OBJECTIVE To observe the anti-aging effects of SOD mimic AEOL^(-1)0150 in antisenescence accelerated mouse resistant 1(SAMR1)strain.METHODS The lifespan of SAMR1 mice were observed by subcutaneous injection AEOL^(-1)0...OBJECTIVE To observe the anti-aging effects of SOD mimic AEOL^(-1)0150 in antisenescence accelerated mouse resistant 1(SAMR1)strain.METHODS The lifespan of SAMR1 mice were observed by subcutaneous injection AEOL^(-1)0150 2 mg·kg-1once a week.Morris water maze,new object recognition,nesting and forced swimming were used to observe the behavioral changes of animals.Lymphocyte subgroups and ROS were measured by Flow cytometry.The cytokines levels were determined by Luminex method.The number of DCX+neurons in brain tissue was observed by immunofluorescence.RESULTS The results showed that AEOL^(-1)0150 could prolong the mean lifespan of SAMR1 mice,but it had no obvious effect on maximal lifespan.What′s more,AEOL^(-1)0150 could significantly improve the spatial learning memory of aged mice,but it could not increase the number of DCX+neurons in the hypothalamic MBH and hippocampal DG regions.Then,we observed the effects of AEOL^(-1)0150 on peripheral blood lymphocyte subgroups and cytokines.We found that AEOL^(-1)0150significantly modulated the lymphocyte subgroups and cytokine release.Especially,AEOL^(-1)0150 can dose-dependently inhibit plasma levels of SASP related inflammatory cytokines TNF-αand IL^(-1)7.CONCLUSION The results indicate that AEOL^(-1)0150 has anti-aging effects,and the effects are closely related to modulating immunity and inhibiting SASP production.展开更多
Objective: To explore the biological role of ptotocatechualdehyde-thiosemicarbazone-copper(Ⅱ)complex (PTC), its scavenging O2- free radical mechanism and to develop a new drug with high effective and low-toxicity. On...Objective: To explore the biological role of ptotocatechualdehyde-thiosemicarbazone-copper(Ⅱ)complex (PTC), its scavenging O2- free radical mechanism and to develop a new drug with high effective and low-toxicity. One of the essential components of this metal complex is an extract from a traditional Chinese medicinal herb. Methods:①Xathine- Xathine oxidase-luminol chemiluminescence (CL)was applied to detect SOD-like activity of PTC. ②Effects of SOD and its mimic (PTC) on the free radical-mediated change of blood viscosity and zymosan-activated leukocytic chemiluminescence, as well as the prptectove effects of PTC on oxiditive damage after burn were observed. Results: ①PTC has a significantly high SOD-like activity with an [ID]50 of 150 μg/L (SOD-300 μg/L). ②PTC can significantly inhibit zymosan-activated leukocytic chemiluminescence.③PTC can lower phagocyte-dependent increase in blood viscosity and inhibit the lipid peroxidation in blood. ④PTC can alleviate secondary oxygen free radical injury after burn. Conclusion:PTC is a significantly effective O2- free radical scanvenger. It can be applied to treat diseases resulting from abnormality of active oxygen free radical reaction in the body.展开更多
Three complexes of Mn（Ⅱ）with L-amino acid[L-Isoleucine（L-Ile）,L-Methionine（L-Met）,L-Tryptophan（L-Trp）]were synthesized and characterized by elemental analysis,infrared spectra and gravithem metrical analyses....Three complexes of Mn（Ⅱ）with L-amino acid[L-Isoleucine（L-Ile）,L-Methionine（L-Met）,L-Tryptophan（L-Trp）]were synthesized and characterized by elemental analysis,infrared spectra and gravithem metrical analyses.The catalytic activity of the three complexes for disproportionation of O2-was determined by NBT method.The result shows that the complexes have a high SOD-like activity,and the KQ values of complexes 1,2 and 3 are 0.78×107mol-1·L·s-1,1.28×107mol-1,and 1.50×107mol-1L·s-1,respectively.展开更多
Inorganic solids with enzyme-like activity are promising to overcome many restrictions of native enzymes in application.Especially attractive are nanoparticles with superoxide dismutase(SOD)activity,due to their abili...Inorganic solids with enzyme-like activity are promising to overcome many restrictions of native enzymes in application.Especially attractive are nanoparticles with superoxide dismutase(SOD)activity,due to their ability to reduce the damaging properties of reactive oxygen species within cells and organism.This review discusses the necessary requirements for nanoparticles to have SOD activity and reveals a close relationship between catalysis on prebiotic earth and the recent SOD mimics.This review also aims to highlight the progress in the development of SOD mimicking nanoparticles.We give a broad overview of nanoparticles with SOD activity,based on their material make-up,to underline their increasing diversity.展开更多
