Peri-implantitis has been defined as an inflamematory condition involving dental implants, surrounding mucosa and bone, which lose supporting bone. Although high success rates for endosseous implants have been reporte...Peri-implantitis has been defined as an inflamematory condition involving dental implants, surrounding mucosa and bone, which lose supporting bone. Although high success rates for endosseous implants have been reported, failures occur, and some implants are lost or removed. At least 10% of the failures have been suggested to be the result of peri-implantitis. One of the major causes of the peri-implantitis is the bacterial colonization of implant surfaces but additional risk factors such as periodontitis, poor oral hygiene, tobacco consumption, prepost operative therapies and genetic susceptibility should be considered. In the present study a real-time PCR bases assay was designed to detect and quantify red complex species, then used to investigate 307 periodontal pocket samples from 127 periodontitis patients and 180 controls. Results demonstrated a significant higher prevalence of red complex species and increased amount of Porphyromonas gingivalis and Treponema denticolain periodontal pocket of periodontitis. Since a higher risk of peri-implantitis occurs in periodontally affected patients, detection and treatment of bacteria is a fundamental objective to ensure dental implant survival.展开更多
Bioevolution is still a main puzzle and attracts many scientists to research on it. Here I present that organisms have two impor- tant properties, definite structure and self-reproduction. Based on the number and conn...Bioevolution is still a main puzzle and attracts many scientists to research on it. Here I present that organisms have two impor- tant properties, definite structure and self-reproduction. Based on the number and connection of the structural units, organisms can be di- vided into three groups, unicellular, particellular and polycellular organisms. It can be called polycellular evolution that organisms evolve from unicellular, particellular to polycellular. Also it can be called diploid evolution that organisms evolve from haplobes to diplobes, two groups based on the reproductive differences. Some concepts like spore, zygote and embryos are redefined in the paper. Moreover, I present that embryos are the most important boundary of bioevolution and organisms can be divided into two evolutionary phases, the lower and the higher. The lower organisms, Kingdom Microbia (kingd. nov.), are inembryonate, which include Acytophyla (phyl. nov.), bacteria, protozoa, fungi and inembryonate algae. The higher organisms are embryonate and have two branches, Kingdom Plantae and Kingdom Animalia. Plantae are sessile and, autotrophic or sporogenic, which include higher plants and Nudembryophyta (phyl. nov.). Animalia are heterotrophic and, motile or gametogenic, which include all multicellular animals. The new system, which reflects the two important phases of bioevolu- tion and two branches of higher organisms, can really correct the problem of different kingdoms in different researches or by different re- searchers.展开更多
Factors including Mg^2＋, dNTP and primer that affected RAPD were studied using orthogonal experimental design, and at the same time, anneal temperature, extending time and cycles were also studied. Finally, PCR react...Factors including Mg^2＋, dNTP and primer that affected RAPD were studied using orthogonal experimental design, and at the same time, anneal temperature, extending time and cycles were also studied. Finally, PCR reaction system that is feasible for black soil microbial PCR amplification was determined, containing 7 ng DNA template, 20 pM random primers, 1.5 U Taq DNA polymerase, 3.0 mM MgCl2 and 0.2 mmol ·L^-1 dNTP, with procedure： 40 cycles for 3 min at 94 ℃ （temperature）, 40 s at 37 ℃ （annealing）, 1.5 min at 72 ℃ （extension）, a final elongation step at 72 ℃ for 7 min.展开更多
文摘Peri-implantitis has been defined as an inflamematory condition involving dental implants, surrounding mucosa and bone, which lose supporting bone. Although high success rates for endosseous implants have been reported, failures occur, and some implants are lost or removed. At least 10% of the failures have been suggested to be the result of peri-implantitis. One of the major causes of the peri-implantitis is the bacterial colonization of implant surfaces but additional risk factors such as periodontitis, poor oral hygiene, tobacco consumption, prepost operative therapies and genetic susceptibility should be considered. In the present study a real-time PCR bases assay was designed to detect and quantify red complex species, then used to investigate 307 periodontal pocket samples from 127 periodontitis patients and 180 controls. Results demonstrated a significant higher prevalence of red complex species and increased amount of Porphyromonas gingivalis and Treponema denticolain periodontal pocket of periodontitis. Since a higher risk of peri-implantitis occurs in periodontally affected patients, detection and treatment of bacteria is a fundamental objective to ensure dental implant survival.
