Jianpi-Huatan-Huoxue-Anshen formula ameliorates gastrointestinal inflammation and microecological imbalance in chemotherapytreated mice transplanted with H22 hepatocellular carcinoma

BACKGROUND Jianpi-Huatan-Huoxue-Anshen formula[Tzu-Chi cancer-antagonizing&lifeprotecting II decoction(TCCL)]is a Chinese medical formula that has been clinically shown to reduce the gastrointestinal side effects of chemotherapy in cancer patients and improve their quality of life.However,its effect and mechanism on the intestinal microecology after chemotherapy are not yet clear.AIM To discover the potential mechanisms of TCCL on gastrointestinal inflammation and microecological imbalance in chemotherapy-treated mice transplanted with hepatocellular carcinoma(HCC).METHODS Ninety-six mice were inoculated subcutaneously with HCC cells.One week later,the mice received a large dose of 5-fluorouracil by intraperitoneal injection to establish a HCC chemotherapy model.Thirty-six mice were randomly selected before administration,and feces,ileal tissue,and ileal contents were collected from each mouse.The remaining mice were randomized into normal saline,continuous chemotherapy,Yangzheng Xiaoji capsulestreated,and three TCCL-treated groups.After treatment,feces,tumors,liver,spleen,thymus,stomach,jejunum,ileum,and colon tissues,and ileal contents were collected.Morphological changes,serum levels of IL-1β,IL-6,IL-8,IL-10,IL-22,TNF-α,and TGF-β,intestinal SIgA,and protein and mRNA expression of ZO-1,NF-κB,Occludin,MUC-2,Claudin-1,and IκB-αin colon tissues were documented.The effect of TCCL on the abundance and diversity of intestinal flora was analyzed using 16S rDNA sequencing.RESULTS TCCL treatment improved thymus and spleen weight,thymus and spleen indexes,and body weight,decreased tumor volumes and tumor tissue cell density,and alleviated injury to gastric,ileal,and colonic mucosal tissues.Among proteins and genes associated with inflammation,IL-10,TGF-β,SIgA,ZO-1,MUC-2,and Occludin were upregulated,whereas NF-κB,IL-1β,IL-6,TNF-α,IL-22,IL-8,and IκB-αwere downregulated.Additionally,TCCL increased the proportions of fecal Actinobacteria,AF12,Adlercreutzia,Clostridium,Coriobacteriaceae,and Paraprevotella in the intermediate stage of treatment,decreased the proportions of Mucipirillum,Odoribacter,RF32,YS2,and Rikenellaceae but increased the proportions of p_Deferribacteres and Lactobacillus at the end of treatment.Studies on ileal mucosal microbiota showed similar findings.Moreover,TCCL improved community richness,evenness,and the diversity of fecal and ileal mucosal flora.CONCLUSION TCCL relieves pathological changes in tumor tissue and chemotherapy-induced gastrointestinal injury,potentially by reducing the release of pro-inflammatory factors to repair the gastrointestinal mucosa,enhancing intestinal barrier function,and maintaining gastrointestinal microecological balance.Hence,TCCL is a very effective adjuvant to chemotherapy.

Intestinal microecological transplantation for a patient with chronic radiation enteritis:A case report

BACKGROUND The gut microbiota is strongly associated with radiation-induced gut damage.This study aimed to assess the effectiveness and safety of intestinal microecological transplantation for treating patients with chronic radiation enteritis.CASE SUMMARY A 64-year-old female with cervical cancer developed abdominal pain,diarrhea,and blood in the stool 1 year after radiotherapy.An electronic colonoscopy was performed to diagnose chronic radiation enteritis.Two courses of intestinal microecological transplantation and full-length 16S rRNA microbiological analysis were performed.The patient experienced short-and long-term relief from symptoms without adverse effects.Whole 16S rRNA sequencing revealed significant differences in the intestinal flora’s composition between patient and healthy donors.Pathogenic bacteria,such as Escherichia fergusonii and Romboutsia timonensis,were more in the patient.Beneficial bacteria such as Faecalibacterium prausnitzii,Fusicatenibacter saccharivorans,Ruminococcus bromii,and Bifidobacterium longum were more in the healthy donors.Intestinal microbiota transplantation resulted in a significant change in the patient's intestinal flora composition.The composition converged with the donor's flora,with an increase in core beneficial intestinal bacteria,such as Eubacterium rectale,and a decrease in pathogenic bacteria.Changes in the intestinal flora corresponded with the patients'alleviating clinical symptoms.CONCLUSION Intestinal microecological transplantation is an effective treatment for relieving the clinical symptoms of chronic radiation enteritis by altering the composition of the intestinal flora.This study provides a new approach for treating patients with chronic radiation enteritis.

