Micro-motion effect in inverse synthetic aperture radar imaging of ballistic mid-course targets

For ballistic mid-course targets,in addition to constant orbital motion,the target or any structure on the target undergoes micro-motion dynamics,such as spin,precession and tumbling.The micro-motion characteristics of the ballistic mid-course targets were discussed.The target motion model and inverse synthetic aperture radar(ISAR) imaging model for this kind of targets were built.Then,the influence of micro-motion on ISAR imaging based on the established imaging model was presented.The computer simulation to get mid-course target echoes from static darkroom electromagnetic scattering data based on the established target motion model was realized.The imaging results of computer simulation show the validity of ISAR imaging analysis for micro-motion targets.

Micro-motion dynamics analysis of ballistic targets based on infrared detection

The dynamic characteristics related to micro-motions, such as mechanical vibration or rotation, play an essential role in classifying and recognizing ballistic targets in the midcourse, and recent researches explore ways of extracting the micro-motion features from radar signals of ballistic targets. In this paper, we focus on how to investigate the micro-motion dynamic characteristics of the ballistic targets from the signals based on infrared (IR) detection, which is mainly achieved by analyzing the periodic fluctuation characteristics of the target IR irradiance intensity signatures. Simulation experiments demonstrate that the periodic characteristics of IR signatures can be used to distinguish different micro motion types and estimate related parameters. Consequently, this is possible to determine the micro-motion dynamics of ballistic targets based on IR detection.

Analytical models for the penetration of semi-infinite targets by rigid,deformable and erosive long rods

A theoretical study is presented herein on the pen- etration of a semi-infinite target by a spherical-headed long rod for Yp 〉 S, where Yp is the penetrator strength and S is the static target resistance. For Yp 〉 S, depending upon initial impact velocity, there exist three types of penetration, namely, penetration by a rigid long rod, penetration by a deforming non-erosive long rod and penetration by an erosive long rod. If the impact velocity of the penetrator is higher than the hydrodynamic velocity （VH）, it will penetrate the target in an erosive mode; if the impact velocity lies between the hydrodynamic velocity （VH） and the rigid body velocity （VR）, it will penetrate the target in a deformable mode; if the impact velocity is less than the rigid body velocity （VR）, it will penetrate the target in a rigid mode. The critical conditions for the transition among these three penetration modes are proposed. It is demonstrated that the present model predictions correlate well with the experimental observations in terms of depth of penetration （DOP） and the critical transition conditions.

MiR-183-5p promotes the progression of non-small cell lung cancer through targeted regulation of FOXO1

