.2)s和龋显像笔组(284.2±135.6)s,差异具有统计学意义(P<0.01);传统球钻组和龋显像笔组差异无统计学意义。在清除龋损组织效果方面,残余龋损体积与初始龋损体积的比值传统球钻组(0.087±0.04)最低,去腐凝胶组(0.51±0.10)最高,龋显像笔组(0.36±0.10)介于前两组之间,差异有统计学意义(P<0.01)。在微创潜能方面,去龋前后健康牙体体积比值传统球钻组(0.87±0.05)低于去腐凝胶组(0.99±0.01)和龋显像笔组(0.98±0.01),差异有统计学意义(P<0.01);去腐凝胶组和龋显像笔组差异无统计学意义。结论传统球钻组操作时间最短,但会过多去除健康牙本质和脱矿牙本质,微创潜能最差。去腐凝胶组可保留脱矿牙本质和全部健康牙本质,微创潜能最好,但清除龋损组织效果不佳,操作时间较长。龋显像笔组可以保留部分脱矿牙本质和健康牙本质,具有一定微创潜力,临床操作时间适中。 展开更多
关键词 龋齿 矿化牙本质 牙本质深龋 化学机械去龋 荧光辅助去龋 选择性去龋 龋齿微创治疗 微型计算机断层扫描 去龋效率
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Expression of mir-34c-5p and mir-150-5pin nasopharyngeal carcinoma and up-regulated expression after invasion and apoptosis of nasopharyngeal carcinoma cells
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作者 Xiaojun Zhou Baorui Lin Yun Wang 《Oncology and Translational Medicine》 CAS 2021年第1期25-30,共6页
Objective MiRNAs are closely related to tumors,and we hypothesized there is specific miR expression in nasopharyngeal carcinoma(NPC).We intended to investigate the expression of mir-34c-5p and mir-150-5p in NPC and to... Objective MiRNAs are closely related to tumors,and we hypothesized there is specific miR expression in nasopharyngeal carcinoma(NPC).We intended to investigate the expression of mir-34c-5p and mir-150-5p in NPC and to investigate the effects of mir-34c-5p and mir-150-5p on apoptosis and invasion following up-regulated expression in HNE1 NPC cells.Methods MiR-34c-5p and miR-150-5p expression levels in 30 individual cases of NPC and nasopharyngitis were detected with gene chip and qRT-PCR techniques.miR-34c-5p and miR-150-5p were transfected into the NPC cell line HNE1 via liposomes.Their expression levels were detected with qRT-PCR,apoptosis was evaluated by flow cytometry,and invasion ability was assessed via Transwell migration assay.Results MiR-150-5p expression levels in NPC and nasopharyngitis were 0.165±0.092 and 1.062±0.280 respectively,and miR-34c-5p expression levels in NPC and nasopharyngitis were 0.417±0.220 and 1.385±0.739,respectively,which indicated miR-34c-5p and miR-150-5p were weakly expressed in NPC.Apoptosis rates in HNE1 cells