Minipump is widely used in microfluidics system, active cooling system, etc. But building a high efficiency minipump is still a challenging problem. In this paper, a systematic method was developed to design, characte...Minipump is widely used in microfluidics system, active cooling system, etc. But building a high efficiency minipump is still a challenging problem. In this paper, a systematic method was developed to design, characterize and optimize a particular mechanical minipump. The optimization work was conducted to cope with the conflict between pressure head and hydraulic efficiency by an improved back-propagation neural network （BPNN） with the non-dominated sorting genetic algorithm-II （NSGA-II）. The improved BPNN was utilized to predicate hydraulic performance and, moreover, was modified to improve the prediction accuracy. The NSGA-II was processed for minipump multi-objective optimization which is dominated by four impeller dimensions. During hydraulic optimization, the processing feasibility was also taken into consideration. Experiments were conducted to validate the above optimization methods. It was proved that the optimized minipump was improved by about 24 % in pressure head and 4.75 % in hydraulic efficiency compared to the original designed prototype. Meanwhile, the sensitivity test was used to analyze the influence of the four impeller dimensions. It was found that the blade outlet angle β2 and the impeller inlet diameter Do significantly influence the pressure head H and the hydraulic efficiency η, respec- tively. Detailed internal flow fields showed that the optimum model can relieve the impeller wake and improve both the pressure distribution and flow orientation.展开更多
Summary: To construct an immortalized rat astrocyte strain genetically modified by rat preprogalanin gene (IAST/GAL) and detect its galanin (GAL) expression and secretion, a cDNA fragment of rat GAL in plasmid of pBS...Summary: To construct an immortalized rat astrocyte strain genetically modified by rat preprogalanin gene (IAST/GAL) and detect its galanin (GAL) expression and secretion, a cDNA fragment of rat GAL in plasmid of pBS KS(+)-GAL was inserted into eukaryotic expression vector pcDNA3.1(+) by DNA recombinant technology, then the restriction enzyme digestion and DNA sequencing were carried out to evaluate the recombinant. The pcDNA3.1(+)-GAL and pcDNA3.1(+) construct were transfected into immortalized rat astrocyte strain (IAST) by lipofectamine and the population of cells which stably integrated the construct was selected with 600 μg/mL G418. Individual clones were screened and expanded into clonal cell strains. Detection of Neo gene was used to validate the success of the transfection. Immunocytochemical staining, RT-PCR and radioimmunoassay were used to detect the expression and secretion level of GAL. The recombinant had been successfully constructed by restriction enzyme digestion and DNA sequencing. Detection of Neo gene showed that the pcDNA3.1(+)-GAL and pcDNA3.1(+) have been successfully transfected into IAST. After selection by using G418, IAST/GAL and IAST/Neo cell strains were obtained. IAST/GAL, IAST/Neo and IAST were immunostained positively for GAL, but the GAL average optical density of IAST/GAL was significantly higher than that of IAST/Neo and IAST (P< 0.01). The level of GAL mRNA expression and the supernatant concentration of GAL in cultured IAST/GAL were significantly higher than those of IAST and IAST/Neo (P<0.01), but no significant differences were found between the IAST and IAST/Neo (P>0.05). It was concluded that IAST/GAL strain was constructed successfully and it might provide a basis for the further study of pain therapy.展开更多
文摘Minipump is widely used in microfluidics system, active cooling system, etc. But building a high efficiency minipump is still a challenging problem. In this paper, a systematic method was developed to design, characterize and optimize a particular mechanical minipump. The optimization work was conducted to cope with the conflict between pressure head and hydraulic efficiency by an improved back-propagation neural network （BPNN） with the non-dominated sorting genetic algorithm-II （NSGA-II）. The improved BPNN was utilized to predicate hydraulic performance and, moreover, was modified to improve the prediction accuracy. The NSGA-II was processed for minipump multi-objective optimization which is dominated by four impeller dimensions. During hydraulic optimization, the processing feasibility was also taken into consideration. Experiments were conducted to validate the above optimization methods. It was proved that the optimized minipump was improved by about 24 % in pressure head and 4.75 % in hydraulic efficiency compared to the original designed prototype. Meanwhile, the sensitivity test was used to analyze the influence of the four impeller dimensions. It was found that the blade outlet angle β2 and the impeller inlet diameter Do significantly influence the pressure head H and the hydraulic efficiency η, respec- tively. Detailed internal flow fields showed that the optimum model can relieve the impeller wake and improve both the pressure distribution and flow orientation.
基金This project was supported by a grant from the National Natural Science Foundation of China (No. 30170905).
文摘Summary: To construct an immortalized rat astrocyte strain genetically modified by rat preprogalanin gene (IAST/GAL) and detect its galanin (GAL) expression and secretion, a cDNA fragment of rat GAL in plasmid of pBS KS(+)-GAL was inserted into eukaryotic expression vector pcDNA3.1(+) by DNA recombinant technology, then the restriction enzyme digestion and DNA sequencing were carried out to evaluate the recombinant. The pcDNA3.1(+)-GAL and pcDNA3.1(+) construct were transfected into immortalized rat astrocyte strain (IAST) by lipofectamine and the population of cells which stably integrated the construct was selected with 600 μg/mL G418. Individual clones were screened and expanded into clonal cell strains. Detection of Neo gene was used to validate the success of the transfection. Immunocytochemical staining, RT-PCR and radioimmunoassay were used to detect the expression and secretion level of GAL. The recombinant had been successfully constructed by restriction enzyme digestion and DNA sequencing. Detection of Neo gene showed that the pcDNA3.1(+)-GAL and pcDNA3.1(+) have been successfully transfected into IAST. After selection by using G418, IAST/GAL and IAST/Neo cell strains were obtained. IAST/GAL, IAST/Neo and IAST were immunostained positively for GAL, but the GAL average optical density of IAST/GAL was significantly higher than that of IAST/Neo and IAST (P< 0.01). The level of GAL mRNA expression and the supernatant concentration of GAL in cultured IAST/GAL were significantly higher than those of IAST and IAST/Neo (P<0.01), but no significant differences were found between the IAST and IAST/Neo (P>0.05). It was concluded that IAST/GAL strain was constructed successfully and it might provide a basis for the further study of pain therapy.