Objective To investigate miR-183-5p targeting to forkhead box protein O1(FOXO1)and its corresponding effect on the proliferation,migration,invasion,and epithelial-mesenchymal transition(EMT)of non-small cell lung cancer(NSCLC)cells.Methods NSCLC tissues and adjacent normal tissues from 60 patients with NSCLC adenocarcinoma were obtained via pathological biopsy or intraoperative resection.Several cell lines were cultured in vitro,including the human normal lung epithelial cell line BEAS-2B and human NSCLC cell lines A549,SPCA-1,PC-9,and 95-D.miR-183-5p and FOXO1 mRNA expression in tissues and cells were detected by qRT-PCR;the corresponding correlations in NSCLC tissues were analyzed using the Pearson test,and the relationship between miR-183-5p expression and clinicopathological parameters was analyzed.The miR-183-5p-mediated regulation of FOXO1 was verified by bioinformatics prediction alongside double luciferase,RNA-binding protein immunoprecipitation(RIP)assay,and pull-down experiments.A549 cells were divided into control,anti-miR-NC,anti-miR-183-5p,miR-NC,miR-183-5p,miR-183-5p+pcDNA3.1,and miR-183-5p+pcDNA3.1-FOXO1 groups.Cell proliferation,invasion,migration,apoptosis,and cell cycle distribution were detected using an MTT assay,clone formation assay,Transwell assay,scratch test,and flow cytometry,respectively.The expression of EMT-related proteins in the cells was analyzed by western blotting.The effect of miR-185-3p silencing on the development of transplanted tumors was detected by analyzing tumor formation in nude mice.Results miR-183-5p expression was significantly higher in NSCLC tissues and cells than in adjacent normal tissues,whereas FOXO1 mRNA expression was significantly down-regulated.There was a significant negative correlation between miR-183-5p and FOXO1 mRNA in NSCLC tissues(P<0.05).Additionally,the expression of miR-183-5p was significantly correlated with tumor size,tumor differentiation,and tumor-node-metastasis stage in patients with NSCLC(P<0.05).miR-183-5p targeted and inhibited FOXO1 expression.Compared to the anti-miR-NC group,the cell proliferation,scratch healing rate,N-cadherin and vimentin protein expression,and the proportion of S phase cells were significantly lower in the anti-miR-183-5p group,whereas the protein expression of E-cadherin andα-catenin and the proportion of G0/G1 phase cells were significantly higher;additionally,the frequency of colony formation and invasion were significantly lower in the anti-miR-183-5p group(P<0.05).Compared to the miR-NC group,the cell proliferation,scratch healing rate,N-cadherin and vimentin protein expression,and the proportion of S phase cells in the miR-183-5p group were significantly higher,whereas the E-cadherin andα-catenin protein expression and the proportion of G0/G1 phase cells were significantly lower;furthermore,the frequency of colony formation and invasion were significantly higher in the miR-183-5p group(P<0.05).Compared with the miR-183-5p+pcDNA3.1 group,the OD value,scratch healing rate,N-cadherin and vimentin protein expression,and the proportion of S phase cells were significantly lower in the miR-183-5p+pcDNA3.1-FOXO1 group,whereas E-cadherin andα-catenin protein expression and the proportion of G0/G1 phase cells were significantly higher;additionally,the frequency of colony formation and invasion was significantly lower in the miR-183-5p+pcDNA3.1-FOXO1 group(P<0.05).Overall,silencing miR-185-3p inhibited the growth of transplanted tumors and promoted FOXO1 expression.Conclusion Overexpression of miR-183-5p can inhibit apoptosis and promote the proliferation,migration,invasion,and EMT,of NSCLC cells by down-regulating FOXO1 expression.

MicroRNAs as disease progression biomarkers and therapeutic targets in experimental autoimmune encephalomyelitis model of multiple sclerosis

Multiple sclerosis is an autoimmune neurodegenerative disease of the central nervous system characterized by pronounced inflammatory infiltrates entering the brain,spinal cord and optic nerve leading to demyelination.Focal demyelination is associated with relapsing-remitting multiple sclerosis,while progressive forms of the disease show axonal degeneration and neuronal loss.The tests currently used in the clinical diagnosis and management of multiple sclerosis have limitations due to specificity and sensitivity.MicroRNAs(miRNAs)are dysregulated in many diseases and disorders including demyelinating and neuroinflammatory diseases.A review of recent studies with the experimental autoimmune encephalomyelitis animal model(mostly female mice 6–12 weeks of age)has confirmed miRNAs as biomarkers of experimental autoimmune encephalomyelitis disease and importantly at the pre-onset(asymptomatic)stage when assessed in blood plasma and urine exosomes,and spinal cord tissue.The expression of certain miRNAs was also dysregulated at the onset and peak of disease in blood plasma and urine exosomes,brain and spinal cord tissue,and at the post-peak(chronic)stage of experimental autoimmune encephalomyelitis disease in spinal cord tissue.Therapies using miRNA mimics or inhibitors were found to delay the induction and alleviate the severity of experimental autoimmune encephalomyelitis disease.Interestingly,experimental autoimmune encephalomyelitis disease severity was reduced by overexpression of miR-146a,miR-23b,miR-497,miR-26a,and miR-20b,or by suppression of miR-182,miR-181c,miR-223,miR-155,and miR-873.Further studies are warranted on determining more fully miRNA profiles in blood plasma and urine exosomes of experimental autoimmune encephalomyelitis animals since they could serve as biomarkers of asymptomatic multiple sclerosis and disease course.Additionally,studies should be performed with male mice of a similar age,and with aged male and female mice.

