The distribution of motilin receptor in the amygdala of rats and its role in migrating myoelectric complex

Objective:To investigate the distribution of the motilin receptor in the amygdala of rats and its role in regulating the duodenal migrating myoelectric complex(MMC).Methods:The distribution of motilin receptor in the amygdala in adult SD rats was detected by immunohistochemistry methods,and the duodenal interdigestive MMC was recorded via the electrodes implanted in the duodenum and analyzed us- ing a multichannel recorder.Results:Motilin receptor was observed in the amygdala of rats.The great amount of motilin receptor was found in the medial amygdaloid nucleus,which was also abundant in the basolateral nucleus but less abundant in the basomedial amygdaloid nucleus,the central amygdaloid nucle- us and the lateral amygdaloid nucleus.The shortening of the duodenal MMC cycle duration and the in- crease of the amplitude and the frequency of phase $ were recorded after motilin receptors being bound with exogenous motilin in the amygdala.The effects could be completely blocked by the subdiaphragmatic vagotomy but not by the intravenous injections of atropine,phentolamine or propranolol.Anti-motilin serum could partially block these effects,and the destruction of the basolateral nucleus of the amygdala had no significant effects on the duodenal MMC.Conclusion:Motilin receptor is present in all the subnu- clei of the amygdala,with the greatest amount of motilin receptor present in the medial amygdaloid nucle- us.Microinjections of motilin in the amygdala can shorten the duodenal MMC cycle duration and increase the amplitude and the frequency of phaseⅢ.These effects might be accomplished via the amygdala-hy- pothalamus-brainstem-vagus pathway,indicating the important role of the amygdala motilin receptor in the duodenal MMC regulation.

Relationship between entero-hepatic bile acid circulation and interdigestive migrating myoelectrical activity in rats

AIM: To investigate the effects of entero-hepatic bile acid circulation on the inter-digestive migrating myoelectrical complex (MMC) in rats.METHODS: Thirty-two rats were divided into four groups.Three pairs of bipolar silver electrodes were chronically implanted in the antrum, duodenum and jejunum. Three groups of them were ligated around the upper part of common bile duct (CBD). The experiments were performed in conscious and fasting state. The gastrointestinal myoelectrical activity was recorded. Ursodeoxycholic acid (UDCA) and saline were then perfused into stomachs of two groups with CBD obstruction and the effects of them on the MMC were observed.RESULTS: A typical pattern of MMC was observed in normal fasting rats. MMC of antral and duodenal origin disappeared temporarily in earlier stage of CBD obstruction. While MMC of jejunum origin appeared.increased MMC cycle duration was seen after 4 d in rats with CBD obstruction. The MMC after CBD obstruction was characterized by an increased duration of phase Ⅱ-like activity and decreased duration of phase Ⅰ & Ⅲ activity.Perfusion into stomachs with UDCA resulted in a shorter MMC cycle duration and a longer duration of phase Ⅲ of duodenal origin compared to the normal group.CONCLUSION: Entero-hepatic bile acid circulation initiates inter-digestive MMC of duodenal origin.

Effects of PYY on the interdigestive migrating myoelectric complex in the small intestine in vivo and the neural and endocrinal mechanisms of the effects

Objective To investigate the effects of peptide YY (PYY) on the interdigestive migrating myoelectric complex (MMC) in the small intestine in vivo and explore the neural and endocrinal mechanisms of the effects. Methods Sprague-Dawley rats were supplied with a venous catheter and bipolar electrodes in the duodenum and jejunum for electromyography of stomach and small intestine in wake state. PYY,phentolamine,nitro-L-arginine (L-NNA,the inhibitor of nitric oxide synthase) and atropine were served with PYY respectively. The plasma motilin levels before and after the infusion of PYY were observed. Results At all the three recording points,PYY lengthened the circle length of MMC [from (591.90±128.98)s to (999.25±216.59)s,P<0.01] and lowered the frequency of phase Ⅲ [from (39.28±8.40) min-1 to (22.08±3.13) min-1,P<0.01],amplitude of phase Ⅲ [from (0.320±0.060)mV to (0.179±0.030)mV,P<0.01],and the portion of phase Ⅲ over the whole circle length [from (28.61±5.84)% to (15.43±5.16)% ,P<0.01]. Phentolamine had no influence on the role of PYY. Administered L-NNA combined with PYY,the percentage of phase Ⅲ increased [(42.09±8.67)%] compared with that of control (P<0.01) and compared with that of PYY administered alone (P<0.01) too. Atropine combined with PYY showed stronger depressing effects on MMC. No significant difference was found between the plasma motilin levels before and after the infusion of PYY. Conclusion PYY may inhibit the interdigestive intestine motility through the none-adrenergic none-cholinergic tract,while the α-receptor tract and circulating motilin are probably not involved in the depressing effect.