文摘Bioevolution is still a main puzzle and attracts many scientists to research on it. Here I present that organisms have two impor- tant properties, definite structure and self-reproduction. Based on the number and connection of the structural units, organisms can be di- vided into three groups, unicellular, particellular and polycellular organisms. It can be called polycellular evolution that organisms evolve from unicellular, particellular to polycellular. Also it can be called diploid evolution that organisms evolve from haplobes to diplobes, two groups based on the reproductive differences. Some concepts like spore, zygote and embryos are redefined in the paper. Moreover, I present that embryos are the most important boundary of bioevolution and organisms can be divided into two evolutionary phases, the lower and the higher. The lower organisms, Kingdom Microbia (kingd. nov.), are inembryonate, which include Acytophyla (phyl. nov.), bacteria, protozoa, fungi and inembryonate algae. The higher organisms are embryonate and have two branches, Kingdom Plantae and Kingdom Animalia. Plantae are sessile and, autotrophic or sporogenic, which include higher plants and Nudembryophyta (phyl. nov.). Animalia are heterotrophic and, motile or gametogenic, which include all multicellular animals. The new system, which reflects the two important phases of bioevolu- tion and two branches of higher organisms, can really correct the problem of different kingdoms in different researches or by different re- searchers.
基金This Work was Supported by the National Natural Science Foundation of China (30230250, 30370819) Heilongjiang ProvinceScience Foundation (C0215)
文摘Factors including Mg^2＋, dNTP and primer that affected RAPD were studied using orthogonal experimental design, and at the same time, anneal temperature, extending time and cycles were also studied. Finally, PCR reaction system that is feasible for black soil microbial PCR amplification was determined, containing 7 ng DNA template, 20 pM random primers, 1.5 U Taq DNA polymerase, 3.0 mM MgCl2 and 0.2 mmol ·L^-1 dNTP, with procedure： 40 cycles for 3 min at 94 ℃ （temperature）, 40 s at 37 ℃ （annealing）, 1.5 min at 72 ℃ （extension）, a final elongation step at 72 ℃ for 7 min.
文摘目的分析胎盘微生物定植情况,探讨胎盘拭子培养在新生儿早发败血症(early onset sepsis,EOS)诊断及治疗中的价值,为临床合理用药提供参考。方法选取2015年5月至2020年5月胎盘拭子培养阳性的孕妇858例。将母亲胎盘培养阳性且新生儿诊断EOS的64例病例纳入EOS组,选取同期母亲胎盘培养阳性,但未诊断EOS新生儿为非EOS组,按1∶4配对病例对照研究,共256例纳入非EOS组。分析胎盘拭子培养的病原体分布情况、病原体常用药物敏感试验结果、胎盘拭子培养阳性与否和EOS的相关性以及两组患儿一般情况、围产期相关危险因素等。结果研究期间胎盘拭子培养阳性858例(9.0%)。共有13种病原体,295例(34.4%)革兰氏阳性菌、504例(58.7%)革兰氏阴性菌、53例(6.2%)真菌、6例(0.7%)混合菌。胎盘拭子培养前3位病原体依次为:大肠埃希菌(51.6%)、肠球菌属(16.3%)、B族溶血性链球菌(group B streptococcus,GBS,10.4%)。EOS组和非EOS组在患儿胎龄、出生体重、性别、分娩方式方面差异无统计学意义(P>0.05)。EOS组前3位病原体分别依次为:大肠埃希菌(50.0%)、肠球菌属(20.3%)及GBS(14.1%)。大肠埃希菌对哌拉西林他唑巴坦和美罗培南敏感性均高(分别为98.0%、99.8%),对头孢噻肟的敏感率为76.1%,63.3%对氨苄西林耐药;肠球菌属对氨苄西林(100%)、万古霉素(100%)均敏感;GBS对青霉素(100%)、万古霉素(100%)均敏感,63.0%对红霉素耐药。胎盘拭子培养阳性与否和EOS的发生无统计学意义(P>0.05)。对于胎盘GBS定植,GBS-EOS发病率高于大肠埃希菌和肠球菌属。母亲合并绒毛膜羊膜炎是胎盘拭子培养阳性伴发EOS的独立危险因素(P=0.001,OR=2.503,95%CI:1.47~4.27)。结论胎盘拭子培养阳性与否与EOS的发生无明显相关性,但孕母合并绒毛膜羊膜炎是EOS的独立危险因素,应给以及时评估,早期合理应用抗生素。