微小RNA-34a-5p通过靶向鼠双微体4抑制巨噬细胞氧化应激损伤的作用

目的探讨微小RNA-34a-5p(miR-34a-5p)通过靶向鼠双微体4(MDM4)抑制巨噬细胞氧化应激损伤的作用。方法THP-1人单核细胞复苏后,培养分化为巨噬细胞。设立空白对照组。剩余细胞加入人源氧化低密度脂蛋白,孵育48 h,分化为巨噬泡沫细胞。设立动脉粥样硬化(AS)对照组。miR-34a-5p质粒转染,设为miR-34a-5p质粒转染组。每组24个重复。比较三组巨噬细胞凋亡情况,丙二醛(MDA)、超氧化物歧化酶(SOD)蛋白及活性氧(ROS)水平,MDM4阳性率。结果空白对照组凋亡率为(2.55±0.32)%,AS对照组凋亡率为(28.16±4.23)%,miR-34a-5p转染组为(13.48±1.66)%,三组比较差异有统计学意义(P<0.05)。AS对照组高于空白对照组与miR-34a-5p转染组,miR-34a-5p转染组高于空白对照组,差异有统计学意义(P<0.05)。三组MDA、SOD蛋白及ROS水平比较,差异均有统计学意义(P<0.05);AS对照组MDA、ROS水平最高,SOD水平最低,其次为miR-34a-5p转染组,组间比较差异均有统计学意义(P<0.05)。空白对照组MDM4阳性率为(8.33±0.35)%,AS对照组为(29.26±3.48)%,miR-34a-5p转染组为(14.26±1.59)%,三组比较差异有统计学意义(P<0.05)。结论miR-34a-5p可下调MDM4,进而抑制巨噬细胞氧化应激损伤。

5-羟色胺转运蛋白显像剂^(11)C-DASB的自动化合成及Micro PET/CT显像

目的:自动化合成5-羟色胺转运蛋白显像剂11 C-DASB并进行大鼠Micro PET/CT显像;方法:通过改变甲基化试剂、溶解前体溶剂及反应条件,得到优化的标记条件作为碳-11多功能合成模块的输入参数,进行自动化合成11 C-DASB,大鼠静脉注射11 C-DASB 45 min后进行显像;结果:采用11 C-CH3-Triflate作为甲基化试剂,通入新配制的含1mg去甲基DASB前体的500μL DMSO溶液内,80℃下加热2min,标准率为63.7%,大鼠显像表明,11 C-DASB特异性的浓聚于SERT富集区域;结论:经优化,11 C-DASB自动化合成可得到较高产率,大鼠显像表明,其特异性浓聚于SERT富集区域,有望作为5-羟色胺转运蛋白显像剂。

^(18)F-氟赤硝基咪唑micro PET/CT评价裸鼠乳腺癌早期放疗疗效实验研究

目的探讨^(18)F-氟赤硝基咪唑micro PET/CT评价裸鼠MDA-MB231乳腺癌早期放疗疗效的价值。方法建立16只裸鼠MDA-MB231乳腺癌模型,将其按照随机对照原则分为两组:对照组(A组)、放疗组(B组),每组8只。每组裸鼠行micro PET/CT显像,测定每只裸鼠肿瘤SUVmax值。完成显像后,常规HE染色观察每组肿瘤组织形态学特征,免疫组化方法测定每组肿瘤细胞乏氧诱导因子-1α(HIF-1α)的表达情况。结果放疗前,对照组与放疗组SUVmax值差异无统计学意义(t=0. 375,P> 0. 05)。放疗组放疗后48 h裸鼠肿瘤组织SUVmax值较放疗前(t=9. 958,P <0. 05)、放疗后24 h(t=16. 506,P <0. 05)明显降低,差异有统计学意义(F=58. 860,P <0. 05)。放疗后24 h SUVmax值也低于放疗前24 h(t=5. 405,P <0. 05),差异有统计学意义。HE染色结果显示放疗组肿瘤细胞坏死较对照组更加明显。免疫组化结果显示放疗组放疗后HIF-1α表达阳性率明显低于放疗前(t=14. 802,P <0. 05),差异具有统计学意义。相关性分析结果显示肿瘤SUVmax与HIF-1α的表达呈明显正相关性(r=0. 865,P <0. 05)。结论^(18)F-氟赤硝基咪唑micro PET/CT可以监测肿瘤内部的乏氧状态,并且可以评价裸鼠MDA-MB231乳腺癌的早期放疗疗效。