PRELIMINARY STUDY ON INCREASING GROWTH AND REJUVENATION FOR OSSIFIED EELS WITH THE MICROECOLOGICS

This paper was adopting intestinal normal microbiota: Micrococcus tntestinalt, sp. and Beneckea campbell, and were prepared to microecologics, also gave a rebuild of intestinal micro ecospecies for ossified eels, and research effect of increasing growth and rejuvenation to them, by the microecologics. Moreover, specially to set up double-control, including a thyroideum medicament and an empty test group, for the comparison and analysis. Our results show that the intestinal microecologics of eel, for increasing growth and rejuvenation to ossified eels, that are botth achieve striking effect. But looked the thyroideum for increasing growth to ossified eels were be very effective. Nevertheless, which for rejuvenation was failed to take effect.Furthermore, we inc-lined to believe that the could be possible still significance of effect, if again added intestinal obligate anserobes of adult eels, and the cerebiogen into this microecologics.

Effects of Lactobacillus Microecologics on Production Performance, Immune Function and Intestinal Microflora of Egg Chicks

[Objective]The paper was to study the effects of lactobacillus microecologics on production performance,immune function and intestinal microflora of egg chicks.[Method]Totally 18001-day-old chicks of Hyline brown were randomly divided into three groups,four repeats each group and 150 chicks each repeat.The chicks in control group were supplied with basic diet,and those in experimental groups I and II were supplied with the basic diet added with 100 and 150 g/t microecologics,respectively.[Result]Compared with the control group,the daily gain of egg chicks in experimental group II after 7 weeks was increased by 3.67%(P<0.05),and the feed conversion ratio was significantly reduced by 0.05(P<0.05);the thymus indexes in experimental groups I and II were significantly improved by 4.95%and 5.94%(P<0.05),and the spleen indexes were significantly improved by 3.32% and 4.36% compared with the control group(P<0.05).The index of bursa of fabricius in experimental group II was extremely improved by 9.66%compared with the control group(P<0.01).Meantime,experimental group II extremely improved the number of living lactobacillus in caecum of egg chicks(P<0.01),and extremely reduced the number of Escherichia coli in caecum(P<0.01).[Conclusion]Lactobacillus microecologics are beneficial to the improvement of production performance,immune function and intestinal flora of egg chicks.The optimal addition amount in the feed of chicks is 150 g/t.

Application of Animal Microecological Preparation in Pig Production

Microecological preparation is an ideal antibiotic and growth-promoting hormone alternative with no toxicity, residue or pollution. This study aimed to analyze the concept, selection requirements, functional action and application in pig production of microecological preparation probiotics, prebiotics and synbiotics, and investigate the hot spot and prospects for development of animal microecological preparation.

Discussion on Periodontal Microecological Health

Gum during the onset of inflammation and swelling, and accompanied by serious plaque accumulation, and extended from the gums to the lower part of the gums. Because of the characteristics of the lower gingival micro-ecological environment, gingival plaque and the emergence of a large number of pathogenic bacteria, resulting in exacerbations of periodontitis and have a tendency to expand, resulting in alveolar bone resorption and periodontal pocket, the formation of various types Of periodontitis.

Microecological Prevention and Control Technology of Facility Strawberry Continuous Cropping Diseases and Its Application Effects

In order to actively develop green ecological strawberry, the authors have studied integrated microecological prevention and control technologies of strawberry continuous cropping diseases, including increasing soil biomass and solar high temperature disinfection during strawberry greenhouse leisure period in summer, biological fertilizer (agent) treatment before colonization, root irrigation treatment with biocontrol agent, as well as appropriate amount of fulvic acid and cytex after colonization, forming a supporting technical system for the microecological prevention and control of soil-borne diseases in facilities. The demonstration application in production shows that the technology has the functions of restoring and enhancing soil biological fertility, enriching beneficial microbial flora, antagonizing harmful bacteria, significantly promoting the growth and development of strawberry, reducing the incidence of soil-borne diseases, reducing the use of chemical fertilizers and pesticides, promoting the early ripening and high yield of strawberry, significantly improving the quality and flavor of strawberry, reducing the risk of pesticide residues, and boosting consumer confidence, which further improves the economic benefit, social benefit and ecological benefit, with good application prospect in production.