transfected by miR-34c-5p and miR-150-5p were increased,by 12.7%and 7.6%,respectively,which were significantly higher compared to blank control(3.9%).The Transwell assay demonstrated that invasive HNE1 cell counts were 32.00±2.00 and 28.33±2.08,respectively,compared to 60.66±8.50 in the blank control(P<0.001).Conclusion MiR-34c-5p and miR-150-5p are lowly expressed in NPC,and their down-regulation may be associated with NPC. 展开更多
关键词 miR-150-5p miR-34c-5p nasopharyngeal carcinoma APOPTOSIS invasion
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microRNA-373过表达可促进乳腺癌侵袭转移 被引量:10
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作者 丛竹军 张海俊 李漪 《中国免疫学杂志》 CAS CSCD 北大核心 2019年第10期1224-1226,共3页
目的:探讨microRNA-373在乳腺癌组织中表达与侵袭转移的相关性。方法:建立乳腺癌细胞MCF-7裸鼠移植瘤模型,采用qRT-PCR法检测癌组织和癌旁组织中miRNA-373的表达。分别转染空白试剂、miR-373阴性对照物、miR-373拟似物、miR-373阻遏物至... 目的:探讨microRNA-373在乳腺癌组织中表达与侵袭转移的相关性。方法:建立乳腺癌细胞MCF-7裸鼠移植瘤模型,采用qRT-PCR法检测癌组织和癌旁组织中miRNA-373的表达。分别转染空白试剂、miR-373阴性对照物、miR-373拟似物、miR-373阻遏物至MCF-7细胞,采用荧光实时定量PCR法检测细胞内miRNA-373含量,采用Transwell小室法检测MCF-7细胞侵袭能力。结果:qRT-PCR检测显示,乳腺癌组织miRNA-373表达显著低于癌旁组织,差异有统计学意义(P<0.05)。qRT-PCR检测显示,转染后BC组和NC组miRNA-373表达差异无统计学意义(P<0.05),MT组miRNA-373表达显著高于BC组、NC组和RT组(P<0.05),RT组miRNA-373表达显著低于BC组、NC组和MT组(P<0.05)。MT组穿膜细胞数显著低于BC组、NC组和RT组,RT组穿膜细胞数显著高于BC组、NC组和MT组,差异均有统计学意义(P<0.05);BC组和NC组穿膜细胞数相比较差异无统计学意义(P>0.05)。结论:乳腺癌MCF-7细胞的侵袭和迁移能力受miR-373调控,增强miRNA-373可降低MCF-7细胞侵袭和迁移能力,为乳腺癌治疗的新基因靶点药物的开发提供了思路。 展开更多
关键词 乳腺癌 micro-RNA 侵袭 表达
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上调microRNA-34a抑制胶质瘤细胞的增殖及侵袭 被引量:7
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作者 段军伟 唐晓平 +3 位作者 张涛 赵龙 彭华 段劼 《安徽医学》 2018年第1期6-9,共4页
目的探讨micro RNA-34a(mi R-34a)在胶质瘤组织中的表达情况以及对人脑胶质瘤细胞系U251增殖及侵袭的影响。方法选取川北医学院附属医院2013年3月至2017年3月胶质瘤组织标本30例和重症脑外伤需行手术者正常脑组织标本10例作为研究对象,... 目的探讨micro RNA-34a(mi R-34a)在胶质瘤组织中的表达情况以及对人脑胶质瘤细胞系U251增殖及侵袭的影响。方法选取川北医学院附属医院2013年3月至2017年3月胶质瘤组织标本30例和重症脑外伤需行手术者正常脑组织标本10例作为研究对象,采用荧光定量PCR法检测mi R-34a在胶质瘤组织表达情况。体外实验,将人工合成的mi R-34a模拟物mi R-34a mimic转染至U251细胞系,采用荧光定量PCR、MMT、Transwell法检测mi R-34a在U251细胞系中表达以及对U251细胞增殖和侵袭能力的影响。依据细胞转染情况,分为Control组(不转染任何基因)、Mock组(转染mi RNA-neg序列)和mi RNA-34a mimic组(转染mi RNA-34a mimic序列)。