基层精准扶贫下“微腐败”的清廉建设路径与方法探析--以党政问责的演进过程为视角

在精准扶贫深入开展的过程中,“微腐败”问题层出不穷。对于“微腐败”官员的问责问题,我国各地的党政问责法律制度不断完善,使党政问责机制的整体发展呈渐进模式。但基于精准扶贫下“微腐败”的隐蔽性和发现的不及时性,加之我国传统问责文化环境的影响,有效的党政问责往往难以实现。本文从党政问责的演进过程入手,在精准扶贫视角下,通过回顾“微腐败”的生成机理及特点,总结了我国在探寻“微腐败”党政问责方面存在的几个问题,并在此基础上提出了相应的完善措施,为进一步治理“微腐败”提供了途径本文主要围绕以下五个问题展开:我国传统党政问责模式的缺陷分析问责对象与主体的局限性分析;问责方面的滞后性和不到位性分析;带病提拔与复出问题的规制;单一问责方式的缺陷。

MicroRNA-regulated viral vectors for gene therapy

Safe and effective gene therapy approaches require targeted tissue-specific transfer of a therapeutic transgene.Besides traditional approaches, such as transcriptional and transductional targeting, micro RNA-dependent posttranscriptional suppression of transgene expression has been emerging as powerful new technology to increase the specificity of vector-mediated transgene expression. Micro RNAs are small non-coding RNAs and often expressed in a tissue-, lineage-, activation- or differentiation-specific pattern. They typically regulate gene expression by binding to imperfectly complementary sequences in the 3' untranslated region(UTR) of the m RNA. To control exogenous transgene expression, tandem repeats of artificial micro RNA target sites are usually incorporated into the 3' UTR of the transgene expression cassette, leading to subsequent degradation of transgene m RNA in cel s expressing the corresponding micro RNA. This targeting strategy, first shown for lentiviral vectors in antigen presenting cells, has now been used for tissue-specific expression of vector-encoded therapeutic transgenes, to reduce immune response against the transgene, to control virus tropism for oncolytic virotherapy, to increase safety of live attenuated virus vaccines and to identify and select cell subsets for pluripotent stem cell therapies, respectively. This review provides an introduction into the technical mechanism underlying micro RNA-regulation, highlights new developments in this field and gives an overview of applications of micro RNA-regulated viral vectors for cardiac, suicide gene cancer and hematopoietic stem cell therapy, as well as for treatment of neurological and eye diseases.

MicroRNA-mediated interactions between host andhepatitis C virus

Micro RNAs(mi RNAs) are small noncoding RNAs. More than 2500 mature mi RNAs are detected in plants, animals and several types of viruses. Hepatitis C virus(HCV), which is a positive-sense, singlestranded RNA virus, does not encode viral mi RNA. However, HCV infection alters the expression of host mi RNAs, either in cell culture or in patients with liver disease progression, such as liver fibrosis, cirrhosis, and hepatocellular carcinoma. In turn, host mi RNAs regulate HCV life cycle through directly binding to HCV RNAs or indirectly targeting cellular m RNAs. Increasing evidence demonstrates that mi RNAs are one of the centered factors in the interaction network between virus and host. The competitive viral and host RNA hypothesis proposes a latent cross-regulation pattern between host m RNAs and HCV RNAs. High loads of HCV RNA sequester and de-repress host mi RNAs from their normal host targets and thus disturb host gene expression, indicating a means of adaptation for HCV to establish a persistent infection. Some special mi RNAs are closely correlated with liver-specific disease progression and the changed levels of mi RNAs are even higher sensitivity and specificity than those of traditional proteins. Therefore, some of them can serve as novel diagnostic/prognostic biomarkers in HCVinfected patients with liver diseases. They are also attractive therapeutic targets for development of new anti-HCV agents.