Effects of motilin in the hippocampus on the interdigestive migrating motor complex in rats

Objective:To explore the effects of motilin in the hippocampus on the interdigestive migrating motor complex (MMC) in rats. Methods: Adult SD rats of either sex were used; 0.5 μl motilin (0.74 mmol/L) was injected into the guide cannula which was stereotaxically implanted into the hippocampus previously. Then the MMC was recorded by a RM6240B multilead physiological recording system. Results: (1)MMC characteristics of normal rats’ duodenum: the frequency of phase Ⅲ was (18.1±0.4) bursts/min; the amplitude of phase Ⅲ was (260.5±42.3)μV; the duration of phase Ⅲ was (354.1± 21.6) s; MMC cycle duration was (690.2±58.7)s. (2) After motilin was injected into the hippocampus, the duodenal MMC cycle duration was decreased significantly. However, the amplitude of phase Ⅲ and the frequency of phase Ⅲ were increased. But there were no effects on the duration of phase Ⅲ. Frequency of phase Ⅲ percentage change was much more than amplitude of phase Ⅲ percentage change (57.2±2.8 vs 39.3±5.2). (3) Effects of motilin in the hippocampus on MMC were completely abolished by subdiaphragmal vagotomy. (4) Effects of motilin in the hippocampus on MMC were unaffected by intravenously injected atropine, phentolamine or propranolol. (5) The anti-motilin serum partly abolished the effects of motilin in the hippocampus on MMC. Conclusion: Motilin in the hippocampus has effects on the duodenal MMC cycle duration, the amplitude of phase Ⅲ and the frequency of phase Ⅲ. Motilin in the hippocampus plays an important role in duodenal MMC.

Role of the area postrema of medulla oblongata in the regulation of canine interdigestive migrating motor complex

OBJECTIVE: To determine the effect of the area postrema (AP) of the medulla oblongata on gastrointestinal interdigestive migrating motor complex (MMC) and the plasma motilin level. METHODS: Interdigestive MMC activities of the antrum and duodenum were recorded by strain gauge implanted on the serosa of 8 conscious dogs. A cannula was intubated in femoral vein for motilin injection. The plasma motilin concentration was measured by RIA. We observed: (1) normal interdigestive MMC activity and fluctuations in plasma motilin concentration; (2) the effects of electrically damaging the AP on MMC activity and plasma motilin level; (3) whether intravenous injection of motilin could induce phase III contractions after the AP was destroyed. RESULTS: (1) Typical interdigestive MMC with phase I, II, III, and IV was recorded in normal dogs. Phase III was concurrent with the peak of plasma motilin level. (2) In damaged AP dogs, antroduodenal interdigestive MMC contractions were suppressed; cyclic, phasic and migratory pattern of MMC was disrupted. Plasma motilin concentration was decreased. Intravenous injection of motilin could not induce phase III contractions. CONCLUSIONS: The area postrema might play an important role, which is mediated by motilin, on the regulation of interdigestive MMC.

十二指肠胃反流胃肠动力机制研究

背景:十二指肠胃反流(DGR)是一种常见的生理和病理现象,与许多疾病的发生有关。目前其发生机制尚不明确。目的:探讨DGR发生与胃窦十二指肠消化间期移行性复合运动(MMC)的关系。方法:对20名健康志愿者行24h同步胃内pH监测和胆汁监测,以及夜间长时胃窦十二指肠压力测定。结果:24h同步胃内pH监测和胆汁监测后,20名健康志愿者分为2组:DGR阴性组(D1组)(7名)和DGR阳性组(D2组)(13名)。D1组MMC周期数较D2组显著增加(P<0.05);D2组胃窦十二指肠协调收缩较D1组显著减少,十二指肠推进性蠕动减少(P均<0.05)。D1组十二指肠MMCⅢ相逆蠕动发生率显著低于D2组(P<0.05)。D2组发生MMCⅢ相逆蠕动前后10min,胃内pH值分别为1.72±0.61和3.70±0.72,差异有统计学意义(P<0.01)。夜间MMCⅡ相晚期碱反流和胆汁反流的发生率显著高于Ⅰ相、Ⅱ相早期和Ⅲ相(P均<0.05)。结论:DGR的发生与胃窦十二指肠MMC周期数、Ⅱ相晚期和Ⅲ相逆蠕动有关。