结果 mi RNA-34a在人脑胶质瘤组织中相对表达水平为(0.35±0.07),低于正常人脑组织的(1.00±0.13),差异有统计学意义(P<0.05);转染后,荧光定量PCR结果显示,Control组、Mock组、mi RNA-34a mimic组mi RNA-34a相对表达水平分别为(1.00±0.08)、(0.98±0.11)和(6.19±0.34),mi RNA-34a mimic组与Control、Mock组比较,差异有统计学意义(P<0.05);MMT结果显示,在不同时间点,mi RNA-34a mimic组U251细胞增殖抑制率高于Control组、Mock组,差异有统计学意义(P<0.05);Transwell结果显示,Control组、Mock组、mi RNA-34a mimic组侵袭细胞数分别为(90.53±5.84)个、(88.21±5.04)个和(27.46±2.76)个,mi RNA-34a mimic组与Control、Mock组比较,差异有统计学意义(P<0.05)。结论mi R-34a在胶质瘤组织中表达下调,上调mi R-34a表达可抑制胶质瘤细胞的增殖及侵袭。 展开更多
关键词 微小核糖核酸-34a 胶质瘤细胞 增殖 侵袭
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microRNA-155对于人膀胱癌细胞um-uc-3的迁移和侵袭能力影响的研究 被引量:2
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作者 彭杨 钟广正 +1 位作者 董文 林天歆 《岭南现代临床外科》 2015年第1期1-5,共5页
目的前期研究发现mi R-155在多种实体瘤中具有促肿瘤细胞增殖的作用,本研究探讨mi R-155对人膀胱癌细胞um-uc-3转移相关的生物学行为的影响。方法 (1)通过组织RNA研究,来讨论mi R-155在膀胱癌组织中的表达差异;(2)将人工合成mi R-155-mi... 目的前期研究发现mi R-155在多种实体瘤中具有促肿瘤细胞增殖的作用,本研究探讨mi R-155对人膀胱癌细胞um-uc-3转移相关的生物学行为的影响。方法 (1)通过组织RNA研究,来讨论mi R-155在膀胱癌组织中的表达差异;(2)将人工合成mi R-155-mimics转入人膀胱癌细胞um-uc-3,并设立对照组;(3)通过q RT-PCR检测mi R-155的转染效率;(4)划痕试验研究过表达mi R-155的um-uc-3细胞迁移能力变化;(5)Transwell试验检测mi R-155对um-uc-3细胞迁移、侵袭的影响;(6)Western-blot及q PCR检测肿瘤转移相关基因的表达情况。结果 (1)组织中,q RT-PCR证实mi R-155的表达量在膀胱癌组织中较癌旁组织增高;(2)q RT-PCR检测mi R-155转染效率,实验组表达量明显增高(P<0.01);(3)划痕实验证实mi R-155过表达的um-uc-3细胞有更强的迁移能力;(4)Transwell试验发现mi R-155可以促进um-uc-3细胞迁移、侵袭,与对照组差异显著(P<0.01);(5)过表达mi R-155的um-uc-3细胞中,β-catenin、vimentin、snail的表达量在RNA水平和蛋白水平都比对照组显著增高,但不涉及claudin-1。结论 mi R-155可以促进人膀胱癌细胞um-uc-3的迁移和侵袭。 展开更多
关键词 膀胱癌 微小RNA 迁移 侵袭 EMT
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5-羟色胺转运蛋白显像剂^(11)C-DASB的自动化合成及Micro PET/CT显像
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作者 张晓军 刘健 +2 位作者 李云钢 田嘉禾 张锦明 《同位素》 CAS 2015年第1期1-6,共6页
目的:自动化合成5-羟色胺转运蛋白显像剂11 C-DASB并进行大鼠Micro PET/CT显像;方法:通过改变甲基化试剂、溶解前体溶剂及反应条件,得到优化的标记条件作为碳-11多功能合成模块的输入参数,进行自动化合成11 C-DASB,大鼠静脉注射11 C-DAS... 目的:自动化合成5-羟色胺转运蛋白显像剂11 C-DASB并进行大鼠Micro PET/CT显像;方法:通过改变甲基化试剂、溶解前体溶剂及反应条件,得到优化的标记条件作为碳-11多功能合成模块的输入参数,进行自动化合成11 C-DASB,大鼠静脉注射11 C-DASB 45 min后进行显像;结果:采用11 C-CH3-Triflate作为甲基化试剂,通入新配制的含1mg去甲基DASB前体的500μL DMSO溶液内,80℃下加热2min,标准率为63.7%,大鼠显像表明,11 C-DASB特异性的浓聚于SERT富集区域;结论:经优化,11 C-DASB自动化合成可得到较高产率,大鼠显像表明,其特异性浓聚于SERT富集区域,有望作为5-羟色胺转运蛋白显像剂。 展开更多