Synthesis Hexadecyl 3-{4-[2-Hydroxy-3(isopropylamino)propoxy] phenyl}propionate as Potential Ligand for Liposome Targeting to Ischemic Myocardium

Novel hexadecyl 3- { 4-[2-hydroxy-3（isopropylamino）propoxy]phenyl }propionate （HPP）was synthesized and its effect on delivery of liposomes into cultured cardiomyocytes was examined. The structure of HPP was characterized by IH NMR, 1R and MS. The amount of cardiomyocytes uptake of HPP-liposome was 3.9-fold higher than plain-liposome, and the increase was 6.2-fold when hypoxia happens. It indicated that HPP was a potential ligand for liposome targeting to ischemic myocardium.

Effect of Surface Roughness in Micro-nano Scale on Slotted Waveguide Arrays in Ku-band

Modeling of the roughness in micro-nano scale and its influence have not been fully investigated, however the roughness will cause amplitude and phase errors of the radiating slot, and decrease the precision and efficiency of the SWA in Ku-band. Firstly, the roughness is simulated using the electromechanical coupled（EC） model. The relationship between roughness and the antenna＇s radiation properties is obtained. For verification, an antenna proto- type is manufactured and tested, and the simulation method is introduced. According to the prototype, a contrasting experiment dealing with the flatness of the radiating plane is conducted to test the simulation method. The advantage of the EC model is validated by comparisons of the EC model and two classical roughness models （sine wave and fractal function）, which shows that the EC model gives a more accurate description model for roughness, the maxi- mum error is 13%. The existence of roughness strongly broadens the beamwidth and raises the side-lobe level of SWA, which is 1.2 times greater than the ideal antenna. In addition, effect of the EC model＇s evaluation indices is investigated, the most affected scale of the roughness is found, which is 1/10 of the working wavelength. The proposed research provides the instruction for antenna designing and manufacturing.

Evaluating the evolving evidence: The challenges of molecular-targeted therapy in management of gastric cancer

Over the past decade, a multitude of molecular targeted agents have been explored in the treatment of advanced metastatic gastric. Recent advances in molecular signaling pathways that are dysregulated in gastric cancer lead to the development of new targeted therapies for the treatment of advanced and metastatic gastric cancer. The addition of trastuzumab to first-line chemotherapy is now a standard of care for the treatment of Human epidermal growth factor receptor (HER2-) positive advanced or metastatic disease, and other HER2-targeted therapies are in late-stage clinical development. Findings from recent major clinical trials provide important insight into the future of metastatic gastric cancer management, which may include the use of anti-angiogenesis, Mesenchymal epithelial transition factor (MET) and Hedgehog Pathways Inhibitortherapy across multiple treatment lines, in the salvage setting, and as part of novel regimens in combination with other targeted agents.

Micro-Doppler effect testing technique for attitude of projectile in space flight

To measure projectile attitude in space flight, based on continuous wave （CW） radar, a new micro-Doppler effect testing technique is developed in this paper. It also establishes radar testing model for attitude of flying projectile and resolve micro-Doppler effect of projectile motion attitude. By distinguishing and geting attitude parameters such as micro-motion period, this technique can in- tuitively estimate the flight stability of projectile, and the validity of this technique is proved accord- ing to flight tests.

Potential role of long noncoding RNA RP5-881L22.5 as a novel biomarker and therapeutic target of colorectal cancer

