In a wood processing factory, the measured air concentration of birchen dust was 1. 26 ±0. 41 mg/m3, and the micronucleus frequency of peripheral blood lymphocytes in 83 workersexposed to wood dust was 1. 13 ...In a wood processing factory, the measured air concentration of birchen dust was 1. 26 ±0. 41 mg/m3, and the micronucleus frequency of peripheral blood lymphocytes in 83 workersexposed to wood dust was 1. 13 ± 2. 83%, which was significantly higher (P < 0.01 ) thanthat of control group (0. 51 ± 1. 41% ). The number of exposed workers with positive mi-cronucleus test was 9. 6 %, which was higher than that of control group (4. 5 % ), but thedifference was not significant (P >0. 05 ). The micronucleus test in mice treated with waterextracts of unsteamed and unbaked birchen dust showed that the micronucleus frequencies inall treated groups were significantly higher than that of contro group (P < 0. 01 ) and therewas also a doseresponse correlation (r = 0. 96, P < 0. 0005 ). The results of steamed andbaked birchen dust extracts were significantly lower than those of the unsteamed and unbakedones at the same doses (P< 0. 001 ). This suggests that when the birchen dust is steamed atthe temperature of 100℃ for 24h or baked at the temperature of 80℃, its inducing effect inmicronucleus test could be lowered展开更多
The micronucleus(MCN) test of Vicia faba root tip cell was used to screen the water quality of the Taihu Lake. The MCN ‰ and the pollution index ( PI ) were examined and the F test was carried out to ev...The micronucleus(MCN) test of Vicia faba root tip cell was used to screen the water quality of the Taihu Lake. The MCN ‰ and the pollution index ( PI ) were examined and the F test was carried out to evaluate the statistical difference in mean MCN ‰ among various samples. There were significant difference among thirty nine samples and there were six sites whose PI values are above 2. The authors suggest that the Taihu Lake has partly been polluted to different degrees and the whole water body can be divided into three types. Ames tests were then conducted to detect mutagenicity at five significant sites. The results showed that rivers flowing through urban area carried large amount of mutagenic pollutants into the lake and these pollutants contaminated the source of main waterworks.展开更多
Obejctive To assess the genotoxic effects of X ray radiation on human populations Methods The single cell gel electrophoresis (SCGE) and cytokinesis blocked micronucleus (CBMN) test were applied as biological dos...Obejctive To assess the genotoxic effects of X ray radiation on human populations Methods The single cell gel electrophoresis (SCGE) and cytokinesis blocked micronucleus (CBMN) test were applied as biological dosimeters to detect DNA damage and abnormalities in human peripheral lymphocytes of subpopulation exposed to X ray radiation The subjects were divided into four groups: 12 radiation patients; 13 intervention radiation therapy doctors; 32 radiation diagnostians; 28 controls Results The average comet lengths of the four groups were 128 17±4 49?μm, 88 09±5 39?μm, 72 68±2 57?μm and 32 87±0 57?μm, respectively The difference in average comet length between any two groups was highly significant ( P <0 01) The average micronucleated cell (MNC) rates (‰) of the four groups were 12 33±0 85, 9 75±1 02, 8 48±0 66 and 3 18±0 36, respectively The difference of MNC rates of Group 1 vs 3, 1 vs 4, 2 vs 4 and 3 vs 4 was highly significant ( P <0 01), and the difference of Group 1 vs 2 was significant ( P <0 05), but there was no difference of MNC rate in Group 2 vs 3 ( P >0 05) Conclusions This study showed that both the comet assay and the CBMN test could be used to monitor populations exposed to X ray radiation, but the comet assay seems to be more sensitive than the CBMN test展开更多
Objective:To assess the genotoxic potential of selected aquatic macrophytes:Ceratophyllum demersum L.(hornwort,family Ceratophyllaceae),Typha angustifolia L.(narrowleaf cattail,family Typhaceae),Stratiotes aloides L.(...Objective:To assess the genotoxic potential of selected aquatic macrophytes:Ceratophyllum demersum L.(hornwort,family Ceratophyllaceae),Typha angustifolia L.(narrowleaf cattail,family Typhaceae),Stratiotes aloides L.(water soldier,family Butomaceae),and Oenanthe aquatica(L.)Poir.(water dropwort,family Umbelliferae).Methods:For genotoxicity assessment,the mussel micronucleus test was applied.Micronucleus frequency was determined from the haemolymph of Unio pictorum L.(painter’s mussel).In parallel,total and hydrolisable tannin contents were determined.Results:All plant extracts elucidated significant mutagenic effect.Significant correlation was determined between tannin content and mutagenic capacity.Conclusions:The significant correlation between genotoxicity as expressed by micronucleus frequency and tannin content(both total and hydrolisable tannins)indicate that tannin is amongst the main compounds being responsible for the genotoxic potential.It might be suggested that genotoxic capacity of these plants elucidate a real ecological effect in the ecosystem.展开更多