Intestinal Microecology in Children with Pneumonia: The Relationship Between Digestive Health and Disease Recovery

This paper explores the association between intestinal microecology and digestive health and disease recovery in children with pneumonia.Intestinal microecological imbalance is common in children with pneumonia,which is closely associated with digestive health and disease recovery.Intestinal microecological imbalance may affect digestive enzyme activity,intestinal mucosal barrier function,and nutrient absorption,which in turn affects digestive health.In addition,intestinal microecological imbalances may be associated with immune regulation,inflammatory responses,and pathogen suppression,affecting disease recovery.Strategies to regulate intestinal microecology include probiotic supplementation,dietary modification,and pharmacological treatment.Currently,the study of intestinal microecology in children with pneumonia faces challenges,and there is a need for improved research methods,individualized treatment strategies,and the development of novel probiotics.In conclusion,the intestinal microecology of children with pneumonia is closely related to digestive health and disease recovery,and the regulation of intestinal microecology is of great significance to the treatment of children with pneumonia.Furthermore,future research should further explore the application of the microecology of the intestinal microecology in the treatment of children with pneumonia.

The Latest Research Progress on Application of Lactobacillus acidophilus

Lactobacillus acidophilus belongs to the genus Lactobacillus, Gram-positive bacteria, which mainly colonize the small intestine of animals, can secrete anti-biotin substances, inhibit the growth and reproduction of some intestinal pathogenic bacteria, thereby regulating Intestinal flora, strengthen the absorption and utilization of feed nutrients, improve resistance, and promote animal growth, so it is widely used in livestock and poultry production. This article summarizes the latest research progress and application of Lactobacillus acidophilus, and looks forward to the future development direction in order to provide a theoretical basis for its future research.

Exploring choices of early nutritional support for patients with sepsis based on changes in intestinal microecology

BACKGROUND Sepsis exacerbates intestinal microecological disorders leading to poor prognosis.Proper modalities of nutritional support can improve nutrition,immunity,and intestinal microecology.AIM To identify the optimal modality of early nutritional support for patients with sepsis from the perspective of intestinal microecology.METHODS Thirty patients with sepsis admitted to the intensive care unit of the General Hospital of Ningxia Medical University,China,between 2019 and 2021 with indications for nutritional support,were randomly assigned to one of three different modalities of nutritional support for a total of 5 d:Total enteral nutrition(TEN group),total parenteral nutrition(TPN group),and supplemental parenteral nutrition(SPN group).Blood and stool specimens were collected before and after nutritional support,and changes in gut microbiota,short-chain fatty acids(SCFAs),and immune and nutritional indicators were detected and compared among the three groups.RESULTS In comparison with before nutritional support,the three groups after nutritional support presented:(1)Differences in the gut bacteria(Enterococcus increased in the TEN group,Campylobacter decreased in the TPN group,and Dialister decreased in the SPN group;all P<0.05);(2)different trends in SCFAs(the TEN group showed improvement except for Caproic acid,the TPN group showed improvement only for acetic and propionic acid,and the SPN group showed a decreasing trend);(3)significant improvement of the nutritional and immunological indicators in the TEN and SPN groups,while only immunoglobulin G improved in the TPN group(all P<0.05);and(4)a significant correlation was found between the gut bacteria,SCFAs,and nutritional and immunological indicators(all P<0.05).CONCLUSION TEN is recommended as the preferred mode of early nutritional support in sepsis based on clinical nutritional and immunological indicators,as well as changes in intestinal microecology.

Research progress on the relationship between Paneth cellssusceptibility genes,intestinal microecology and inflammatory bowel disease

Inflammatory bowel disease(IBD)is a disorder of the immune system and intestinal microecosystem caused by environmental factors in genetically susceptible people.Paneth cells(PCs)play a central role in IBD pathogenesis,especially in Crohn's disease development,and their morphology,number and function are regulated by susceptibility genes.In the intestine,PCs participate in the formation of the stem cell microenvironment by secreting antibacterial particles and play a role in helping maintain the intestinal microecology and intestinal mucosal homeostasis.Moreover,PC proliferation and maturation depend on symbiotic flora in the intestine.This paper describes the interactions among susceptibility genes,PCs and intestinal microecology and their effects on IBD occurrence and development.