BACKGROUND The incidence of colorectal cancer in humans is high,and it is in the top five for cancer-related morbidity and mortality.It is one of the main threats to human health.The function of long noncoding RNAs in tumor occurrence and development has gradually gained attention in recent years.In increasing numbers of studies,researchers have demonstrated that it plays an important role in the pathogenesis of colorectal cancer.AIM To find out if long noncoding RNA RP5-881L22.5 played a role in the pathogenesis of colorectal cancer in relation to the tumor microenvironment.METHODS We analyzed the transcriptome data and clinical data in The Cancer Genome Atlas-colon adenocarcinoma.The CIRBERSORT algorithm was applied to evaluate these tumor-infiltrating immune cells in The Cancer Genome Atlas-colon adenocarcinoma cancer tissue samples.Using the“estimate”package in R,we assessed the tumor immune microenvironment.The expression level of RP5-881L22.5 in tumor tissue and adjacent normal tissue samples from 4 pairs of colorectal cancer patients was determined by quantitative reverse transcription PCR.Colorectal cancer cells were tested for invasiveness using a transwell invasion assay after RP5-881L22.5 expression was knocked down.RESULTS The expression of lncRNA RP5-881L22.5 was related to the clinical characteristics of the tumors,and it was negatively related to the infiltration level of immune cells in the tumor microenvironment and the expression of T cell inhibitory receptors.A major function of its coexpressed mRNA was to regulate tumor immunity,such as the immune response.When quantitative reverse transcription PCR was performed on tumor tissues from 4 pairs of colorectal cancer patients,the results showed that RP5-881L22.5 was highly expressed.Subsequently,knocking down the expression of RP5-881L22.5,the invasiveness of colorectal cancer cell lines was reduced,and the apoptosis rate was increased.CONCLUSION RP5-881L22.5 plays a crucial role in the microenvironment of tumors as well as in the pathogenesis of colorectal cancer.The relationship between RP5-881L22.5 and the tumor immune microenvironment deserves further study.

基于遗传-启发算法的微动目标认知ISAR成像资源调度

认知逆合成孔径雷达(Inverse Synthetic Aperture Radar,ISAR)将认知雷达理论与ISAR成像技术相结合,利用目标与环境的离线先验知识及在线感知结果,提升ISAR在复杂环境下的成像能力.与传统单脉冲雷达相比较,相控阵ISAR可实现波束的快速扫描,因此具备多目标观测能力.在实际空间微动目标成像场景中,相控阵ISAR很可能存在多波束多目标情况.为实现系统效能的充分发挥,需要在系统能量、时间资源有限条件下实现成像任务有效调度.然而,面向成像任务的资源调度方法主要针对刚体目标,其约束条件及调度模型并不适用于微动目标,并且存在求解方法稳定性差、调度成功率不高等问题.此外,微动目标运动形式复杂,回波非平稳性很强,且方位缺损时难以聚焦成像.因此,迫切需要针对微动目标的特性,研究有效的ISAR资源调度和高分辨成像方法.针对上述问题,本文提出基于遗传-启发算法的微动目标认知ISAR成像资源调度方法 .该方法首先根据微动目标认知结果计算其成像所需的雷达资源及综合优先级;接着基于脉冲交错技术,在时间、能量双重约束下建立微动多目标资源调度模型.在此基础上,本文提出了基于遗传-启发算法的最优调度求解方法 .进一步,针对资源调度导致微动目标回波缺损,难以聚焦成像的问题,本文提出了基于交替方向乘子法(Alternating Direction Method of Multipliers,ADMM)的距离-瞬时多普勒(Range-Instantaneous Doppler,RID)成像方法,从而实现成功调度任务对应微动目标回波的高分辨聚焦成像.实验结果表明,相比于现有资源调度方法,本文所提方法调度结果更为稳定,调度成功率更高,并可实现回波缺损时的微动目标聚焦成像.

