In order to reduce the use of thickening agent in yogurt and increase the viscosity of fermented milk,we screened two probiotics from breast milk as co-cultured with commercial fermenting bacteria in the previous stud...In order to reduce the use of thickening agent in yogurt and increase the viscosity of fermented milk,we screened two probiotics from breast milk as co-cultured with commercial fermenting bacteria in the previous study,and found that the two strains(i.e.Limosilactobacillus fermentum MWLf-4 and Lactipiantibacillus plantarum MWLp-12)had a great ability to increase the viscosity of fermented milk.After that,in this paper we observed the extracellular polysaccharides of single strain fermented milk using inverted fluorescence microscope.Furthermore,the complete gene sequences and safety evaluation including antibiotic sensitivity test,hemolysis test and determination of amine in vitro of Limosilactobacillus fermentum MWLf-4 and Lactipiantibacillus plantarum MWLp-12 were carried out.As a conclusion,the two strains possess a better ability to produce extracellular polysaccharide,and they are potentially safe strains.It is worth noting that the genes that produce exopolysaccharides of tagE and glmU are found in Limosilactobacillus fermentum MWLf-4,and cps gene cluster and galE are found in Lactipiantibacillus plantarum MWLp-12.Therefore,this study provides a reliable theoretical basis for further elaborating the relationship between genes and functionality.展开更多
基金support was kindly supplied by National Key R&D Program of China(2021YFD2100700)。
文摘In order to reduce the use of thickening agent in yogurt and increase the viscosity of fermented milk,we screened two probiotics from breast milk as co-cultured with commercial fermenting bacteria in the previous study,and found that the two strains(i.e.Limosilactobacillus fermentum MWLf-4 and Lactipiantibacillus plantarum MWLp-12)had a great ability to increase the viscosity of fermented milk.After that,in this paper we observed the extracellular polysaccharides of single strain fermented milk using inverted fluorescence microscope.Furthermore,the complete gene sequences and safety evaluation including antibiotic sensitivity test,hemolysis test and determination of amine in vitro of Limosilactobacillus fermentum MWLf-4 and Lactipiantibacillus plantarum MWLp-12 were carried out.As a conclusion,the two strains possess a better ability to produce extracellular polysaccharide,and they are potentially safe strains.It is worth noting that the genes that produce exopolysaccharides of tagE and glmU are found in Limosilactobacillus fermentum MWLf-4,and cps gene cluster and galE are found in Lactipiantibacillus plantarum MWLp-12.Therefore,this study provides a reliable theoretical basis for further elaborating the relationship between genes and functionality.
