For decades,manufacturers have boasted about how small they can make microchip components.Transistors have shrunk by about 1000-fold over the last 50 years,for example[1].But Cerebras Systems,Inc.of Sunnyvale,CA,USA t...For decades,manufacturers have boasted about how small they can make microchip components.Transistors have shrunk by about 1000-fold over the last 50 years,for example[1].But Cerebras Systems,Inc.of Sunnyvale,CA,USA takes pride in how big its chips are.Produced from a single silicon wafer,its Wafer-Scale Engine(WSE)-2 chips measure 46225 mm^(2),56 times the size of a standard Nvidia microprocessor(Fig.1)[2].展开更多
Gene synthesis has provided important contributions in various fields including genomics and medicine. Current genes are 7 - 30 cents depending on the assembly and sequencing methods performed. Demand for gene synthes...Gene synthesis has provided important contributions in various fields including genomics and medicine. Current genes are 7 - 30 cents depending on the assembly and sequencing methods performed. Demand for gene synthesis has been increasing for the past few decades, yet available methods remain expensive. A solution to this problem involves microchip-derived oligonucleotides (oligos), an oligo pool with a substantial number of oligo fragments. Microchips have been proposed as a tool for gene synthesis, but this approach has been criticized for its high error rate during sequencing. This study tests a possible cost-effective method for gene synthesis utilizing fragment assembly and golden gate assembly, which can be employed for quicker manufacturing and efficient execution of genes in the near future. The droplet method was tested in two trials to determine the viability of the method through the accuracy of the oligos sequenced. A preliminary research experiment was performed to determine the efficacy of oligo lengths ranging from two to four overlapping oligos through Gibson assembly. Of the three oligo lengths tested, only two fragment oligos were correctly sequenced. Two fragment oligos were used for the second experiment, which determined the efficacy of the droplet method in reducing gene synthesis cost and speed. The first trial utilized a high-fidelity polymerase and resulted in 3% correctly sequenced oligos, so the second trial utilized a non-high-fidelity polymerase, resulting in 8% correctly sequenced oligos. After calculating, the cost of gene synthesis lowers down to 0.8 cents/base. The final calculated cost of 0.8 cents/base is significantly cheaper than other manufacturing costs of 7 - 30 cents/base. Reducing the cost of gene synthesis provides new insight into the cost-effectiveness of present technologies and protocols and has the potential to benefit the fields of bioengineering and gene therapy.展开更多
随着安全威胁的不断演变和日趋复杂,工业和消费应用设计人员在开发过程中必须考虑在设备中实现安全功能。为了让设计人员能轻松地将安全功能集成到应用中,Microchip Technology Inc.(美国微芯科技公司)宣布推出全新PIC32CZ CA 32位单片...随着安全威胁的不断演变和日趋复杂,工业和消费应用设计人员在开发过程中必须考虑在设备中实现安全功能。为了让设计人员能轻松地将安全功能集成到应用中,Microchip Technology Inc.(美国微芯科技公司)宣布推出全新PIC32CZ CA 32位单片机系列。该系列器件配备了300 MHz Arm Cortex M7处理器、集成硬件安全模块(HSM),支持多种连接和闪存选项,从而提高了灵活性。展开更多
文摘For decades,manufacturers have boasted about how small they can make microchip components.Transistors have shrunk by about 1000-fold over the last 50 years,for example[1].But Cerebras Systems,Inc.of Sunnyvale,CA,USA takes pride in how big its chips are.Produced from a single silicon wafer,its Wafer-Scale Engine(WSE)-2 chips measure 46225 mm^(2),56 times the size of a standard Nvidia microprocessor(Fig.1)[2].
文摘Gene synthesis has provided important contributions in various fields including genomics and medicine. Current genes are 7 - 30 cents depending on the assembly and sequencing methods performed. Demand for gene synthesis has been increasing for the past few decades, yet available methods remain expensive. A solution to this problem involves microchip-derived oligonucleotides (oligos), an oligo pool with a substantial number of oligo fragments. Microchips have been proposed as a tool for gene synthesis, but this approach has been criticized for its high error rate during sequencing. This study tests a possible cost-effective method for gene synthesis utilizing fragment assembly and golden gate assembly, which can be employed for quicker manufacturing and efficient execution of genes in the near future. The droplet method was tested in two trials to determine the viability of the method through the accuracy of the oligos sequenced. A preliminary research experiment was performed to determine the efficacy of oligo lengths ranging from two to four overlapping oligos through Gibson assembly. Of the three oligo lengths tested, only two fragment oligos were correctly sequenced. Two fragment oligos were used for the second experiment, which determined the efficacy of the droplet method in reducing gene synthesis cost and speed. The first trial utilized a high-fidelity polymerase and resulted in 3% correctly sequenced oligos, so the second trial utilized a non-high-fidelity polymerase, resulting in 8% correctly sequenced oligos. After calculating, the cost of gene synthesis lowers down to 0.8 cents/base. The final calculated cost of 0.8 cents/base is significantly cheaper than other manufacturing costs of 7 - 30 cents/base. Reducing the cost of gene synthesis provides new insight into the cost-effectiveness of present technologies and protocols and has the potential to benefit the fields of bioengineering and gene therapy.
文摘随着安全威胁的不断演变和日趋复杂,工业和消费应用设计人员在开发过程中必须考虑在设备中实现安全功能。为了让设计人员能轻松地将安全功能集成到应用中,Microchip Technology Inc.(美国微芯科技公司)宣布推出全新PIC32CZ CA 32位单片机系列。该系列器件配备了300 MHz Arm Cortex M7处理器、集成硬件安全模块(HSM),支持多种连接和闪存选项,从而提高了灵活性。
