The biochemical methods usually were used to measure the content of cellular DNA. In these procedures the DNA must be separated from cells and purified before quantitative deter mination. But there are many difficulti...The biochemical methods usually were used to measure the content of cellular DNA. In these procedures the DNA must be separated from cells and purified before quantitative deter mination. But there are many difficulties in separation and purification of cellular DNA by those overloading procedures. In the last decade, microspectrophotometry appeared展开更多
DNA content of cells in effusion of 33 cases of mesothelial and adenocarcinomacells was measured by microspectrophotometer.The results showed that compared withmesothelial cells the DNA content of the suspected malign...DNA content of cells in effusion of 33 cases of mesothelial and adenocarcinomacells was measured by microspectrophotometer.The results showed that compared withmesothelial cells the DNA content of the suspected malignant cells was obviously increa-sed (P【0.01),the DNA content of both highly and poorly differentiate adenocarcinomacells was obviously increased and statistically significant difference existed between them(P【0.01);besides,aneuploidy could be found.It is objective,accurate and sensitive todetermine the character and differentiation degrees of adenocarcinoma cells in effusion byquantitaive analysis of DNA content with microspectrophotometer.展开更多
文摘The biochemical methods usually were used to measure the content of cellular DNA. In these procedures the DNA must be separated from cells and purified before quantitative deter mination. But there are many difficulties in separation and purification of cellular DNA by those overloading procedures. In the last decade, microspectrophotometry appeared
文摘DNA content of cells in effusion of 33 cases of mesothelial and adenocarcinomacells was measured by microspectrophotometer.The results showed that compared withmesothelial cells the DNA content of the suspected malignant cells was obviously increa-sed (P【0.01),the DNA content of both highly and poorly differentiate adenocarcinomacells was obviously increased and statistically significant difference existed between them(P【0.01);besides,aneuploidy could be found.It is objective,accurate and sensitive todetermine the character and differentiation degrees of adenocarcinoma cells in effusion byquantitaive analysis of DNA content with microspectrophotometer.