枳实对大鼠胃肠电活动影响的初步研究

目的 :研究枳实对大鼠胃肠移行性综合肌电 (MMC)的影响及其与P物质的关系。方法 :采用电生理学和免疫组织化学方法 ,观察枳实灌胃前后大鼠胃肠电活动及P物质含量的变化。结果 :浓缩枳实液可使大鼠胃肠MMC活动相时程与周期比值 (A/T)增大 ,胃肠肌间神经丛内P物质增多。结论 :枳实对大鼠胃肠MMC具有兴奋效应并可能与P物质有关。

大鼠海马区微量注射胃动素对十二指肠移动性复合肌电活动的影响

目的探讨海马微量注射胃动素对十二肠消化间期移行性复合肌电活动(migratingmyoelectricalcomplex,MMC)的影响及作用机制。方法采用中枢立体定位海马微量注射,多道生理记录仪监测十二指肠MMC的方法,观察海马微量注入胃动素(0.37nmol)对十二指肠MMC的影响。结果海马微量注入胃动素可以使十二指肠MMC时相明显变短,峰电振幅增加,峰电频率增快,但对峰电时相无明显影响;膈下迷走神经切断术可完全阻断海马胃动素对十二指肠MMC的影响;阿托品、酚妥拉明、普萘洛尔不能阻断海马胃动素对十二指肠MMC的影响;抗胃动素血清只能部分阻断海马胃动素对十二指肠MMC的影响。结论海马外源性胃动素可以使十二指肠MMC的时相、峰电振幅和峰电频率发生变化,这种作用可能是通过海马-下丘脑-脑干-迷走神经通路,由非肾上腺素能非胆碱能神经元作用于十二指肠平滑肌或者促进胃动素的释放而实现的,海马在十二指肠MMC的调节中发挥着重要作用。

Ghrelin对大鼠小肠转运及消化间期移行性复合肌电活动的作用及机制

目的探讨ghrelin对大鼠小肠转运和消化间期移行性复合肌电活动(MMC)的影响及作用机制。方法大鼠禁食24h,观察静脉给予不同剂量ghrelin对小肠转运的影响,及静脉给予ghrelin受体拮抗剂(D-Lys3)GHRP-6对ghrelin作用的影响。采用多道生理记录仪在大鼠清醒、禁食状态下监测消化间期MMC,观察静脉给予ghrelin对胃肠MMC的影响。分别给予阿托品、酚妥拉明、普萘洛尔、L-精氨酸及(D-Lys3)GHRP-6,探讨ghrelin对MMC的作用机制。结果静脉给予ghrelin剂量依赖性地促进小肠转运,此作用可被(D-Lys3)GHRP-6阻断。静脉给予ghrelin促进胃肠MMC。阿托品、L-精氨酸和(D-Lys3)GHRP-6不同程度地抑制ghrelin的促动力效应;酚妥拉明和普萘洛尔对ghrelin的促动力作用无显著影响。结论Ghrelin可促进胃肠运动,这可能是通过胆碱能通路起作用,与NO通路关系密切,ghrelin受体GHS-R参与其促动力作用。

甘草对大鼠胃动力功能影响的实验研究

目的:探讨甘草对大鼠胃动力的作用及其机制。方法:观察甘草组与空白组移行性综合肌电(MMC)的周期持续时间、Ⅲ相持续时间、Ⅲ相每分钟快波数(FM)和每簇的快波数(FC)的变化;采用免疫组织化学法结合显微图像定量分析扫描系统检测胃嗜铬细胞及其肌间神经丛中5羟色胺(5HT)、P物质(SP)、血管活性肠肽(VIP)的相对含量。结果:甘草组与空白组比较,MMCⅢ相FM和FC明显减少,MMC周期明显延长,Ⅲ相持续时间明显缩短,均有显著性差异(均P<0.05)。甘草组胃黏膜及肌间神经丛内5HT表达明显较空白组减少(P<0.05及P<0.01),胃窦黏膜无明显SP、VIP阳性免疫反应物表达,但胃肌间神经丛内SP含量明显减少(P<0.01)、VIP含量明显增加(P<0.05)。结论:甘草对大鼠胃动力有抑制作用,这种抑制作用可能与其引起5HT、SP、VIP分泌失调有关。