Rifaximin in the Treatment of Gastroesophageal Reflux Disease: A New Idea Based on the Relationship between Intestinal Microecology and Gastroesophageal Reflux Disease

Rifaximin in the Treatment of Gastroesophageal Reflux Disease (GERD) is a common clinical disorder, the most common symptom of which is a burning sensation behind the breastbone (heartburn) or reflux of stomach contents into the upper pharynx (acid reflux). The prevalence in China is increasing year by year, which can affect the quality of life of patients and also increase the economic burden on families and society. The pathogenesis of GERD is still unclear, and some studies suggest that intestinal microecology may be closely related to the development of GERD. Rifaximin is not readily absorbed orally and acts locally in the intestine, so it has mild adverse effects and good safety, and can be used to treat gastrointestinal diseases such as irritable bowel syndrome, traveler’s diarrhea, small intestinal bacterial overgrowth, diverticulosis, inflammatory bowel disease and hepatic encephalopathy. Therefore, this paper focuses on intestinal microecology as a possible pathogenesis of GERD and further explores the feasibility of rifaximin for the treatment of GERD.

Research progress on the correlation between gestational vaginal microecology and vaginal infectious diseases

The health status of the vaginal microenvironment, a complicated system, is an important indicator of female reproductive health. The vaginal flora is in a state of balance, and the microorganisms coexist and are interdependent to maintain the vaginal micro-ecological balance, which is a kind of dynamic balance influenced by endogenous and exogenous factors. Vaginal infections are traditionally treated by killing microbes in the vagina. Given the extensive study of the internal vaginal environment, people become gradually aware of the significance of maintaining the vaginal micro-ecological balance rather than blindly using antimicrobial agents. The balance in the vaginal internal environment is disrupted during the gestation period as the secretion of progesterone increases. The imbalanced vaginal micro-ecological environment may lead to vaginal infectious diseases. This paper provides a review of the relationship between the vaginal microecology and infectious diseases during gestation period.

Thinking on the Treatment of Lung Cancer by Regulating Lung-Intestinal Axis Microecology by Traditional Chinese Medicine

The intestinal microecological disorder is closely related to the occurrence and development of lung cancer and the treatment response.This paper starts with the relationship between the lungintestinal axis,to discuss the possibility of TCM regulating the target of intestinal microecological treatment of lung cancer,so as to provide new research ideas for TCM treatment of lung cancer.

Research progress on pathogenesis of ulcerative colitis

The incidence of ulcerative colitis is increasing year by year,yet the pathogenesis is still not clear.Many scholars have studied the genetic factors,environmental factors,intestinal microecological imbalance,intestinal mucosal barrier disorder,abnormal immune response and mitochondrial diseases,and abundant achievements have been made.In order to further understand the possible pathogenesis of ulcerative colitis,this paper reviews its research progress,in order to better guide clinical medication,and provide new ideas for further study of its pathogenesis.

Analysis of Vagina Microecology and Investigation of Common Infection in Border Area of Chongzuo City

Objective: To investigate the microecology and common infection of the female vagina in border area of Chongzuo City. Methods: Female patients in the outpatient department and inpatient department of gynecology of our hospital from April 1, 2018 to August 23, 2021 were selected as the research objects. The reproductive tract secretions of all patients were examined by microecological examination, morphological examination, chemical analysis and microscopic examination, and the results of various indicators were statistically analyzed to draw relevant conclusions. Results: Among the 1498 women with vaginal micro- ecological imbalances, cleanliness was determined to be 534 cases of degree II, 868 cases of degree III, and 96 cases of degree IV;Nugent scores were 9 - 10 in 9 cases, 8 in 122 cases, 7 in 30 cases, 6 in 18 cases, 5 in 8 cases, 4 in 717 cases, 3 in 248 cases, 2 in 209 cases, 1 in 118 cases, and 0 in 19 cases. AV scores were 8 in 1 case, 7 in 1 case, 6 in 2 cases, 5 in 9 cases, 4 in 84 cases, 3 in 207 cases, 2 in 850 cases, 1 in 284 cases, and 0 in 60 cases. Among the pathogen infections, 99 cases were mycoplasma, 361 cases were candida, 199 cases were bacterial and 8 cases were trichomonas;In chemical analysis, there were 138 cases with pH 5.1, 326 cases with pH 4.8, 775 cases with pH 4.6, 217 cases with pH 4.4, and 42 cases with pH 4.1;meanwhile, 1390 cases were positive for hydrogen peroxide, 505 cases were positive for glucosidase, 487 cases were positive for acetylglucosaminidase, 184 cases were positive for sialidase, 290 cases were positive for proline amino-peptidase, 501 cases were positive for coagulase, and 1064 cases were positive for leukocyte esterase. Conclusion: Female reproductive tract microecology is more complex, and the pathogens of infection are also diverse. A systematic examination can clarify the occurrence and development of the disease, and then provide reliable laboratory data for clinical treatment and guide clinical medication.