circSNRK通过上调Akt通路改善肾小管上皮细胞缺血-再灌注损伤

目的探讨环状RNA SNRK(circSNRK)在缺血-再灌注损伤(IRI)中的作用及机制。方法构建缺氧-复氧(IRI)细胞模型,检测IRI处理后circSNRK的表达情况及过表达circSNRK对细胞增殖和细胞凋亡的影响。分析circSNRK的作用靶点。将HK2细胞分为空白组(Mock组)、IRI组、对照质粒+IRI组(IRI+NC组)、过表达人circSNRK+IRI组(IRI+circSNRK组)、过表达人circSNRK+IRI+蛋白激酶B(Akt)抑制剂组(IRI+circSNRK+MK2206组)、对照质粒组(NC组)。检测Mock组、IRI组、IRI+NC组、IRI+circSNRK组细胞增殖及凋亡情况。进行circSNRK作用靶点的基因本体(GO)富集分析和京都基因与基因组百科全书(KEGG)聚类分析。检测Mock组、IRI组、IRI+NC组、IRI+circSNRK组细胞CDKN1A、Akt、B细胞淋巴瘤(Bcl)-2、半胱氨酸天冬氨酸蛋白酶(Caspase)-9信使RNA(mRNA)表达水平,p21、Bcl-2、Caspase-9、Akt、p-Akt蛋白表达水平。检测NC组、IRI+NC组、IRI+circSNRK组、IRI+circSNRK+MK2206组细胞增殖及凋亡情况。结果与Mock组比较,IRI组circSNRK表达水平较低,HK2细胞增殖能力下降,细胞凋亡增多。IRI+circSNRK组细胞增殖能力较IRI+NC组升高,细胞凋亡较IRI+NC组减少。circSNRK可通过51个微小RNA(miRNA)作用于648个靶点。GO富集分析显示,circSNRK作用靶点主要富集于细胞过程和生物调节等生物学过程,细胞部分、细胞和细胞外部分等细胞成分,以及结合、结合蛋白和酶等分子功能。KEGG聚类分析显示,circSNRK作用靶点主要富集在癌症信号通路、磷脂酰肌醇-3-激酶(PI3K)-Akt信号通路和癌症miRNA等相关通路。与Mock组比较,IRI组CDKN1A和Caspase-9 mRNA相对表达量较高,miR-99a-5p RNA表达水平较高,Akt和Bcl-2 mRNA相对表达量较低;与IRI+NC组比较,IRI+circSNRK组CDKN1A和Caspase-9 mRNA相对表达量较低,Akt和Bcl-2 mRNA相对表达量较高,miR-99a-5p RNA表达水平较低,差异均有统计学意义(均为P<0.05)。IRI组p21和Caspase-9蛋白表达较Mock组增多,p-Akt、Akt和Bcl-2蛋白表达较Mock组减少;IRI+circSNRK组p21和Caspase-9蛋白表达较IRI+NC组减少,p-Akt、Akt和Bcl-2蛋白表达较IRI+NC组增多。circSNRK和Akt上存在miR-99a-5p结合位点。与NC组比较,IRI+NC组细胞增殖能力下降;与IRI+NC组比较,IRI+circSNRK组细胞增殖能力升高;与IRI+circSNRK组比较,IRI+circSNRK+MK2206组细胞增殖能力下降(均为P<0.05)。IRI+NC组细胞凋亡水平较NC组高;IRI+circSNRK组细胞凋亡水平较IRI+NC组低;IRI+circSNRK+MK2206组细胞凋亡水平较IRI+circSNRK组高。结论在IRI条件下,circSNRK可影响HK2细胞增殖和凋亡,可能是通过Akt通路发挥作用。

基于改进Faster RCNN的微操作空间目标检测算法

将Faster RCNN引入微操作系统的目标检测之中。针对微操作空间下待检测目标存在尺度变化和在显微镜放大倍数较小时,待检测目标尺度过小、特征不明显的问题,提出了一种基于改进Faster RCNN的微操作空间目标检测算法。使用在图像分类任务中性能优越的深度残差网络提取图像的特征。引入递归特征金字塔网络,对特征进行融合。改进区域建议网络的采样策略,对损失函数进行优化。实验结果表明:这种改进的Faster RCNN算法能有效解决由于目标尺度变化和目标尺度过小带来的问题。相比通用的目标检测算法,该算法的准确度更高,速度更快,具有实际应用价值。

OFD-LFM MIMO雷达中旋转目标微多普勒效应分析及三维微动特征提取

该文将微多普勒效应引入到多输入多输出(MIMO)雷达技术研究,以旋转运动目标为例,分析了雷达辐射正交频分线性调频信号(OFD-LFM)时目标的微多普勒效应,给出了其参数化表达。在此基础上,进一步将微多普勒理论从目前的雷达视线方向上的微动分量提取扩展到微动部件3维运动和结构特征提取,利用MIMO雷达的多视角特性,提出了构建多元非线性方程组求解旋转部件的3维运动参数的算法,实现了目标3维微动特征的提取。仿真实验验证了算法的有效性和鲁棒性。