关键词 ^11C-DASB 5-羟色胺转运蛋白 micro PET/CT
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microRNA-21逆向调控RECK促进乳腺癌细胞侵袭转移的研究 被引量:2
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作者 张佑民 《中国实用医药》 2016年第16期283-286,共4页
目的研究microRNA-21参与乳腺癌侵袭转移的调控机制。方法采用实时定量反转录-聚合酶反应(RT-PCR)方法检测MDA-435、MDA-231和MCF-7乳腺癌细胞株microRNA-21的表达;采用transwell方法测定上述三株乳腺癌细胞体外侵袭转移能力;人工合成mi... 目的研究microRNA-21参与乳腺癌侵袭转移的调控机制。方法采用实时定量反转录-聚合酶反应(RT-PCR)方法检测MDA-435、MDA-231和MCF-7乳腺癌细胞株microRNA-21的表达;采用transwell方法测定上述三株乳腺癌细胞体外侵袭转移能力;人工合成microRNA-21干扰序列,转染MDA-231、MDA-435乳腺癌细胞,观察对细胞侵袭能力的影响;应用Western blot检测逆转诱导蛋白(RECK)的表达;采用荧光报告基因实验检测RECK与microRNA-21的结合情况。结果 MCF-7、MDA-231及MDA-435细胞株中均具有microRNA-21高表达,且表达量MDA-435>MDA-231>MCF-7,其中,具有高侵袭特性的MDA-435细胞具有最高水平microRNA-21的表达为(4.51±0.71),MDA-231细胞具有中等水平的microRNA-21表达为(2.67±0.27),MCF-7细胞表达microRNA-21水平相对较低为(1.23±0.11),比较差异有统计学意义(P<0.01)。三株细胞中,MDA-435细胞侵袭力最高为(425.4±35.0)细胞数/视野,MCF-7最低为(142.7±13.4)细胞数/视野,MDA-231居中为(263.7±24.4)细胞数/视野,三组细胞侵袭力比较差异有统计学意义(P<0.01)。将anti-microRNA-21分别转染入MDA-231和MDA-435细胞,与对照组比较,两种细胞株转染anti-microRNA-21后分别引起56%和67%的microRNA-21表达量降低,比较差异有统计学意义(P<0.05)。将anti-microRNA-21转染入MDA-231细胞,与阴性对照组比较,anti-microRNA-21引起了34%细胞侵袭数目的减少。将anti-microRNA-21转染入MDA-435细胞,结果表明anti-microRNA-21引起68%侵袭细胞数目的下降。Western blot检测显示,microRNA-21表达下调,MDA-435细胞中RECK表达明显增强;荧光素酶实验显示共转染RECK和anti-microRNA-21的MDA-435细胞荧光酶活性增加38%。结论本研究提示microRNA-21可促进乳腺癌侵袭转移,其作用可能与逆向调控RECK有关。 展开更多
关键词 乳腺癌 侵袭 microRNA-21 伴Kazal域的富含半胱氨酸的逆转诱导蛋白 RNA干扰
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^(18)F-氟赤硝基咪唑micro PET/CT评价裸鼠乳腺癌早期放疗疗效实验研究 被引量:3
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作者 苏晓雨 徐慧琴 +3 位作者 汪会 余文静 张丹 谯凤 《安徽医科大学学报》 CAS 北大核心 2019年第2期203-206,共4页
目的探讨^(18)F-氟赤硝基咪唑micro PET/CT评价裸鼠MDA-MB231乳腺癌早期放疗疗效的价值。方法建立16只裸鼠MDA-MB231乳腺癌模型,将其按照随机对照原则分为两组:对照组(A组)、放疗组(B组),每组8只。每组裸鼠行micro PET/CT显像,测定每只... 目的探讨^(18)F-氟赤硝基咪唑micro PET/CT评价裸鼠MDA-MB231乳腺癌早期放疗疗效的价值。方法建立16只裸鼠MDA-MB231乳腺癌模型,将其按照随机对照原则分为两组:对照组(A组)、放疗组(B组),每组8只。每组裸鼠行micro PET/CT显像,测定每只裸鼠肿瘤SUVmax值。完成显像后,常规HE染色观察每组肿瘤组织形态学特征,免疫组化方法测定每组肿瘤细胞乏氧诱导因子-1α(HIF-1α)的表达情况。