基金supported by Chinese Scientific and Technological Major Special Project(2014ZX09J13103-01B-003 and 2014ZX09J15104002)
文摘OBJECTIVE To observe the anti-aging effects of SOD mimic AEOL^(-1)0150 in antisenescence accelerated mouse resistant 1(SAMR1)strain.METHODS The lifespan of SAMR1 mice were observed by subcutaneous injection AEOL^(-1)0150 2 mg·kg-1once a week.Morris water maze,new object recognition,nesting and forced swimming were used to observe the behavioral changes of animals.Lymphocyte subgroups and ROS were measured by Flow cytometry.The cytokines levels were determined by Luminex method.The number of DCX+neurons in brain tissue was observed by immunofluorescence.RESULTS The results showed that AEOL^(-1)0150 could prolong the mean lifespan of SAMR1 mice,but it had no obvious effect on maximal lifespan.What′s more,AEOL^(-1)0150 could significantly improve the spatial learning memory of aged mice,but it could not increase the number of DCX+neurons in the hypothalamic MBH and hippocampal DG regions.Then,we observed the effects of AEOL^(-1)0150 on peripheral blood lymphocyte subgroups and cytokines.We found that AEOL^(-1)0150significantly modulated the lymphocyte subgroups and cytokine release.Especially,AEOL^(-1)0150 can dose-dependently inhibit plasma levels of SASP related inflammatory cytokines TNF-αand IL^(-1)7.CONCLUSION The results indicate that AEOL^(-1)0150 has anti-aging effects,and the effects are closely related to modulating immunity and inhibiting SASP production.
文摘Objective: To explore the biological role of ptotocatechualdehyde-thiosemicarbazone-copper(Ⅱ)complex (PTC), its scavenging O2- free radical mechanism and to develop a new drug with high effective and low-toxicity. One of the essential components of this metal complex is an extract from a traditional Chinese medicinal herb. Methods:①Xathine- Xathine oxidase-luminol chemiluminescence (CL)was applied to detect SOD-like activity of PTC. ②Effects of SOD and its mimic (PTC) on the free radical-mediated change of blood viscosity and zymosan-activated leukocytic chemiluminescence, as well as the prptectove effects of PTC on oxiditive damage after burn were observed. Results: ①PTC has a significantly high SOD-like activity with an [ID]50 of 150 μg/L (SOD-300 μg/L). ②PTC can significantly inhibit zymosan-activated leukocytic chemiluminescence.③PTC can lower phagocyte-dependent increase in blood viscosity and inhibit the lipid peroxidation in blood. ④PTC can alleviate secondary oxygen free radical injury after burn. Conclusion:PTC is a significantly effective O2- free radical scanvenger. It can be applied to treat diseases resulting from abnormality of active oxygen free radical reaction in the body.
文摘Three complexes of Mn（Ⅱ）with L-amino acid[L-Isoleucine（L-Ile）,L-Methionine（L-Met）,L-Tryptophan（L-Trp）]were synthesized and characterized by elemental analysis,infrared spectra and gravithem metrical analyses.The catalytic activity of the three complexes for disproportionation of O2-was determined by NBT method.The result shows that the complexes have a high SOD-like activity,and the KQ values of complexes 1,2 and 3 are 0.78×107mol-1·L·s-1,1.28×107mol-1,and 1.50×107mol-1L·s-1,respectively.
文摘Inorganic solids with enzyme-like activity are promising to overcome many restrictions of native enzymes in application.Especially attractive are nanoparticles with superoxide dismutase(SOD)activity,due to their ability to reduce the damaging properties of reactive oxygen species within cells and organism.This review discusses the necessary requirements for nanoparticles to have SOD activity and reveals a close relationship between catalysis on prebiotic earth and the recent SOD mimics.This review also aims to highlight the progress in the development of SOD mimicking nanoparticles.We give a broad overview of nanoparticles with SOD activity,based on their material make-up,to underline their increasing diversity.
