Analysis of cellular behavior is significant for studying cell cycle and detecting anti-cancer drugs. It is a very difficult task for image processing to isolate individual cells in confocal microscopic images of non-...Analysis of cellular behavior is significant for studying cell cycle and detecting anti-cancer drugs. It is a very difficult task for image processing to isolate individual cells in confocal microscopic images of non-stained live cell cultures. Because these images do not have adequate textural variations. Manual cell segmentation requires massive labor and is a time consuming process. This paper describes an automated cell segmentation method for localizing the cells of Chinese hamster ovary cell culture. Several kinds of high-dimensional feature descriptors, K-means clustering method and Chan-Vese model-based level set are used to extract the cellular regions. The region extracted are used to classify phases in cell cycle. The segmentation results were experimentally assessed. As a result, the proposed method proved to be significant for cell isolation. In the evaluation experiments, we constructed a database of Chinese Hamster Ovary Cell’s microscopic images which includes various photographing environments under the guidance of a biologist.展开更多
针对原有万能工具显微镜的缺点,提出一种基于图像的万能工具显微镜改进方法.该方法采用电荷耦合器件CCD(Charge Coup led D ev ices)图像技术,可大大提高测量精度并降低操作人员的劳动强度.本文给出测量系统的结构和工作原理,建立了系...针对原有万能工具显微镜的缺点,提出一种基于图像的万能工具显微镜改进方法.该方法采用电荷耦合器件CCD(Charge Coup led D ev ices)图像技术,可大大提高测量精度并降低操作人员的劳动强度.本文给出测量系统的结构和工作原理,建立了系统数学模型,进行了系统误差分析并完成系统设计.该测量系统测量范围为200 mm×100 mm,其测量精度可达到1μm.展开更多
The discoveries of so-called quasicrystals have broken through the theoretic foundation set up by the classical crystallographic group theory since 1891 and proposed new topics for study of solid structures. Electron ...The discoveries of so-called quasicrystals have broken through the theoretic foundation set up by the classical crystallographic group theory since 1891 and proposed new topics for study of solid structures. Electron diffraction patterns (EDP' s) and high-resolution microscopic (HREM) images have proved invaluable tools of studying the structures of crystals. The recognition and determination of EDP's and HREM images of a real-structure play a key role for understanding the structure. This paper will introduce some new developments about crystallographic group theory and new image processing methods on EDP's and HREM images. Contrary to popular beliefs, the research shows that quasicrystals can be understood (perturbed) complex periodic structures.展开更多
在分析显微物镜成像原理的基础上,提出并实现了一种微操作机器人系统光轴方向深度测量方法,该方法通过显微物镜离焦光学传递函数(optical transfer function,OTF)建立物方离焦程度与显微图像模糊程度的关系,由此实现深度测量.由于考虑...在分析显微物镜成像原理的基础上,提出并实现了一种微操作机器人系统光轴方向深度测量方法,该方法通过显微物镜离焦光学传递函数(optical transfer function,OTF)建立物方离焦程度与显微图像模糊程度的关系,由此实现深度测量.由于考虑了透镜参数与衍射效应的影响,方法具有较高的测量精度和较好的线性度,同时利用条状物体快速辨识算法,对原始方法进行了简化,使深度测量可在线完成.进一步,将上述方法应用于实际微操作机器人系统,通过离焦状态双针互插实验验证了方法的有效性,同时拓展了微操作机器人系统的有效操作空间.展开更多
文摘Analysis of cellular behavior is significant for studying cell cycle and detecting anti-cancer drugs. It is a very difficult task for image processing to isolate individual cells in confocal microscopic images of non-stained live cell cultures. Because these images do not have adequate textural variations. Manual cell segmentation requires massive labor and is a time consuming process. This paper describes an automated cell segmentation method for localizing the cells of Chinese hamster ovary cell culture. Several kinds of high-dimensional feature descriptors, K-means clustering method and Chan-Vese model-based level set are used to extract the cellular regions. The region extracted are used to classify phases in cell cycle. The segmentation results were experimentally assessed. As a result, the proposed method proved to be significant for cell isolation. In the evaluation experiments, we constructed a database of Chinese Hamster Ovary Cell’s microscopic images which includes various photographing environments under the guidance of a biologist.
文摘针对原有万能工具显微镜的缺点,提出一种基于图像的万能工具显微镜改进方法.该方法采用电荷耦合器件CCD(Charge Coup led D ev ices)图像技术,可大大提高测量精度并降低操作人员的劳动强度.本文给出测量系统的结构和工作原理,建立了系统数学模型,进行了系统误差分析并完成系统设计.该测量系统测量范围为200 mm×100 mm,其测量精度可达到1μm.
文摘The discoveries of so-called quasicrystals have broken through the theoretic foundation set up by the classical crystallographic group theory since 1891 and proposed new topics for study of solid structures. Electron diffraction patterns (EDP' s) and high-resolution microscopic (HREM) images have proved invaluable tools of studying the structures of crystals. The recognition and determination of EDP's and HREM images of a real-structure play a key role for understanding the structure. This paper will introduce some new developments about crystallographic group theory and new image processing methods on EDP's and HREM images. Contrary to popular beliefs, the research shows that quasicrystals can be understood (perturbed) complex periodic structures.
文摘在分析显微物镜成像原理的基础上,提出并实现了一种微操作机器人系统光轴方向深度测量方法,该方法通过显微物镜离焦光学传递函数(optical transfer function,OTF)建立物方离焦程度与显微图像模糊程度的关系,由此实现深度测量.由于考虑了透镜参数与衍射效应的影响,方法具有较高的测量精度和较好的线性度,同时利用条状物体快速辨识算法,对原始方法进行了简化,使深度测量可在线完成.进一步,将上述方法应用于实际微操作机器人系统,通过离焦状态双针互插实验验证了方法的有效性,同时拓展了微操作机器人系统的有效操作空间.