双歧三联活菌片对淤胆幼鼠移行性肌电复合波影响及其干预胆汁淤积的机制

目的观察益生菌制剂对急性肝内胆汁淤积幼鼠胃肠消化间期移行性肌电复合波（MMC）影响,探讨其干预胆汁淤积的可能机制。方法SD幼鼠96只随机分为健康对照组16只,中毒组、干预组各40只。各组随机选出8只在胃窦、十二指肠、空肠埋置3对银丝电极,余大鼠行假手术。术后7-10 d中毒及干预组1次灌服异硫氰酸萘酯（ANIT）（200 mg/kg）诱导急性肝内胆汁淤积;干预组于ANIT灌胃前2 d,灌服双歧三联活菌片4.2×10^8活菌/（kg.d）。观察各组灌服ANIT后48、96、144、192 h胆汁流量、血总胆红素（TB）、ALT及MMC变化。结果1.灌服ANIT后,干预组胆汁流量减少程度及血清中TB和ALT上升较中毒组轻,且恢复快。2.灌服ANIT后中毒及干预组MMC节律完全消失,代之以Ⅱ期样节律紊乱波;随后2组MMC逐渐恢复,但干预组MMC恢复时间（132.0±42.55）h明显短于中毒组（174.0±24.84）h（P〈0.05）;MMC节律恢复后,192 h时中毒组MMC持续时间较干预及健康对照组明显延长,其中主要是Ⅱ期持续时间延长。结论幼鼠急性肝内胆汁淤积时MMC周期延长,甚至节律性运动消失。双歧三联活菌片能促进肠道MMC节律性运动恢复,减轻肝脏损害,有助于改善胆汁淤积。

枳实对大鼠小肠电活动影响的下丘脑作用机制研究

目的观察枳实对在体大鼠小肠电活动的影响,探讨其作用与下丘脑内脑肠肽胆囊收缩素(CCK)、生长抑素(SS)的相关性。方法采用消化道腔外电极记录法观察枳实组与生理盐水组移行性综合肌电(MMC)周期时程、活动期/周期比例、活动期每分钟快波数的变化；采用免疫组织化学法结合显微图像定量分析扫描系统检测下丘脑背内侧核(DMH)、腹内侧核(VMH)、下丘脑外侧区(LHA)CCK的相对含量,以及下丘脑VMH、LHA、室旁核(PVN)SS的相对含量。结果枳实组与生理盐水组比较MMC周期缩短、活动期/周期比例增大、活动期每分钟快波数增加,差异有显著性；与生理盐水组及空白对照组比较枳实组DMH、VMH、LHA的CCK阳性神经元表达降低；VMH、LHA、PVN的SS阳性神经元表达增高,与生理盐水组及空白对照组比较差异有显著性。结论枳实对大鼠小肠电活动有兴奋作用,这种兴奋作用可能与下丘脑内的CCK、SS有关。

甘草对大鼠小肠动力功能影响的实验研究

目的初步探讨甘草对大鼠小肠动力的作用,及其作用与胃肠激素的相关性。方法观察甘草组与空白组移行性综肌电(MMC)周期持续时间、Ⅲ相持续时间、Ⅲ相每分钟快波数(FM)和每簇的快波数(FC)的变化;采用免疫组织化学法结合显微图像定量分析扫描系统检测十二指肠、空肠嗜铬细胞及其肌间神经丛中5羟色胺(5HT)、P物质(SP)、血管活性肠肽(VIP)的相对含量。结果①甘草组与空白组比较MMCⅢ相FM和FC明显减少,MMC周期明显延长,Ⅲ相持续时间明显缩短,统计有显著性差异(P<0.05)。②甘草组十二指肠、空肠粘膜及肌间神经丛内5HT表达明显较空白组减少,比较有显著性差异,小肠粘膜无明显SP、VIP阳性免疫反映物表达,但小肠肌间神经丛内SP含量明显减少、VIP含量明显增加,组间比较有显著性差异(P<0.05,P<0.01)。结论甘草对大鼠小肠动力有抑制作用,这种抑制作用与5HT、SP、VIP分泌失调密切相关。

