A single molecule detection technique was developed by the combination of a single channel poly （dimethylsiloxane）/glass micro-fluidic chip and fluorescence correlation spectroscopy （FCS）. This method was successf...A single molecule detection technique was developed by the combination of a single channel poly （dimethylsiloxane）/glass micro-fluidic chip and fluorescence correlation spectroscopy （FCS）. This method was successfully used to determine the proportion of two model components in the mixture containing fluorescein and the rhodamine-green succinimidyl ester.展开更多
The characteristics such as signal noise ratio（SNR） and sensitivity of the fluorescence detection system for micro-fluidic chip influence the performance of the whole system extremely. The confocal laser induced flu...The characteristics such as signal noise ratio（SNR） and sensitivity of the fluorescence detection system for micro-fluidic chip influence the performance of the whole system extremely. The confocal laser induced fluorescence detection system is presented. Based on the debugging of optical and circuit modules, the results of detecting the samples are given and analyzed theoretically, and the improved project is put forward.展开更多
In the research of bio-molecular chips and sensors, extra electric biases are most often employed to control and manipulate the DNA and protein molecules moving through micro/nano-fluidic channels. In order to accurat...In the research of bio-molecular chips and sensors, extra electric biases are most often employed to control and manipulate the DNA and protein molecules moving through micro/nano-fluidic channels. In order to accurately and flexibly control the bio-molecules as they move within the channels, a clear understanding of how the current changes within the buffer solution caused by an applied bias is fundamental. In this report, the current changed value of different buffer solutions, e.g., KC1, TE, and TBE was systematically studied with real-time monitoring and quantitative analysis in the situation of the buffers moving through a fluidic channel with a 5 μm inner diameter, driven by biases of 50 or 100 mV. The results revealed that the relation- ship between the current changed value and the pause interval of the applied electric field is highly consistent with the Hill Equation, which is helpful for accurately detecting and manipulating single biomolecules in microfluidic sensors and biochips.展开更多
The inclined micro-fluidized bed(MFB)can enhance heat and mass transfer rates compared to the vertically aligned counterparts,but the increased significance of surface forces and wall effects may cause poor fluidizati...The inclined micro-fluidized bed(MFB)can enhance heat and mass transfer rates compared to the vertically aligned counterparts,but the increased significance of surface forces and wall effects may cause poor fluidization performance.In this paper,the effects of column inclination and different particle-to-bed ratios(d_(P)/d_(B))on the solid hydrodynamics are investigated in an inclined micro-fluidized bed.The results validated the suitability of using the Ergun equation to predict minimum fluidization velocities due to small deviations between 1.01 and 1.81 times the theoretical values,for a particle-to-bed ratio ranging from 0.025 to 0.165 at inclinations between 0°and 10°.Investigation into the effects on bed expansion behavior showed that the bed contracted with an increase in bed inclination.An unexpected observation during the bed expansion was the appearance of a secondary high voidage region and the appearance of strong circulation patterns with an increase in bed inclination.A detailed analysis of this phenomenon suggested the presence of a critical angle at 6°and 10°for the 85μm particles,4×4 mm bed cross-section and 165μm particles,1×1 mm bed cross-section,respectively.However,the liquid-solid back-mixing was observed due to the modified particle trajectories resulted in the disappearance of the high voidage region.This paper gives new insights into the micro-fluidization behavior in inclined beds thus contributing to the development of micro-fluidized beds and their future applications.展开更多
近年来,微流体变焦透镜的研究已展示出其在光学系统中的应用前景,其中基于介质上电润湿(Electrowet-ting on dielectric,EWOD)的微流体变焦透镜只需改变外加电压便能快速调节透镜焦距,并且具有尺寸小、结构灵活、功耗低、焦距调节范围...近年来,微流体变焦透镜的研究已展示出其在光学系统中的应用前景,其中基于介质上电润湿(Electrowet-ting on dielectric,EWOD)的微流体变焦透镜只需改变外加电压便能快速调节透镜焦距,并且具有尺寸小、结构灵活、功耗低、焦距调节范围广等许多突出优点而日益受到注目。在介绍EWOD机理的基础上,综述了目前基于EWOD的微流体变焦透镜的研究进展。展开更多
利用原位聚合法在玻璃微管道内制备阴离子交换型固相萃取(SPE)微柱,以NO-2 为分析对象,针对NaNO2 KI Luminol发光体系设计微流控芯片,并将SPE微柱与微流控芯片连接起来组建成带有SPE微柱的复合式微流控芯片。分析了SPE微柱对NO-2 的吸...利用原位聚合法在玻璃微管道内制备阴离子交换型固相萃取(SPE)微柱,以NO-2 为分析对象,针对NaNO2 KI Luminol发光体系设计微流控芯片,并将SPE微柱与微流控芯片连接起来组建成带有SPE微柱的复合式微流控芯片。分析了SPE微柱对NO-2 的吸附保留与富集作用,在复合式微流控芯片上,实现了NO-2的进样、分离富集和检测,通过漏点曲线和交换容量两种方法分析了SPE微柱的柱容量。为控制SPE微柱的最大进样体积提供有利保障,并实现了食品中NO-2 的在线分离富集与检测。展开更多
基金This work was financially supported by the National Natural Science Foundation of China. （No.20271033, 20335020, 90408014）.