结果放疗前,对照组与放疗组SUVmax值差异无统计学意义(t=0. 375,P> 0. 05)。放疗组放疗后48 h裸鼠肿瘤组织SUVmax值较放疗前(t=9. 958,P <0. 05)、放疗后24 h(t=16. 506,P <0. 05)明显降低,差异有统计学意义(F=58. 860,P <0. 05)。放疗后24 h SUVmax值也低于放疗前24 h(t=5. 405,P <0. 05),差异有统计学意义。HE染色结果显示放疗组肿瘤细胞坏死较对照组更加明显。免疫组化结果显示放疗组放疗后HIF-1α表达阳性率明显低于放疗前(t=14. 802,P <0. 05),差异具有统计学意义。相关性分析结果显示肿瘤SUVmax与HIF-1α的表达呈明显正相关性(r=0. 865,P <0. 05)。结论^(18)F-氟赤硝基咪唑micro PET/CT可以监测肿瘤内部的乏氧状态,并且可以评价裸鼠MDA-MB231乳腺癌的早期放疗疗效。 展开更多
关键词 18F-氟赤硝基咪唑 micro PET/CT 放疗疗效 乏氧诱导因子-
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miR-216a和CAPN6基因过表达人宫颈癌细胞系HeLa增殖迁移侵袭变化及靶向关系
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作者 张贤雨 马欢 +5 位作者 宋凤丽 刘晓玉 李植燕 原娜 郝晓慧 张志林 《山东医药》 CAS 2024年第18期21-25,共5页
目的观察微小RNA-216a(miR-216a)和钙蛋白酶6(CAPN6)基因过表达的人宫颈癌细胞系HeLa增殖、迁移、侵袭变化及靶向关系,探讨miR-216a对HeLa细胞增殖迁移侵袭影响的作用机制。方法取对数生长期HeLa细胞,分为一、二、三、四、五组,一组转染... 目的观察微小RNA-216a(miR-216a)和钙蛋白酶6(CAPN6)基因过表达的人宫颈癌细胞系HeLa增殖、迁移、侵袭变化及靶向关系,探讨miR-216a对HeLa细胞增殖迁移侵袭影响的作用机制。方法取对数生长期HeLa细胞,分为一、二、三、四、五组,一组转染miR-216a mimic,二组转染pcDNA3.1-CAPN6质粒,三组顺序转染miR-216a mimic、pcDNA3.1-CAPN6,四组转染pcDNA3.1,五组转染miR-216a mimic NC,培养48 h时分别采用CCK-8法、划痕愈合实验、Transwell试验观察各组细胞的增殖迁移侵袭情况,培养24 h时采用qRT-PCR法检测各组细胞miR-216a、采用WesternBlotting法检测各组细胞CAPN6及信号转导子与激活子3(STAT3)蛋白。取对数生长期HeLa细胞分为四组:A组细胞顺序转染miR-216a mimic、pGL3-CAPN6-WT质粒,B组细胞顺序转染miR-216a mimic、pGL3-CAPN6-MUT质粒,C组细胞顺序转染miR-216amimicNC、pGL3-CAPN6-WT,D组细胞顺序转染miR-216amimicNC、pGL3-CAPN6-MUT,培养36 h时收集各组细胞,采用双荧光素酶报告基因检测试剂盒测算各组细胞相对荧光素酶活性。结果与五组相比,培养48 h时一组细胞增殖活性及划痕前缘迁移距离百分比低、侵袭细胞数少(P均<0.01);与四组相比,二组细胞增殖活性及划痕前缘迁移距离百分比高、侵袭细胞数多(P均<0.01);与一组相比,三组细胞增殖活性及划痕前缘迁移距离百分比高、侵袭细胞数多(P均<0.01);与二组相比,三组细胞增殖活性及划痕前缘迁移距离百分比低、侵袭细胞数少(P均<0.01)。与五组相比,一组细胞miR-216a相对表达量高(P<0.01)。与四组相比,培养24 h时二组细胞CAPN6蛋白、p-STAT3/STAT3相对表达量高,三组细胞表达CAPN6蛋白、p-STAT3/STAT3相对表达量低;与五组相比,一组细胞CAPN6蛋白、p-STAT3/STAT3相对表达量低;与一组相比,三组细胞CAPN6蛋白、p-STAT3/STAT3相对表达量高。与B组相比,A组细胞荧光素酶活性低(P<0.05)。结论miR-216a过表达可抑制HeLa细胞的增殖侵袭和迁移。HeLa细胞中miR-216a与CAPN6基因存在靶向关系。miR-216a可能通过调控CAPN6基因表达,抑制HeLa的增殖、迁移及侵袭。 展开更多
关键词 微小RNA 微小RNA-216a 钙蛋白酶6 细胞增殖 细胞侵袭 细胞迁移 宫颈癌
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MicroRNA-129-2靶向调控SOX4抑制食管癌细胞增殖与侵袭转移的研究 被引量:2
