Buried straw layer and plastic mulching increase microflora diversity in salinized soil

Salt stress has been increasingly constraining crop productivity in arid lands of the world. In our recent study, salt stress was aleviated and crop productivity was improved remarkably by straw layer burial plus plastic iflm mulching in a saline soil. However, its impact on the microlfora diversity is not wel documented. Field micro-plot experiments were conducted from 2010 to 2011 using four tilage methods： （i） deep tilage with plastic iflm mulching （CK）, （i） straw layer burial at 40 cm （S）, （ii） straw layer burial plus surface soil mulching with straw material （S＋S）, and （iv） plastic iflm mulching plus buried straw layer （P＋S）. Culturable microbes and predominant bacterial communities were studied; based on 16S rDNA, bacterial com-munity structure and abundance were characterized using denaturing gradient gel electrophoresis （DGGE） and polymerase chain reaction （PCR）. Results showed that P＋S was the most favorable for culturable bacteria, actinomyces and fungi and induced the most diverse genera of bacteria compared to other tilage methods. Soil temperature had signiifcant positive correlations with the number of bacteria, actinomyces and fungi （P〈0.01）. However, soil water was poorly correlated with any of the microbes. Salt content had a signiifcant negative correlation with the number of microbers, especialy for bacteria and fungi （P〈0.01）. DGGE analysis showed that the P＋S exhibited the highest diversity of bacteria with 20 visible bands folowed by S＋S, S and CK. Moreover, P＋S had the highest similarity （68%） of bacterial communities with CK. The major bacterial genera in al soil samples wereFirmicutes,Proteobacteria andActinobacteria. Given the considerable increase in microbial growth, the combined use of straw layer burial and plastic iflm mulching could be a practical option for aleviating salt stress effects on soil microbial community and thereby improving crop production in arid saline soils.