文摘A single molecule detection technique was developed by the combination of a single channel poly （dimethylsiloxane）/glass micro-fluidic chip and fluorescence correlation spectroscopy （FCS）. This method was successfully used to determine the proportion of two model components in the mixture containing fluorescein and the rhodamine-green succinimidyl ester.
基金Key Science and Technology Project Tackled of Guangdong Province(B2050070)
文摘The characteristics such as signal noise ratio（SNR） and sensitivity of the fluorescence detection system for micro-fluidic chip influence the performance of the whole system extremely. The confocal laser induced fluorescence detection system is presented. Based on the debugging of optical and circuit modules, the results of detecting the samples are given and analyzed theoretically, and the improved project is put forward.
基金supported by the Major Research Plan of the National Natural Science Foundation of China(Grant No.91123030)the Interna-tional Cooperation Foundation of the National Science and Technology Major Project of the Ministry of Science and Technology of China(Grant No.2011DFA12220)the National Natural Science Foundation of China(Grant No.61378083)
文摘In the research of bio-molecular chips and sensors, extra electric biases are most often employed to control and manipulate the DNA and protein molecules moving through micro/nano-fluidic channels. In order to accurately and flexibly control the bio-molecules as they move within the channels, a clear understanding of how the current changes within the buffer solution caused by an applied bias is fundamental. In this report, the current changed value of different buffer solutions, e.g., KC1, TE, and TBE was systematically studied with real-time monitoring and quantitative analysis in the situation of the buffers moving through a fluidic channel with a 5 μm inner diameter, driven by biases of 50 or 100 mV. The results revealed that the relation- ship between the current changed value and the pause interval of the applied electric field is highly consistent with the Hill Equation, which is helpful for accurately detecting and manipulating single biomolecules in microfluidic sensors and biochips.
基金supported by Newcastle University(Gant No.LOC/150025720/400382711).
文摘The inclined micro-fluidized bed(MFB)can enhance heat and mass transfer rates compared to the vertically aligned counterparts,but the increased significance of surface forces and wall effects may cause poor fluidization performance.In this paper,the effects of column inclination and different particle-to-bed ratios(d_(P)/d_(B))on the solid hydrodynamics are investigated in an inclined micro-fluidized bed.The results validated the suitability of using the Ergun equation to predict minimum fluidization velocities due to small deviations between 1.01 and 1.81 times the theoretical values,for a particle-to-bed ratio ranging from 0.025 to 0.165 at inclinations between 0°and 10°.Investigation into the effects on bed expansion behavior showed that the bed contracted with an increase in bed inclination.An unexpected observation during the bed expansion was the appearance of a secondary high voidage region and the appearance of strong circulation patterns with an increase in bed inclination.A detailed analysis of this phenomenon suggested the presence of a critical angle at 6°and 10°for the 85μm particles,4×4 mm bed cross-section and 165μm particles,1×1 mm bed cross-section,respectively.However,the liquid-solid back-mixing was observed due to the modified particle trajectories resulted in the disappearance of the high voidage region.This paper gives new insights into the micro-fluidization behavior in inclined beds thus contributing to the development of micro-fluidized beds and their future applications.
文摘近年来,微流体变焦透镜的研究已展示出其在光学系统中的应用前景,其中基于介质上电润湿(Electrowet-ting on dielectric,EWOD)的微流体变焦透镜只需改变外加电压便能快速调节透镜焦距,并且具有尺寸小、结构灵活、功耗低、焦距调节范围广等许多突出优点而日益受到注目。在介绍EWOD机理的基础上,综述了目前基于EWOD的微流体变焦透镜的研究进展。