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作者 肖晶鑑 岳海英 +3 位作者 韦婷婷 周平婷 康敏 王仁生 《安徽医学》 2015年第12期1442-1446,共5页
目的探讨Micro RNA-129-2(mi R-129-2)在体内外抑制食管癌细胞增殖和侵袭转移的作用及可能机制,为食管癌的预后评估及靶向治疗提供新的思路。方法将mi R-129-2 mimics及mi R-129-2 mimics NC基因序列分别转染到食管癌NMC109细胞中,并设... 目的探讨Micro RNA-129-2(mi R-129-2)在体内外抑制食管癌细胞增殖和侵袭转移的作用及可能机制,为食管癌的预后评估及靶向治疗提供新的思路。方法将mi R-129-2 mimics及mi R-129-2 mimics NC基因序列分别转染到食管癌NMC109细胞中,并设为mi R-129-2 mimics高表达组和mi R-129-2 mimics阴性对照组(mi R-129-2 mimics NC组),将非转染的NMC109细胞设为空白对照组。体外实验:运用MTT法检测3组细胞的增殖能力、Transwell法检测细胞侵袭能力、蛋白印迹法检测SOX4蛋白表达。采用裸鼠体内移植瘤形成实验检测3组细胞在体内环境下的增殖能力。结果 mi R-129-2 mimics组的食管癌细胞增殖活性及侵袭能力较mi R-129-2 mimics NC组及空白对照组明显减弱(P<0.001),而空白对照组与mi R-129-2 mimics NC组食管癌细胞增殖性及侵袭性无明显差异(P>0.05);mi R-129-2 mimics组的食管癌细胞中,SOX4的表达下调(P<0.001);mi R-129-2 mimics组裸鼠移植瘤体积及重量明显减小(P<0.001),而mi R-129-2mimics NC组及空白对照组无明显差异(P>0.05)。结论 mi R-129-2具有抑制食管癌细胞NMC109增殖和侵袭转移的作用,其作用机制可能与靶向下调SOX4基因表达有关。 展开更多
关键词 miR-129-2 SOX4 食管癌 增殖 侵袭
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microRNA-29c对人胰腺癌细胞侵袭转移的抑制作用及机制 被引量:3
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作者 周显飞 田舍 +3 位作者 王杰 贾亮亮 喻超 江建新 《贵阳医学院学报》 CAS 2016年第9期997-1001,共5页
目的:探讨microRNA-29c对人胰腺癌细胞(As PC-1、Bx PC-3、PANC-1、MIA Pa Ca-2)生物学特性的影响。方法:培养1种人正常胰腺上皮细胞(HPDE)及4种人胰腺癌细胞(As PC-1、Bx PC-3、PANC-1、MIA Pa Ca-2),采用real-time PCR法观察5种细胞系... 目的:探讨microRNA-29c对人胰腺癌细胞(As PC-1、Bx PC-3、PANC-1、MIA Pa Ca-2)生物学特性的影响。方法:培养1种人正常胰腺上皮细胞(HPDE)及4种人胰腺癌细胞(As PC-1、Bx PC-3、PANC-1、MIA Pa Ca-2),采用real-time PCR法观察5种细胞系中microRNA-29c的表达差异,以microRNA-29c过表达腺病毒感染的PANC-1和MIA Pa Ca2细胞作为实验组,以空载体感染的PANC-1和MIA Pa Ca2细胞作为阴性对照组,采用细胞划痕实验、Transwell法检测两组细胞体外侵袭能力,Western blot检测两组细胞上皮间充质转化(EMT)相关蛋白波形蛋白(Vimentin)及E-钙粘蛋白(E-cadherin)的表达。结果:real-time-PCR显示各胰腺癌细胞系中microRNA-29c水平明显低于正常胰腺细胞系(P<0.05),细胞划痕实验发现感染腺病毒48 h后实验组PANC-1、MIA Pa Ca-2细胞的迁移距离明显短于阴性对照组(P<0.05),Transwell小室细胞侵袭实验发现实验组PANC-1和MIA Pa Ca-2细胞侵袭数量明显低于阴性对照组(P<0.05);Western blot蛋白免疫印迹结果显示PANC-1和MIA Pa Ca-2细胞过表达microRNA-29c后,Vimentin表达减少,E-cadherin表达增加。结论:microRNA-29c的过表达可有效抑制胰腺癌细胞的体外侵袭与转移,可能与Vimentin表达减少,E-cadherin表达增加有关,有望成为胰腺癌生物治疗的潜在靶点。 展开更多
关键词 微小RNA microRNA-29c 胰腺癌 腺病毒 细胞侵袭 转移 细胞划痕实验
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