红霉素对狗胃肠道电活动的影响

以移行性综合肌电(MMC)作指标,研究红霉素(EM)消化道副作用与平滑肌电活动的关系。结果发现,在狗消化间期,静脉注射EM50～400μg/kg,在3～7min内即诱发剂量依赖性早发MMCⅢ相。此早发Ⅲ相起于胃、十二指肠,向小肠尾端移行,其锋电发生率、Ⅲ相持续时间和移行速度等均与自发Ⅲ相类似。胃、十二指肠内分别注射EM500μg/kg,经24.5±7.5min和23.7±2.2min才诱发移行性Ⅲ相。双侧膈上迷走神经切除后,EM仍诱发早发Ⅲ相,但其移行速度减慢(p<0.01),阿托品能阻断EM诱发Ⅲ相的发生。EM在Ⅲ相诱发后的70～250min甚至更长时间内仍使MMC周期和Ⅲ相起始紊乱,甚至完全破坏MMG,代之以超速扩布锋电簇活动。EM不影响进食后狗的胃肠电活动。因此,EM消化道反应的发生可能与其被吸收进血液后通过内在胆碱能神经启动早发Ⅲ相及其后较长后效应有关。

禁食大鼠胃肠肌电活动和胃动素与熊去氧胆酸的关系

目的 研究大鼠禁食状态下不同起源的胃肠移行性复合肌电活动 (MMC)的特征异同以及胃动素 (MTL)和熊去氧胆酸 (UDCA)对不同起源MMC的作用。方法 2 4只大鼠被随机分为两组。在胃窦、十二指肠及空肠慢性埋置银丝电极。术后 7d在大鼠清醒、禁食状态下实验。第一组静脉注入合成猪MTL ,第二组给予UDCA灌胃。记录给药前 1h至给药后 2h胃肠肌电活动。结果 实验共观察到 6 8个正常的MMC波形 ,其中 33%起源于胃窦 ,6 7%起源于十二指肠。起源于胃窦的MMC波的周期和Ⅲ期的时程明显长于十二指肠起源的MMC波的相应参数。静脉注射合成猪MTL可引发起源于胃窦的不成熟Ⅲ相波 ,UDCA灌胃可使起源于十二指肠的MMC波周期缩短 ,Ⅲ期时程延长。结论 禁食大鼠MMC波可起源于胃窦和十二指肠 ,不同起源MMC波形特征不同。MTL和UDCA可分别影响禁食状态下不同起源的胃肠肌电活动。

清热化滞颗粒对积食大鼠十二指肠肌电活动的影响

目的探讨清热化滞颗粒对十二指肠肌电活动的影响。方法采用电生理学方法,观察空白组、造模组及化滞组大鼠十二指肠肌电活动的变化。结果造模组大鼠十二指肠MMC周期与空白组相比明显延长,峰值振幅明显降低;化滞组大鼠与造模组相比MMC周期明显缩短,峰值振幅明显升高。结论清热化滞颗粒可能通过增加十二指肠肌电活动而起到化滞清热的作用。

大鼠杏仁核胃动素受体分布及其作用研究

目的探讨大鼠杏仁核胃动素受体分布及杏仁核基底外侧核胃动素受体与外源性胃动素结合或者毁损后对十二指肠消化间期移行性复合肌电活动(MMC)的影响及其作用机制。方法采用免疫组织化学、中枢立体定位杏仁核微量注射、杏仁核毁损、多道生理记录仪监测十二指肠MMC的方法,探讨大鼠杏仁核胃动素受体的作用。结果杏仁核各亚核团都有胃动素受体分布,以杏仁内侧核分布最多;杏仁基底外侧核也有较多分布,后部多于前部;分布较少的核团是杏仁基底内侧核、杏仁中央核、杏仁基底外侧核;杏仁核基底外侧核胃动素受体与外源性胃动素结合后可以使十二指肠MMC时相变短,峰电振幅增加,峰电频率增快;膈下迷走神经切断术可完全阻断这种效应;阿托品、酚妥拉明、普萘洛尔不能阻断这种效应;抗胃动素血清只能部分阻断这种效应;杏仁基底外侧核核团毁损对十二指肠MMC影响不明显。结论杏仁核各亚核团都有胃动素受体分布,杏仁内侧核是胃动素受体分布最多的部位。杏仁核微量注射胃动素可以使十二指肠MMC的时相、峰电振幅和峰电频率发生变化,这种作用可能是通过杏仁核-下丘脑-脑干-迷走神经通路实现的,提示杏仁核胃动素受体在对十二指肠MMC的中枢性调节中发挥着重要作用。