文摘In a wood processing factory, the measured air concentration of birchen dust was 1. 26 ±0. 41 mg/m3, and the micronucleus frequency of peripheral blood lymphocytes in 83 workersexposed to wood dust was 1. 13 ± 2. 83%, which was significantly higher (P < 0.01 ) thanthat of control group (0. 51 ± 1. 41% ). The number of exposed workers with positive mi-cronucleus test was 9. 6 %, which was higher than that of control group (4. 5 % ), but thedifference was not significant (P >0. 05 ). The micronucleus test in mice treated with waterextracts of unsteamed and unbaked birchen dust showed that the micronucleus frequencies inall treated groups were significantly higher than that of contro group (P < 0. 01 ) and therewas also a doseresponse correlation (r = 0. 96, P < 0. 0005 ). The results of steamed andbaked birchen dust extracts were significantly lower than those of the unsteamed and unbakedones at the same doses (P< 0. 001 ). This suggests that when the birchen dust is steamed atthe temperature of 100℃ for 24h or baked at the temperature of 80℃, its inducing effect inmicronucleus test could be lowered
文摘The micronucleus(MCN) test of Vicia faba root tip cell was used to screen the water quality of the Taihu Lake. The MCN ‰ and the pollution index ( PI ) were examined and the F test was carried out to evaluate the statistical difference in mean MCN ‰ among various samples. There were significant difference among thirty nine samples and there were six sites whose PI values are above 2. The authors suggest that the Taihu Lake has partly been polluted to different degrees and the whole water body can be divided into three types. Ames tests were then conducted to detect mutagenicity at five significant sites. The results showed that rivers flowing through urban area carried large amount of mutagenic pollutants into the lake and these pollutants contaminated the source of main waterworks.
基金ThisresearchwassupportedbytheNaturalScienceFoundationofZhejiangProvince China (No 396 490 )
文摘Obejctive To assess the genotoxic effects of X ray radiation on human populations Methods The single cell gel electrophoresis (SCGE) and cytokinesis blocked micronucleus (CBMN) test were applied as biological dosimeters to detect DNA damage and abnormalities in human peripheral lymphocytes of subpopulation exposed to X ray radiation The subjects were divided into four groups: 12 radiation patients; 13 intervention radiation therapy doctors; 32 radiation diagnostians; 28 controls Results The average comet lengths of the four groups were 128 17±4 49?μm, 88 09±5 39?μm, 72 68±2 57?μm and 32 87±0 57?μm, respectively The difference in average comet length between any two groups was highly significant ( P <0 01) The average micronucleated cell (MNC) rates (‰) of the four groups were 12 33±0 85, 9 75±1 02, 8 48±0 66 and 3 18±0 36, respectively The difference of MNC rates of Group 1 vs 3, 1 vs 4, 2 vs 4 and 3 vs 4 was highly significant ( P <0 01), and the difference of Group 1 vs 2 was significant ( P <0 05), but there was no difference of MNC rate in Group 2 vs 3 ( P >0 05) Conclusions This study showed that both the comet assay and the CBMN test could be used to monitor populations exposed to X ray radiation, but the comet assay seems to be more sensitive than the CBMN test
基金Supported by Hungarian State and European Union(Grant No.TÁMOP-4.2.2.B-15/1/KONV-2015-0004).
文摘Objective:To assess the genotoxic potential of selected aquatic macrophytes:Ceratophyllum demersum L.(hornwort,family Ceratophyllaceae),Typha angustifolia L.(narrowleaf cattail,family Typhaceae),Stratiotes aloides L.(water soldier,family Butomaceae),and Oenanthe aquatica(L.)Poir.(water dropwort,family Umbelliferae).Methods:For genotoxicity assessment,the mussel micronucleus test was applied.Micronucleus frequency was determined from the haemolymph of Unio pictorum L.(painter’s mussel).In parallel,total and hydrolisable tannin contents were determined.Results:All plant extracts elucidated significant mutagenic effect.Significant correlation was determined between tannin content and mutagenic capacity.Conclusions:The significant correlation between genotoxicity as expressed by micronucleus frequency and tannin content(both total and hydrolisable tannins)indicate that tannin is amongst the main compounds being responsible for the genotoxic potential.It might be suggested that genotoxic capacity of these plants elucidate a real ecological effect in the ecosystem.
