We isolated a microsporidium from the silkworm, Bombyx mori, and classified it as Endoreticulatus sp. Zhenjiang based on morphological characteristics and phylogenetic analyses of ribosomal sequences. This microsporid...We isolated a microsporidium from the silkworm, Bombyx mori, and classified it as Endoreticulatus sp. Zhenjiang based on morphological characteristics and phylogenetic analyses of ribosomal sequences. This microsporidium causes silkworm pebrine, although its original host and mode of transmission are unknown. To better understand its distribution and transmission mode, it is essential to have species specific molecular markers. Towards this goal, we characterized the alpha tubulin gene from Endoreticulatus sp. Zhenjiang in this study. The full-length alpha-tubulin cDNA from Endoreticulatus sp. Zhenjiang was cloned and sequenced (GenBank ID: KJ784483) using the rapid amplification of cDNA ends (RACE) protocol. The alpha-tubulin cDNA is 1382 bp long with an open reading frame spanning 1320 bp and consisting of a short 20-bp 5’-untranslated region (5’-UTR) and a 42-bp 3’-UTR with a stop codon and a poly (A) tail. This alpha-tubulin cDNA encodes a deduced polypeptide with 439 amino acids, including a complete tubulin domain and a tubulin C domain. This protein has an estimated isoelectric point of 5.1 and a predicted molecular weight of 48.6 kDa.展开更多
The purpose of this study was to determine the frequency of emerging parasites in two groups of immunosuppressed patients, including individuals infected with the human immunodeficiency virus (AIDS) (HIV) or having ac...The purpose of this study was to determine the frequency of emerging parasites in two groups of immunosuppressed patients, including individuals infected with the human immunodeficiency virus (AIDS) (HIV) or having acute lymphoblastic leukemia (ALL) with or without diarrhea. Stool samples were collected from 96 HIV and 77 ALL patients from March 2010 through December 2011. Screening for opportunistic parasites was carried out by the coproparasitoscopic Faust method, Ziehl Neelsen staining, and Polymerase Chain Reaction (PCR). Results showed that 22.9% of HIV fecal samples were positive for emerging parasites, including Cryptosporidium spp. (7.3%), Microsporidium spp. (5.2%), Isospora belli (1.0%), Giardia intestinalis (2.6%), and Cyclospora spp. (7.3%). On the other hand, 32.5% of ALL fecal samples were positive for emerging parasites, including Cryptosporidium spp. (9.1%), Microsporidium spp. (19.5%), Isospora belli (1.3%), and Giardia intestinalis (2.6%). Our results highlighted the need for specific, efficient, and reliable diagnostic methods to identify the presence of emerging parasites in immunocompromised patients susceptible to different infectious diseases or neoplastic processes and avoid the consequences for the host as an increased disease rate, alterations in the clinical manifestation of the infection or even exacerbation of its course.展开更多
文摘We isolated a microsporidium from the silkworm, Bombyx mori, and classified it as Endoreticulatus sp. Zhenjiang based on morphological characteristics and phylogenetic analyses of ribosomal sequences. This microsporidium causes silkworm pebrine, although its original host and mode of transmission are unknown. To better understand its distribution and transmission mode, it is essential to have species specific molecular markers. Towards this goal, we characterized the alpha tubulin gene from Endoreticulatus sp. Zhenjiang in this study. The full-length alpha-tubulin cDNA from Endoreticulatus sp. Zhenjiang was cloned and sequenced (GenBank ID: KJ784483) using the rapid amplification of cDNA ends (RACE) protocol. The alpha-tubulin cDNA is 1382 bp long with an open reading frame spanning 1320 bp and consisting of a short 20-bp 5’-untranslated region (5’-UTR) and a 42-bp 3’-UTR with a stop codon and a poly (A) tail. This alpha-tubulin cDNA encodes a deduced polypeptide with 439 amino acids, including a complete tubulin domain and a tubulin C domain. This protein has an estimated isoelectric point of 5.1 and a predicted molecular weight of 48.6 kDa.
文摘The purpose of this study was to determine the frequency of emerging parasites in two groups of immunosuppressed patients, including individuals infected with the human immunodeficiency virus (AIDS) (HIV) or having acute lymphoblastic leukemia (ALL) with or without diarrhea. Stool samples were collected from 96 HIV and 77 ALL patients from March 2010 through December 2011. Screening for opportunistic parasites was carried out by the coproparasitoscopic Faust method, Ziehl Neelsen staining, and Polymerase Chain Reaction (PCR). Results showed that 22.9% of HIV fecal samples were positive for emerging parasites, including Cryptosporidium spp. (7.3%), Microsporidium spp. (5.2%), Isospora belli (1.0%), Giardia intestinalis (2.6%), and Cyclospora spp. (7.3%). On the other hand, 32.5% of ALL fecal samples were positive for emerging parasites, including Cryptosporidium spp. (9.1%), Microsporidium spp. (19.5%), Isospora belli (1.3%), and Giardia intestinalis (2.6%). Our results highlighted the need for specific, efficient, and reliable diagnostic methods to identify the presence of emerging parasites in immunocompromised patients susceptible to different infectious diseases or neoplastic processes and avoid the consequences for the host as an increased disease rate, alterations in the clinical manifestation of the infection or even exacerbation of its course.
