In order to understand the microtubule change of monocotyls stem-tip during mitosis, the arrangement, transformation of microtubule array and its relation with chromosome movement during mitosis were studied with free...In order to understand the microtubule change of monocotyls stem-tip during mitosis, the arrangement, transformation of microtubule array and its relation with chromosome movement during mitosis were studied with freezing microtome, indirect immunofluoreseenee, DAPI staining and fluorescence microscopy. The results showed that nucleolus was intact when the cortical microtubules formed; cortical microtubules were changed into phramoplast microtubules bands at mitosis prophase. When phramoplast microtubules came into being, nuclear membrane was ruptured and chromosome was arranged at the position of cell plate ; subsequently, phramoplast microtubules were changed into phragmoplast microtubules, phramoplast microtubules were shortening and microtubules on the sides of cell plate were increasing gradually, during this course sister ehromatid was separated by microtubules at cell plate and tract to the two poles, forming phragmoplast microtubules. Then the nucleolus of two daughter cells formed and separated in the end with the increase of cells numbers. Therefore, cell division orientation could be judged from the arrangement of cell microtubules in different periods in order to understand its growth status.展开更多
基金Supported by the National Natural Science Foundation of China(30060038)~~
文摘In order to understand the microtubule change of monocotyls stem-tip during mitosis, the arrangement, transformation of microtubule array and its relation with chromosome movement during mitosis were studied with freezing microtome, indirect immunofluoreseenee, DAPI staining and fluorescence microscopy. The results showed that nucleolus was intact when the cortical microtubules formed; cortical microtubules were changed into phramoplast microtubules bands at mitosis prophase. When phramoplast microtubules came into being, nuclear membrane was ruptured and chromosome was arranged at the position of cell plate ; subsequently, phramoplast microtubules were changed into phragmoplast microtubules, phramoplast microtubules were shortening and microtubules on the sides of cell plate were increasing gradually, during this course sister ehromatid was separated by microtubules at cell plate and tract to the two poles, forming phragmoplast microtubules. Then the nucleolus of two daughter cells formed and separated in the end with the increase of cells numbers. Therefore, cell division orientation could be judged from the arrangement of cell microtubules in different periods in order to understand its growth status.