A review of research progress on continuous cropping obstacles

Due to the increasing global population and limited land resources, continuous cropping has become common. However, after a few years of continuous cropping, obstacles often arise that cause soil degeneration, decreased crop yield and quality, and increased disease incidence, resulting in significant economic losses. It is essential to understand the causes and mitigation mechanisms of continuous cropping obstacles(CCOs) and then develop appropriate methods to overcome them. This review systematically summarizes the causes and mitigation measures of soil degradation in continuous cropping through a meta-analysis. It was concluded that not all continuous cropping systems are prone to CCOs. Therefore, it is necessary to grasp the principles governing the occurrence of diseases caused by soilborne pathogens in different cropping systems, consider plant–soil-organisms interactions as a system, scientifically regulate the physical and chemical properties of soils from a systems perspective, and then regulate the structure of microbial food webs in the soil to achieve a reduction in diseases caused by soilborne pathogens and increase crop yield ultimately. This review provides reference data and guidance for addressing this fundamental problem.

The gut-eye axis:from brain neurodegenerative diseases to age-related macular degeneration

Age-related macular degeneration is a serious neurodegenerative disease of the retina that significantly impacts vision.Unfortunately,the specific pathogenesis remains unclear,and effective early treatment options are consequently lacking.The microbiome is defined as a large ecosystem of microorganisms living within and coexisting with a host.The intestinal microbiome undergoes dynamic changes owing to age,diet,genetics,and other factors.Such dysregulation of the intestinal flora can disrupt the microecological balance,resulting in immunological and metabolic dysfunction in the host,and affecting the development of many diseases.In recent decades,significant evidence has indicated that the intestinal flora also influences systems outside of the digestive tract,including the brain.Indeed,several studies have demonstrated the critical role of the gut-brain axis in the development of brain neurodegenerative diseases,including Alzheimer’s disease and Parkinson’s disease.Similarly,the role of the“gut-eye axis”has been confirmed to play a role in the pathogenesis of many ocular disorders.Moreover,age-related macular degeneration and many brain neurodegenerative diseases have been shown to share several risk factors and to exhibit comparable etiologies.As such,the intestinal flora may play an important role in age-related macular degeneration.Given the above context,the present review aims to clarify the gut-brain and gut-eye connections,assess the effect of intestinal flora and metabolites on age-related macular degeneration,and identify potential diagnostic markers and therapeutic strategies.Currently,direct research on the role of intestinal flora in age-related macular degeneration is still relatively limited,while studies focusing solely on intestinal flora are insufficient to fully elucidate its functional role in age-related macular degeneration.Organ-on-a-chip technology has shown promise in clarifying the gut-eye interactions,while integrating analysis of the intestinal flora with research on metabolites through metabolomics and other techniques is crucial for understanding their potential mechanisms.

Biochar-based Bacillus subtilis inoculant for enhancing plants disease prevention:microbiota response and root exudates regulation

Plants regulate root exudates to form the composition of rhizosphere microbial community and resist disease stress.Many studies advocate intervention with biochar(BC)and exogenous microbe to enhance this process and improve plant defenses.However,the mechanism by which BC mediates exogenous microorganisms to enhance root exudate-soil microbial defensive feedback remains unclear.Here,a BC-based Bacillus subtilis SL-44 inoculant(BC@SL)was prepared to investigate the defensive feedback mechanism for plants,which enhanced plant growth and defense more than BC or SL-44 alone.BC@SL not only strengthened the direct inhibition of Rhizoctonia solani Rs by solving the problem of reduced viability of a single SL-44 inoculant but also indirectly alleviated the Rs stress by strengthening plant defensive feedback,which was specifically manifested by the following:(1)increasing the root resistance enzyme activities(superoxide dismutase up to 3.5 FC);(2)increasing the abundance of beneficial microbe in soil(0.38-16.31%Bacillus);and(3)remodeling the composition of root exudates(palmitic acid 3.95-6.96%,stearic acid 3.56-5.93%,2,4 tert-butylphenol 1.23-2.62%,increasing citric acid 0.94-1.81%,and benzoic acid 0.97-2.13%).The mechanism reveals that BC@SL can enhance the positive regulatory effect between root exudates and microorganisms by optimizing their composition.Overall,BC@SL is a stable and efficient new solid exogenous soil auxiliary,and this study lays the foundation for the generalization and application of green pesticides.

Regenerative Medicine-Opportunities and Challenges

In this paper, the definitions and contents of regenerative medicine, including tissue engineering, cell therapy, cytokiee therapy and microecologic cure, were presented. And then, the prospacts and difficulties for clinical application of these therapies were further mentioned. It indicated that the regenerative medicine came to a new era with full of opportunities and challenges. To face and seize this evanescent opportunity is worthy of detailed discussions.