Ghrelin对CKD大鼠十二指肠消化间期移行性复合运动和Bcl-2/Bax表达的影响

目的观察ghrelin对慢性肾功能不全(CKD)大鼠十二指肠消化间期移行性复合运动变化和Bcl-2/Bax表达的影响。方法 SD大鼠18只,体重(180±20)g,雌雄不拘,随机分为对照组(CKD+生理盐水)、ghrelin 1组(CKD+ghrelin 10μg/kg)和ghrelin 2组(CKD+ghrelin 50μg/kg),每组6只。采用5/6肾切除法建立CKD大鼠模型。模型建立成功后分别采用多道生理记录仪和免疫组织化学方法观察十二指肠消化间期移行性复合运动变化和凋亡相关基因Bcl-2/Bax的表达。结果 CKD大鼠普遍存在十二指肠运动功能障碍;ghrelin可以明显增加CKD大鼠十二指肠消化间期移行性复合运动出现的频率,包括呈剂量依赖性增加Ⅲ相出现的时间、频率和振幅,各参数在对照组、ghrelin 1组和ghrelin 2组分别为:Ⅲ相出现的时间[(0.81±0.12)minvs.(0.84±0.13)minvs.(0.99±0.17)min];频率[(22.68±1.00)次/minvs.(23.55±1.08)次/minvs.(29.31±1.51)次/min];振幅[(249.71±13.98)μVvs.(258.49±13.12)μVvs.(318.85±20.10)μV](P<0.05)。同时ghrelin可以明显减少Bcl-2和Bax的表达,其中以Bax的减少更明显(P<0.05),Bcl-2/Bax比值增加(P<0.05)。结论 ghrelin可以明显改善CKD大鼠十二指肠的运动功能,有可能成为一种新型胃肠动力药物。

实验性急性肝内胆汁淤积幼年大鼠胃肠消化间期移行性肌电复合波的变化

目的:探讨异硫氰酸萘酯(ANIT)所致刚断乳大鼠急性肝内胆汁淤积胃肠消化间期移行性肌电复合波(MMC)的变化.?方法:?56只刚断乳SD大鼠被随机分为正常对照组(n?=?16)、中毒组(n?=?40).?两组分别随机取8只在胃窦、十二指肠、空肠分别慢性埋置三对银丝电极;?其余大鼠同时行假手术.?术后7-10?d,?埋置电极组大鼠均在清醒、空腹、自由活动状态下记录胃肠道MMC.?中毒组按200?mg/kg一次性灌服ANIT诱发大鼠急性肝内胆汁淤积病变,?观察灌服ANIT后中毒组在48,?96,?144,?192?h胆汁流量、血中TB和ALT值及胃肠MMC的变化.?结果:中毒组灌服ANIT后,?48?h时胆汁流量中断,?血清TB和ALT明显升高;?48?h后其胆汁流量逐渐增加,?血清TB和ALT逐渐下降,?于192?h基本恢复.?在ANIT灌胃前,?埋置电极的所有大鼠在清醒、禁食状态下均记录到典型的MMC节律性电活动,?正常对照组与中毒组之间差异无显著性意义;?中毒组灌服ANIT后,?MMC节律完全消失,?代之以Ⅱ期样节律紊乱波;?144?h后,?中毒组MMC节律运动开始恢复;?192?h时中毒组MMC均为节律运动,?但中毒组MMC持续时间(911.67±140.47?s)较正常对照组(682.87±77.39?s)明显延长,?其中主要是Ⅱ期持续时间延长(414.12±69.21?vs?150.28±35.45?s),?而Ⅲ期持续时间略缩短(121.21±27.38?s?vs?170.27±38.98?s)?,?差异有显著意义(P<0.05).结论:?急性肝内胆汁淤积时胃肠MMC表现MMC节律短暂消失或MMC周期延长;?其部分原因可能与消化间期胆汁流量减少有关.

芍药甘草汤对顺铂所致的大鼠小肠电活动改变的影响

在大鼠小肠浆膜面埋植铂丝双极电极，以移行性综合肌电（ＭＭＣ）为指标，观察芍药甘草汤对顺铂所引致的大鼠小肠电活动改变的影响。静脉注入顺铂后，ＭＭＣ活动改变，其周期持续时间与正常ＭＭＣ周期持续时间相比明显缩短（Ｐ＜０．０５），而Ⅲ相持续时间与正常Ⅲ相持续时间比较显著延长（Ｐ＜０．０１）。口服芍药甘草汤后，静脉注入顺铂不再引致ＭＭＣ活动的改变。结果提示：芍药甘草汤对顺铂所引致的ＭＭＣ改变有明显的纠正作用，这可能是其制止顺铂所引致的大鼠腹泻的原因之一。