Objective: To study the possibility of mismatch repair gene hMSH2 as a marker to predict the occurrence of gastric carcinoma. Methods: Immunohistochemical method was used to detect hMSH2 protein expressions of gastric...Objective: To study the possibility of mismatch repair gene hMSH2 as a marker to predict the occurrence of gastric carcinoma. Methods: Immunohistochemical method was used to detect hMSH2 protein expressions of gastric carci- nomas (carcinoma group) and their surrounding epithelia (surrounding epithelium group) in patients and the epithelia (normal epithelium group) in persons without carcinoma. Results: The positive rates of hMSH2 protein expression in nucleus of carcinoma, surrounding epithelium and normal epithelium groups were 44.94% (71/158), 28.48% (45/158) and 11.76% (4/34), respectively (P=0.000); the positive rates of hMSH2 protein expression in plasma of the 3 groups were 37.97% (60/158), 27.85% (44/158) and 23.53% (8/34), respectively (P=0.084); the positive rates of hMSH2 protein expression in both nucleus and plasma of the 3 groups were 20.89% (33/158), 17.72% (28/158) and 2.94% (1/34), respectively (P=0.045). Although the difference of positive rates between carcinoma and surrounding epithelium groups was not significant (P=0.476), both of them were higher than that of normal epithelium group (P=0.018). Conclusion: Our findings show that the detection of hMSH2 protein expression in gastric epithelia may help to predict and diagnose gastric carcinoma.展开更多
目的:探讨人类错配修复基因hM LH 1、hM SH 2在肺癌组织中的表达及意义。方法:运用免疫组织化学S-P法对56例肺癌组织中hM LH 1、hM SH 2的表达进行检测。结果:56例肺癌组织中hM LH 1的阳性表达率为35%,hM SH 2阳性表达率为28.6%,分化程...目的:探讨人类错配修复基因hM LH 1、hM SH 2在肺癌组织中的表达及意义。方法:运用免疫组织化学S-P法对56例肺癌组织中hM LH 1、hM SH 2的表达进行检测。结果:56例肺癌组织中hM LH 1的阳性表达率为35%,hM SH 2阳性表达率为28.6%,分化程度高者阳性率显著高于分化程度低者(P<0.01),有淋巴结转移者hM LH 1及hM SH 2阳性率低于无淋巴结转移者(P<0.05),不同病理组织学类型之间hM LH 1及hM SH 2表达无显著差别(P>0.05)。结论:hM LH 1及hM SH 2基因的缺陷与肺癌的发生发展过程并与其分化程度及有否淋巴结转移有关。展开更多
目的:探讨hMSH2基因启动子区5'CpG岛高甲基化在胃癌发生过程中的作用.方法:应用甲基化特异性PCR(methylation specific PCR,MSP)方法检测胃癌及非癌组织中hMSH2基因启动子区甲基化状态.结果:40例胃癌中hMSH2基因启动子区高甲基化24...目的:探讨hMSH2基因启动子区5'CpG岛高甲基化在胃癌发生过程中的作用.方法:应用甲基化特异性PCR(methylation specific PCR,MSP)方法检测胃癌及非癌组织中hMSH2基因启动子区甲基化状态.结果:40例胃癌中hMSH2基因启动子区高甲基化24例(60%),其癌旁黏膜组织中有15例(37.5%)发生甲基化,14例慢性萎缩性胃炎组织中有5例(35.7%)发生甲基化,6例慢性浅表性胃炎组织中未见甲基化.四组甲基化水平相比,差别有统计意义(P<0.05).胃癌组甲基化水平高于癌旁组,差别有统计意义(P<0.05).癌旁组、慢性萎缩性胃炎组、慢性浅表性胃炎组三组甲基化水平相比,差别无统计意义.胃癌各临床病理参数组之间相比差别无统计意义.结论:胃癌组织中hMSH2基因启动子区高甲基化可能是导致其错配修复功能缺陷的重要原因之一;而错配修复功能缺陷在胃癌的发生中起着重要作用,但可能与其发展关系不大.展开更多
基金Supported by a grant from Chinese Scientific Academy Creative Foundation (No. DICPkaaaabc).
文摘Objective: To study the possibility of mismatch repair gene hMSH2 as a marker to predict the occurrence of gastric carcinoma. Methods: Immunohistochemical method was used to detect hMSH2 protein expressions of gastric carci- nomas (carcinoma group) and their surrounding epithelia (surrounding epithelium group) in patients and the epithelia (normal epithelium group) in persons without carcinoma. Results: The positive rates of hMSH2 protein expression in nucleus of carcinoma, surrounding epithelium and normal epithelium groups were 44.94% (71/158), 28.48% (45/158) and 11.76% (4/34), respectively (P=0.000); the positive rates of hMSH2 protein expression in plasma of the 3 groups were 37.97% (60/158), 27.85% (44/158) and 23.53% (8/34), respectively (P=0.084); the positive rates of hMSH2 protein expression in both nucleus and plasma of the 3 groups were 20.89% (33/158), 17.72% (28/158) and 2.94% (1/34), respectively (P=0.045). Although the difference of positive rates between carcinoma and surrounding epithelium groups was not significant (P=0.476), both of them were higher than that of normal epithelium group (P=0.018). Conclusion: Our findings show that the detection of hMSH2 protein expression in gastric epithelia may help to predict and diagnose gastric carcinoma.
文摘目的:探讨人类错配修复基因hM LH 1、hM SH 2在肺癌组织中的表达及意义。方法:运用免疫组织化学S-P法对56例肺癌组织中hM LH 1、hM SH 2的表达进行检测。结果:56例肺癌组织中hM LH 1的阳性表达率为35%,hM SH 2阳性表达率为28.6%,分化程度高者阳性率显著高于分化程度低者(P<0.01),有淋巴结转移者hM LH 1及hM SH 2阳性率低于无淋巴结转移者(P<0.05),不同病理组织学类型之间hM LH 1及hM SH 2表达无显著差别(P>0.05)。结论:hM LH 1及hM SH 2基因的缺陷与肺癌的发生发展过程并与其分化程度及有否淋巴结转移有关。
文摘目的:探讨hMSH2基因启动子区5'CpG岛高甲基化在胃癌发生过程中的作用.方法:应用甲基化特异性PCR(methylation specific PCR,MSP)方法检测胃癌及非癌组织中hMSH2基因启动子区甲基化状态.结果:40例胃癌中hMSH2基因启动子区高甲基化24例(60%),其癌旁黏膜组织中有15例(37.5%)发生甲基化,14例慢性萎缩性胃炎组织中有5例(35.7%)发生甲基化,6例慢性浅表性胃炎组织中未见甲基化.四组甲基化水平相比,差别有统计意义(P<0.05).胃癌组甲基化水平高于癌旁组,差别有统计意义(P<0.05).癌旁组、慢性萎缩性胃炎组、慢性浅表性胃炎组三组甲基化水平相比,差别无统计意义.胃癌各临床病理参数组之间相比差别无统计意义.结论:胃癌组织中hMSH2基因启动子区高甲基化可能是导致其错配修复功能缺陷的重要原因之一;而错配修复功能缺陷在胃癌的发生中起着重要作用,但可能与其发展关系不大.
